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Masahiro Nakano - One of the best experts on this subject based on the ideXlab platform.

  • Dermal Patch anaesthesia comparison of 10 lignocaine gel with absorption promoter and emla cream
    Anaesthesia, 1993
    Co-Authors: Masakatsu Sakamoto, Tatsuhiko Kano, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Osamu Shimoda, Masahiro Nakano
    Abstract:

    The analgesic effects of transDermally applied 10% lignocaine aquagel containing 3% glycyrrhetinic acid monohemiphthalate disodium (as an absorption enhancer) and EMLA cream were compared on the forearms of 34 adult volunteers in a double-blind fashion. The mean pinprick pain scores (graded by noting the number of painful pinpricks out of five) at 30, 60 and 90 min after application and 30 min after removal of the anaesthetics were 3.3 (0.3) (mean SE), 1.2 (0.3), 0.3 (0.1) and 0.3 (0.1) respectively, in the lignocaine gel group. Corresponding scores were 3.5 (0.3), 1.5 (0.3), 0.7 (0.2) and 0.1 (0.1) respectively, in the EMLA group. Insertion of a 26-gauge needle into the treated skin to a depth of 1 mm at 90 min after application was not painful in 91% of the volunteers in the lignocaine gel group and 88% of those in the EMLA group. There was no significant difference in any of the corresponding pain scores between the two groups.

  • Dermal Patch anaesthesia: comparison of 10% lignocaine gel with absorption promoter and EMLA cream
    Anaesthesia, 1993
    Co-Authors: Masakatsu Sakamoto, Tatsuhiko Kano, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Osamu Shimoda, Masahiro Nakano
    Abstract:

    The analgesic effects of transDermally applied 10% lignocaine aquagel containing 3% glycyrrhetinic acid monohemiphthalate disodium (as an absorption enhancer) and EMLA cream were compared on the forearms of 34 adult volunteers in a double-blind fashion. The mean pinprick pain scores (graded by noting the number of painful pinpricks out of five) at 30, 60 and 90 min after application and 30 min after removal of the anaesthetics were 3.3 (0.3) (mean SE), 1.2 (0.3), 0.3 (0.1) and 0.3 (0.1) respectively, in the lignocaine gel group. Corresponding scores were 3.5 (0.3), 1.5 (0.3), 0.7 (0.2) and 0.1 (0.1) respectively, in the EMLA group. Insertion of a 26-gauge needle into the treated skin to a depth of 1 mm at 90 min after application was not painful in 91% of the volunteers in the lignocaine gel group and 88% of those in the EMLA group. There was no significant difference in any of the corresponding pain scores between the two groups.

  • skin pretreatments for shortening onset of Dermal Patch anesthesia with 3 ga mhph 2na 10 lidocaine gel mixture
    Anesthesia & Analgesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Three types of skin pretreatments (stripping with adhesive tape, scrubbing with Skin-Pure jelly, and cleaning with benzine) were studied in 21 volunteers to determine whether the pretreatments could shorten the application period of transDermal 3% GA MHPh 2Na-10% lidocaine gel. Before application of the gel, each subject received skin pretreatment on the volar surface of one forearm. Then, the lidocaine gel soaked in a round sponge was applied on both the pretreated area of the forearm and the unpretreated contrast area of the other forearm. A pain score was obtained from each subject at 30, 45, 60, and 75 min after placement of the Dermal Patch by noting the number of painful pinpricks of five delivered. The mean pain scores on the pretreated side were significantly lower at 30, 45, and 60 min in the stripping study group and at 45 and 60 min in the cleaning study group, compared with the corresponding scores on the unpretreated side (P less than 0.05). Skin pretreatments, such as stripping and cleaning, are useful for shortening the onset of the Dermal Patch anesthesia.

  • Dermal Patch anaesthesia for venous cannulation with 10 lignocaine gel containing glycyrrhetinic acid monohemiphthalate disodium as an absorption promoter
    Anaesthesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Summary The clinical efficacy of transDermal 10% lignocaine gel mixture containing 3% w/w glycyrrhetinic acid monohemiphthalate disodium as an absorption promoter was evaluated at venous cannulation in 17 paediatric (6–11 years) and 17 adult (29–65 years) patients. After about 60 min of occlusive application, the mean (SD) pinprick pain score was 0.7 (0.7) in the paediatric group, compared with 1.4 (1.3) in the adults (p < 0.05). Twenty-five patients (14 children and 11 adults) who had a pinprick score of 0 or 1 underwent venous cannulation without intraDermal local anaesthetic. The mean (SD) pain scores at venepuncture showed no significant differences between children and adults.

  • Skin pretreatments for shortening onset of Dermal Patch anesthesia with 3% GA MHPh 2Na-10% lidocaine gel mixture.
    Anesthesia and analgesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Three types of skin pretreatments (stripping with adhesive tape, scrubbing with Skin-Pure jelly, and cleaning with benzine) were studied in 21 volunteers to determine whether the pretreatments could shorten the application period of transDermal 3% GA MHPh 2Na-10% lidocaine gel. Before application of the gel, each subject received skin pretreatment on the volar surface of one forearm. Then, the lidocaine gel soaked in a round sponge was applied on both the pretreated area of the forearm and the unpretreated contrast area of the other forearm. A pain score was obtained from each subject at 30, 45, 60, and 75 min after placement of the Dermal Patch by noting the number of painful pinpricks of five delivered. The mean pain scores on the pretreated side were significantly lower at 30, 45, and 60 min in the stripping study group and at 45 and 60 min in the cleaning study group, compared with the corresponding scores on the unpretreated side (P less than 0.05). Skin pretreatments, such as stripping and cleaning, are useful for shortening the onset of the Dermal Patch anesthesia.

Kenji Sugibayashi - One of the best experts on this subject based on the ideXlab platform.

  • Preparation and Evaluation of Gene-transfected Cultured Skin as a Novel Drug Delivery System for Severely Burned Skin
    Pharmaceutical Research, 2007
    Co-Authors: Nobuko Hada, Hiroaki Todo, Fusao Komada, Kenji Sugibayashi
    Abstract:

    Purpose The purpose of this study is to prepare and evaluate gene-transfected cultured skin to establish a Dermal Patch consisting of cultured skin as a new and novel delivery system for severely burned skin. Materials and Methods Plasmid DNA encoding the green fluorescent protein (GFP) gene was used as a model gene and transfected to rat and human cultured dermis models (CDMs) using the hemagglutinating virus of Japan envelope vector (HVJ-E) to prepare gene-transfected CDM and evaluate GFP expression in the CDM. Two kinds of transfection methods were evaluated. In pre-transfection, the gene was first transfected into fibroblasts and then CDM was prepared using these gene-transfected cells. In post-transfection, the gene was transfected directly into CDM. Results GFP expression was observed in both the pre- and post-transfected CDMs. The post-transfection method showed higher GFP expression in the CDM than pre-transfection, although no statistically significant difference was observed. The cell viability of these transfected CDMs was also examined with MTT assay. Slight decrease in viability was observed in these transfected CDMs. These methods could be useful in preparing gene-transfected cultured skins with low cell damage. Conclusion Gene transfection to cultured skin may produce several Dermal Patches that release potent endogenous bioactive peptides.

  • Preparation and evaluation of gene-transfected cultured skin as a novel drug delivery system for severely burned skin.
    Pharmaceutical research, 2007
    Co-Authors: Nobuko Hada, Hiroaki Todo, Fusao Komada, Kenji Sugibayashi
    Abstract:

    Purpose The purpose of this study is to prepare and evaluate gene-transfected cultured skin to establish a Dermal Patch consisting of cultured skin as a new and novel delivery system for severely burned skin.

  • Drug Release Test to Assess Quality of Topical Formulations in Japanese Market
    Drug Development and Industrial Pharmacy, 1996
    Co-Authors: Yukiya Yamaguchi, Kenji Sugibayashi, Hiroshi Sato, Yasunori Morimoto
    Abstract:

    AbstractThree release test methods—watch glass method, rotating dialyses cell method, and disk assembly method (DA)—were assessed for use in a quality control procedure for four different semisolid topical formulations containing indomethacin (IDM). Although minor modification in the methods to optimize the release rate was necessary for each formulation, DA proved superior to the other two Methods and thus was used to assess the quality of topical formulations on the Japanese market. First, DA was used for a storage test of hydrophilic ointments and cataplasms; difference in the release profiles of IDM from these formulations showed changes with time, which suggests the usefulness of DA for checking lot-to-lot uniformity. Second, Dermal Patch and tape as transDermal delivery systems containing nitro-glycerin or isosorbide dinitrate were investigated. Since different sizes and shapes of these products are available, various assemblies of DA were required to Pt individual products. Different release patter...

Juwoong Jang - One of the best experts on this subject based on the ideXlab platform.

  • effective healing of chronic rotator cuff injury using recombinant bone morphogenetic protein 2 coated Dermal Patch in vivo
    Journal of Biomedical Materials Research Part B, 2017
    Co-Authors: Youngbock Shim, Juwoong Jang
    Abstract:

    : Biologic augmentation for rotator cuff repair is a challenging treatment in patients with chronic large, massive, and irreparable rotator cuff injuries. Particularly, the use of an extracellular matrix (ECM) Patch such as Dermal tissue offered improved biomechanical properties in previous studies. Cytokines induce cell chemotaxis, proliferation, matrix synthesis, and cell differentiation. Moreover, osteoinductive growth factors such as bone morphogenetic protein-2 (BMP-2) affect the formation of new bone and fibrocartilage in lesions. However, the effects of using a Dermal Patch in combination with BMP-2 have not been evaluated to date, although many researchers have recognized the importance thereof. In this study, rhBMP-2-coated Dermal Patch (1 cm × 2 cm) isolated from human cadaveric donor was inserted in a rabbit model of chronic rotator cuff injury for in vivo evaluation. Bone mineral density and biomechanical strength were tested and histological and histomorphometric analyses were performed. The results showed that insertion of an rhBMP-2-coated acellular Dermal Patch not only significantly ameliorated new bone formation, it also improved biomechanical properties such as ultimate tensile strength. Thus, the use of this combination may improve the chronic rotator cuff injury-healing rate and clinical outcomes after rotator cuff repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1840-1846, 2017.

  • Effective healing of chronic rotator cuff injury using recombinant bone morphogenetic protein‐2 coated Dermal Patch in vivo
    Journal of Biomedical Materials Research Part B, 2016
    Co-Authors: Youngbock Shim, Juwoong Jang
    Abstract:

    Biologic augmentation for rotator cuff repair is a challenging treatment in patients with chronic large, massive, and irreparable rotator cuff injuries. Particularly, the use of an extracellular matrix (ECM) Patch such as Dermal tissue offered improved biomechanical properties in previous studies. Cytokines induce cell chemotaxis, proliferation, matrix synthesis, and cell differentiation. Moreover, osteoinductive growth factors such as bone morphogenetic protein-2 (BMP-2) affect the formation of new bone and fibrocartilage in lesions. However, the effects of using a Dermal Patch in combination with BMP-2 have not been evaluated to date, although many researchers have recognized the importance thereof. In this study, rhBMP-2-coated Dermal Patch (1 cm × 2 cm) isolated from human cadaveric donor was inserted in a rabbit model of chronic rotator cuff injury for in vivo evaluation. Bone mineral density and biomechanical strength were tested and histological and histomorphometric analyses were performed. The results showed that insertion of an rhBMP-2-coated acellular Dermal Patch not only significantly ameliorated new bone formation, it also improved biomechanical properties such as ultimate tensile strength. Thus, the use of this combination may improve the chronic rotator cuff injury-healing rate and clinical outcomes after rotator cuff repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1840-1846, 2017.

Cheryl R Blanchard - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of a cross linked acellular porcine Dermal Patch for rotator cuff repair augmentation in an ovine model
    Journal of Shoulder and Elbow Surgery, 2007
    Co-Authors: Gregory P Nicholson, Gert J Breur, David C Van Sickle, Cheryl R Blanchard
    Abstract:

    In this study we evaluated 2 commercially available rotator cuff repair augmentation Patches in an in vivo sheep model using mechanical testing and histologic techniques. Bilateral infraspinatus tears were created and repaired in 2 groups of 8 adult ewes. Each group (killed at 9 or 24 weeks) included 5 repaired with suture alone, 6 repaired and augmented with a cross-linked acellular porcine Dermal (PD) Patch (Zimmer Collagen Repair Patch), and 5 repaired and augmented with a porcine small intestine submucosa (SIS) Patch (Restore Orthobiologic Soft Tissue Implant; DePuy Orthopaedics). At 3 weeks, sheep with suture repair and an SIS Patch had significant elevation of plasma fibrinogen levels (P

  • Evaluation of a cross-linked acellular porcine Dermal Patch for rotator cuff repair augmentation in an ovine model.
    Journal of shoulder and elbow surgery, 2007
    Co-Authors: Gregory P Nicholson, Gert J Breur, David Van Sickle, Jian Q Yao, J Kim, Cheryl R Blanchard
    Abstract:

    In this study we evaluated 2 commercially available rotator cuff repair augmentation Patches in an in vivo sheep model using mechanical testing and histologic techniques. Bilateral infraspinatus tears were created and repaired in 2 groups of 8 adult ewes. Each group (killed at 9 or 24 weeks) included 5 repaired with suture alone, 6 repaired and augmented with a cross-linked acellular porcine Dermal (PD) Patch (Zimmer Collagen Repair Patch), and 5 repaired and augmented with a porcine small intestine submucosa (SIS) Patch (Restore Orthobiologic Soft Tissue Implant; DePuy Orthopaedics). At 3 weeks, sheep with suture repair and an SIS Patch had significant elevation of plasma fibrinogen levels (P < .05) whereas sheep with suture repair and a PD Patch elicited no elevation in plasma fibrinogen levels. At 9 weeks, the mean failure load was 201 +/- 60 lb for suture repairs, 182 +/- 63 lb for PD repairs, and 137 +/- 16 lb for SIS repairs. Within any individual sheep, the shoulder undergoing PD repair always had a higher failure load than the contralateral suture or shoulder undergoing SIS repair. At 9 weeks, macrophages were seen on all PD surfaces whereas most of the SIS materials were resorbed. At 24 weeks, failure loads were identical between groups. Macrophages had disappeared from the PD groups, and integration of the PD Patch into the surrounding tissue with vascular and fibroblastic invasion was seen. For the SIS group, diverse tissue types (including ectopic bone) were seen.

Tatsuhiko Kano - One of the best experts on this subject based on the ideXlab platform.

  • Dermal Patch anaesthesia comparison of 10 lignocaine gel with absorption promoter and emla cream
    Anaesthesia, 1993
    Co-Authors: Masakatsu Sakamoto, Tatsuhiko Kano, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Osamu Shimoda, Masahiro Nakano
    Abstract:

    The analgesic effects of transDermally applied 10% lignocaine aquagel containing 3% glycyrrhetinic acid monohemiphthalate disodium (as an absorption enhancer) and EMLA cream were compared on the forearms of 34 adult volunteers in a double-blind fashion. The mean pinprick pain scores (graded by noting the number of painful pinpricks out of five) at 30, 60 and 90 min after application and 30 min after removal of the anaesthetics were 3.3 (0.3) (mean SE), 1.2 (0.3), 0.3 (0.1) and 0.3 (0.1) respectively, in the lignocaine gel group. Corresponding scores were 3.5 (0.3), 1.5 (0.3), 0.7 (0.2) and 0.1 (0.1) respectively, in the EMLA group. Insertion of a 26-gauge needle into the treated skin to a depth of 1 mm at 90 min after application was not painful in 91% of the volunteers in the lignocaine gel group and 88% of those in the EMLA group. There was no significant difference in any of the corresponding pain scores between the two groups.

  • Dermal Patch anaesthesia: comparison of 10% lignocaine gel with absorption promoter and EMLA cream
    Anaesthesia, 1993
    Co-Authors: Masakatsu Sakamoto, Tatsuhiko Kano, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Osamu Shimoda, Masahiro Nakano
    Abstract:

    The analgesic effects of transDermally applied 10% lignocaine aquagel containing 3% glycyrrhetinic acid monohemiphthalate disodium (as an absorption enhancer) and EMLA cream were compared on the forearms of 34 adult volunteers in a double-blind fashion. The mean pinprick pain scores (graded by noting the number of painful pinpricks out of five) at 30, 60 and 90 min after application and 30 min after removal of the anaesthetics were 3.3 (0.3) (mean SE), 1.2 (0.3), 0.3 (0.1) and 0.3 (0.1) respectively, in the lignocaine gel group. Corresponding scores were 3.5 (0.3), 1.5 (0.3), 0.7 (0.2) and 0.1 (0.1) respectively, in the EMLA group. Insertion of a 26-gauge needle into the treated skin to a depth of 1 mm at 90 min after application was not painful in 91% of the volunteers in the lignocaine gel group and 88% of those in the EMLA group. There was no significant difference in any of the corresponding pain scores between the two groups.

  • skin pretreatments for shortening onset of Dermal Patch anesthesia with 3 ga mhph 2na 10 lidocaine gel mixture
    Anesthesia & Analgesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Three types of skin pretreatments (stripping with adhesive tape, scrubbing with Skin-Pure jelly, and cleaning with benzine) were studied in 21 volunteers to determine whether the pretreatments could shorten the application period of transDermal 3% GA MHPh 2Na-10% lidocaine gel. Before application of the gel, each subject received skin pretreatment on the volar surface of one forearm. Then, the lidocaine gel soaked in a round sponge was applied on both the pretreated area of the forearm and the unpretreated contrast area of the other forearm. A pain score was obtained from each subject at 30, 45, 60, and 75 min after placement of the Dermal Patch by noting the number of painful pinpricks of five delivered. The mean pain scores on the pretreated side were significantly lower at 30, 45, and 60 min in the stripping study group and at 45 and 60 min in the cleaning study group, compared with the corresponding scores on the unpretreated side (P less than 0.05). Skin pretreatments, such as stripping and cleaning, are useful for shortening the onset of the Dermal Patch anesthesia.

  • Dermal Patch anaesthesia for venous cannulation with 10 lignocaine gel containing glycyrrhetinic acid monohemiphthalate disodium as an absorption promoter
    Anaesthesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Summary The clinical efficacy of transDermal 10% lignocaine gel mixture containing 3% w/w glycyrrhetinic acid monohemiphthalate disodium as an absorption promoter was evaluated at venous cannulation in 17 paediatric (6–11 years) and 17 adult (29–65 years) patients. After about 60 min of occlusive application, the mean (SD) pinprick pain score was 0.7 (0.7) in the paediatric group, compared with 1.4 (1.3) in the adults (p < 0.05). Twenty-five patients (14 children and 11 adults) who had a pinprick score of 0 or 1 underwent venous cannulation without intraDermal local anaesthetic. The mean (SD) pain scores at venepuncture showed no significant differences between children and adults.

  • Skin pretreatments for shortening onset of Dermal Patch anesthesia with 3% GA MHPh 2Na-10% lidocaine gel mixture.
    Anesthesia and analgesia, 1992
    Co-Authors: Tatsuhiko Kano, Mari Nakamura, Akira Hashiguchi, Michiaki Sadanaga, Tohru Morioka, Motohiro Mishima, Masahiro Nakano
    Abstract:

    Three types of skin pretreatments (stripping with adhesive tape, scrubbing with Skin-Pure jelly, and cleaning with benzine) were studied in 21 volunteers to determine whether the pretreatments could shorten the application period of transDermal 3% GA MHPh 2Na-10% lidocaine gel. Before application of the gel, each subject received skin pretreatment on the volar surface of one forearm. Then, the lidocaine gel soaked in a round sponge was applied on both the pretreated area of the forearm and the unpretreated contrast area of the other forearm. A pain score was obtained from each subject at 30, 45, 60, and 75 min after placement of the Dermal Patch by noting the number of painful pinpricks of five delivered. The mean pain scores on the pretreated side were significantly lower at 30, 45, and 60 min in the stripping study group and at 45 and 60 min in the cleaning study group, compared with the corresponding scores on the unpretreated side (P less than 0.05). Skin pretreatments, such as stripping and cleaning, are useful for shortening the onset of the Dermal Patch anesthesia.