The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform

Laurent L Couetil - One of the best experts on this subject based on the ideXlab platform.

  • effect of beclomethasone dipropionate and Dexamethasone Isonicotinate on lung function bronchoalveolar lavage fluid cytology and transcription factor expression in airways of horses with recurrent airway obstruction
    Journal of Veterinary Internal Medicine, 2006
    Co-Authors: Laurent L Couetil, Brieuc De Moffarts, M Becker, Dorothee Melotte, Fabrice Jaspar, Fabrice Bureau, Pierre Lekeux
    Abstract:

    Glucocorticoid (GC) therapy is recognized to be effective for the treatment of recurrent airway obstruction (RAO) in horses. Anti-inflammatory properties of GC are thought to be mediated by suppression of inflammatory gene expression via inhibition of transcription factors such as nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1). The purpose of this study was to evaluate the effect of low-dose inhaled beclomethasone dipropionate and injectable Dexamethasone 21-Isonicotinate on clinical signs, pulmonary function, airway cytology, and activity of NF-kappaB and AP-1 in bronchial cells of RAO-affected horses. Seven horses with RAO were exposed to moldy hay until they developed airway obstruction on 3 separate occasions. In a crossover design, they were then treated with a placebo (injection on day 1), inhaled beclomethasone (500 microg q12h for 10 days), or Dexamethasone (0.06 mg/kg, IM on day 1) and monitored for 10 days. Pulmonary function, bronchoalveolar lavage fluid cytology, and NF-kappaB and AP-1 activity in bronchial brushing cells were measured before (day 1) and after treatment (day 10). Treatment with beclomethasone resulted in significantly improved pulmonary function of RAO-affected horses compared with placebo and Dexamethasone treatments. However, none of the treatments had an effect on bronchoalveolar lavage fluid cytology or NF-kappaB and AP-1 activity. These findings reveal that, in a model of severe RAO, the benefits of low-dose inhaled beclomethasone on pulmonary function are not accompanied by a decrease in airway inflammatory cells or a suppression of transcription factors NF-kappaB and AP-1 DNA-binding activity.

Pierre Lekeux - One of the best experts on this subject based on the ideXlab platform.

  • effect of beclomethasone dipropionate and Dexamethasone Isonicotinate on lung function bronchoalveolar lavage fluid cytology and transcription factor expression in airways of horses with recurrent airway obstruction
    Journal of Veterinary Internal Medicine, 2006
    Co-Authors: Laurent L Couetil, Brieuc De Moffarts, M Becker, Dorothee Melotte, Fabrice Jaspar, Fabrice Bureau, Pierre Lekeux
    Abstract:

    Glucocorticoid (GC) therapy is recognized to be effective for the treatment of recurrent airway obstruction (RAO) in horses. Anti-inflammatory properties of GC are thought to be mediated by suppression of inflammatory gene expression via inhibition of transcription factors such as nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1). The purpose of this study was to evaluate the effect of low-dose inhaled beclomethasone dipropionate and injectable Dexamethasone 21-Isonicotinate on clinical signs, pulmonary function, airway cytology, and activity of NF-kappaB and AP-1 in bronchial cells of RAO-affected horses. Seven horses with RAO were exposed to moldy hay until they developed airway obstruction on 3 separate occasions. In a crossover design, they were then treated with a placebo (injection on day 1), inhaled beclomethasone (500 microg q12h for 10 days), or Dexamethasone (0.06 mg/kg, IM on day 1) and monitored for 10 days. Pulmonary function, bronchoalveolar lavage fluid cytology, and NF-kappaB and AP-1 activity in bronchial brushing cells were measured before (day 1) and after treatment (day 10). Treatment with beclomethasone resulted in significantly improved pulmonary function of RAO-affected horses compared with placebo and Dexamethasone treatments. However, none of the treatments had an effect on bronchoalveolar lavage fluid cytology or NF-kappaB and AP-1 activity. These findings reveal that, in a model of severe RAO, the benefits of low-dose inhaled beclomethasone on pulmonary function are not accompanied by a decrease in airway inflammatory cells or a suppression of transcription factors NF-kappaB and AP-1 DNA-binding activity.

Daniel Portetelle - One of the best experts on this subject based on the ideXlab platform.

  • Influence of a hormonal preparation containing glucocorticoids (Dexamethasone esters), progestagen (chlormadinone acetate) and oestrogen (ethinyl oestradiol) on testosterone, insulin-like growth factor-1 (IGF-1), IGF-binding proteins and spermatogeni
    Animal production, 1994
    Co-Authors: Robert Renaville, Serge Massart, Georges Lognay, Anne Devolder, Myriam Sneyers, Michel Marlier, Michel Severin, Arsène Burny, Daniel Portetelle
    Abstract:

    Growth-promoters are banned by the European Community, but different hormonal cocktails are still illegally used. This experiment was therefore conducted to evaluate the effects of one of the most currently used cocktails on some hormonal parameters and spermatogenesis in finishing bulls in an attempt to provide a suitable screening technique for their illegal use. Sixteen double-muscled Belgian White Blue finishing bulls (mean ivcight: 535 (s.d. 37) kg) were blocked into control (C; no. = 7) and treated (Dex; no. = 9) groups. Animals were treated i.m. with the hormonal preparation (Dexamethasone Isonicotinate and phosphate, chlormadinone acetate and ethinyl oestradiol) on day 0, day 15 and day 30. Animals were slaughtered on day 45. Three h before each treatment and just prior to slaughter, jugular blood samples were collected to monitor the testosterone (T) response to an i.v. injection of gonadotropin releasing hormone (GnRH) (0·5 fig GnRH per kg body weight). Testicular tissue was also collected at slaughter. Plasma T and insulin-like growth factor-1 (IGF-1) concentrations were measured by radioimmunoassay. IGF-binding proteins (IGFBPs) were evaluated using Western ligand blotting. Daily weight gains were lower in the control group (1·29 (s.d. 0·13) kg for C v. 1·60 (s.d. 0·39) kg for Dex) but the difference ivas not significant. After treatment, spermatogonia, spermatocytes, spermatids and spermatozoa disappeared from the testis and seminiferous tubules consisted only of Sertoli cells; these observations suggest that treated animals were sterile. Moreover, plasma T concentrations in response to GnRH stimulation were suppressed f P v.

  • Influence of a hormonal preparation containing glucocorticoids (Dexamethasone esters), progestagen (chlormadinone acetate) and oestrogen (ethinyl oestradiol) on testosterone, insulin-like growth factor-1 (IGF-1), IGF-binding proteins and spermatogeni
    Animal Science, 1994
    Co-Authors: Robert Renaville, Serge Massart, Georges Lognay, Anne Devolder, Myriam Sneyers, Michel Marlier, Michel Severin, Arsène Burny, Daniel Portetelle
    Abstract:

    AbstractGrowth-promoters are banned by the European Community, but different hormonal cocktails are still illegally used. This experiment was therefore conducted to evaluate the effects of one of the most currently used cocktails on some hormonal parameters and spermatogenesis in finishing bulls in an attempt to provide a suitable screening technique for their illegal use. Sixteen double-muscled Belgian White Blue finishing bulls (mean ivcight: 535 (s.d. 37) kg) were blocked into control (C; no. = 7) and treated (Dex; no. = 9) groups. Animals were treated i.m. with the hormonal preparation (Dexamethasone Isonicotinate and phosphate, chlormadinone acetate and ethinyl oestradiol) on day 0, day 15 and day 30. Animals were slaughtered on day 45. Three h before each treatment and just prior to slaughter, jugular blood samples were collected to monitor the testosterone (T) response to an i.v. injection of gonadotropin releasing hormone (GnRH) (0·5 fig GnRH per kg body weight). Testicular tissue was also collected at slaughter. Plasma T and insulin-like growth factor-1 (IGF-1) concentrations were measured by radioimmunoassay. IGF-binding proteins (IGFBPs) were evaluated using Western ligand blotting. Daily weight gains were lower in the control group (1·29 (s.d. 0·13) kg for C v. 1·60 (s.d. 0·39) kg for Dex) but the difference ivas not significant. After treatment, spermatogonia, spermatocytes, spermatids and spermatozoa disappeared from the testis and seminiferous tubules consisted only of Sertoli cells; these observations suggest that treated animals were sterile. Moreover, plasma T concentrations in response to GnRH stimulation were suppressed fP < 0·001) in the Dex group between day 15 and day 45 (mean maximal responses: 5·4 to 7·9 μg/l in C group v. < 0·2 μg/l in Dex group at day 15, 30 and 45). Treatment did not show any prominent effect on plasma IGF-1 levels but increased IGFBPS band intensity. In conclusion, treatment with a cocktail containing Dexamethasone esters, chlormadinone acetate and ethinyl oestradiol for a short period induced a number of changes in finishing bulls luhich might be possible to develop as a screening method for the identification of illegally treated animals.

Robert Renaville - One of the best experts on this subject based on the ideXlab platform.

  • Influence of a hormonal preparation containing glucocorticoids (Dexamethasone esters), progestagen (chlormadinone acetate) and oestrogen (ethinyl oestradiol) on testosterone, insulin-like growth factor-1 (IGF-1), IGF-binding proteins and spermatogeni
    Animal production, 1994
    Co-Authors: Robert Renaville, Serge Massart, Georges Lognay, Anne Devolder, Myriam Sneyers, Michel Marlier, Michel Severin, Arsène Burny, Daniel Portetelle
    Abstract:

    Growth-promoters are banned by the European Community, but different hormonal cocktails are still illegally used. This experiment was therefore conducted to evaluate the effects of one of the most currently used cocktails on some hormonal parameters and spermatogenesis in finishing bulls in an attempt to provide a suitable screening technique for their illegal use. Sixteen double-muscled Belgian White Blue finishing bulls (mean ivcight: 535 (s.d. 37) kg) were blocked into control (C; no. = 7) and treated (Dex; no. = 9) groups. Animals were treated i.m. with the hormonal preparation (Dexamethasone Isonicotinate and phosphate, chlormadinone acetate and ethinyl oestradiol) on day 0, day 15 and day 30. Animals were slaughtered on day 45. Three h before each treatment and just prior to slaughter, jugular blood samples were collected to monitor the testosterone (T) response to an i.v. injection of gonadotropin releasing hormone (GnRH) (0·5 fig GnRH per kg body weight). Testicular tissue was also collected at slaughter. Plasma T and insulin-like growth factor-1 (IGF-1) concentrations were measured by radioimmunoassay. IGF-binding proteins (IGFBPs) were evaluated using Western ligand blotting. Daily weight gains were lower in the control group (1·29 (s.d. 0·13) kg for C v. 1·60 (s.d. 0·39) kg for Dex) but the difference ivas not significant. After treatment, spermatogonia, spermatocytes, spermatids and spermatozoa disappeared from the testis and seminiferous tubules consisted only of Sertoli cells; these observations suggest that treated animals were sterile. Moreover, plasma T concentrations in response to GnRH stimulation were suppressed f P v.

  • Influence of a hormonal preparation containing glucocorticoids (Dexamethasone esters), progestagen (chlormadinone acetate) and oestrogen (ethinyl oestradiol) on testosterone, insulin-like growth factor-1 (IGF-1), IGF-binding proteins and spermatogeni
    Animal Science, 1994
    Co-Authors: Robert Renaville, Serge Massart, Georges Lognay, Anne Devolder, Myriam Sneyers, Michel Marlier, Michel Severin, Arsène Burny, Daniel Portetelle
    Abstract:

    AbstractGrowth-promoters are banned by the European Community, but different hormonal cocktails are still illegally used. This experiment was therefore conducted to evaluate the effects of one of the most currently used cocktails on some hormonal parameters and spermatogenesis in finishing bulls in an attempt to provide a suitable screening technique for their illegal use. Sixteen double-muscled Belgian White Blue finishing bulls (mean ivcight: 535 (s.d. 37) kg) were blocked into control (C; no. = 7) and treated (Dex; no. = 9) groups. Animals were treated i.m. with the hormonal preparation (Dexamethasone Isonicotinate and phosphate, chlormadinone acetate and ethinyl oestradiol) on day 0, day 15 and day 30. Animals were slaughtered on day 45. Three h before each treatment and just prior to slaughter, jugular blood samples were collected to monitor the testosterone (T) response to an i.v. injection of gonadotropin releasing hormone (GnRH) (0·5 fig GnRH per kg body weight). Testicular tissue was also collected at slaughter. Plasma T and insulin-like growth factor-1 (IGF-1) concentrations were measured by radioimmunoassay. IGF-binding proteins (IGFBPs) were evaluated using Western ligand blotting. Daily weight gains were lower in the control group (1·29 (s.d. 0·13) kg for C v. 1·60 (s.d. 0·39) kg for Dex) but the difference ivas not significant. After treatment, spermatogonia, spermatocytes, spermatids and spermatozoa disappeared from the testis and seminiferous tubules consisted only of Sertoli cells; these observations suggest that treated animals were sterile. Moreover, plasma T concentrations in response to GnRH stimulation were suppressed fP < 0·001) in the Dex group between day 15 and day 45 (mean maximal responses: 5·4 to 7·9 μg/l in C group v. < 0·2 μg/l in Dex group at day 15, 30 and 45). Treatment did not show any prominent effect on plasma IGF-1 levels but increased IGFBPS band intensity. In conclusion, treatment with a cocktail containing Dexamethasone esters, chlormadinone acetate and ethinyl oestradiol for a short period induced a number of changes in finishing bulls luhich might be possible to develop as a screening method for the identification of illegally treated animals.

Fabrice Bureau - One of the best experts on this subject based on the ideXlab platform.

  • effect of beclomethasone dipropionate and Dexamethasone Isonicotinate on lung function bronchoalveolar lavage fluid cytology and transcription factor expression in airways of horses with recurrent airway obstruction
    Journal of Veterinary Internal Medicine, 2006
    Co-Authors: Laurent L Couetil, Brieuc De Moffarts, M Becker, Dorothee Melotte, Fabrice Jaspar, Fabrice Bureau, Pierre Lekeux
    Abstract:

    Glucocorticoid (GC) therapy is recognized to be effective for the treatment of recurrent airway obstruction (RAO) in horses. Anti-inflammatory properties of GC are thought to be mediated by suppression of inflammatory gene expression via inhibition of transcription factors such as nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1). The purpose of this study was to evaluate the effect of low-dose inhaled beclomethasone dipropionate and injectable Dexamethasone 21-Isonicotinate on clinical signs, pulmonary function, airway cytology, and activity of NF-kappaB and AP-1 in bronchial cells of RAO-affected horses. Seven horses with RAO were exposed to moldy hay until they developed airway obstruction on 3 separate occasions. In a crossover design, they were then treated with a placebo (injection on day 1), inhaled beclomethasone (500 microg q12h for 10 days), or Dexamethasone (0.06 mg/kg, IM on day 1) and monitored for 10 days. Pulmonary function, bronchoalveolar lavage fluid cytology, and NF-kappaB and AP-1 activity in bronchial brushing cells were measured before (day 1) and after treatment (day 10). Treatment with beclomethasone resulted in significantly improved pulmonary function of RAO-affected horses compared with placebo and Dexamethasone treatments. However, none of the treatments had an effect on bronchoalveolar lavage fluid cytology or NF-kappaB and AP-1 activity. These findings reveal that, in a model of severe RAO, the benefits of low-dose inhaled beclomethasone on pulmonary function are not accompanied by a decrease in airway inflammatory cells or a suppression of transcription factors NF-kappaB and AP-1 DNA-binding activity.