The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform
Kazutaka Soejima - One of the best experts on this subject based on the ideXlab platform.
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one step grafting procedure using artificial Dermis and split thickness skin in burn patients
European Journal of Plastic Surgery, 2013Co-Authors: Kazutaka Soejima, Takashi Yamaki, Taro Kono, Tsutomu Kashimura, Katsumi Shimoda, Hiroyuki Sakurai, Hiroaki NakazawaAbstract:The study aimed to achieve a one-step grafting procedure using artificial Dermis and split-thickness skin. We performed simultaneous grafting of artificial Dermis and skin in two severely burned patients. Artificial Dermis was treated with fresh autogenous platelet-derived wound-healing factors (PDWHF), cryopreserved allogeneic cultured endothelial cells, and fibroblasts. Dermal microvascular endothelial cells and fibroblasts were obtained from a single human donor’s skin. The cultured cells were cryopreserved until use in grafting. The PDWHF was prepared from autogenous blood from each patient prior to the surgery. In two patients, the artificial Dermis treated with this method was grafted to a full-thickness burn wound. Immediately after artificial Dermis grafting, meshed split-thickness skin was grafted. In each case, the skin graft took well, and the skin texture was acceptable. Histological examination revealed that bovine collagen tissue remained in the Dermis after surgery, indicating the success of the simultaneous grafting of the artificial Dermis and the skin. The present study indicates that one-step grafting of artificial Dermis and split-skin is possible when the artificial Dermis is treated with PDWHF and cultured endothelial cells and fibroblasts.
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novel application method of artificial Dermis one step grafting procedure of artificial Dermis and skin rat experimental study
Burns, 2006Co-Authors: Kazutaka Soejima, Keijiro Hori, Hiroyuki Sakurai, Motohiro Nozaki, Xin Chen, Masaki TakeuchiAbstract:Abstract Background Currently, to treat skin defects with artificial Dermis (AD), two surgical procedures where the artificial Dermis grafting and another secondary skin grafting are required. The purpose of this study was to achieve simultaneous grafting of the artificial Dermis and the split-skin. To enhance the wound angiogenesis, cultured endothelial cells, fibroblasts and PDWHF (platelet derived wound healing factor) were employed. Methods The experiment consists of following two parts: (1) Investigation to obtain faster angiogenesis into the bilayer artificial Dermis: full-thickness wounds created on the back of the rats were treated with the artificial Dermis (TeruDermis ® , with silicone sheet, TERUMO Co., Japan). Prior to the artificial Dermis grafting, following four groups were established; control group (AD alone, n = 6), PDWHF group (AD treated with PDWHF, n = 6), cultured cells group (AD treated with cultured endothelial cells and fibroblasts, n = 6), combination group (AD treated with PDWHF and cultured cells, n = 6). (2) Trial of one-stage grafting of the AD and the skin: simultaneous grafting of the artificial Dermis and skin was performed using the same rat model. Before making skin defects, split thickness skin were harvested. Then the skin grafting was carried out immediately after the AD grafting. To allow grafting of the skin onto the artificial Dermis, the AD without silicone sheet (TeruDermis ® without silicone sheet, TERUMO Co., Japan) were used. Two groups, control group (AD alone, n = 3) and treatment group (AD with PDWF and cultures, n = 3) were established. Results (1) When the artificial Dermis were treated with PDWHF, cultured endothelial cells and fibroblasts, vascular invasion into the artificial Dermis was observed 5 days after the surgery. (2) In the treatment group, the skin grafted immediately after the artificial Dermis grafting was completely taken. Conclusions The present study revealed that treatment with PDWHF, combined with cultured endothelial cells and fibroblasts, accelerated wound angiogenesis. By this method, one-step grafting procedure of the artificial Dermis and the skin is possible.
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treatment of giant pigmented nevus using artificial Dermis and a secondary skin graft from the scalp
Annals of Plastic Surgery, 1997Co-Authors: Kazutaka Soejima, Kenji Sasaki, Motohiro Nozaki, Masaki Takeuchi, Naoki NegishiAbstract:: From January 1994 to October 1995, 5 patients with congenital giant pigmented nevi were treated using artificial Dermis with a secondary skin graft from the scalp. The nevus was excised in full thickness and the open wound was grafted with artificial Dermis. About 3 weeks later, thin split-thickness skin grafting on the newly synthesized, Dermis-like tissue was required. We chose the scalp as the donor of the secondary skin graft. Epithelialization of each donor site was completed within 1 week. In addition, the donor site was not complicated with alopecia or hypertrophic scars. Morbidity at the donor site was minimized and favorable tissue quality of the grafted skin was obtained. The clinical results revealed that the scalp was a favorable donor of a secondary skin graft for the treatment of giant pigmented nevus with artificial Dermis.
Masaki Takeuchi - One of the best experts on this subject based on the ideXlab platform.
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novel application method of artificial Dermis one step grafting procedure of artificial Dermis and skin rat experimental study
Burns, 2006Co-Authors: Kazutaka Soejima, Keijiro Hori, Hiroyuki Sakurai, Motohiro Nozaki, Xin Chen, Masaki TakeuchiAbstract:Abstract Background Currently, to treat skin defects with artificial Dermis (AD), two surgical procedures where the artificial Dermis grafting and another secondary skin grafting are required. The purpose of this study was to achieve simultaneous grafting of the artificial Dermis and the split-skin. To enhance the wound angiogenesis, cultured endothelial cells, fibroblasts and PDWHF (platelet derived wound healing factor) were employed. Methods The experiment consists of following two parts: (1) Investigation to obtain faster angiogenesis into the bilayer artificial Dermis: full-thickness wounds created on the back of the rats were treated with the artificial Dermis (TeruDermis ® , with silicone sheet, TERUMO Co., Japan). Prior to the artificial Dermis grafting, following four groups were established; control group (AD alone, n = 6), PDWHF group (AD treated with PDWHF, n = 6), cultured cells group (AD treated with cultured endothelial cells and fibroblasts, n = 6), combination group (AD treated with PDWHF and cultured cells, n = 6). (2) Trial of one-stage grafting of the AD and the skin: simultaneous grafting of the artificial Dermis and skin was performed using the same rat model. Before making skin defects, split thickness skin were harvested. Then the skin grafting was carried out immediately after the AD grafting. To allow grafting of the skin onto the artificial Dermis, the AD without silicone sheet (TeruDermis ® without silicone sheet, TERUMO Co., Japan) were used. Two groups, control group (AD alone, n = 3) and treatment group (AD with PDWF and cultures, n = 3) were established. Results (1) When the artificial Dermis were treated with PDWHF, cultured endothelial cells and fibroblasts, vascular invasion into the artificial Dermis was observed 5 days after the surgery. (2) In the treatment group, the skin grafted immediately after the artificial Dermis grafting was completely taken. Conclusions The present study revealed that treatment with PDWHF, combined with cultured endothelial cells and fibroblasts, accelerated wound angiogenesis. By this method, one-step grafting procedure of the artificial Dermis and the skin is possible.
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treatment of giant pigmented nevus using artificial Dermis and a secondary skin graft from the scalp
Annals of Plastic Surgery, 1997Co-Authors: Kazutaka Soejima, Kenji Sasaki, Motohiro Nozaki, Masaki Takeuchi, Naoki NegishiAbstract:: From January 1994 to October 1995, 5 patients with congenital giant pigmented nevi were treated using artificial Dermis with a secondary skin graft from the scalp. The nevus was excised in full thickness and the open wound was grafted with artificial Dermis. About 3 weeks later, thin split-thickness skin grafting on the newly synthesized, Dermis-like tissue was required. We chose the scalp as the donor of the secondary skin graft. Epithelialization of each donor site was completed within 1 week. In addition, the donor site was not complicated with alopecia or hypertrophic scars. Morbidity at the donor site was minimized and favorable tissue quality of the grafted skin was obtained. The clinical results revealed that the scalp was a favorable donor of a secondary skin graft for the treatment of giant pigmented nevus with artificial Dermis.
M. Horsmanheimo - One of the best experts on this subject based on the ideXlab platform.
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quantitative analysis of tryptase and chymase containing mast cells in atopic dermatitis and nummular eczema
British Journal of Dermatology, 1997Co-Authors: Anitta Jarvikallio, M. -l. Aalto, Anita Naukkarinen, Ilkka T Harvima, M. HorsmanheimoAbstract:Summary The distribution of mast cells (MCs) containing tryptase (T) and chymase (C) was studied in the non-lesional and lesional skin of 26 patients with atopic dertnatitis (AD) and 23 patients with non-atopic nummular eczema (NE). and in the skin of eight healthy controls. T and C activities were demonstrated enzymehistochemically using 2-Gly-Pro-Arg-MNA and Suc-Val-Pro-Phe-MNA as substrates, respectively. The T- and C-containing MCs were counted separately in the epiDermis, in contact with the basement membrane. In the papillary Dermis and in different dermal levels (0·2 mm each). Also, the C protein was determined immunohistochemically. T-positive MCs were similarly distributed in non-lesional and lesional skin of both AD and NE. The MC number was relatively high in the upper Dermis (papillary Dermis and levels I and I!) of non-lesional and lesional skin of AD. In the upper Dermis of non-lesional AD and NE skin and in normal skin, about 50% of T-positive MCs displayed C activity, whereas the percentage in lesional AD and NE skin was only about 30%. hi this respect, the non-lesional and lesional samples differed significantly froLu each other in both dennatoses (in AD p = 0%005, in NEP = 0·002. Students' t-test). In all samples the MC number decreased in the deeper dermal levels, although numerous T-containing MCs were still counted in the deeper Dermis (dermal levels IV-VII) of lesional AD and NE skiti. differing significantly from the MC number in normal skin (In ADp = 0·005. in NE p=0·041). In the deeper Dermis. the percentage of MCs containing active C was about 70% in non-lesional and lesional AD and NE. and about 90% in normal healthy skin. However, in the upper Dermis of non-lesional and lesional skin of both AD and NE. about H()% of all MCs contained the C protein, which differed significantly from the value of 100% in normal skin (p<0·5). In conclusion, the increased number of T-positive MCs in the upper Dermis of non-lesional and lesional AD contributes to promoting inflammation. C apparently loses its activity in the upper Dermis of lesional AD and especially in NE. Thus. Ihe enzyme partially lacks its capability to suppress inflammation, such as degradation of neuropeptides and proteins. The dysregulation of these proteinases exists already in non-lesional skin of AD and NE.
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Quantitative enzyme-histochemical analysis of tryptase- and chymase-containing mast cells in psoriatic skin
Archives of Dermatological Research, 1990Co-Authors: I. T. Harvima, A. Naukkarinen, R. J. Harvima, M. -l. Aalto, H. NeittaanmÄki, M. HorsmanheimoAbstract:Tryptase-containing mast cells have recently been found to be increased in the upper Dermis of psoriatic lesions. In the present study, the distribution of chymaseand tryptase-containing mast cells was morphometrically analysed at different dermal levels of lesional and non-lesional psoriatic skin (12 patients) as well as normal human skin. Mast cell tryptase was identified enzyme-histochemically, using Z-Gly-Pro-Arg-MNA as the substrate. For demonstrating mast cell chymase, a simple and specific enzyme-histochemical staining method was developed, using Suc-Val-Pro-Phe-MNA as the substrate. All mast cells positive for chymase were also positive for tryptase and Giemsa stain. Although the number of tryptase-positive mast cells was slightly increased throughout the Dermis of lesional psoriatic skin, this increase was most pronounced in the upper Dermis immediately beneath, and in close contact with, the epiDermis. In contrast, the number of chymase-positive mast cells was clearly decreased in the upper Dermis of psoriatic lesions, but not in the deeper Dermis, as compared with non-lesional psoriatic skin. In addition, all chymase-positive mast cells observed in the upper Dermis were very weakly stained when compared with those in the deeper Dermis. No differences were found between non-lesional psoriatic skin and normal skin in which the number of mast cells containing chymase was 72–73% of the number containing tryptase. The present results suggest that T mast cells particularly, containing tryptase but no chymase, proliferate in psoriatic lesions, and that the increase in tryptase activity and the decrease in chymase activitiy in the upper Dermis may lead to an imbalance in the biochemical regulatory systems.
Hiroyuki Sakurai - One of the best experts on this subject based on the ideXlab platform.
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one step grafting procedure using artificial Dermis and split thickness skin in burn patients
European Journal of Plastic Surgery, 2013Co-Authors: Kazutaka Soejima, Takashi Yamaki, Taro Kono, Tsutomu Kashimura, Katsumi Shimoda, Hiroyuki Sakurai, Hiroaki NakazawaAbstract:The study aimed to achieve a one-step grafting procedure using artificial Dermis and split-thickness skin. We performed simultaneous grafting of artificial Dermis and skin in two severely burned patients. Artificial Dermis was treated with fresh autogenous platelet-derived wound-healing factors (PDWHF), cryopreserved allogeneic cultured endothelial cells, and fibroblasts. Dermal microvascular endothelial cells and fibroblasts were obtained from a single human donor’s skin. The cultured cells were cryopreserved until use in grafting. The PDWHF was prepared from autogenous blood from each patient prior to the surgery. In two patients, the artificial Dermis treated with this method was grafted to a full-thickness burn wound. Immediately after artificial Dermis grafting, meshed split-thickness skin was grafted. In each case, the skin graft took well, and the skin texture was acceptable. Histological examination revealed that bovine collagen tissue remained in the Dermis after surgery, indicating the success of the simultaneous grafting of the artificial Dermis and the skin. The present study indicates that one-step grafting of artificial Dermis and split-skin is possible when the artificial Dermis is treated with PDWHF and cultured endothelial cells and fibroblasts.
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novel application method of artificial Dermis one step grafting procedure of artificial Dermis and skin rat experimental study
Burns, 2006Co-Authors: Kazutaka Soejima, Keijiro Hori, Hiroyuki Sakurai, Motohiro Nozaki, Xin Chen, Masaki TakeuchiAbstract:Abstract Background Currently, to treat skin defects with artificial Dermis (AD), two surgical procedures where the artificial Dermis grafting and another secondary skin grafting are required. The purpose of this study was to achieve simultaneous grafting of the artificial Dermis and the split-skin. To enhance the wound angiogenesis, cultured endothelial cells, fibroblasts and PDWHF (platelet derived wound healing factor) were employed. Methods The experiment consists of following two parts: (1) Investigation to obtain faster angiogenesis into the bilayer artificial Dermis: full-thickness wounds created on the back of the rats were treated with the artificial Dermis (TeruDermis ® , with silicone sheet, TERUMO Co., Japan). Prior to the artificial Dermis grafting, following four groups were established; control group (AD alone, n = 6), PDWHF group (AD treated with PDWHF, n = 6), cultured cells group (AD treated with cultured endothelial cells and fibroblasts, n = 6), combination group (AD treated with PDWHF and cultured cells, n = 6). (2) Trial of one-stage grafting of the AD and the skin: simultaneous grafting of the artificial Dermis and skin was performed using the same rat model. Before making skin defects, split thickness skin were harvested. Then the skin grafting was carried out immediately after the AD grafting. To allow grafting of the skin onto the artificial Dermis, the AD without silicone sheet (TeruDermis ® without silicone sheet, TERUMO Co., Japan) were used. Two groups, control group (AD alone, n = 3) and treatment group (AD with PDWF and cultures, n = 3) were established. Results (1) When the artificial Dermis were treated with PDWHF, cultured endothelial cells and fibroblasts, vascular invasion into the artificial Dermis was observed 5 days after the surgery. (2) In the treatment group, the skin grafted immediately after the artificial Dermis grafting was completely taken. Conclusions The present study revealed that treatment with PDWHF, combined with cultured endothelial cells and fibroblasts, accelerated wound angiogenesis. By this method, one-step grafting procedure of the artificial Dermis and the skin is possible.
Lester Packer - One of the best experts on this subject based on the ideXlab platform.
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enzymic and non enzymic antioxidants in epiDermis and Dermis of human skin
Journal of Investigative Dermatology, 1994Co-Authors: Yasuko Shindo, William L. Epstein, Eric Witt, Lester PackerAbstract:We measured enzymic and non-enzymic antioxidants in human epiDermis and Dermis from six healthy volunteers undergoing surgical procedures. EpiDermis was separated from Dermis by currettage and antioxidants were measured by high-performance liquid chromatography (HPLC) or standard spectrophotometric methods. The concentration of every antioxidant (referenced to skin wet weight) was higher in the epiDermis than in the Dermis. Among the enzymic antioxidants, the activities of superoxide dismutase, glutathione peroxidase, and glutathione reductase were higher in the epiDermis compared to the Dermis by 126, 61 and 215%, respectively. Catalase activity in particular was much higher (720%) in the epiDermis. Glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase, which provide reduced nicotinamide adenine dinucleotide phosphate (NADPH), also showed higher activity in the epiDermis than the Dermis by 111% and 313%, respectively. Among the lipophilic antioxidants, the concentration of α-tocopherol was higher in the epiDermis than the Dermis by 90%. The concentration of ubiquinol 10 was especially higher in the epiDermis, by 900%. Among the hydrophilic antioxidants, concentrations of ascorbic acid and uric acid were also higher in the epiDermis than in the Dermis by 425 and 488%, respectively. Reduced glutathione and total glutathione were higher in the epiDermis than in the Dermis by 513 and 471%. Thus the antioxidant capacity of the human epiDermis is far greater than that of Dermis. As the epiDermis composes the outermost 10% of the skin and acts as the initial barrier to oxidant assault, it is perhaps not surprising that it has higher levels of antioxidants.
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antioxidant defense mechanisms in murine epiDermis and Dermis and their responses to ultraviolet light
Journal of Investigative Dermatology, 1993Co-Authors: Yasuko Shindo, Eric Witt, Lester PackerAbstract:Abstract A comprehensive comparison of antioxidant defenses in the Dermis and epiDermis and their response to exposure to ultraviolet (UV) irradiation has not previously been attempted. In this study, enzymic and non-enzymic antioxidants in epiDermis and Dermis of hairless mice were compared. Enzyme activities are presented both as units/gram of skin and units/milligram of protein; arguments are presented for the superiority of skin wet weight as a reference base. Catalase, glutathione peroxidase, and glutathione reductase (units/gram of skin) were higher in epiDermis than Dermis by 49%, 86%, and 74%, respectively. Superoxide dismutase did not follow this pattern. Lipophilic antioxidants (α-tocopherol, ubiquinol 9), and ubiquinone 9 and hydrophilic antioxidants (ascorbic acid, dehydroascorbic acid, and glutathione) were 24–95% higher in epiDermis than in Dermis. In contrast, oxidized glutathione was 60% lower in epiDermis than in Dermis. Mice were irradiated with solar light to examine the response of these cutaneous layers to UV irradiation. After irradiation with 25 J/cm 2 (UVA + UVB, from a solar simulator), 10 times the minimum erythemal dose, epidermal and dermal catalase and superoxide dismutase activities were greatly decreased. α-Tocopherol, ubiquinol 9, ubiquinone 9, ascorbic acid, dehydroascorbic acid, and reduced glutathione decreased in both epiDermis and Dermis by 26–93%. Oxidized glutathione showed a slight, non-significant increase. Because the reduction in total ascorbate and catalase was much more severe in epiDermis than Dermis, it can be concluded that UV light is more damaging to the antioxidant defenses in the epiDermis than in the Dermis.