The Experts below are selected from a list of 18585 Experts worldwide ranked by ideXlab platform
Ernst Wagner - One of the best experts on this subject based on the ideXlab platform.
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toward artificial immunotoxins traceless reversible conjugation of rnase a with receptor Targeting and endosomal escape domains
Molecular Pharmaceutics, 2017Co-Authors: Xiaowen Liu, Peng Zhang, Wolfgang Rodl, Kevin Maier, Ulrich Lachelt, Ernst WagnerAbstract:The specific transport of bioactive proteins into Designated Target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-Targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-Targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A vi...
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Toward Artificial Immunotoxins: Traceless Reversible Conjugation of RNase A with Receptor Targeting and Endosomal Escape Domains
2016Co-Authors: Xiaowen Liu, Peng Zhang, Kevin Maier, Wolfgang Rödl, Ulrich Lächelt, Ernst WagnerAbstract:The specific transport of bioactive proteins into Designated Target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-Targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-Targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A via this polycation-free delivery strategy resulted in high cytotoxicity against receptor-positive KB tumor cells only when both PEG-folate and INF7 were attached
David Chichka - One of the best experts on this subject based on the ideXlab platform.
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angular declination and the dynamic perception of egocentric distance
Journal of Experimental Psychology: Human Perception and Performance, 2014Co-Authors: Daniel A Gajewski, John W Philbeck, Philip W Wirtz, David ChichkaAbstract:The extraction of the distance between an object and an observer is fast when angular declination is informative, as it is with Targets placed on the ground. To what extent does angular declination drive performance when viewing time is limited? Participants judged Target distances in a real-world environment with viewing durations ranging from 36–220 ms. An important role for angular declination was supported by experiments showing that the cue provides information about egocentric distance even on the very first glimpse, and that it supports a sensitive response to distance in the absence of other useful cues. Performance was better at 220 ms viewing durations than for briefer glimpses, suggesting that the perception of distance is dynamic even within the time frame of a typical eye fixation. Critically, performance in limited viewing trials was better when preceded by a 15 second preview of the room without a Designated Target. The results indicate that the perception of distance is powerfully shaped by memory from prior visual experience with the scene. A theoretical framework for the dynamic perception of distance is presented.
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angular declination and the dynamic perception of egocentric distance
Journal of Experimental Psychology: Human Perception and Performance, 2014Co-Authors: Daniel A Gajewski, John W Philbeck, Philip W Wirtz, David ChichkaAbstract:The extraction of the distance between an object and an observer is fast when angular declination is informative, as it is with Targets placed on the ground. To what extent does angular declination drive performance when viewing time is limited? Participants judged Target distances in a real-world environment with viewing durations ranging from 36-220 ms. An important role for angular declination was supported by experiments showing that the cue provides information about egocentric distance even on the very first glimpse, and that it supports a sensitive response to distance in the absence of other useful cues. Performance was better at 220-ms viewing durations than for briefer glimpses, suggesting that the perception of distance is dynamic even within the time frame of a typical eye fixation. Critically, performance in limited viewing trials was better when preceded by a 15-s preview of the room without a Designated Target. The results indicate that the perception of distance is powerfully shaped by memory from prior visual experience with the scene. A theoretical framework for the dynamic perception of distance is presented.
Peng Zhang - One of the best experts on this subject based on the ideXlab platform.
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toward artificial immunotoxins traceless reversible conjugation of rnase a with receptor Targeting and endosomal escape domains
Molecular Pharmaceutics, 2017Co-Authors: Xiaowen Liu, Peng Zhang, Wolfgang Rodl, Kevin Maier, Ulrich Lachelt, Ernst WagnerAbstract:The specific transport of bioactive proteins into Designated Target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-Targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-Targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A vi...
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Toward Artificial Immunotoxins: Traceless Reversible Conjugation of RNase A with Receptor Targeting and Endosomal Escape Domains
2016Co-Authors: Xiaowen Liu, Peng Zhang, Kevin Maier, Wolfgang Rödl, Ulrich Lächelt, Ernst WagnerAbstract:The specific transport of bioactive proteins into Designated Target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-Targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-Targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A via this polycation-free delivery strategy resulted in high cytotoxicity against receptor-positive KB tumor cells only when both PEG-folate and INF7 were attached
Daniel A Gajewski - One of the best experts on this subject based on the ideXlab platform.
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angular declination and the dynamic perception of egocentric distance
Journal of Experimental Psychology: Human Perception and Performance, 2014Co-Authors: Daniel A Gajewski, John W Philbeck, Philip W Wirtz, David ChichkaAbstract:The extraction of the distance between an object and an observer is fast when angular declination is informative, as it is with Targets placed on the ground. To what extent does angular declination drive performance when viewing time is limited? Participants judged Target distances in a real-world environment with viewing durations ranging from 36–220 ms. An important role for angular declination was supported by experiments showing that the cue provides information about egocentric distance even on the very first glimpse, and that it supports a sensitive response to distance in the absence of other useful cues. Performance was better at 220 ms viewing durations than for briefer glimpses, suggesting that the perception of distance is dynamic even within the time frame of a typical eye fixation. Critically, performance in limited viewing trials was better when preceded by a 15 second preview of the room without a Designated Target. The results indicate that the perception of distance is powerfully shaped by memory from prior visual experience with the scene. A theoretical framework for the dynamic perception of distance is presented.
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angular declination and the dynamic perception of egocentric distance
Journal of Experimental Psychology: Human Perception and Performance, 2014Co-Authors: Daniel A Gajewski, John W Philbeck, Philip W Wirtz, David ChichkaAbstract:The extraction of the distance between an object and an observer is fast when angular declination is informative, as it is with Targets placed on the ground. To what extent does angular declination drive performance when viewing time is limited? Participants judged Target distances in a real-world environment with viewing durations ranging from 36-220 ms. An important role for angular declination was supported by experiments showing that the cue provides information about egocentric distance even on the very first glimpse, and that it supports a sensitive response to distance in the absence of other useful cues. Performance was better at 220-ms viewing durations than for briefer glimpses, suggesting that the perception of distance is dynamic even within the time frame of a typical eye fixation. Critically, performance in limited viewing trials was better when preceded by a 15-s preview of the room without a Designated Target. The results indicate that the perception of distance is powerfully shaped by memory from prior visual experience with the scene. A theoretical framework for the dynamic perception of distance is presented.
Ulrich Lachelt - One of the best experts on this subject based on the ideXlab platform.
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toward artificial immunotoxins traceless reversible conjugation of rnase a with receptor Targeting and endosomal escape domains
Molecular Pharmaceutics, 2017Co-Authors: Xiaowen Liu, Peng Zhang, Wolfgang Rodl, Kevin Maier, Ulrich Lachelt, Ernst WagnerAbstract:The specific transport of bioactive proteins into Designated Target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy. They enter cells with receptor-Targeted specificity, respond to changing intracellular microenvironments, and by various mechanisms translocate their cytotoxic protein subunit into the cytosol. Here we imitate this toxin-based delivery strategy in an artificial setting, by bioreversible conjugation of a cytotoxic cargo protein (RNase A) with receptor-Targeting PEG-folate and the pH-specific endosomolytic peptide INF7 as synthetic delivery domains. Covalent modification of the cargo protein was achieved using the pH-labile AzMMMan linker and copper-free click chemistry with DBCO-modified delivery modules. This linkage is supposed to enable traceless intracellular release of the RNase A after exposure to the endosomal weakly acidic environment. Delivery of RNase A vi...