The Experts below are selected from a list of 1371 Experts worldwide ranked by ideXlab platform
Francine Puvion-dutilleul - One of the best experts on this subject based on the ideXlab platform.
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Printed in Great Britain © Company of Biologists Limited NON-NUCLEOLAR TRANSCRIPTION COMPLEXES OF RAT LIVER AS REVEALED BY SPREADING ISOLATED NUCLEI
2015Co-Authors: Francis Harper, Francine Puvion-dutilleulAbstract:Miller's technique was applied to isolated nuclei of rat liver. Both the usual nucleolar and non-nucleolar transcription complexes were visualized. In addition, an unusual type of putative non-ribosomal transcription unit was revealed. It was characterized by a high density of the lateral ribonucleoprotein (RNP) fibrils. Although these particular units exhibited a regular increase of fibril lengths, the length of the transcript-covered Deoxyribonucleoprotein (DNP) fibres and the morphological aspect of the RNP fibrils distinguished them from the nucleolar ' Christmas-tree '-like figures. The linear and granular configuration of the transcripts and the absence of terminal knobs made them similar to non-nucleolar nascent RNP fibrils
J. A. V. Butler - One of the best experts on this subject based on the ideXlab platform.
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Fractionation of Deoxyribonucleoprotein
Bulletin des Sociétés Chimiques Belges, 2010Co-Authors: J. A. Lucy, J. A. V. ButlerAbstract:1 The extraction of denatured desoxyribonucleoprotein, from calf thymus, with successive quantities of 0.6 N sodium chloride solution has been found to yield nucleic acid fractions (DNA) with varying base ratios, the initial extracts being richer in guanine and cytosine. 2 Some physical-chemical properties of these DNA fractions have been determined. 3 The histones extracted by this means have also been examined and it has been found that the earlier fractions show a relatively high content of lysine, while the later fractions are richer in arginine. However, the lysine rich histone is apparently not firmly attached to the DNA and may have been extracted independently.
Francis Harper - One of the best experts on this subject based on the ideXlab platform.
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Printed in Great Britain © Company of Biologists Limited NON-NUCLEOLAR TRANSCRIPTION COMPLEXES OF RAT LIVER AS REVEALED BY SPREADING ISOLATED NUCLEI
2015Co-Authors: Francis Harper, Francine Puvion-dutilleulAbstract:Miller's technique was applied to isolated nuclei of rat liver. Both the usual nucleolar and non-nucleolar transcription complexes were visualized. In addition, an unusual type of putative non-ribosomal transcription unit was revealed. It was characterized by a high density of the lateral ribonucleoprotein (RNP) fibrils. Although these particular units exhibited a regular increase of fibril lengths, the length of the transcript-covered Deoxyribonucleoprotein (DNP) fibres and the morphological aspect of the RNP fibrils distinguished them from the nucleolar ' Christmas-tree '-like figures. The linear and granular configuration of the transcripts and the absence of terminal knobs made them similar to non-nucleolar nascent RNP fibrils
J. A. Lucy - One of the best experts on this subject based on the ideXlab platform.
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Fractionation of Deoxyribonucleoprotein
Bulletin des Sociétés Chimiques Belges, 2010Co-Authors: J. A. Lucy, J. A. V. ButlerAbstract:1 The extraction of denatured desoxyribonucleoprotein, from calf thymus, with successive quantities of 0.6 N sodium chloride solution has been found to yield nucleic acid fractions (DNA) with varying base ratios, the initial extracts being richer in guanine and cytosine. 2 Some physical-chemical properties of these DNA fractions have been determined. 3 The histones extracted by this means have also been examined and it has been found that the earlier fractions show a relatively high content of lysine, while the later fractions are richer in arginine. However, the lysine rich histone is apparently not firmly attached to the DNA and may have been extracted independently.
E.l. Peters - One of the best experts on this subject based on the ideXlab platform.
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Deoxyribonucleoprotein structure and radiation injury: cellular radiosensitivity is determined by LET infinity -dependent DNA damage in hydrated Deoxyribonucleoproteins and the extent of its repair.
Advances in space research : the official journal of the Committee on Space Research (COSPAR), 1992Co-Authors: J.t. Lett, E.l. PetersAbstract:Abstract For decades, theories of cellular radiosensitivity relied upon the initial patterns of energy deposition to explain radiation lethality. Such theories are unsound: cellular (DNA) repair also underlies cellular radiosensitivity. For the charged particles encountered in deep space, both the types of DNA damage caused in cellular Deoxyribonucleoproteins and the efficacies of their repair are dependent on linear energy transfer (LET ∞ ), and repair efficiency is also influenced by cell and tissue type, i.e., the actual recovery processes involved. Therefore, quality factors derived from radiation quality alone are inadequate parameters for assessing the radiation risks of space flight. Until recently, OH radicals formed in bulk nuclear water were believed to be the major causes of DNA damage that results in cell death, especially for sparsely ionizing radiations. That hypothesis has now been challenged, if not refuted. Lethal genomic DNA damage is determined mainly by energy deposition in Deoxyribonucleoproteins, and their hydration shells, and charge (energy) transfer processes within those structures.