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Tetsuro Miyata - One of the best experts on this subject based on the ideXlab platform.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Gene Therapy, 2012Co-Authors: Hideo Kagaya, Hiroyuki Koyama, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, T Shimada, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes α_vβ_3 and α_vβ_5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA) and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation and that, PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, whereas PEG-PAsp(Det) micelles facilitated rapid, but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Nature Precedings, 2010Co-Authors: Hiroyuki Koyama, Hideo Kagaya, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes &x03B1;v&x03B2;3 and &x03B1;v&x03B2;5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA), and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation, and that PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, while PEG-PAsp(Det) micelles facilitated rapid but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.
Mathilda Boije - One of the best experts on this subject based on the ideXlab platform.
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”På sätt och vis är Det tydligt, på sätt och vis är Det inte Det” : en studie om kuratorns upplevelse av sin yrkesroll och Det psykosociala synsättet inom habiliteringen
2014Co-Authors: Mathilda BoijeAbstract:”Pa satt och vis ar Det tydligt, pa satt och vis ar Det inte Det” : en studie om kuratorns upplevelse av sin yrkesroll och Det psykosociala synsattet inom habiliteringen
Hideo Kagaya - One of the best experts on this subject based on the ideXlab platform.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Gene Therapy, 2012Co-Authors: Hideo Kagaya, Hiroyuki Koyama, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, T Shimada, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes α_vβ_3 and α_vβ_5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA) and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation and that, PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, whereas PEG-PAsp(Det) micelles facilitated rapid, but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Nature Precedings, 2010Co-Authors: Hiroyuki Koyama, Hideo Kagaya, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes &x03B1;v&x03B2;3 and &x03B1;v&x03B2;5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA), and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation, and that PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, while PEG-PAsp(Det) micelles facilitated rapid but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.
Daniel Chartier - One of the best experts on this subject based on the ideXlab platform.
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Vad är föreställningarna om Det nordliga?
2018Co-Authors: Daniel ChartierAbstract:Det nordliga har i århundraden föreställts och framställts av västerländska målare och författare. Med en gradvis ackumulering av diskursiva lager har Det med tiden lett till att Det har uppstått ”föreställningar om Det nordliga” – oavsett om Det nordliga är norra Skandinavien, Grönland, Ryssland eller Det höga Norden eller polartrakterna. I själva verket nådde västerländska upptäcktsresande Nordpolen först för hundra år sedan, vilket har medfört att ”Det nordliga” har konstruerats utifrån ett dubbelt perspektiv: ett utifrånperspektiv – konstruerat utifrån de västerländska framställningarna – och ett inifrånperspektiv – konstruerat utifrån de nordliga kulturerna (inuiter, samer, cree etc.). De förstnämnda framställningarna var ofta stereotypa och de senare ignorerade, så om vi vill studera ”Det nordliga” ur ett helhetsperspektiv måste vi ställa oss två frågor: Hur kan man definiera och förstå ”Det nordliga” genom Det föreställda? På vilka etiska principer bör en analys som siktar mot en helhetssyn vila, som särskilt inkluderar de perspektiv som tidigare har negligerats av Det sydliga? I artikeln besvarar författaren dessa två frågor, först genom att definiera föreställningarna om Det nordliga och därefter genom att föreslå ett integrativt program för att ”rekomplexifiera” Det kulturella Arktis. [Swedish]
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Hva er forestillingene om Det nordlige?
2018Co-Authors: Daniel ChartierAbstract:«Det nordlige» har gjennom århundrer blitt forestilt og fremstilt av kunstnere og forfattere fra den vestlige verden. Som følge av gradvis oppsamling av ulike diskursive lag, har med tiden «forestillinger om Det nordlige» oppstått. Dette nordlige dekker både Skandinavia, Grønland, Russland, Det høye nord og polene. Det er imidlertid bare et århundre siden de vestlige nådde Nordpolen, noe som gjør at Det «nordlige» er produktet av et dobbelt blikk, ett fra utsiden – fremstillingene, som oftest vestlige – og ett fra innsiden – de nordlige kulturene (inuittiske, skandinaviske, cree-, etc.). De første er ofte forenklede, mens de andre er miskjente. Hvis vi ønsker å studere Det «nordlige» i et helhetsperspektiv, må vi derfor stille oss to spørsmål: Hvordan kan man definere Det nordlige gjennom Det forestilte? Og hvilke etiske prinsipper må vi ta i betraktning for å kunne studere de nordlige kulturene i et helhetsperspektiv som inkluderer de som er blitt marginalisert av sørligere kulturer? I denne teksten svarer jeg på disse to spørsmålene, først ved å definere hva forestillingene om Det nordlige er, deretter ved å legge frem et inkluderende program for en mer sammensatt forståelse av Det kulturelle Arktis. Flerspråklig utgave på norsk, men også på svensk, dansk, russisk, fransk, engelsk og nordsamisk.
Hiroyuki Koyama - One of the best experts on this subject based on the ideXlab platform.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Gene Therapy, 2012Co-Authors: Hideo Kagaya, Hiroyuki Koyama, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, T Shimada, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes α_vβ_3 and α_vβ_5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA) and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation and that, PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, whereas PEG-PAsp(Det) micelles facilitated rapid, but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.
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Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions
Nature Precedings, 2010Co-Authors: Hiroyuki Koyama, Hideo Kagaya, Makoto Oba, Yutaka Miura, Takehiko Ishii, Tsuyoshi Takato, Kazunori Kataoka, Tetsuro MiyataAbstract:Gene therapy is expected to open a new strategy for the treatment of refractory vascular diseases, so the development of appropriate gene vectors for vascular lesions is needed. To realize this requirement with a non-viral approach, cyclo(RGDfK) peptide (cRGD) was introduced to block copolymer, poly(ethylene glycol)-block-polycation carrying ethylenediamine units (PEG-PAsp(Det)). cRGD recognizes &x03B1;v&x03B2;3 and &x03B1;v&x03B2;5 integrins, which are abundantly expressed in vascular lesions. cRGD-conjugated PEG-PAsp(Det) (cRGD-PEG-PAsp(Det)) formed polyplex micelles through complexation with plasmid DNA (pDNA), and the cRGD-PEG-PAsp(Det) micelles achieved significantly more efficient gene expression and cellular uptake as compared with PEG-PAsp(Det) micelles in endothelial cells and vascular smooth muscle cells. Intracellular tracking of pDNA showed that cRGD-PEG-PAsp(Det) micelles were internalized via caveolae-mediated endocytosis, which is associated with a pathway avoiding lysosomal degradation, and that PEG-PAsp(Det) micelles were transported to acidic endosomes and lysosomes via clathrin-mediated endocytosis. Further, in vivo evaluation in rat carotid artery with a neointimal lesion revealed that cRGD-PEG-PAsp(Det) micelles realized sustained gene expression, while PEG-PAsp(Det) micelles facilitated rapid but transient gene expression. These findings suggest that introduction of cRGD to polyplex micelles might create novel and useful functions for gene transfer and contribute to the establishment of efficient gene therapy for vascular diseases.