The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform
Ren-xi Zhuo - One of the best experts on this subject based on the ideXlab platform.
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calcium phosphate dna co precipitates encapsulated fast Degrading Polymer films for substrate mediated gene delivery
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
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three dimensional fast Degrading Polymer films for delivery of calcium phosphate dna co precipitates in solid phase transfection
Journal of Materials Chemistry, 2009Co-Authors: Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:We prepared Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films for substrate-mediated gene delivery. The Polymer films were composed of fast-Degrading functionalized star poly(DL-lactide) CA-PDLLA and block coPolymer poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) (PLLA-PEG-PLLA). The in vitrogene transfections of pGL3-Luc and pEGFP-C1 plasmids in HEK293T cells mediated by different films were studied. The effect of presence of a water-soluble Polymer, poly-α,β-[N-(2-hydroxyethyl)-L-aspartamide], on the transfection activity of the film-mediated transfection was studied. The gene expressions could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films. During the cellular transfection, the fast-Degrading films rapidly released Ca-P/DNA co-precipitates to mediate gene transfection, and the degradation products did not show any negative effects on the gene expression. The Ca-P/DNA co-precipitates deposited and encapsulated in films have promising applications in substrate-mediated gene delivery.
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Calcium phosphate/DNA co-precipitates encapsulated fast-Degrading Polymer films for substrate-mediated gene delivery.
Journal of biomedical materials research. Part B Applied biomaterials, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
Xian-zheng Zhang - One of the best experts on this subject based on the ideXlab platform.
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calcium phosphate dna co precipitates encapsulated fast Degrading Polymer films for substrate mediated gene delivery
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
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three dimensional fast Degrading Polymer films for delivery of calcium phosphate dna co precipitates in solid phase transfection
Journal of Materials Chemistry, 2009Co-Authors: Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:We prepared Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films for substrate-mediated gene delivery. The Polymer films were composed of fast-Degrading functionalized star poly(DL-lactide) CA-PDLLA and block coPolymer poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) (PLLA-PEG-PLLA). The in vitrogene transfections of pGL3-Luc and pEGFP-C1 plasmids in HEK293T cells mediated by different films were studied. The effect of presence of a water-soluble Polymer, poly-α,β-[N-(2-hydroxyethyl)-L-aspartamide], on the transfection activity of the film-mediated transfection was studied. The gene expressions could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films. During the cellular transfection, the fast-Degrading films rapidly released Ca-P/DNA co-precipitates to mediate gene transfection, and the degradation products did not show any negative effects on the gene expression. The Ca-P/DNA co-precipitates deposited and encapsulated in films have promising applications in substrate-mediated gene delivery.
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Calcium phosphate/DNA co-precipitates encapsulated fast-Degrading Polymer films for substrate-mediated gene delivery.
Journal of biomedical materials research. Part B Applied biomaterials, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
Qiao Zhang - One of the best experts on this subject based on the ideXlab platform.
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calcium phosphate dna co precipitates encapsulated fast Degrading Polymer films for substrate mediated gene delivery
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
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Calcium phosphate/DNA co-precipitates encapsulated fast-Degrading Polymer films for substrate-mediated gene delivery.
Journal of biomedical materials research. Part B Applied biomaterials, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
Si-xue Cheng - One of the best experts on this subject based on the ideXlab platform.
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calcium phosphate dna co precipitates encapsulated fast Degrading Polymer films for substrate mediated gene delivery
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
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three dimensional fast Degrading Polymer films for delivery of calcium phosphate dna co precipitates in solid phase transfection
Journal of Materials Chemistry, 2009Co-Authors: Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:We prepared Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films for substrate-mediated gene delivery. The Polymer films were composed of fast-Degrading functionalized star poly(DL-lactide) CA-PDLLA and block coPolymer poly(L-lactide)-poly(ethylene glycol)-poly(L-lactide) (PLLA-PEG-PLLA). The in vitrogene transfections of pGL3-Luc and pEGFP-C1 plasmids in HEK293T cells mediated by different films were studied. The effect of presence of a water-soluble Polymer, poly-α,β-[N-(2-hydroxyethyl)-L-aspartamide], on the transfection activity of the film-mediated transfection was studied. The gene expressions could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated in Polymer films. During the cellular transfection, the fast-Degrading films rapidly released Ca-P/DNA co-precipitates to mediate gene transfection, and the degradation products did not show any negative effects on the gene expression. The Ca-P/DNA co-precipitates deposited and encapsulated in films have promising applications in substrate-mediated gene delivery.
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Calcium phosphate/DNA co-precipitates encapsulated fast-Degrading Polymer films for substrate-mediated gene delivery.
Journal of biomedical materials research. Part B Applied biomaterials, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
Dong Zhao - One of the best experts on this subject based on the ideXlab platform.
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calcium phosphate dna co precipitates encapsulated fast Degrading Polymer films for substrate mediated gene delivery
Journal of Biomedical Materials Research Part B, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.
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Calcium phosphate/DNA co-precipitates encapsulated fast-Degrading Polymer films for substrate-mediated gene delivery.
Journal of biomedical materials research. Part B Applied biomaterials, 2009Co-Authors: Qiao Zhang, Dong Zhao, Xian-zheng Zhang, Si-xue Cheng, Ren-xi ZhuoAbstract:Calcium-phosphate/deoxyribose nucleic acid (Ca-P/DNA) co-precipitates were deposited on or encapsulated in fast-Degrading Polymer films with surface erosion degradation mechanism to mediate cell transfection. The Polymer, cholic acid functionalized star poly(DL-lactide), was synthesized through the ring-opening Polymerization of DL-lactide initiated by cholic acid. The releases of DNA from the Ca-P/DNA co-precipitates deposited film and the Ca-P/DNA co-precipitates encapsulated film were determined and compared. The in vitro gene transfections of HEK293 cells, Hela cells, and NIH 3T3 cells showed that the expression of pGL3-Luc plasmid could be effectively mediated by the Ca-P/DNA co-precipitates deposited and encapsulated Polymer films. In addition, the films did not exhibit any additional cytotoxicity to the cells during the transfections, indicating that the fast-Degrading Polymer films have great potential in localized gene delivery.