The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform

Richard L Gregory - One of the best experts on this subject based on the ideXlab platform.

  • the impact of nicotine and cigarette smoke condensate on metabolic activity and biofilm formation of candida albicans on acrylic Denture Material
    Journal of Prosthodontics, 2020
    Co-Authors: Yasmin Mohammed Alzayer, Grace F Gomez, George J Eckert, John A Levon, Richard L Gregory
    Abstract:

    Purpose Smokers have increased Denture stomatitis caused primarily by Candida albicans. The primary aim of this study was to demonstrate the impact of a wide range of nicotine and cigarette smoke condensate (CSC) concentrations on biofilm formation and metabolic activity of C. albicans on acrylic Denture Material. Materials and methods C. albicans (ATCC strain 10231) was used. Standardized Denture acrylic (PMMA) specimens (total of 135 specimens) were incubated with C. albicans and exposed to nicotine and CSC at different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/ml) and (0, 0.25, 0.5, 1, 2, and 4 mg/ml), respectively. For each experiment, 3 samples per nicotine and CSC concentration and a total of 45 specimens (27 specimens for the nicotine and 18 specimens for the CSC-treated samples) were used and were selected randomly for each group. The control group consisted of 0 mg/ml of nicotine or CSC. The viability of C. albicans was measured using spiral plating on blood agar plates. The effect of nicotine and CSC concentrations on planktonic cells was were measured using a microplate reader. Metabolic activity of 24-hour-old established C. albicans biofilm exposed to nicotine and CSC for 24 hours in microtiter plates was determined using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT) reduction assay. Results The viability of C. albicans increased concomitant with increasing concentrations of CSC and nicotine, particularly at 0.5 and 2 mg/ml, respectively. Concentrations of CSC and nicotine above this resulted in an inhibitory effect on C. albicans viability. CSC and nicotine at 4 and 16 mg/ml, respectively, increased C. albicans biofilm metabolic activity. Conclusion Nicotine and CSC up to certain concentrations caused increases in biofilm formation, metabolic activity, viability, and planktonic cell absorbance of C. albicans. This in vitro study demonstrates the effectiveness of tobacco on promoting the growth of C. albicans and suggests their potential contributing factor in C. albicans biofilm related infections in smokers.

  • The Impact of Nicotine and Cigarette Smoke Condensate on Metabolic Activity and Biofilm Formation of Candida albicans on Acrylic Denture Material.
    Journal of Prosthodontics, 2018
    Co-Authors: Yasmin Mohammed Alzayer, Grace F Gomez, George J Eckert, John A Levon, Richard L Gregory
    Abstract:

    PURPOSE: Smokers have increased Denture stomatitis caused primarily by Candida albicans. The primary aim of this study was to demonstrate the impact of a wide range of nicotine and cigarette smoke condensate (CSC) concentrations on biofilm formation and metabolic activity of C. albicans on acrylic Denture Material. MaterialS AND METHODS: C. albicans (ATCC strain 10231) was used. Standardized Denture acrylic (PMMA) specimens (total of 135 specimens) were incubated with C. albicans and exposed to nicotine and CSC at different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/ml) and (0, 0.25, 0.5, 1, 2, and 4 mg/ml), respectively. For each experiment, 3 samples per nicotine and CSC concentration and a total of 45 specimens (27 specimens for the nicotine and 18 specimens for the CSC-treated samples) were used and were selected randomly for each group. The control group consisted of 0 mg/ml of nicotine or CSC. The viability of C. albicans was measured using spiral plating on blood agar plates. The effect of nicotine and CSC concentrations on planktonic cells was were measured using a microplate reader. Metabolic activity of 24-hour-old established C. albicans biofilm exposed to nicotine and CSC for 24 hours in microtiter plates was determined using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT) reduction assay. RESULTS: The viability of C. albicans increased concomitant with increasing concentrations of CSC and nicotine, particularly at 0.5 and 2 mg/ml, respectively. Concentrations of CSC and nicotine above this resulted in an inhibitory effect on C. albicans viability. CSC and nicotine at 4 and 16 mg/ml, respectively, increased C. albicans biofilm metabolic activity. CONCLUSION: Nicotine and CSC up to certain concentrations caused increases in biofilm formation, metabolic activity, viability, and planktonic cell absorbance of C. albicans. This in vitro study demonstrates the effectiveness of tobacco on promoting the growth of C. albicans and suggests their potential contributing factor in C. albicans biofilm related infections in smokers.

Yasmin Mohammed Alzayer - One of the best experts on this subject based on the ideXlab platform.

  • the impact of nicotine and cigarette smoke condensate on metabolic activity and biofilm formation of candida albicans on acrylic Denture Material
    Journal of Prosthodontics, 2020
    Co-Authors: Yasmin Mohammed Alzayer, Grace F Gomez, George J Eckert, John A Levon, Richard L Gregory
    Abstract:

    Purpose Smokers have increased Denture stomatitis caused primarily by Candida albicans. The primary aim of this study was to demonstrate the impact of a wide range of nicotine and cigarette smoke condensate (CSC) concentrations on biofilm formation and metabolic activity of C. albicans on acrylic Denture Material. Materials and methods C. albicans (ATCC strain 10231) was used. Standardized Denture acrylic (PMMA) specimens (total of 135 specimens) were incubated with C. albicans and exposed to nicotine and CSC at different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/ml) and (0, 0.25, 0.5, 1, 2, and 4 mg/ml), respectively. For each experiment, 3 samples per nicotine and CSC concentration and a total of 45 specimens (27 specimens for the nicotine and 18 specimens for the CSC-treated samples) were used and were selected randomly for each group. The control group consisted of 0 mg/ml of nicotine or CSC. The viability of C. albicans was measured using spiral plating on blood agar plates. The effect of nicotine and CSC concentrations on planktonic cells was were measured using a microplate reader. Metabolic activity of 24-hour-old established C. albicans biofilm exposed to nicotine and CSC for 24 hours in microtiter plates was determined using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT) reduction assay. Results The viability of C. albicans increased concomitant with increasing concentrations of CSC and nicotine, particularly at 0.5 and 2 mg/ml, respectively. Concentrations of CSC and nicotine above this resulted in an inhibitory effect on C. albicans viability. CSC and nicotine at 4 and 16 mg/ml, respectively, increased C. albicans biofilm metabolic activity. Conclusion Nicotine and CSC up to certain concentrations caused increases in biofilm formation, metabolic activity, viability, and planktonic cell absorbance of C. albicans. This in vitro study demonstrates the effectiveness of tobacco on promoting the growth of C. albicans and suggests their potential contributing factor in C. albicans biofilm related infections in smokers.

  • The Impact of Nicotine and Cigarette Smoke Condensate on Metabolic Activity and Biofilm Formation of Candida albicans on Acrylic Denture Material.
    Journal of Prosthodontics, 2018
    Co-Authors: Yasmin Mohammed Alzayer, Grace F Gomez, George J Eckert, John A Levon, Richard L Gregory
    Abstract:

    PURPOSE: Smokers have increased Denture stomatitis caused primarily by Candida albicans. The primary aim of this study was to demonstrate the impact of a wide range of nicotine and cigarette smoke condensate (CSC) concentrations on biofilm formation and metabolic activity of C. albicans on acrylic Denture Material. MaterialS AND METHODS: C. albicans (ATCC strain 10231) was used. Standardized Denture acrylic (PMMA) specimens (total of 135 specimens) were incubated with C. albicans and exposed to nicotine and CSC at different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, and 32 mg/ml) and (0, 0.25, 0.5, 1, 2, and 4 mg/ml), respectively. For each experiment, 3 samples per nicotine and CSC concentration and a total of 45 specimens (27 specimens for the nicotine and 18 specimens for the CSC-treated samples) were used and were selected randomly for each group. The control group consisted of 0 mg/ml of nicotine or CSC. The viability of C. albicans was measured using spiral plating on blood agar plates. The effect of nicotine and CSC concentrations on planktonic cells was were measured using a microplate reader. Metabolic activity of 24-hour-old established C. albicans biofilm exposed to nicotine and CSC for 24 hours in microtiter plates was determined using a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-carboxanilide (XTT) reduction assay. RESULTS: The viability of C. albicans increased concomitant with increasing concentrations of CSC and nicotine, particularly at 0.5 and 2 mg/ml, respectively. Concentrations of CSC and nicotine above this resulted in an inhibitory effect on C. albicans viability. CSC and nicotine at 4 and 16 mg/ml, respectively, increased C. albicans biofilm metabolic activity. CONCLUSION: Nicotine and CSC up to certain concentrations caused increases in biofilm formation, metabolic activity, viability, and planktonic cell absorbance of C. albicans. This in vitro study demonstrates the effectiveness of tobacco on promoting the growth of C. albicans and suggests their potential contributing factor in C. albicans biofilm related infections in smokers.

Zhaohua Zhong - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of chitosan quaternary ammonium salt modified resin Denture base Material
    International Journal of Biological Macromolecules, 2016
    Co-Authors: Rong Song, Zhaohua Zhong
    Abstract:

    Chitosan quaternary ammonium salt displays good antioxidant and antibacterial characteristics and it shows appreciable solubility in water. When added to the traditional Denture Material to form a resin base, it could promote good oral health by improving the oral environment. In this study, chitosan quaternary ammonium salt was added to the Denture Material following two different methods. After three months of immersion in artificial saliva, the specimens were tested for tensile strength and were scanned by electron microscope. The murine fibroblast cytotoxicity and antibacterial properties were also tested. The result showed no significant differences in the tensile strength and in the proliferation of murine L929 fibroblast cells. The two structures of chitosan quaternary ammonium salt-modified Denture Material had different degrees of corrosion resistance and antimicrobial properties. These results indicate that chitosan quaternary ammonium salt-modified resin Denture base Material has the potential to become a new generation oral Denture composite Material.

Huaiqin Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Effect of oxygen plasma treatment on the bonding of a soft liner to an acrylic resin Denture Material.
    Dental Materials Journal, 2010
    Co-Authors: Huaiqin Zhang, Jianglin Fang, Zheng Hu, Junchi Ma, Jie Bian
    Abstract:

    Mechanical roughening reportedly had a weakening effect on bond strength. Therefore, the purpose of this study was to evaluate the effect of an alternative surface roughening method, namely oxygen plasma treatment, on the tensile bond strength between Denture base resin and soft liner. The soft liner used in this study was Soft Reverse, whilst the Denture base Material was Zi Ran. Three groups of specimens were prepared, comprising untreated specimens and oxygen plasma-treated specimens with exposure to air for 1 day and 2 days. All specimens were subjected to surface composition analysis and tensile bond strength testing. All data were analyzed using one-way ANOVA, and their mean values were compared using Tukey’s HSD test (p

  • effect of oxygen plasma treatment on the bonding of a soft liner to an acrylic resin Denture Material
    Dental Materials Journal, 2010
    Co-Authors: Huaiqin Zhang, Jianglin Fang, Zheng Hu, Jie Bian
    Abstract:

    Mechanical roughening reportedly had a weakening effect on bond strength. Therefore, the purpose of this study was to evaluate the effect of an alternative surface roughening method, namely oxygen plasma treatment, on the tensile bond strength between Denture base resin and soft liner. The soft liner used in this study was Soft Reverse, whilst the Denture base Material was Zi Ran. Three groups of specimens were prepared, comprising untreated specimens and oxygen plasma-treated specimens with exposure to air for 1 day and 2 days. All specimens were subjected to surface composition analysis and tensile bond strength testing. All data were analyzed using one-way ANOVA, and their mean values were compared using Tukey’s HSD test (p<0.01). Highest tensile bond strength was observed in the 1-day exposure group (5.2 MPa), whilst the lowest in the control group of untreated specimens (2.8 MPa). Hence, results of this study clearly indicated that oxygen plasma treatment was effective in enhancing tensile bond strength.

  • Effects of plasma treatment time on modification of acrylic Denture Material
    Journal of Nanjing Medical University, 2007
    Co-Authors: Huaiqin Zhang, Jianglin Fang
    Abstract:

    Abstract Objective To study the relationship between plasma treatment time and efficacy. Methods Test specimens were prepared from an acrylic resin Denture Material in the size of 2 mm × 10 mm × 10 mm. Plasma treatment was carried out on the surface of Polymethyl methacrylate(PMMA) at different time. XPS studies, IR spectra studies and measurement of wetting angle were performed. Results XPS showed the peak corresponding to C-O getting higher as the treatment proceeded, however at 120 seconds, the peak did not increase any longer and partly crossed with the peak at the duration of 60 seconds. IR spectra showed the wave corresponding to C-H was reduced as O2-plasma treatment proceeded, and then changed little. Wetting angle initially decreased dramatically, however, as the reaction proceeded, wetting angle increased slightly. Conclusion Equilibrium was reached for introducing oxygen-containing groups and changing of C-H. As the treatment proceeded, wetting angle increased slightly.

Jie Bian - One of the best experts on this subject based on the ideXlab platform.

  • Effect of oxygen plasma treatment on the bonding of a soft liner to an acrylic resin Denture Material.
    Dental Materials Journal, 2010
    Co-Authors: Huaiqin Zhang, Jianglin Fang, Zheng Hu, Junchi Ma, Jie Bian
    Abstract:

    Mechanical roughening reportedly had a weakening effect on bond strength. Therefore, the purpose of this study was to evaluate the effect of an alternative surface roughening method, namely oxygen plasma treatment, on the tensile bond strength between Denture base resin and soft liner. The soft liner used in this study was Soft Reverse, whilst the Denture base Material was Zi Ran. Three groups of specimens were prepared, comprising untreated specimens and oxygen plasma-treated specimens with exposure to air for 1 day and 2 days. All specimens were subjected to surface composition analysis and tensile bond strength testing. All data were analyzed using one-way ANOVA, and their mean values were compared using Tukey’s HSD test (p

  • effect of oxygen plasma treatment on the bonding of a soft liner to an acrylic resin Denture Material
    Dental Materials Journal, 2010
    Co-Authors: Huaiqin Zhang, Jianglin Fang, Zheng Hu, Jie Bian
    Abstract:

    Mechanical roughening reportedly had a weakening effect on bond strength. Therefore, the purpose of this study was to evaluate the effect of an alternative surface roughening method, namely oxygen plasma treatment, on the tensile bond strength between Denture base resin and soft liner. The soft liner used in this study was Soft Reverse, whilst the Denture base Material was Zi Ran. Three groups of specimens were prepared, comprising untreated specimens and oxygen plasma-treated specimens with exposure to air for 1 day and 2 days. All specimens were subjected to surface composition analysis and tensile bond strength testing. All data were analyzed using one-way ANOVA, and their mean values were compared using Tukey’s HSD test (p<0.01). Highest tensile bond strength was observed in the 1-day exposure group (5.2 MPa), whilst the lowest in the control group of untreated specimens (2.8 MPa). Hence, results of this study clearly indicated that oxygen plasma treatment was effective in enhancing tensile bond strength.