The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Masataka Shiozuka - One of the best experts on this subject based on the ideXlab platform.
-
Transdermal Delivery of Adriamycin to Transplanted Ehrlich Ascites Tumor in Mice
Pharmaceutics, 2013Co-Authors: Masataka Shiozuka, Yoshiaki Nonomura, Ryoichi MatsudaAbstract:There is considerable interest in the skin as a site of anti-cancer drug application. Nevertheless, the skin poses a formidable barrier to drug penetration, thereby limiting topical and transdermal bioavailability. However, we previously showed that a thioglycolate-based Depilatory Agent increases the drug permeability of mouse skin. In the present report, we investigated the skin permeability and efficacy of the anti-cancer drug adriamycin increased when administered transdermally to mice in combination with a thioglycolate-based Depilatory Agent. Adriamycin in combination with Depilatory treatment reduced Ehrlich tumor growth in hairless mice about the weight and size of harvested tumors. In addition, our delivery method for adriamycin increased the therapeutic effectiveness of this Agent by decreasing toxicity. Moreover, measurement of adriamycin autofluorescence revealed that topically applied adriamycin penetrate the dermis after Depilatory Agent treatment. These results indicate that the transdermal delivery of anti-cancer drugs is feasible by handy pretreatment of the skin with a thioglycolate-based Depilatory Agent.
-
Therapeutic readthrough strategy for suppression of nonsense mutations in duchenne muscular dystrophy
Brain and nerve, 2011Co-Authors: Masataka Shiozuka, Riyoichi MatsudaAbstract:: Effective treatment for Duchenne muscular dystrophy (DMD) is currently unavailable. Readthrough of disease-causing premature termination codons might alleviate the symptoms of genetic diseases caused by nonsense mutations. Several ribosome-binding compounds, including selective antibiotics and synthetic novel small molecules, induce translational readthrough, restoring full-length functional proteins. Here in this innovative therapeutic strategy has been summarized with a focus on DMD. We have previously reported that negamycin restored dystrophin expression with less toxicity than gentamicin in mdx mice. To explore more potent readthrough inducers, we established the transgenic mouse called READ (readthrough evaluation and assessment by dural receptor) for readthrough-specific detection. Using READ mice, we discovered drug candidates, including sterically negamycin-like small molecules and aminoglycoside derivatives. The newly developed small molecules induced dose-dependent readthrough with greater potency than ataluren in vitro and promoted the expression of dystrophin and reduction in serum creatine kinase activity in mdx mice. Moreover, the aminoglycoside derivative restored both dystrophin protein and contractile function of mdx skeletal muscles with appreciably higher readthrough activity and lower toxicity than that of gentamicin. Furthermore, we confirmed the efficacy of a thioglycolate-based Depilatory Agent to enhance the topical delivery of skin-impermeable drugs, including aminoglycosides. These promising new chemotherapeutic Agents with beneficial effects on readthrough action, lower toxicity, and transdermal delivery may have significant value in treating or preventing genetic diseases caused by nonsense mutations.
-
Transdermal delivery of a readthrough-inducing drug: a new approach of gentamicin administration for the treatment of nonsense mutation-mediated disorders
Journal of Biochemistry, 2009Co-Authors: Masataka Shiozuka, Yoshiaki Nonomura, Akira Wagatsuma, Tadafumi Kawamoto, Hiroyuki Sasaki, Kenichi Shimada, Yoshikazu Takahashi, Ryoichi MatsudaAbstract:To induce the readthrough of premature termination codons, aminoglycoside antibiotics such as gentamicin have attracted interest as potential therapeutic Agents for diseases caused by nonsense mutations. The transdermal delivery of gentamicin is considered unfeasible because of its low permeability through the dermis. However, if the skin permeability of gentamicin could be improved, it would allow topical application without the need for systemic delivery. In this report, we demonstrated that the skin permeability of gentamicin increased with the use of a thioglycolate-based Depilatory Agent. After transdermal administration, the readthrough activity in skeletal muscle, as determined using a lacZ/luc reporter system, was found to be equivalent to systemic administration when measured in transgenic mice. Transdermally applied gentamicin was detected by liquid chromatography-tandem mass spectrometry in the muscles and sera of mice only after Depilatory Agent-treatment. In addition, expansion of the intercellular gaps in the basal and prickle-cell layers was observed by electron microscopy only in the Depilatory Agent-treated mice. Depilatory Agent-treatment may be useful for the topical delivery of readthough-inducing drugs for the rescue of nonsense mutation-mediated genetic disorders. This finding may also be applicable for the transdermal delivery of other pharmacologically active molecules.
Ryoichi Matsuda - One of the best experts on this subject based on the ideXlab platform.
-
Transdermal Delivery of Adriamycin to Transplanted Ehrlich Ascites Tumor in Mice
Pharmaceutics, 2013Co-Authors: Masataka Shiozuka, Yoshiaki Nonomura, Ryoichi MatsudaAbstract:There is considerable interest in the skin as a site of anti-cancer drug application. Nevertheless, the skin poses a formidable barrier to drug penetration, thereby limiting topical and transdermal bioavailability. However, we previously showed that a thioglycolate-based Depilatory Agent increases the drug permeability of mouse skin. In the present report, we investigated the skin permeability and efficacy of the anti-cancer drug adriamycin increased when administered transdermally to mice in combination with a thioglycolate-based Depilatory Agent. Adriamycin in combination with Depilatory treatment reduced Ehrlich tumor growth in hairless mice about the weight and size of harvested tumors. In addition, our delivery method for adriamycin increased the therapeutic effectiveness of this Agent by decreasing toxicity. Moreover, measurement of adriamycin autofluorescence revealed that topically applied adriamycin penetrate the dermis after Depilatory Agent treatment. These results indicate that the transdermal delivery of anti-cancer drugs is feasible by handy pretreatment of the skin with a thioglycolate-based Depilatory Agent.
-
Transdermal delivery of a readthrough-inducing drug: a new approach of gentamicin administration for the treatment of nonsense mutation-mediated disorders
Journal of Biochemistry, 2009Co-Authors: Masataka Shiozuka, Yoshiaki Nonomura, Akira Wagatsuma, Tadafumi Kawamoto, Hiroyuki Sasaki, Kenichi Shimada, Yoshikazu Takahashi, Ryoichi MatsudaAbstract:To induce the readthrough of premature termination codons, aminoglycoside antibiotics such as gentamicin have attracted interest as potential therapeutic Agents for diseases caused by nonsense mutations. The transdermal delivery of gentamicin is considered unfeasible because of its low permeability through the dermis. However, if the skin permeability of gentamicin could be improved, it would allow topical application without the need for systemic delivery. In this report, we demonstrated that the skin permeability of gentamicin increased with the use of a thioglycolate-based Depilatory Agent. After transdermal administration, the readthrough activity in skeletal muscle, as determined using a lacZ/luc reporter system, was found to be equivalent to systemic administration when measured in transgenic mice. Transdermally applied gentamicin was detected by liquid chromatography-tandem mass spectrometry in the muscles and sera of mice only after Depilatory Agent-treatment. In addition, expansion of the intercellular gaps in the basal and prickle-cell layers was observed by electron microscopy only in the Depilatory Agent-treated mice. Depilatory Agent-treatment may be useful for the topical delivery of readthough-inducing drugs for the rescue of nonsense mutation-mediated genetic disorders. This finding may also be applicable for the transdermal delivery of other pharmacologically active molecules.
Robert W Hickey - One of the best experts on this subject based on the ideXlab platform.
-
effect of a Depilatory Agent on cotton polyester and rayon versus human hair in a laboratory setting
Annals of Emergency Medicine, 2015Co-Authors: Jocelyn A Plesa, Kelly Shoup, Mioara D Manole, Robert W HickeyAbstract:Study objective We examine the ability of a Depilatory Agent, Nair, to dissolve strands of hair, cotton, polyester, and rayon. Methods We conducted a bench laboratory study in which we tested single strands of hair and natural and synthetic fibers under static tension with a 10.8-g weight and application of Nair. The dependent variable, time until breakage, was recorded. If the strand did not break within 8 hours, the experiment was discontinued. Three types of hair were tested (thin, medium, and thick, as recorded per diameter). Three types of natural and synthetic fibers were tested (cotton, polyester, and rayon). Results All types of hair had breakage within 10 minutes of the Nair application. Synthetic materials had no breakage after 8 hours with application of Nair. Conclusion Depilatory Agents dissolve hair under tension within minutes. However, they do not dissolve cotton, polyester, and rayon even after many hours of application.
Lisa A. Delouise - One of the best experts on this subject based on the ideXlab platform.
-
Quantification of human skin barrier function and susceptibility to quantum dot skin penetration
Nanotoxicology, 2011Co-Authors: Supriya Ravichandran, Luke J. Mortensen, Lisa A. DelouiseAbstract:Abstract In this study, we utilize in vivo human skin and a viable ex-vivo human skin model to investigate the effect of a commercial Depilatory Agent on barrier function. Tape stripping was used as a positive control. The magnitude of skin barrier was quantified by measuring transepidermal water loss values on in vivo human skin and transepithelial electrical resistance measurements and tissue histology on ex vivo skin. The susceptibility to carboxylated quantum dot penetration through ex vivo skin was investigated using fluorescent microcopy analysis of microtomed skin sections and flow cytometry to quantify quantum dot association with live epidermal cells. Results show that Depilatory treatment modifies the outside-in barrier sufficiently to allow quantum dots to penetrate the stratum corneum but to a lesser extent than tape stripping. The implications of these finding are discussed.
Jocelyn A Plesa - One of the best experts on this subject based on the ideXlab platform.
-
effect of a Depilatory Agent on cotton polyester and rayon versus human hair in a laboratory setting
Annals of Emergency Medicine, 2015Co-Authors: Jocelyn A Plesa, Kelly Shoup, Mioara D Manole, Robert W HickeyAbstract:Study objective We examine the ability of a Depilatory Agent, Nair, to dissolve strands of hair, cotton, polyester, and rayon. Methods We conducted a bench laboratory study in which we tested single strands of hair and natural and synthetic fibers under static tension with a 10.8-g weight and application of Nair. The dependent variable, time until breakage, was recorded. If the strand did not break within 8 hours, the experiment was discontinued. Three types of hair were tested (thin, medium, and thick, as recorded per diameter). Three types of natural and synthetic fibers were tested (cotton, polyester, and rayon). Results All types of hair had breakage within 10 minutes of the Nair application. Synthetic materials had no breakage after 8 hours with application of Nair. Conclusion Depilatory Agents dissolve hair under tension within minutes. However, they do not dissolve cotton, polyester, and rayon even after many hours of application.