The Experts below are selected from a list of 110610 Experts worldwide ranked by ideXlab platform
G Jundt - One of the best experts on this subject based on the ideXlab platform.
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in situ measurement of collagen synthesis by human bone cells with a sirius red based colorimetric microassay effects of transforming growth factor β2 and ascorbic acid 2 phosphate
Histochemistry and Cell Biology, 1999Co-Authors: H Tullbergreinert, G JundtAbstract:Staining of collagens by Sirius Red, a standard histological procedure, was applied to quantify collagen synthesis in human osteoblast-like cell cultures in situ. After morphological analysis of the Deposited Material, the stain was dissolved and its optical density determined spectrophotometrically using a microtiter plate assay system. The method was sensitive with a detection limit for collagen synthesized by 3000 normal human periosteal cells. The assay is easy to perform and specific with respect to different extracellular Materials, for example, collagen types I and III were well stained, collagen type IV and laminin exhibited only low staining, and fibronectin, chondroitin sulfate, dermatan sulfate, and amyloid β were negative. A major advantage of the method is the combination of identification of collagen-producing cells in situ with subsequent spectrophotometric quantification of the dissolved stain. Thus it is possible to obtain information on cell morphology, active sites of collagen deposition in a cell culture, microscopic detection of high-and low-producer cells prior to dissolution and quantification of the Deposited Material. In this regard the assay is superior to either radioactive labeling, hydroxyproline determination, or Sirius Red-based colorimetric assays with cell lysates. Since the quantification is based on microtiter plate reading, the method can be recommended for the screening of large quantities of samples.
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in situ measurement of collagen synthesis by human bone cells with a sirius red based colorimetric microassay effects of transforming growth factor beta2 and ascorbic acid 2 phosphate
Histochemistry and Cell Biology, 1999Co-Authors: H Tullbergreinert, G JundtAbstract:Staining of collagens by Sirius Red, a standard histological procedure, was applied to quantify collagen synthesis in human osteoblast-like cell cultures in situ. After morphological analysis of the Deposited Material, the stain was dissolved and its optical density determined spectrophotometrically using a microtiter plate assay system. The method was sensitive with a detection limit for collagen synthesized by 3000 normal human periosteal cells. The assay is easy to perform and specific with respect to different extracellular Materials, for example, collagen types I and III were well stained, collagen type IV and laminin exhibited only low staining, and fibronectin, chondroitin sulfate, dermatan sulfate, and amyloid beta were negative. A major advantage of the method is the combination of identification of collagen-producing cells in situ with subsequent spectrophotometric quantification of the dissolved stain. Thus it is possible to obtain information on cell morphology, active sites of collagen deposition in a cell culture, microscopic detection of high-and low-producer cells prior to dissolution and quantification of the Deposited Material. In this regard the assay is superior to either radioactive labeling, hydroxyproline determination, or Sirius Red-based colorimetric assays with cell lysates. Since the quantification is based on microtiter plate reading, the method can be recommended for the screening of large quantities of samples.
H Tullbergreinert - One of the best experts on this subject based on the ideXlab platform.
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in situ measurement of collagen synthesis by human bone cells with a sirius red based colorimetric microassay effects of transforming growth factor β2 and ascorbic acid 2 phosphate
Histochemistry and Cell Biology, 1999Co-Authors: H Tullbergreinert, G JundtAbstract:Staining of collagens by Sirius Red, a standard histological procedure, was applied to quantify collagen synthesis in human osteoblast-like cell cultures in situ. After morphological analysis of the Deposited Material, the stain was dissolved and its optical density determined spectrophotometrically using a microtiter plate assay system. The method was sensitive with a detection limit for collagen synthesized by 3000 normal human periosteal cells. The assay is easy to perform and specific with respect to different extracellular Materials, for example, collagen types I and III were well stained, collagen type IV and laminin exhibited only low staining, and fibronectin, chondroitin sulfate, dermatan sulfate, and amyloid β were negative. A major advantage of the method is the combination of identification of collagen-producing cells in situ with subsequent spectrophotometric quantification of the dissolved stain. Thus it is possible to obtain information on cell morphology, active sites of collagen deposition in a cell culture, microscopic detection of high-and low-producer cells prior to dissolution and quantification of the Deposited Material. In this regard the assay is superior to either radioactive labeling, hydroxyproline determination, or Sirius Red-based colorimetric assays with cell lysates. Since the quantification is based on microtiter plate reading, the method can be recommended for the screening of large quantities of samples.
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in situ measurement of collagen synthesis by human bone cells with a sirius red based colorimetric microassay effects of transforming growth factor beta2 and ascorbic acid 2 phosphate
Histochemistry and Cell Biology, 1999Co-Authors: H Tullbergreinert, G JundtAbstract:Staining of collagens by Sirius Red, a standard histological procedure, was applied to quantify collagen synthesis in human osteoblast-like cell cultures in situ. After morphological analysis of the Deposited Material, the stain was dissolved and its optical density determined spectrophotometrically using a microtiter plate assay system. The method was sensitive with a detection limit for collagen synthesized by 3000 normal human periosteal cells. The assay is easy to perform and specific with respect to different extracellular Materials, for example, collagen types I and III were well stained, collagen type IV and laminin exhibited only low staining, and fibronectin, chondroitin sulfate, dermatan sulfate, and amyloid beta were negative. A major advantage of the method is the combination of identification of collagen-producing cells in situ with subsequent spectrophotometric quantification of the dissolved stain. Thus it is possible to obtain information on cell morphology, active sites of collagen deposition in a cell culture, microscopic detection of high-and low-producer cells prior to dissolution and quantification of the Deposited Material. In this regard the assay is superior to either radioactive labeling, hydroxyproline determination, or Sirius Red-based colorimetric assays with cell lysates. Since the quantification is based on microtiter plate reading, the method can be recommended for the screening of large quantities of samples.
Gerd Leuchs - One of the best experts on this subject based on the ideXlab platform.
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unveiling the optical properties of a metaMaterial synthesized by electron beam induced deposition
Nanotechnology, 2016Co-Authors: Pawel Woźniak, Katja Hoflich, Gerald Bronstrup, Peter Banzer, Silke Christiansen, Gerd LeuchsAbstract:Direct writing using a focused electron beam allows for fabricating truly three-dimensional structures of sub-wavelength dimensions in the visible spectral regime. The resulting sophisticated geometries are perfectly suited for studying light-matter interaction at the nanoscale. Their overall optical response will strongly depend not only on geometry but also on the optical properties of the Deposited Material. In the case of the typically used metal-organic precursors, the deposits show a substructure of metallic nanocrystals embedded in a carbonaceous matrix. Since gold-containing precursor media are especially interesting for optical applications, we experimentally determine the effective permittivity of such an effective Material. Our experiment is based on spectroscopic measurements of planar deposits. The retrieved permittivity shows a systematic dependence on the gold particle density and cannot be sufficiently described using the common Maxwell-Garnett approach for effective medium.
Marlon Wesley Machado Cunico - One of the best experts on this subject based on the ideXlab platform.
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development of new rapid prototyping process
Rapid Prototyping Journal, 2011Co-Authors: Marlon Wesley Machado Cunico, Carlos Marcus Gomes Da Silva Cruz, Neri VolpatoAbstract:Purpose – The purpose of this paper is to identify the key elements of a new rapid prototyping process, which involves layer‐by‐layer deposition of liquid‐state Material and at the same time using an ultraviolet line source to cure the Deposited Material. This paper reports studies about the behaviour of filaments, deposition accuracy, filaments interaction and functional feasibility of system. Additionally, the author describes the process which has been proposed, the equipment that has been used for these studies and the Material which was developed in this application.Design/methodology/approach – The research has been separated into three study areas in accordance with their goals. In the first, both the behaviour of filament and deposition accuracy was studied. The design of the experiment is described with focus on four response factors (bead width, filament quality, deposition accuracy and deposition continuity) along with function of three control factors (deposition height, deposition velocity an...
Yiming Rong - One of the best experts on this subject based on the ideXlab platform.
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competitive failure analysis on tensile fracture of laser Deposited Material for martensitic stainless steel
Materials & Design, 2017Co-Authors: Gang Wang, Jianchao Yu, Yiming RongAbstract:Abstract Sufficient mechanical properties of Deposited Materials are needed in remanufacturing applications to guarantee the functionality and reliability of repaired parts. The present paper described the tensile fracture behavior of laser-Deposited FV520B martensitic stainless steel comprehensively based on evolution of the microstructure and mechanical property. The samples were fabricated by laser hot-wire deposition. The fracture behavior of the Deposited samples was characterized using a specially designed uniaxial tension testing approach and investigated on the grain size, microhardness profile, residual stresses and precipitate distribution. The results show that the tensile fracture occurred at the position with high fluctuating microhardness caused by the multi-layer laser heating. Three tensile fracture patterns of Deposited Material were found: interfacial fracture, heat affected zone (HAZ) fracture and HAZ/interface hybrid fracture. They result from the competitive failure between microvoid coalescence in the heat affected zone and interfacial cracking in the clad layer/HAZ interface. Grain refinement and dissolution of precipitates occurred in heat affected zone, leading to a decrease of strength and increase of toughness.