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Pio Conti - One of the best experts on this subject based on the ideXlab platform.

  • 4 Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, A Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P<0.01) and in supernatant fluids (P<0.05) from minced granuloma, while IL-6 was inhibited in the supernatant fluids (P<0.05) of minced granulomas but was not detected in the serum of treated and untreated mice. In this study we show for the first time the antiinflammatory effect of 4-DPD on chronic inflammation and the inhibitory effect of TNF and IL-6 generation in supernatant fluids from minced granulomas.

  • 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganatein vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, Anthony Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO_4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO_4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P

Stavros Fridas - One of the best experts on this subject based on the ideXlab platform.

  • 4 Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, A Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P<0.01) and in supernatant fluids (P<0.05) from minced granuloma, while IL-6 was inhibited in the supernatant fluids (P<0.05) of minced granulomas but was not detected in the serum of treated and untreated mice. In this study we show for the first time the antiinflammatory effect of 4-DPD on chronic inflammation and the inhibitory effect of TNF and IL-6 generation in supernatant fluids from minced granulomas.

  • 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganatein vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, Anthony Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO_4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO_4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P

Christel Drewke - One of the best experts on this subject based on the ideXlab platform.

  • influence of antivitamins ginkgotoxin 5 phosphate and Deoxypyridoxine 5 phosphate on human pyridoxine 5 phosphate oxidase
    Planta Medica, 2009
    Co-Authors: Nora Salamon, Cristian Gurgui, Eckhard Leistner, Christel Drewke
    Abstract:

    The pharmacological effects of leaf extracts (EGb 761) from GINKGO BILOBA L. are attributed to ginkgolides, bilobalide and biflavonoids. However, besides these beneficial attributes, ginkgotoxin, a B 6 antivitamin which may cause epileptic convulsions, other severe neuronal disorders and even death, is also found in GINKGO leaves and leaf-derived remedies. Because of its structural similarity to the B 6 vitamers, an interaction of ginkgotoxin with enzymes involved in the vitamin B 6 -dependent metabolism of the human brain is possible. This led us to investigate how the neurotoxic ginkgotoxin acts in the brain. To this end the gene coding for the human pyridoxine 5′-phosphate oxidase was heterologously overexpressed in E. COLI and the homogeneous enzyme was characterized. The investigation showed that the enzyme is inhibited IN VITRO by the synthetic vitamin B 6 derivative 4′-Deoxypyridoxine 5′-phosphate but not by ginkgotoxin or its 5′-phosphate.

Eleni Dotsika - One of the best experts on this subject based on the ideXlab platform.

  • 4 Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, A Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P<0.01) and in supernatant fluids (P<0.05) from minced granuloma, while IL-6 was inhibited in the supernatant fluids (P<0.05) of minced granulomas but was not detected in the serum of treated and untreated mice. In this study we show for the first time the antiinflammatory effect of 4-DPD on chronic inflammation and the inhibitory effect of TNF and IL-6 generation in supernatant fluids from minced granulomas.

  • 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganatein vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, Anthony Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO_4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO_4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P

Christos Himonas - One of the best experts on this subject based on the ideXlab platform.

  • 4 Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganate in vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, A Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P<0.01) and in supernatant fluids (P<0.05) from minced granuloma, while IL-6 was inhibited in the supernatant fluids (P<0.05) of minced granulomas but was not detected in the serum of treated and untreated mice. In this study we show for the first time the antiinflammatory effect of 4-DPD on chronic inflammation and the inhibitory effect of TNF and IL-6 generation in supernatant fluids from minced granulomas.

  • 4-Deoxypyridoxine inhibits chronic granuloma formation induced by potassium permanganatein vivo
    Molecular and Cellular Biochemistry, 1994
    Co-Authors: Stavros Fridas, Anthony Trakatellis, Evdokia Karagouni, Eleni Dotsika, Christos Himonas, Pio Conti
    Abstract:

    4-Deoxypyridoxine (4-DPD) is a potent antagonist of Vitamin B6 coenzyme which inhibits IL-1, lymphocyte proliferation and has demonstrated that tollerance to skin grafts can be induced by administering splenic cells to pyridoxine-deficient mice. Chronic inflammation induced by dorsal injections of 200 μl of a 1∶40 saturated crystal solution of potassium permanganate (KMnO_4) in mice treated or untreated with 4-DPD (400 μg/dose), has been investigated. After 7 days all mice developed a subcutaneous granulomatous tissue indicative of a chronic inflammatory response, at the site of injection. KMnO_4-treated mice injected intraperitoneally with 4-DPD (400 μg/dose) on 5 consecutive days (the first at the same time of induction of the granuloma) show a significant decrease in size and weight of granuloma when compared to mice not treated with 4-DPD (Controls). In addition, in all mice treated with 4-DPD there was a strong inhibition of TNFα in serum (P