The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform

Eric C. Vonderheid - One of the best experts on this subject based on the ideXlab platform.

  • Research Article Expression of Thymidine Phosphorylase in Lymph Nodes Involved with
    2013
    Co-Authors: Mycosis Fungoides, Xingcao Nie, Rekha Bhat, Essel Dulaimi Al-saleem, Eric C. Vonderheid, Sézary Syndrome, Steve J. Hou
    Abstract:

    which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Thymidine phosphorylase may be overexpressed in both neoplastic cells and tumor stromal cells in a variety of malignancies. Our study explores thymidine phosphorylase expression in lymph nodes (LNs) from patients with mycosis fungoides (MF) or Sézary syndrome (SS). In MF/SS, the LNs may have a pathologic diagnosis of either Dermatopathic Lymphadenopathy (LN-DL) or involvement by MF/SS (LN-MF). We performed immunohistochemical staining on MF/SS lymph nodes using antibodies to thymidine phosphorylase, CD68, CD21, CD3, and CD4. In both LN-DL and benign nodes, thymidine phosphorylase staining was noted only in macrophages, dendritic cells, and endothelial cells. In LN-MF, thymidine phosphorylase expression was also noted in subsets of intermediate to large neoplastic T cells. Concurrent CD68, CD21, CD3, and CD4 staining supported the above observations. Similar results were noted in the skin and in LN-MF with large cell transformation. Other T-cell lymphomas were also examined (total 7 cases); only enteropathy-type T-cell lymphoma (1 case) showed TP positivity in neoplastic T lymphocytes. We demonstrated that thymidine phosphorylase staining is present in neoplastic T cells in mycosis fungoides. The exact mechanism needs further investigation. 1

  • Expression of Thymidine Phosphorylase in Lymph Nodes Involved with Mycosis Fungoides and Sézary Syndrome
    Hindawi Limited, 2011
    Co-Authors: Xingcao Nie, Rekha Bhat, Essel Dulaimi Al-saleem, Eric C. Vonderheid, Steve J. Hou
    Abstract:

    Thymidine phosphorylase may be overexpressed in both neoplastic cells and tumor stromal cells in a variety of malignancies. Our study explores thymidine phosphorylase expression in lymph nodes (LNs) from patients with mycosis fungoides (MF) or Sézary syndrome (SS). In MF/SS, the LNs may have a pathologic diagnosis of either Dermatopathic Lymphadenopathy (LN-DL) or involvement by MF/SS (LN-MF). We performed immunohistochemical staining on MF/SS lymph nodes using antibodies to thymidine phosphorylase, CD68, CD21, CD3, and CD4. In both LN-DL and benign nodes, thymidine phosphorylase staining was noted only in macrophages, dendritic cells, and endothelial cells. In LN-MF, thymidine phosphorylase expression was also noted in subsets of intermediate to large neoplastic T cells. Concurrent CD68, CD21, CD3, and CD4 staining supported the above observations. Similar results were noted in the skin and in LN-MF with large cell transformation. Other T-cell lymphomas were also examined (total 7 cases); only enteropathy-type T-cell lymphoma (1 case) showed TP positivity in neoplastic T lymphocytes. We demonstrated that thymidine phosphorylase staining is present in neoplastic T cells in mycosis fungoides. The exact mechanism needs further investigation

  • fine needle aspiration biopsy in the evaluation of Lymphadenopathy associated with cutaneous t cell lymphoma mycosis fungoides sezary syndrome
    American Journal of Clinical Pathology, 2000
    Co-Authors: Lorenzo M Galindo, Fernando U Garcia, Cheryl A Hanau, Stuart R Lessin, Nirag Jhala, Robert D Bigler, Eric C. Vonderheid
    Abstract:

    We studied the role of fine-needle aspiration (FNA) in the evaluation of Lymphadenopathy associated with cutaneous T-cell lymphoma (CTCL) in 11 patients with Lymphadenopathy and compared findings with corresponding histologic material. Molecular genetic analysis for T-cell clonality by polymerase chain reaction (PCR) was performed on all aspirates. Immunophenotyping was successful in 4 of 7 cases in which flow cytometry was attempted from the aspirated material. Cytologic evaluation of FNA samples correlated strongly with histologic rating of involvement based on numbers of atypical cerebriform lymphocytes in the nodal specimen. Of 7 nodal specimens with scattered or small groups of atypical cells in the background of Dermatopathic Lymphadenopathy (LN1-2), the cytologic diagnosis was interpreted as reactive in all instances. Of 4 specimens with highly suspect (LN3) or definite histologic involvement (LN4), the cytologic diagnosis was likewise suspect or malignant. The correlation between molecular genetic studies on FNA samples and studies on tissue was not significant; in 2 cases, a T-cell clone was detected in the nodal tissue sample but not in the FNA sample, suggesting undersampling. A T-cell clone was detected by PCR in 5 of 7 nodal specimens judged reactive by FNA biopsy or histologic assessment. FNA for cytologic and molecular genetic analysis is a useful method to evaluate Lymphadenopathy associated with CTCL and may obviate the need for surgical biopsy.

Haruki Wakasa - One of the best experts on this subject based on the ideXlab platform.

  • temporal differences of onset between primary skin lesions and regional lymph node lesions for tularemia in japan a clinicopathologic and immunohistochemical study of 19 skin cases and 54 lymph node cases
    Virchows Archiv, 2012
    Co-Authors: Shigeyuki Asano, Kikuo Mori, Kazuki Yamazaki, Tetsutaro Sata, Takayuki Kanno, Yuko Sato, Masaru Kojima, Hiromi Fujita, Yasushi Akaike, Haruki Wakasa
    Abstract:

    For tularemia, a zoonosis caused by the gram-negative coccobacillus Francisella tularensis, research of the relation between skin lesions and lymph node lesions has not been reported in the literature. This report describes skin lesions and lymph node lesions and their mutual relation over time for tularemia in Japan. Around the second day after infection (DAI), a subcutaneous abscess was observed (abscess form). Hand and finger skin ulcers formed during the second to the fourth week. Subcutaneous and dermal granulomas were observed with adjacent monocytoid B lymphocytes (MBLs) (abscess–granulomatous form). From the sixth week, large granulomas with central homogeneous lesions emerged diffusely (granulomatous form). On 2–14 DAI, F. tularensis antigen in skin lesions was detected in abscesses. During 7–12 DAI, abscesses with adjacent MBLs appeared without epithelioid granuloma (abscess form) in regional lymph nodes. During the second to fifth week, granulomas appeared with necrosis (abscess–granulomatous form). After the sixth week, large granulomas with a central homogeneous lesion (granulomatous form) appeared. F. tularensis antigen in lymph node lesions was observed in the abscess on 7–92 DAI. Apparently, F. tularensis penetrates the finger skin immediately after contact with infected hares. Subsequently, the primary lesion gradually transfers from skin to regional lymph nodes. The regional lymph node lesions induced by skin lesion are designated as Dermatopathic Lymphadenopathy. This study revealed temporal differences of onset among the skin and lymph node lesions.

Shigeyuki Asano - One of the best experts on this subject based on the ideXlab platform.

  • temporal differences of onset between primary skin lesions and regional lymph node lesions for tularemia in japan a clinicopathologic and immunohistochemical study of 19 skin cases and 54 lymph node cases
    Virchows Archiv, 2012
    Co-Authors: Shigeyuki Asano, Kikuo Mori, Kazuki Yamazaki, Tetsutaro Sata, Takayuki Kanno, Yuko Sato, Masaru Kojima, Hiromi Fujita, Yasushi Akaike, Haruki Wakasa
    Abstract:

    For tularemia, a zoonosis caused by the gram-negative coccobacillus Francisella tularensis, research of the relation between skin lesions and lymph node lesions has not been reported in the literature. This report describes skin lesions and lymph node lesions and their mutual relation over time for tularemia in Japan. Around the second day after infection (DAI), a subcutaneous abscess was observed (abscess form). Hand and finger skin ulcers formed during the second to the fourth week. Subcutaneous and dermal granulomas were observed with adjacent monocytoid B lymphocytes (MBLs) (abscess–granulomatous form). From the sixth week, large granulomas with central homogeneous lesions emerged diffusely (granulomatous form). On 2–14 DAI, F. tularensis antigen in skin lesions was detected in abscesses. During 7–12 DAI, abscesses with adjacent MBLs appeared without epithelioid granuloma (abscess form) in regional lymph nodes. During the second to fifth week, granulomas appeared with necrosis (abscess–granulomatous form). After the sixth week, large granulomas with a central homogeneous lesion (granulomatous form) appeared. F. tularensis antigen in lymph node lesions was observed in the abscess on 7–92 DAI. Apparently, F. tularensis penetrates the finger skin immediately after contact with infected hares. Subsequently, the primary lesion gradually transfers from skin to regional lymph nodes. The regional lymph node lesions induced by skin lesion are designated as Dermatopathic Lymphadenopathy. This study revealed temporal differences of onset among the skin and lymph node lesions.

Lorenzo M Galindo - One of the best experts on this subject based on the ideXlab platform.

  • fine needle aspiration biopsy in the evaluation of Lymphadenopathy associated with cutaneous t cell lymphoma mycosis fungoides sezary syndrome
    American Journal of Clinical Pathology, 2000
    Co-Authors: Lorenzo M Galindo, Fernando U Garcia, Cheryl A Hanau, Stuart R Lessin, Nirag Jhala, Robert D Bigler, Eric C. Vonderheid
    Abstract:

    We studied the role of fine-needle aspiration (FNA) in the evaluation of Lymphadenopathy associated with cutaneous T-cell lymphoma (CTCL) in 11 patients with Lymphadenopathy and compared findings with corresponding histologic material. Molecular genetic analysis for T-cell clonality by polymerase chain reaction (PCR) was performed on all aspirates. Immunophenotyping was successful in 4 of 7 cases in which flow cytometry was attempted from the aspirated material. Cytologic evaluation of FNA samples correlated strongly with histologic rating of involvement based on numbers of atypical cerebriform lymphocytes in the nodal specimen. Of 7 nodal specimens with scattered or small groups of atypical cells in the background of Dermatopathic Lymphadenopathy (LN1-2), the cytologic diagnosis was interpreted as reactive in all instances. Of 4 specimens with highly suspect (LN3) or definite histologic involvement (LN4), the cytologic diagnosis was likewise suspect or malignant. The correlation between molecular genetic studies on FNA samples and studies on tissue was not significant; in 2 cases, a T-cell clone was detected in the nodal tissue sample but not in the FNA sample, suggesting undersampling. A T-cell clone was detected by PCR in 5 of 7 nodal specimens judged reactive by FNA biopsy or histologic assessment. FNA for cytologic and molecular genetic analysis is a useful method to evaluate Lymphadenopathy associated with CTCL and may obviate the need for surgical biopsy.

Steve J. Hou - One of the best experts on this subject based on the ideXlab platform.

  • Research Article Expression of Thymidine Phosphorylase in Lymph Nodes Involved with
    2013
    Co-Authors: Mycosis Fungoides, Xingcao Nie, Rekha Bhat, Essel Dulaimi Al-saleem, Eric C. Vonderheid, Sézary Syndrome, Steve J. Hou
    Abstract:

    which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Thymidine phosphorylase may be overexpressed in both neoplastic cells and tumor stromal cells in a variety of malignancies. Our study explores thymidine phosphorylase expression in lymph nodes (LNs) from patients with mycosis fungoides (MF) or Sézary syndrome (SS). In MF/SS, the LNs may have a pathologic diagnosis of either Dermatopathic Lymphadenopathy (LN-DL) or involvement by MF/SS (LN-MF). We performed immunohistochemical staining on MF/SS lymph nodes using antibodies to thymidine phosphorylase, CD68, CD21, CD3, and CD4. In both LN-DL and benign nodes, thymidine phosphorylase staining was noted only in macrophages, dendritic cells, and endothelial cells. In LN-MF, thymidine phosphorylase expression was also noted in subsets of intermediate to large neoplastic T cells. Concurrent CD68, CD21, CD3, and CD4 staining supported the above observations. Similar results were noted in the skin and in LN-MF with large cell transformation. Other T-cell lymphomas were also examined (total 7 cases); only enteropathy-type T-cell lymphoma (1 case) showed TP positivity in neoplastic T lymphocytes. We demonstrated that thymidine phosphorylase staining is present in neoplastic T cells in mycosis fungoides. The exact mechanism needs further investigation. 1

  • Expression of Thymidine Phosphorylase in Lymph Nodes Involved with Mycosis Fungoides and Sézary Syndrome
    Hindawi Limited, 2011
    Co-Authors: Xingcao Nie, Rekha Bhat, Essel Dulaimi Al-saleem, Eric C. Vonderheid, Steve J. Hou
    Abstract:

    Thymidine phosphorylase may be overexpressed in both neoplastic cells and tumor stromal cells in a variety of malignancies. Our study explores thymidine phosphorylase expression in lymph nodes (LNs) from patients with mycosis fungoides (MF) or Sézary syndrome (SS). In MF/SS, the LNs may have a pathologic diagnosis of either Dermatopathic Lymphadenopathy (LN-DL) or involvement by MF/SS (LN-MF). We performed immunohistochemical staining on MF/SS lymph nodes using antibodies to thymidine phosphorylase, CD68, CD21, CD3, and CD4. In both LN-DL and benign nodes, thymidine phosphorylase staining was noted only in macrophages, dendritic cells, and endothelial cells. In LN-MF, thymidine phosphorylase expression was also noted in subsets of intermediate to large neoplastic T cells. Concurrent CD68, CD21, CD3, and CD4 staining supported the above observations. Similar results were noted in the skin and in LN-MF with large cell transformation. Other T-cell lymphomas were also examined (total 7 cases); only enteropathy-type T-cell lymphoma (1 case) showed TP positivity in neoplastic T lymphocytes. We demonstrated that thymidine phosphorylase staining is present in neoplastic T cells in mycosis fungoides. The exact mechanism needs further investigation