The Experts below are selected from a list of 2595 Experts worldwide ranked by ideXlab platform
Hongying Zhang - One of the best experts on this subject based on the ideXlab platform.
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primary cardiac Dedifferentiated Liposarcoma with homologous and heterologous differentiation a case report
International Journal of Clinical and Experimental Pathology, 2015Co-Authors: Du He, Min Chen, Huijiao Chen, Dianying Liao, Xiaozhou Wang, Zhang Zhang, Hongying ZhangAbstract:Liposarcoma originating in the heart is extraordinarily rare. Herein, we report a Dedifferentiated Liposarcoma arising from the left atrium in a 59-year-old Chinese man. Histologically, the neoplasm predominantly consisted of undifferentiated pleomorphic sarcoma. In addition, the neoplasm exhibited lipoblastic differentiation and osteo-/chondrosarcomatous components. Immunohistochemically, the neoplastic cells were strongly positive for p16, MDM2, and CDK4. Fluorescence in situ hybridization showed MDM2 gene amplification in all of the tumor components. To the best of our knowledge, this is the first published example of cardiac Dedifferentiated Liposarcoma exhibiting homologous and heterologous differentiation without a well-differentiated Liposarcoma component.
Lucia Casadei - One of the best experts on this subject based on the ideXlab platform.
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mdm2 derived from Dedifferentiated Liposarcoma extracellular vesicles induces mmp2 production from preadipocytes
Cancer Research, 2019Co-Authors: Lucia Casadei, Federica Calore, Danielle Braggio, Abeba Zewdu, Ameya Deshmukh, Paolo Fadda, Gonzalo LopezAbstract:Dedifferentiated Liposarcoma (DDLPS) is frequently diagnosed late, and patients typically respond poorly to treatments. DDLPS is molecularly characterized by wild-type p53 and amplification of the MDM2 gene, which results in overexpression of MDM2 protein, a key oncogenic process in DDLPS. In this study, we demonstrate that extracellular vesicles derived from patients with DDLPS or from DDLPS cell lines are carriers of MDM2 DNA that can be transferred to preadipocytes, a major and ubiquitous cellular component of the DDLPS tumor microenvironment, leading to impaired p53 activity in preadipocytes and increased proliferation, migration, and production of matrix metalloproteinase 2; treatment with MDM2 inhibitors repressed these effects. Overall, these findings indicate that MDM2 plays a crucial role in DDLPS by enabling cross-talk between tumor cells and the surrounding microenvironment and that targeting vesicular MDM2 could represent a therapeutic option for treating DDLPS. Significance: Extracellular vesicles derived from Dedifferentiated Liposarcoma cells induce oncogenic properties in preadipocytes.
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mdm2 derived from Dedifferentiated Liposarcoma extracellular vesicles induces mmp2 production from preadipocytes
Cancer Research, 2019Co-Authors: Lucia Casadei, Federica Calore, Danielle Braggio, Abeba Zewdu, Ameya Deshmukh, Paolo Fadda, Gonzalo LopezAbstract:Dedifferentiated Liposarcoma (DDLPS) is frequently diagnosed late, and patients typically respond poorly to treatments. DDLPS is molecularly characterized by wild-type p53 and amplification of the MDM2 gene, which results in overexpression of MDM2 protein, a key oncogenic process in DDLPS. In this study, we demonstrate that extracellular vesicles derived from patients with DDLPS or from DDLPS cell lines are carriers of MDM2 DNA that can be transferred to preadipocytes, a major and ubiquitous cellular component of the DDLPS tumor microenvironment, leading to impaired p53 activity in preadipocytes and increased proliferation, migration, and production of matrix metalloproteinase 2; treatment with MDM2 inhibitors repressed these effects. Overall, these findings indicate that MDM2 plays a crucial role in DDLPS by enabling cross-talk between tumor cells and the surrounding microenvironment and that targeting vesicular MDM2 could represent a therapeutic option for treating DDLPS. SIGNIFICANCE: Extracellular vesicles derived from Dedifferentiated Liposarcoma cells induce oncogenic properties in preadipocytes.
Baishali Bhattacharya - One of the best experts on this subject based on the ideXlab platform.
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Dedifferentiated Liposarcoma arising from the sigmoid mesocolon a case report
World Journal of Gastroenterology, 2007Co-Authors: Brody Winn, Homayoon Akbari, Baishali BhattacharyaAbstract:Dedifferentiated Liposarcoma is a variant of Liposarcoma with a more aggressive course. It occurs most commonly in the retroperitoneum and rarely in other anatomic locations. In the present report, we describe a case of Dedifferentiated Liposarcoma that occurred in an unusual location, sigmoid mesocolon, which has not yet been documented.
Takanori Hirose - One of the best experts on this subject based on the ideXlab platform.
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Establishment and characterization of a novel Dedifferentiated Liposarcoma cell line, NDDLS-1.
Pathology International, 2011Co-Authors: Takashi Ariizumi, Akira Ogose, Hiroyuki Kawashima, Tetsuo Hotta, Guidong Li, Yongjun Xu, Takanori Hirose, Naoto EndoAbstract:: We established a Dedifferentiated Liposarcoma cell line (NDDLS-1) that produces interleukin-6 (IL-6) and granulocyte-colony stimulating factor (G-CSF). The parental tumor showed high leukemoid reactions. The NDDLS-1 cell line was established from a pleural effusion associated with a lung metastasis. Pleomorphic tumor cells arranged in a haphazard growth pattern were seen in xenograft tumors. Numerous inflammatory cells including neutrophils or eosinophils were present throughout the tumor cells. This finding resembled the Dedifferentiated area of the parental tumor. The mice bearing NDDLS-1 showed marked leukocytosis. In addition, the NDDLS-1 cells expressed IL-6 and G-CSF at both the mRNA and protein levels, while the NDDLS-1 cells produced near normal levels of tumor necrosis factor alpha (TNF-α). In the cytogenetic analysis, both the parental tumor and the NDDLS-1 cells showed a ring or giant marker chromosomes. The NDDLS-1 cell line demonstrated the amplification and expression of both MDM2 and CDK4 by fluorescence in situ hybridization and immunohistochemical analysis. The NDDLS-1 cell line is consistent with the parental Dedifferentiated Liposarcoma, and it should therefore be useful for further investigations of human Dedifferentiated Liposarcomas.
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Dedifferentiated Liposarcoma with rhabdomyoblastic differentiation
Virchows Archiv, 2005Co-Authors: Shio Shimada, Takashi Ishizawa, Keisuke Ishizawa, Kouichi Kamada, Takanori HiroseAbstract:Dedifferentiated areas of Dedifferentiated Liposarcoma (DDL) usually show malignant fibrous histiocytoma (MFH)- or fibrosarcoma-like features and lack any histologic signs of specific differentiation. However, some reports have demonstrated specific differentiation in these areas, with histologic features resembling those of rhabdomyosarcoma, leiomyosarcoma, and osteosarcoma. We report here a pathologic and genetic analysis of three cases of DDLs with rhabdomyosarcomatous areas. MFH- or fibrosarcoma-like areas of one primary DDL and two recurrent DDLs contained various amounts of rhabdomyoblasts, which were immunoreactive for desmin, myoglobin, muscle actin (HHF-35), and myogenin. An ultrastructural examination demonstrated rhabdomyoblasts with abundant cytoplasm containing thin and thick filaments and Z-bands. By real-time PCR, amplification of mdm2 and cdk4 was confirmed in both well-differentiated and Dedifferentiated areas with rhabdomyoblasts of all cases. To our knowledge, only seven cases of DDLs with rhabdomyosarcomatous components have been reported, and furthermore, the genetic profiles of the rhabdomyosarcomatous components in DDLs have not been investigated. This study demonstrates that DDLs with rhabdomyosarcomatous areas have genetic alterations that are common to well-differentiated/Dedifferentiated Liposarcomas.
Gonzalo Lopez - One of the best experts on this subject based on the ideXlab platform.
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mdm2 derived from Dedifferentiated Liposarcoma extracellular vesicles induces mmp2 production from preadipocytes
Cancer Research, 2019Co-Authors: Lucia Casadei, Federica Calore, Danielle Braggio, Abeba Zewdu, Ameya Deshmukh, Paolo Fadda, Gonzalo LopezAbstract:Dedifferentiated Liposarcoma (DDLPS) is frequently diagnosed late, and patients typically respond poorly to treatments. DDLPS is molecularly characterized by wild-type p53 and amplification of the MDM2 gene, which results in overexpression of MDM2 protein, a key oncogenic process in DDLPS. In this study, we demonstrate that extracellular vesicles derived from patients with DDLPS or from DDLPS cell lines are carriers of MDM2 DNA that can be transferred to preadipocytes, a major and ubiquitous cellular component of the DDLPS tumor microenvironment, leading to impaired p53 activity in preadipocytes and increased proliferation, migration, and production of matrix metalloproteinase 2; treatment with MDM2 inhibitors repressed these effects. Overall, these findings indicate that MDM2 plays a crucial role in DDLPS by enabling cross-talk between tumor cells and the surrounding microenvironment and that targeting vesicular MDM2 could represent a therapeutic option for treating DDLPS. Significance: Extracellular vesicles derived from Dedifferentiated Liposarcoma cells induce oncogenic properties in preadipocytes.
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mdm2 derived from Dedifferentiated Liposarcoma extracellular vesicles induces mmp2 production from preadipocytes
Cancer Research, 2019Co-Authors: Lucia Casadei, Federica Calore, Danielle Braggio, Abeba Zewdu, Ameya Deshmukh, Paolo Fadda, Gonzalo LopezAbstract:Dedifferentiated Liposarcoma (DDLPS) is frequently diagnosed late, and patients typically respond poorly to treatments. DDLPS is molecularly characterized by wild-type p53 and amplification of the MDM2 gene, which results in overexpression of MDM2 protein, a key oncogenic process in DDLPS. In this study, we demonstrate that extracellular vesicles derived from patients with DDLPS or from DDLPS cell lines are carriers of MDM2 DNA that can be transferred to preadipocytes, a major and ubiquitous cellular component of the DDLPS tumor microenvironment, leading to impaired p53 activity in preadipocytes and increased proliferation, migration, and production of matrix metalloproteinase 2; treatment with MDM2 inhibitors repressed these effects. Overall, these findings indicate that MDM2 plays a crucial role in DDLPS by enabling cross-talk between tumor cells and the surrounding microenvironment and that targeting vesicular MDM2 could represent a therapeutic option for treating DDLPS. SIGNIFICANCE: Extracellular vesicles derived from Dedifferentiated Liposarcoma cells induce oncogenic properties in preadipocytes.
