The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform

Hindritiani Reti - One of the best experts on this subject based on the ideXlab platform.

  • AKTIVITAS DEPIGMENTASI FRAKSI N-HEKSANA BUAH MALAKA (PHYLLANTHUS EMBLICA) PADA SEL MELANOSIT MOUSE MELANOMA B16
    'Universitas Padjadjaran', 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Setiawan Setiawan, Sujatno Muchtan
    Abstract:

    AbstractInhibitor of melanin synthesis can be used as Depigmenting Agent to treat skin hyperpigmentation. Herbal Depigmenting Agents are increasingly utilized due to their character in inhibiting melanin synthesis without causing melanocyte toxicity. This study was aimed to examine Depigmenting activity of n-hexane fraction of Phyllanthus emblica fruit by measuring its melanin synthesisinhibition on melanocyte mouse melanoma B16 cell line. Melanin synthesis inhibition was measured spectrophotometrically by counting the melanin and the activity of tyrosinase. The cytotoxicity of n-hexane fraction on melanocytes was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November-December2009 at Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study indicated that melanin count and tyrosinase activity decreased in a dose-dependent manner of n-hexane fraction of P. emblica fruit (IC50 31.68 and 77.92 μg/ml, respectively). The viable cells decreased with increasing concentration ofthe n-hexane fraction of P. emblica fruit (LD50 87.39 μg/ml).Key words: n-hexane fraction, Phyllanthus emblica, Depigmenting agen

  • Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line
    Faculty of Medicine Universitas Padjadjaran, 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Sujatno Muchtan, Dhianawaty Diah, - Setiawan
    Abstract:

    Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting Agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential Depigmenting Agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.11

- Setiawan - One of the best experts on this subject based on the ideXlab platform.

  • Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line
    Faculty of Medicine Universitas Padjadjaran, 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Sujatno Muchtan, Dhianawaty Diah, - Setiawan
    Abstract:

    Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting Agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential Depigmenting Agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.11

Sujatno Muchtan - One of the best experts on this subject based on the ideXlab platform.

  • AKTIVITAS DEPIGMENTASI FRAKSI N-HEKSANA BUAH MALAKA (PHYLLANTHUS EMBLICA) PADA SEL MELANOSIT MOUSE MELANOMA B16
    'Universitas Padjadjaran', 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Setiawan Setiawan, Sujatno Muchtan
    Abstract:

    AbstractInhibitor of melanin synthesis can be used as Depigmenting Agent to treat skin hyperpigmentation. Herbal Depigmenting Agents are increasingly utilized due to their character in inhibiting melanin synthesis without causing melanocyte toxicity. This study was aimed to examine Depigmenting activity of n-hexane fraction of Phyllanthus emblica fruit by measuring its melanin synthesisinhibition on melanocyte mouse melanoma B16 cell line. Melanin synthesis inhibition was measured spectrophotometrically by counting the melanin and the activity of tyrosinase. The cytotoxicity of n-hexane fraction on melanocytes was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November-December2009 at Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study indicated that melanin count and tyrosinase activity decreased in a dose-dependent manner of n-hexane fraction of P. emblica fruit (IC50 31.68 and 77.92 μg/ml, respectively). The viable cells decreased with increasing concentration ofthe n-hexane fraction of P. emblica fruit (LD50 87.39 μg/ml).Key words: n-hexane fraction, Phyllanthus emblica, Depigmenting agen

  • Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line
    Faculty of Medicine Universitas Padjadjaran, 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Sujatno Muchtan, Dhianawaty Diah, - Setiawan
    Abstract:

    Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting Agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential Depigmenting Agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.11

Rosalie M Luiten - One of the best experts on this subject based on the ideXlab platform.

  • Effective Melanoma Immunotherapy in Mice by the Skin- Depigmenting Agent Monobenzone and the Adjuvants
    2013
    Co-Authors: Jasper G Van Den Boorn, Cornelis J M Melief, Daisy I Picavet, Debby Konijnenberg, Esther P. M. Tjin, Jan D. Bos, Nico J, Rosalie M Luiten
    Abstract:

    Background: Presently melanoma still lacks adequate treatment options for metastatic disease. While melanoma is exceptionally challenging to standard regimens, it is suited for treatment with immunotherapy based on its immunogenicity. Since treatment-related skin depigmentation is considered a favourable prognostic sign during melanoma intervention, we here aimed at the reverse approach of directly inducing vitiligo as a shortcut to effective anti-melanoma immunity. Methodology and Principal Findings: We developed an effective and simple to use form of immunotherapy by combining the topical skin-bleaching Agent monobenzone with immune-stimulatory imiquimod cream and cytosine-guanine oligodeoxynucleotides (CpG) injections (MIC therapy). This powerful new approach promptly induced a melanoma antigenspecific immune response, which abolished subcutaneous B16.F10 melanoma growth in up to 85 % of C57BL/6 mice. Importantly, this regimen induced over 100 days of tumor-free survival in up to 60 % of the mice, and forcefully suppressed tumor growth upon re-challenge either 65- or 165 days after MIC treatment cessation. Conclusions: MIC therapy is effective in eradicating melanoma, by vigilantly incorporating NK-, B- and T cells in its therapeutic effect. Based on these results, the MIC regimen presents a high-yield, low-cost and simple therapy, readil

  • monobenzone induced depigmentation from enzymatic blockade to autoimmunity
    Pigment Cell & Melanoma Research, 2011
    Co-Authors: Jasper G Van Den Boorn, Cornelis J M Melief, Rosalie M Luiten
    Abstract:

    Summary Autoimmune side-effects such as vitiligo regularly occur during melanoma immunotherapy. As vitiligo development is associated with a superior prognosis, the active induction of vitiligo in melanoma patients can be a useful tactic. The potent skin-Depigmenting Agent monobenzone can be used successfully for this purpose. However, until recently, the mechanism of action behind monobenzone-induced skin depigmentation was unclear. Lately, the mechanistic basis for the augmented immunogenicity of monobenzone-exposed pigmented cells has been unveiled, and their active role in the induction of autoimmune T-cell-mediated vitiligo has become apparent. Here, we provide an immunological framework in which we condense this knowledge to an integrated theory of the generation of monobenzone-induced vitiligo.

  • effective melanoma immunotherapy in mice by the skin Depigmenting Agent monobenzone and the adjuvants imiquimod and cpg
    PLOS ONE, 2010
    Co-Authors: Jasper G Van Den Boorn, Cornelis J M Melief, Daisy I Picavet, Debby Konijnenberg, Esther P. M. Tjin, Nico J. Meeuwenoord, Dmitri V. Filippov, J Wietze P Van Der Veen, Rosalie M Luiten
    Abstract:

    Background: Presently melanoma still lacks adequate treatment options for metastatic disease. While melanoma is exceptionally challenging to standard regimens, it is suited for treatment with immunotherapy based on its immunogenicity. Since treatment-related skin depigmentation is considered a favourable prognostic sign during melanoma intervention, we here aimed at the reverse approach of directly inducing vitiligo as a shortcut to effective anti-melanoma immunity. Methodology and Principal Findings: We developed an effective and simple to use form of immunotherapy by combining the topical skin-bleaching Agent monobenzone with immune-stimulatory imiquimod cream and cytosine-guanine oligodeoxynucleotides (CpG) injections (MIC therapy). This powerful new approach promptly induced a melanoma antigen-specific immune response, which abolished subcutaneous B16. F10 melanoma growth in up to 85% of C57BL/6 mice. Importantly, this regimen induced over 100 days of tumor-free survival in up to 60% of the mice, and forcefully suppressed tumor growth upon re-challenge either 65- or 165 days after MIC treatment cessation. Conclusions: MIC therapy is effective in eradicating melanoma, by vigilantly incorporating NK-, B-and T cells in its therapeutic effect. Based on these results, the MIC regimen presents a high-yield, low-cost and simple therapy, readily applicable in the clinic

Sutedja Endang - One of the best experts on this subject based on the ideXlab platform.

  • AKTIVITAS DEPIGMENTASI FRAKSI N-HEKSANA BUAH MALAKA (PHYLLANTHUS EMBLICA) PADA SEL MELANOSIT MOUSE MELANOMA B16
    'Universitas Padjadjaran', 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Setiawan Setiawan, Sujatno Muchtan
    Abstract:

    AbstractInhibitor of melanin synthesis can be used as Depigmenting Agent to treat skin hyperpigmentation. Herbal Depigmenting Agents are increasingly utilized due to their character in inhibiting melanin synthesis without causing melanocyte toxicity. This study was aimed to examine Depigmenting activity of n-hexane fraction of Phyllanthus emblica fruit by measuring its melanin synthesisinhibition on melanocyte mouse melanoma B16 cell line. Melanin synthesis inhibition was measured spectrophotometrically by counting the melanin and the activity of tyrosinase. The cytotoxicity of n-hexane fraction on melanocytes was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November-December2009 at Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study indicated that melanin count and tyrosinase activity decreased in a dose-dependent manner of n-hexane fraction of P. emblica fruit (IC50 31.68 and 77.92 μg/ml, respectively). The viable cells decreased with increasing concentration ofthe n-hexane fraction of P. emblica fruit (LD50 87.39 μg/ml).Key words: n-hexane fraction, Phyllanthus emblica, Depigmenting agen

  • Penurunan Aktivitas Tirosinase dan Jumlah Melanin oleh Fraksi Etil Asetat Buah Malaka (Phyllantus emblica) pada Mouse Melanoma B16 Cell-Line
    Faculty of Medicine Universitas Padjadjaran, 2013
    Co-Authors: Hindritiani Reti, Sutedja Endang, Sujatno Muchtan, Dhianawaty Diah, - Setiawan
    Abstract:

    Akumulasi melanin menyebabkan hiperpigmentasi dan bila terdapat pada wajah dapat menimbulkan masalah psikososial. Bahan depigmentasi dari tanaman semakin banyak digunakan. Bahan yang dikembangkan harus efektif menghambat sintesis melanin serta tidak toksik terhadap melanosit. Penelitian ini bertujuan menguji efek fraksi etil asetat buah Phyllanthus emblica (P. emblica) atau buah malaka, terhadap sintesis melanin dengan mengukur jumlah melanin dan aktivitas tirosinase, yang merupakan enzim utama sintesis melanin, secara spektrofotometrik pada kultur sel melanosit mouse melanoma B16 cell-line. Sitotoksisitas diukur dengan metode 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Penelitian dilakukan pada November–Desember 2009 di Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, Korea Selatan. Hasil penelitian menunjukkan aktivitas tirosinase dan jumlah melanin menurun bergantung pada dosis perlakuan berbagai konsentrasi fraksi etil asetat buah P. emblica dengan inhibition concentration (IC) 50 berturutturut 95,63 dan 16,90 μg/mL, serta lethal dose (LD) 50 pada konsentrasi 106,64 μg/mL. Simpulan, fraksi etil asetat buah P. emblica mempunyai potensi sebagai bahan depigmentasi, karena mampu menurunkan sintesis melanin melalui inhibisi aktivitas tirosinase. [MKB. 2013;45(2):118–24]Kata kunci: Aktivitas tirosinase, etil asetat, melanin, Phyllanthus emblicaReduction of Tyrosinase Activity and Melanin Amount by Ethyl Acetate Fraction from Malaka (Phyllanthus emblica) Fruit in Mouse Melanoma B16 Cell-LineMelanin accumulation can lead to hyperpigmentation, and if it occurs on the face can cause psychosocial problem. Depigmenting Agents derived from plants are increasingly utilized. Agents being developed have to be effective in inhibiting melanin synthesis and should not be toxic to melanocyte. This study aimed was to examine the effect of ethyl acetate fraction from Phyllanthus emblica (P. emblica) fruit, also known as malaka fruit, towards melanine synthesis, which was measured from the melanin amount and tyrosinase activity, the key regulatory enzyme in melanin synthesis, spectrophotometrically towards melanocytes of mouse melanoma B16 cell-line. The cytotoxic effect towards melanocytes was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November−December 2009 in Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study showed that tyrosinase activity and melanin amount decreased in a dose-dependent manner towards various concentrations of ethyl acetate fraction of P. emblica fruit with inhibition concentration (IC) 50=95.63 and 16.90 μg/mL, respectively and lethal dose (LD) 50 concentration 106.64 μg/mL. In conclusion, ethyl acetate fraction of P. emblica fruit is a potential Depigmenting Agent, since it can reduce melanin synthesis by inhibition of tyrosinase activity. [MKB. 2013;45(2):119–24]Key words: Ethyl acetate, melanin, Phyllanthus emblica, tyrosinase activity DOI: http://dx.doi.org/10.15395/mkb.v45n2.11