The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Liangping Ye - One of the best experts on this subject based on the ideXlab platform.
-
terminal complement complex c5b 9 reduced megalin and cubilin mediated tubule proteins uptake in a mouse model of trichloroethylene hypersensitivity syndrome
Toxicology Letters, 2019Co-Authors: Feng Wang, Liping Huang, Meng Huang, Wei Jiang, Liangping YeAbstract:Abstract Trichloroethylene (TCE), a commonly used industrial solvent and Degreasing Agent, is known to cause trichloroethylene hypersensitivity syndrome (THS) with multi-system damage, including skin, liver and kidney. Clinical evidence have shown that the kidney injury occurs in THS and our previous studies suggested that the terminal complement complex C5b-9 deposited in impaired renal tubules induced by TCE with unclear mechanisms. In the present study, we questioned whether activation of the complement system with renal deposition of C5b-9 contributes to TCE-induced kidney injury in THS. We established a BALB/c mouse model of TCE sensitization with or without pretreatment of exogenous CD59, a C5b-9 inhibitory protein. H&E staining, PAS staining, and biochemical detection of urinary proteins were performed to assess renal function. Deposition of C5b-9 and expression of CD59 were evaluated by immunohistochemistry. Sub-lytic effects of C5b-9 in tubular epithelial cells were assessed by lactate dehydrogenase (LDH) cytotoxicity assay. Expression of endocytosis receptors megalin and cubilin on proximal tubules were assessed by immunofluorescence and qRT-PCR. We found that TCE sensitization induced structural and functional changes of renal tubules in mice, associated with the deposition of sub-lytic C5b-9 on proximal tubular epithelial cells. TCE sensitization decreased proximal tubule uptake of filtered proteins and renal expression of megalin and cubilin, phenotypes that were attenuated by pretreatment with exogenous CD59. Overall, our findings reveal a novel mechanism underlying sub-lytic C5b-9 acting on megalin and cubilin, contributes to the renal tubules damage by TCE exposure.
Masayuki Ikeda - One of the best experts on this subject based on the ideXlab platform.
-
Types of organic solvents used in small- to medium-scale industries in Japan; a nationwide field survey
International Archives of Occupational and Environmental Health, 1997Co-Authors: Hirohiko Ukai, Shunen Inui, Shiro Takada, Junko Dendo, Junichi Ogawa, Kiyofusa Isobe, Toshifumi Ashida, Mikio Tamura, Koshiro Tabuki, Masayuki IkedaAbstract:Objective : The aim of the present survey is to identify organic solvents commonly used in various workplaces in Japan. Methods : A total of 24 occupational health service institutions (OHSI) distributed nationwide in Japan offered data on types of solvent workplaces, types of solvents used therein, and the solvent concentrations surveyed in a 2-month period between April and May 1996 to form a data base (OHSI data base, consisting of 1597 cases). Separately, Kyoto Industrial Health Association (KIHA) offered information on 948 cases studied during a 1-year period ranging from April 1995 to March 1996 (KIHA data base). The two data bases were treated in parallel to examine the reproducibility of the results. Results : Detection prevalence was very low (0–1%) for almost half of the 47 legally regulated solvents. Among the solvents in use, toluene was most frequently detected, although the prevalence appeared to be reduced as compared with that recorded for the early 1980s. The most frequently observed solvent combinations comprised toluene, xylenes, and ethyl acetate in the OHSI data base and toluene, xylenes, and methanol in the KIHA data base. Contrary to the case in the 1980s, dichloromethane was used more often than trichloroethylene as a Degreasing Agent in the present survey. No use was detected for carbon tetrachloride, chloroform, 1,2-dichloroethane, 1,2-dichloroethylene, or 1,1,2,2-tetrachloroethane except for research purposes. Conclusions : Toluene remained the most common solvent and was used in combination with xylenes, ethyl acetate, and methanol. There was an increase in the use of dichloromethane as a Degreasing Agent.
H.j. Styhr Petersen - One of the best experts on this subject based on the ideXlab platform.
-
Formulation and Recycling of Water-Based Degreasing Agents: Effects on Occupational Health and Environmental Protection
Hazardous waste and hazardous materials, 1994Co-Authors: Frode Sørensen, H.j. Styhr PetersenAbstract:ABSTRACT In metal Degreasing, aqueous alkaline solutions can often be substituted for organic solvents. Such water-based Degreasing Agents should be formulated so that associated negative impacts on occupational health and the environment are minimized. With these goals in mind, a review and an evaluation of chemicals utilization in water-based Degreasing Agents has been carried out. These include strongly alkaline Agents, sodium metasilicate, complexing Agents, corrosion inhibitors, surfactants, organic solvents, bactericides, coloring Agents, and perfumes. In a shop test a water-based Degreasing Agent formulated in accordance with these criteria has been shown to be able to degrease aluminum parts contaminated with oils from a draw process to an acceptable quality of cleanness before burnishing. By recycling the Degreasing Agent in a microfiltration unit it was possible to increase the service life of the bath 5 times, and to reduce the use of chemicals such as the builder component to 20%, the surfacta...
Yun Chen - One of the best experts on this subject based on the ideXlab platform.
-
stainless steel cleaner and stainless steel cleaning method
2011Co-Authors: Mingjie Sha, Pei Ding, Liusen Wu, Fuli Zhang, Yun ChenAbstract:The invention belongs to the field of surface treatment, and relates to a stainless steel cleaner. The cleaner comprises a nonionic surfactant, an anionic surfactant, a complexing Agent, a Degreasing Agent and deionized water, wherein the Degreasing Agent is one of amide, and a mixture of amide and hydramine. An environmentally-friendly cleaner comprising the amide or the hydramine, a composite surfactant and the like removes grease under the alkalescent condition, and has good cleaning effect due to the permeation effect of the composite surfactant on the grease and an oxide layer. The invention also relates to a stainless steel cleaning method. The stainless steel cleaner and the stainless steel cleaning method are widely applied to the treatment of various stainless steel surfaces.
Feng Wang - One of the best experts on this subject based on the ideXlab platform.
-
terminal complement complex c5b 9 reduced megalin and cubilin mediated tubule proteins uptake in a mouse model of trichloroethylene hypersensitivity syndrome
Toxicology Letters, 2019Co-Authors: Feng Wang, Liping Huang, Meng Huang, Wei Jiang, Liangping YeAbstract:Abstract Trichloroethylene (TCE), a commonly used industrial solvent and Degreasing Agent, is known to cause trichloroethylene hypersensitivity syndrome (THS) with multi-system damage, including skin, liver and kidney. Clinical evidence have shown that the kidney injury occurs in THS and our previous studies suggested that the terminal complement complex C5b-9 deposited in impaired renal tubules induced by TCE with unclear mechanisms. In the present study, we questioned whether activation of the complement system with renal deposition of C5b-9 contributes to TCE-induced kidney injury in THS. We established a BALB/c mouse model of TCE sensitization with or without pretreatment of exogenous CD59, a C5b-9 inhibitory protein. H&E staining, PAS staining, and biochemical detection of urinary proteins were performed to assess renal function. Deposition of C5b-9 and expression of CD59 were evaluated by immunohistochemistry. Sub-lytic effects of C5b-9 in tubular epithelial cells were assessed by lactate dehydrogenase (LDH) cytotoxicity assay. Expression of endocytosis receptors megalin and cubilin on proximal tubules were assessed by immunofluorescence and qRT-PCR. We found that TCE sensitization induced structural and functional changes of renal tubules in mice, associated with the deposition of sub-lytic C5b-9 on proximal tubular epithelial cells. TCE sensitization decreased proximal tubule uptake of filtered proteins and renal expression of megalin and cubilin, phenotypes that were attenuated by pretreatment with exogenous CD59. Overall, our findings reveal a novel mechanism underlying sub-lytic C5b-9 acting on megalin and cubilin, contributes to the renal tubules damage by TCE exposure.