The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform

Ole Bang - One of the best experts on this subject based on the ideXlab platform.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    Biomedical Optics Express, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR-OCT image has previously been reported, despite many publications within this field. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20 μm, and in imaging the Dermal Papillae where, when utilising the UHR-OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    arXiv: Medical Physics, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised by the lower surface of a single cell layer in epidermis connected to an even thinner basement membrane, attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20mm, and in imaging the Dermal Papillae where capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.

Niels Moller Israelsen - One of the best experts on this subject based on the ideXlab platform.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    Biomedical Optics Express, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR-OCT image has previously been reported, despite many publications within this field. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20 μm, and in imaging the Dermal Papillae where, when utilising the UHR-OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    arXiv: Medical Physics, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised by the lower surface of a single cell layer in epidermis connected to an even thinner basement membrane, attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20mm, and in imaging the Dermal Papillae where capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.

Colin A B Jahoda - One of the best experts on this subject based on the ideXlab platform.

  • Dynamic expression of the zinc-finger transcription factor Trps1 during hair follicle morphogenesis and cycling
    Gene Expression Patterns, 2007
    Co-Authors: Katherine A. Fantauzzo, Colin A B Jahoda, Hisham Bazzi, Angela M Christiano
    Abstract:

    Mutations in the gene encoding the zinc finger transcription factor TRPS1 result in tricho-rhino-phalangeal syndrome, characterized by craniofacial and skeletal abnormalities, and sparse scalp hair. In this study, Trps1 was identified by microarray hybridization analysis as having a complex pattern of spatiotemporal regulation in murine skin during morphogenesis. During early skin development, Trps1 expression decreased in the epidermis while simultaneously increasing in the dermis. Trps1 was specifically expressed in the nuclei of mesenchymal cells during hair follicle morphogenesis. An analysis of Trps1 expression during postnatal murine hair follicle cycling revealed that the protein localized to the nuclei of Dermal Papillae cells during telogen and anagen. Additionally, we found that Trps1 consistently localized to the nuclei of Dermal Papillae cells and the highly proliferative epithelial cells of mouse, rat and human hair follicles.

  • trans species hair growth induction by human hair follicle Dermal Papillae
    Experimental Dermatology, 2001
    Co-Authors: Colin A B Jahoda, R F Oliver, Amanda J Reynolds, J C Forrester, J W Gillespie, P B Cserhalmifriedman, Angela M Christiano, K A Horne
    Abstract:

    : A series of experimental bioassays has shown that the Dermal papilla of the adult rodent vibrissa hair follicle retains unique inductive properties. In view of the many phenotypic and functional differences between specific hair follicle types, and the growing interest in hair follicle biology and disease, it remains important to establish that the human hair follicle Dermal papilla has equivalent capabilities. In this study we tested the ability of human hair follicle Papillae to induce hair growth when implanted into transected, athymic mouse vibrissa follicles. The implanted Papillae that interacted with mouse follicle epithelium created new fibre-producing follicle end bulbs. The origin of the Papillae in the recombinant structures was confirmed using laser capture microdissection and human specific gender determination by PCR. The demonstration that intact adult human Dermal Papillae can induce hair growth has implications for molecular analysis of basic hair growth mechanisms, particularly since the study involved common epithelial-mesenchymal signalling and recognition properties across species. It also improves the prospects for a cell-based clinical approach to hair follicle disorders.

  • induction of follicle formation and hair growth by vibrissa Dermal Papillae implanted into rat ear wounds vibrissa type fibres are specified
    Development, 1992
    Co-Authors: Colin A B Jahoda
    Abstract:

    Adult vibrissa follicle Dermal Papillae have the capacity to induce hair growth and follicle formation when associated with epidermis from various sources. However, the range of conditions under which hair follicle induction will take place has not been established. The question of whether or not the adult papilla carries information to impose fibre-type specificity has also not been fully answered. This study describes how the implantation of isolated Papillae into small incisional cuts on the rat ear pinna resulted in the subsequent emergence of abnormally large hair fibres from the wound sites. Many of these hairs were found to display vibrissa-type characteristics. Histological observations indicated that the Papillae had interacted with the edges of the wound epidermis to produce new, and particularly large follicles, while immunohistochemical staining revealed that early follicle construction was accompanied by a profusion of the basement membrane constituents laminin and type IV collagen in the subjacent dermis. These findings show that adult rat Papillae retain the capacity, as displayed by embryonic dermis, to determine vibrissa specificity in induced follicles.

G P M Moore - One of the best experts on this subject based on the ideXlab platform.

  • sheep vibrissa Dermal Papillae induce hair follicle formation in heterotypic skin equivalents
    British Journal of Dermatology, 1994
    Co-Authors: S A J Watson, P Pisansarakit, G P M Moore
    Abstract:

    Summary Cultured skin equivalents were constructed by combining keratinocytes, outer root sheath cells or isolated epidermis, in vitro, with a matrix composed of collagen and cultured fibroblasts. When equivalents were grafted on to host animals, the epidermis thickened considerably, and tongues of cells penetrated the dermis, giving the Dermal/epiDermal junction a deeply sculptured profile. No cutaneous appendages were found in these grafts. We explored the possibility of inducing hair follicles by incorporating ovine hair follicle Dermal Papillae into constructs composed of an isolated epiDermal sheet and a contracted Dermal equivalent. In vitro, no morphogenetic changes associated with follicle formation were observed in the recombinants, but when grafted on to nude mice, follicle-like structures were identified. The follicles were large, and had developed adjacent to the epidermis, indicating that the matrix environment of the induced follicles may not have been compatible with the downgrowth of the epiDermal plugs normally observed during follicle formation in living skin. Nevertheless, in histological sections, the induced structures displayed many of the morphological characteristics of follicles in vivo, including the production of keratinized hairs. These results indicate that skin equivalents provide a useful model for the study of the chemical and structural features of matrices that facilitate hair follicle development.

Merete Haedersdal - One of the best experts on this subject based on the ideXlab platform.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    Biomedical Optics Express, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised of the lower surface of a single cell layer in epidermis (the stratum basale) connected to an even thinner membrane (the basement membrane). The basement membrane is attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system (Vivosight from Michaelson Diagnostics) and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. To our knowledge no detailed delineating of the DEJ in the UHR-OCT image has previously been reported, despite many publications within this field. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20 μm, and in imaging the Dermal Papillae where, when utilising the UHR-OCT, capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.

  • the value of ultrahigh resolution oct in dermatology delineating the dermo epiDermal junction capillaries in the Dermal Papillae and vellus hairs
    arXiv: Medical Physics, 2018
    Co-Authors: Niels Moller Israelsen, Michael Maria, Mette Mogensen, Sophie Bojesen, Mikkel Jensen, Merete Haedersdal, Adrian Gh Podoleanu, Ole Bang
    Abstract:

    Optical coherence tomography (OCT) imaging of the skin is gaining recognition and is increasingly applied to dermatological research. A key dermatological parameter inferred from an OCT image is the epiDermal (Ep) thickness as a thickened Ep can be an indicator of a skin disease. Agreement in the literature on the signal characters of Ep and the subjacent skin layer, the dermis (D), is evident. Ambiguities of the OCT signal interpretation in the literature is however seen for the transition region between the Ep and D, which from histology is known as the dermo-epiDermal junction (DEJ); a distinct junction comprised by the lower surface of a single cell layer in epidermis connected to an even thinner basement membrane, attached to the underlying dermis. In this work we investigate the impact of an improved axial and lateral resolution on the applicability of OCT for imaging of the skin. To this goal, OCT images are compared produced by a commercial OCT system and by an in-house built ultrahigh resolution (UHR-) OCT system for dermatology. In 11 healthy volunteers, we investigate the DEJ signal characteristics. We perform a detailed analysis of the dark (low) signal band clearly seen for UHR-OCT in the DEJ region where we, by using a transition function, find the signal transition of axial sub-resolution character, which can be directly attributed to the exact location of DEJ, both in normal (thin/hairy) and glabrous (thick) skin. For selected healthy volunteers, we investigate the Dermal Papillae and the vellus hairs and identify distinct features that only UHR-OCT can resolve. Differences are seen in tracing hairs of diameter below 20mm, and in imaging the Dermal Papillae where capillary structures are identified in the hand palm, not previously reported in OCT studies and specifically for glabrous skin not reported in any other in vivo optical imaging studies.