The Experts below are selected from a list of 801 Experts worldwide ranked by ideXlab platform
Díaz Tirado, Víctor Augusto - One of the best experts on this subject based on the ideXlab platform.
-
Ruptura de enlaces α (1,6) de la dextrana mediante la enzima dextranaza para la obtención de moléculas de glucosa y su aprovechamiento posterior en etanol en la empresa Casa Grande S.A.A.
Universidad Nacional de Trujillo, 2017Co-Authors: Loyola Moreno, Lucy Giovanna, Díaz Tirado, Víctor AugustoAbstract:El propósito de esta tesis es dar solución al problema generado en la Empresa Azucarera CASAGRANDE S.A.A referente a la dextrana presente en la caña de azúcar y durante todo el proceso industrial de obtención del azúcar. Esto significa conseguir un mayor rendimiento en la producción de azúcar y por consiguiente aumentar las ganancias económicas de la empresa en mención. Dicho problema suscitado se ha resuelto mediante el empleo de la enzima Dextranasa con la finalidad de romper los enlaces α (1,6) de la Dextrana, recuperando la glucosa de la sacarosa polimerizada por la Dextrana y aprovechándola para obtener etanol. Todo ello planteó el reto de poner en práctica una metodología eficiente basada en la dosificación adecuada y eficaz de la enzima Dextranasa a 10, 15, 25, 30, 60 ppm y a tiempos de 10, 20 y 30 minutos en los jarabes de tipo A, B y C. De esta manera, se consiguió bajar los niveles de Dextrana y se recuperó la mayor cantidad de glucosa para la obtención de etanol, teniendo como eficiencia óptima la dosificación de 15 ppm de enzima Dextranasa y a un tiempo de 20 minutos en la aplicación de los jarabes. Por lo tanto, esta tesis está dirigida a disminuir los niveles de dextrana originados desde cosecha de la caña de azúcar, la cual es perjudicial porque aumenta la viscosidad en los jugos y consume la sacarosa de manera irreversible. Consiguiendo esta disminución se consigue aumentar la producción de azúcar, además de obtener etanol a partir de la glucosa recuperada en la sacarosa. Y por último se consigue impulsar el desarrollo sostenible de la empresa orientado a su crecimiento económico mediante la generación de mayores ingresos económicosTesisThe purpose of this thesis is to solve the problem generated in the Sugar Company CASAGRANDE S.A.A referring to the dextran present in the sugar cane and throughout the industrial process of obtaining the sugar. This means achieving a higher return on sugar production and therefore increasing the economic profit of the company in question. This problem has been solved by the use of the enzyme Dextranase in order to break the α (1,6) bonds of Dextran, recovering glucose from the sucrose polymerized by Dextran and taking advantage of it to obtain ethanol. All this raised the challenge of putting into practice an efficient methodology based on the adequate and effective dosing of the enzyme Dextranase at 10, 15, 25, 30, 60 ppm and times of 10, 20 and 30 minutes in syrup type A, B and C. In this way, the levels of Dextran were lowered and the highest amount of glucose was recovered to obtain ethanol, with the optimum efficiency being the dosage of 15 ppm Dextranase enzyme and a time of 20 minutes in the application of syrups. Therefore, this thesis is aimed at reducing the levels of dextran originating from sugarcane harvesting, which is detrimental because it increases the viscosity in the juices and consumes the sucrose irreversibly. Achieving this decrease is achieved increase sugar production, in addition to obtaining ethanol from the glucose recovered in sucrose. And lastly, it is possible to promote the sustainable development of the company aimed at its economic growth by generating higher economic income
-
Ruptura de enlaces α (1,6) de la dextrana mediante la enzima dextranaza para la obtención de moléculas de glucosa y su aprovechamiento posterior en etanol en la empresa casa grande s.a.a.
Universidad Nacional de Trujillo, 2016Co-Authors: Loyola Moreno, Lucy Giovanna, Díaz Tirado, Víctor AugustoAbstract:El propósito de esta tesis es dar solución al problema generado en la Empresa Azucarera CASAGRANDE S.A.A referente a la dextrana presente en la caña de azúcar y durante todo el proceso industrial de obtención del azúcar. Esto significa conseguir un mayor rendimiento en la producción de azúcar y por consiguiente aumentar las ganancias económicas de la empresa en mención. Dicho problema suscitado se ha resuelto mediante el empleo de la enzima Dextranasa con la finalidad de romper los enlaces α (1,6) de la Dextrana, recuperando la glucosa de la sacarosa polimerizada por la Dextrana y aprovechándola para obtener etanol. Todo ello planteó el reto de poner en práctica una metodología eficiente basada en la dosificación adecuada y eficaz de la enzima Dextranasa a 10, 15, 25, 30, 60 ppm y a tiempos de 10, 20 y 30 minutos en los jarabes de tipo A, B y C. De esta manera, se consiguió bajar los niveles de Dextrana y se recuperó la mayor cantidad de glucosa para la obtención de etanol, teniendo como eficiencia óptima la dosificación de 15 ppm de enzima Dextranasa y a un tiempo de 20 minutos en la aplicación de los jarabes. Por lo tanto, esta tesis está dirigida a disminuir los niveles de dextrana originados desde cosecha de la caña de azúcar, la cual es perjudicial porque aumenta la viscosidad en los jugos y consume la sacarosa de manera irreversible. Consiguiendo esta disminución se consigue aumentar la producción de azúcar, además de obtener etanol a partir de la glucosa recuperada en la sacarosa. Y por último se consigue impulsar el desarrollo sostenible de la empresa orientado a su crecimiento económico mediante la generación de mayores ingresos económicos.The purpose of this thesis is to solve the problem generated in the Company Azucarera CASAGRANDE S.A.A concerning the dextran present in sugar cane and throughout the industrial process of obtaining sugar. This means getting a higher yield in sugar production and consequently increase economic profits of the company in question. Said raised problem is solved by using Dextranase enzyme in order to break the links α (1,6) of Dextran, recovering polymerized glucose and sucrose Dextran by taking advantage of it to obtain ethanol. All this raised the challenge of implementing an efficient methodology based on the adequate and effective dosage of Dextranase enzyme to 10, 15, 25, 30, 60 ppm and times of 10, 20 and 30 minutes syrups type A, B and C. Thus was achieved lower levels of dextran and more glucose for obtaining ethanol was recovered, having as optimal efficiency dosing Dextranase enzyme 15 ppm and a time of 20 minutes in the application syrups. Therefore, this thesis is aimed at decreasing levels of dextran originated from harvest sugar cane, which is harmful because it increases the viscosity juices and consume sucrose irreversibly. Getting this decline is able to increase sugar production, in addition to obtaining ethanol from glucose recovered in sucrose. And finally it gets boost sustainable development of the company oriented economic growth by generating more income
Shuichi Takayama - One of the best experts on this subject based on the ideXlab platform.
-
Membraneless Compartmentalization Facilitates Enzymatic Cascade Reactions and Reduces Substrate Inhibition
2018Co-Authors: Taisuke Kojima, Shuichi TakayamaAbstract:Living cells possess membraneless organelles formed by liquid–liquid phase separation. With the aim of better understanding the general functions of membraneless microcompartments, this paper constructs acellular multicompartment reaction systems using an aqueous multiphase system. Membraneless coacervate droplets are placed within a molecularly crowded environment, where a larger dextran (DEX) droplet is submerged in a polyethylene glycol (PEG) solution. The coacervate droplets are capable of sequestering reagents and enzymes with a long retention time, and demonstrate multistep cascading reactions through the liquid–liquid interfaces. The ability to change phase dynamics is also demonstrated through salt-mediated dissolution of coacervate droplets, which leads to the release and mixing of separately sequestered reagents and enzymes. Finally, as phase-separated materials in membraneless organelles are often substrates and substrate analogues for the enzymes sequestered or excluded in the organelles, this paper explores the interaction between DEX and Dextranase, an enzyme that hydrolyzes DEX. The results reveal that Dextranase suffers from substrate inhibition when partitioned directly in a DEX phase but that this inhibition can be mitigated and reactions greatly accelerated by compartmentalization of Dextranase inside a coacervate droplet that is adjacent to, but phase-separated from, the DEX phase. The insight that compartmentalization of enzymes can accelerate reactions by mitigating substrate inhibition is particularly novel and is an example where artificial membraneless organelle-like systems may provide new insights into physiological cell functions
-
Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions
2016Co-Authors: David Lai, John P Frampton, Michael Tsuei, Albert Kao, Shuichi TakayamaAbstract:ABSTRACT: Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity. Assays for measuring the degradation of dextran (DEX)either require the use of specially prepared labeled polymers as surrogate substrates1 or indirect measurement of degradation products, such as the amount of reducing suga
-
Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions
2015Co-Authors: David Lai, John P Frampton, Michael Tsuei, Albert Kao, Shuichi TakayamaAbstract:Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity
-
label free direct visual analysis of hydrolytic enzyme activity using aqueous two phase system droplet phase transitions
Analytical Chemistry, 2014Co-Authors: John P Frampton, Michael Tsuei, Shuichi TakayamaAbstract:Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity.
Loyola Moreno, Lucy Giovanna - One of the best experts on this subject based on the ideXlab platform.
-
Ruptura de enlaces α (1,6) de la dextrana mediante la enzima dextranaza para la obtención de moléculas de glucosa y su aprovechamiento posterior en etanol en la empresa Casa Grande S.A.A.
Universidad Nacional de Trujillo, 2017Co-Authors: Loyola Moreno, Lucy Giovanna, Díaz Tirado, Víctor AugustoAbstract:El propósito de esta tesis es dar solución al problema generado en la Empresa Azucarera CASAGRANDE S.A.A referente a la dextrana presente en la caña de azúcar y durante todo el proceso industrial de obtención del azúcar. Esto significa conseguir un mayor rendimiento en la producción de azúcar y por consiguiente aumentar las ganancias económicas de la empresa en mención. Dicho problema suscitado se ha resuelto mediante el empleo de la enzima Dextranasa con la finalidad de romper los enlaces α (1,6) de la Dextrana, recuperando la glucosa de la sacarosa polimerizada por la Dextrana y aprovechándola para obtener etanol. Todo ello planteó el reto de poner en práctica una metodología eficiente basada en la dosificación adecuada y eficaz de la enzima Dextranasa a 10, 15, 25, 30, 60 ppm y a tiempos de 10, 20 y 30 minutos en los jarabes de tipo A, B y C. De esta manera, se consiguió bajar los niveles de Dextrana y se recuperó la mayor cantidad de glucosa para la obtención de etanol, teniendo como eficiencia óptima la dosificación de 15 ppm de enzima Dextranasa y a un tiempo de 20 minutos en la aplicación de los jarabes. Por lo tanto, esta tesis está dirigida a disminuir los niveles de dextrana originados desde cosecha de la caña de azúcar, la cual es perjudicial porque aumenta la viscosidad en los jugos y consume la sacarosa de manera irreversible. Consiguiendo esta disminución se consigue aumentar la producción de azúcar, además de obtener etanol a partir de la glucosa recuperada en la sacarosa. Y por último se consigue impulsar el desarrollo sostenible de la empresa orientado a su crecimiento económico mediante la generación de mayores ingresos económicosTesisThe purpose of this thesis is to solve the problem generated in the Sugar Company CASAGRANDE S.A.A referring to the dextran present in the sugar cane and throughout the industrial process of obtaining the sugar. This means achieving a higher return on sugar production and therefore increasing the economic profit of the company in question. This problem has been solved by the use of the enzyme Dextranase in order to break the α (1,6) bonds of Dextran, recovering glucose from the sucrose polymerized by Dextran and taking advantage of it to obtain ethanol. All this raised the challenge of putting into practice an efficient methodology based on the adequate and effective dosing of the enzyme Dextranase at 10, 15, 25, 30, 60 ppm and times of 10, 20 and 30 minutes in syrup type A, B and C. In this way, the levels of Dextran were lowered and the highest amount of glucose was recovered to obtain ethanol, with the optimum efficiency being the dosage of 15 ppm Dextranase enzyme and a time of 20 minutes in the application of syrups. Therefore, this thesis is aimed at reducing the levels of dextran originating from sugarcane harvesting, which is detrimental because it increases the viscosity in the juices and consumes the sucrose irreversibly. Achieving this decrease is achieved increase sugar production, in addition to obtaining ethanol from the glucose recovered in sucrose. And lastly, it is possible to promote the sustainable development of the company aimed at its economic growth by generating higher economic income
-
Ruptura de enlaces α (1,6) de la dextrana mediante la enzima dextranaza para la obtención de moléculas de glucosa y su aprovechamiento posterior en etanol en la empresa casa grande s.a.a.
Universidad Nacional de Trujillo, 2016Co-Authors: Loyola Moreno, Lucy Giovanna, Díaz Tirado, Víctor AugustoAbstract:El propósito de esta tesis es dar solución al problema generado en la Empresa Azucarera CASAGRANDE S.A.A referente a la dextrana presente en la caña de azúcar y durante todo el proceso industrial de obtención del azúcar. Esto significa conseguir un mayor rendimiento en la producción de azúcar y por consiguiente aumentar las ganancias económicas de la empresa en mención. Dicho problema suscitado se ha resuelto mediante el empleo de la enzima Dextranasa con la finalidad de romper los enlaces α (1,6) de la Dextrana, recuperando la glucosa de la sacarosa polimerizada por la Dextrana y aprovechándola para obtener etanol. Todo ello planteó el reto de poner en práctica una metodología eficiente basada en la dosificación adecuada y eficaz de la enzima Dextranasa a 10, 15, 25, 30, 60 ppm y a tiempos de 10, 20 y 30 minutos en los jarabes de tipo A, B y C. De esta manera, se consiguió bajar los niveles de Dextrana y se recuperó la mayor cantidad de glucosa para la obtención de etanol, teniendo como eficiencia óptima la dosificación de 15 ppm de enzima Dextranasa y a un tiempo de 20 minutos en la aplicación de los jarabes. Por lo tanto, esta tesis está dirigida a disminuir los niveles de dextrana originados desde cosecha de la caña de azúcar, la cual es perjudicial porque aumenta la viscosidad en los jugos y consume la sacarosa de manera irreversible. Consiguiendo esta disminución se consigue aumentar la producción de azúcar, además de obtener etanol a partir de la glucosa recuperada en la sacarosa. Y por último se consigue impulsar el desarrollo sostenible de la empresa orientado a su crecimiento económico mediante la generación de mayores ingresos económicos.The purpose of this thesis is to solve the problem generated in the Company Azucarera CASAGRANDE S.A.A concerning the dextran present in sugar cane and throughout the industrial process of obtaining sugar. This means getting a higher yield in sugar production and consequently increase economic profits of the company in question. Said raised problem is solved by using Dextranase enzyme in order to break the links α (1,6) of Dextran, recovering polymerized glucose and sucrose Dextran by taking advantage of it to obtain ethanol. All this raised the challenge of implementing an efficient methodology based on the adequate and effective dosage of Dextranase enzyme to 10, 15, 25, 30, 60 ppm and times of 10, 20 and 30 minutes syrups type A, B and C. Thus was achieved lower levels of dextran and more glucose for obtaining ethanol was recovered, having as optimal efficiency dosing Dextranase enzyme 15 ppm and a time of 20 minutes in the application syrups. Therefore, this thesis is aimed at decreasing levels of dextran originated from harvest sugar cane, which is harmful because it increases the viscosity juices and consume sucrose irreversibly. Getting this decline is able to increase sugar production, in addition to obtaining ethanol from glucose recovered in sucrose. And finally it gets boost sustainable development of the company oriented economic growth by generating more income
John P Frampton - One of the best experts on this subject based on the ideXlab platform.
-
Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions
2016Co-Authors: David Lai, John P Frampton, Michael Tsuei, Albert Kao, Shuichi TakayamaAbstract:ABSTRACT: Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity. Assays for measuring the degradation of dextran (DEX)either require the use of specially prepared labeled polymers as surrogate substrates1 or indirect measurement of degradation products, such as the amount of reducing suga
-
Label-Free Direct Visual Analysis of Hydrolytic Enzyme Activity Using Aqueous Two-Phase System Droplet Phase Transitions
2015Co-Authors: David Lai, John P Frampton, Michael Tsuei, Albert Kao, Shuichi TakayamaAbstract:Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity
-
label free direct visual analysis of hydrolytic enzyme activity using aqueous two phase system droplet phase transitions
Analytical Chemistry, 2014Co-Authors: John P Frampton, Michael Tsuei, Shuichi TakayamaAbstract:Dextran hydrolysis-mediated conversion of polyethylene glycol (PEG)-dextran (DEX) aqueous two-phase system droplets to a single phase was used to directly visualize Dextranase activity. DEX droplets were formed either by manual micropipetting or within a continuous PEG phase by computer controlled actuation of an orifice connecting rounded channels formed by backside diffused light lithography. The time required for the two-phase to one-phase transition was dependent on the Dextranase concentration, pH of the medium, and temperature. The apparent Michaelis constants for Dextranase were estimated based on previously reported catalytic constants, the binodal polymer concentration curves for PEG-DEX phase transition for each temperature, and pH condition. The combination of a microfluidic droplet system and phase transition observation provides a new method for label-free direct measurement of enzyme activity.
Peter L. Bergquist - One of the best experts on this subject based on the ideXlab platform.
-
A Paenibacillus sp. Dextranase mutant pool with improved thermostability and activity
Applied Microbiology and Biotechnology, 2007Co-Authors: Erika Hild, Stevens M. Brumbley, Michael G. O’shea, Helena Nevalainen, Peter L. BergquistAbstract:Random mutagenesis was used to create a library of chimeric Dextranase ( dex1 ) genes. A plate-screening protocol was developed with improved thermostability as a selection criterion. The mutant library was screened for active Dextranase variants by observing clearing zones on dextran-blue agar plates at 50°C after exposure to 68°C for 2 h, a temperature regime at which wild-type activity was abolished. A number of potentially improved variants were identified by this strategy, five of which were further characterised. DNA sequencing revealed ten nucleotide substitutions, ranging from one to four per variant. Thermal inactivation studies showed reduced (2.9-fold) thermostability for one variant and similar thermostability for a second variant, but confirmed improved thermostability for three mutants with 2.3- (28.9 min) to 6.9-fold (86.6 min) increases in half-lives at 62°C compared to that of the wild-type enzyme (12.6 min). Using a 10-min assay, apparent temperature optima of the variants were similar to that of the wild type ( T _opt 60°C). However, one of these variants had increased enzyme activity. Therefore, the first-generation Dextranase mutant pool obtained in this study has sufficient molecular diversity for further improvements in both thermostability and activity through recombination (gene shuffling).