The Experts below are selected from a list of 165 Experts worldwide ranked by ideXlab platform

Pierre Abad - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of a satellite DNA from Meloidogyne hapla and its use as a Diagnostic Probe
    Phytopathology, 1995
    Co-Authors: Christine Piotte, Philippe Castagnone-sereno, M. Bongiovanni, A. Dalmasso, Pierre Abad
    Abstract:

    A Styl satellite DNA previously isolated from Meloidogyne hapla gave hybridization signals only with the five M. hapla populations tested in Southern blot experiments, indicating that this satellite sequence is species-specific. Because of its high level of reiteration and its variability, this sequence was able to discriminate both between Meloidogyne species and among three M. hapla populations. The StyI satellite DNA was polymorphic at the intraspecific level, since hybridization patterns of M. hapla populations exhibited very different profiles with polymorphisms characterizing each of the three populations tested. In simple squashed-nematode experiments, we were able to unambiguously identify M. hapla vs. M. chitwoodi, which are sympatric. The procedure was effective even on a single female located in root tissues, with the main advantage that it avoided time-consuming DNA extraction procedures. Therefore, in a case where confusion may occur among some particularly damaging nematodes, this very sensitive technique may prove to be reliable. Such satellite DNA sequences should provide rapid, inexpensive, and user-friendly field tools for Meloidogyne species identification

  • Use of species-specific satellite DNA from Bursaphelenchus xylophilus as a Diagnostic Probe
    Phytopathology, 1994
    Co-Authors: Sophie Tares, J.-m. Lemontey, G. De Guiran, Pierre Abad
    Abstract:

    We describe the development of a species-specific DNA Probe with the satellite DNA (satDNA) isolated from the Japanese J10 isolate of Bursaphelenchus xylophilus. We show that this MspI satDNA Probe is effective directly on a single squashed nematode spotted onto a filter. Therefore, in the pinewood nematode species complex, the identification of the plant pathogenic species B. xylophilus may be accomplished both rapidly and reliably. We also show that the MspI satellite sequence is polymorphic within B. xylophilus species. Hybridization patterns of B. xylophilus isolates with the MspI satellite Probe result in very different profiles, with polymorphisms characterizing each of the tested strains [...]

Hans Christian Wulf - One of the best experts on this subject based on the ideXlab platform.

  • NIR-FT Raman spectroscopy as a Diagnostic Probe for mummified skin and nails
    Vibrational Spectroscopy, 2002
    Co-Authors: Howell G.m. Edwards, M. Gniadecka, Susanne Germann Petersen, Jens Hansen, Ole Faurskov Nielsen, Daniel H. Christensen, Hans Christian Wulf
    Abstract:

    Abstract Raman spectroscopy is able to provide novel information non-destructively in the analysis of ancient skin tissue samples. The technique is well-suited to partially desiccated or wet samples of skin, which may have been subjected to varying degrees of degradation, depending on the burial environment. Suitable selection of wavelength of excitation and use of a Raman microscope offer a means of accessing information from small samples, which may be required for study afterwards using destructive analytical methods. From comparisons with the Raman spectra of healthy and diseased contemporary skin specimens, it is possible to discover whether the skin preservation has been natural or assisted by chemicals used in mummification processes. Such information is of importance in archaeological conservation and can shed light on historical practices. In this study will be presented the results from Raman spectroscopic analysis of the skin of the 5200-year-old “ice-man” of the Alps and, skin and nail samples from the mediaeval Qilakitsoq ice-mummies (500-year-old) and skin from the hot-desert Chiribaya Mummies (1000-year-old).

Howell G.m. Edwards - One of the best experts on this subject based on the ideXlab platform.

  • NIR-FT Raman spectroscopy as a Diagnostic Probe for mummified skin and nails
    Vibrational Spectroscopy, 2002
    Co-Authors: Howell G.m. Edwards, M. Gniadecka, Susanne Germann Petersen, Jens Hansen, Ole Faurskov Nielsen, Daniel H. Christensen, Hans Christian Wulf
    Abstract:

    Abstract Raman spectroscopy is able to provide novel information non-destructively in the analysis of ancient skin tissue samples. The technique is well-suited to partially desiccated or wet samples of skin, which may have been subjected to varying degrees of degradation, depending on the burial environment. Suitable selection of wavelength of excitation and use of a Raman microscope offer a means of accessing information from small samples, which may be required for study afterwards using destructive analytical methods. From comparisons with the Raman spectra of healthy and diseased contemporary skin specimens, it is possible to discover whether the skin preservation has been natural or assisted by chemicals used in mummification processes. Such information is of importance in archaeological conservation and can shed light on historical practices. In this study will be presented the results from Raman spectroscopic analysis of the skin of the 5200-year-old “ice-man” of the Alps and, skin and nail samples from the mediaeval Qilakitsoq ice-mummies (500-year-old) and skin from the hot-desert Chiribaya Mummies (1000-year-old).

  • Raman spectroscopy as a Diagnostic Probe for ancient skin
    Biomedical Applications of Raman Spectroscopy, 1999
    Co-Authors: Howell G.m. Edwards
    Abstract:

    We discuss the use of Raman microProbe spectroscopy and Raman imaging to study the chemical composition of fresh, unmounted bone at a microscopic level. A specimen of human cortical bone was analyzed and evidence for the presence of amorphous-type calcium phosphate, a theoretical precursor in the bone formation process, was found. In general the amorphous-type calcium phosphate appears away from osteons, in the interstitial tissue. This finding calls into question the role of amorphous-type calcium phosphate as a precursor to apatitic phosphate, since it was not found in the recently remodeled bone near the osteon center, but rather in older bone tissue. Some reasons for the presence of amorphous calcium phosphate are proposed. Possible relations of the amorphous mineral to bone damage and bone remodeling are discussed.

Susanne Germann Petersen - One of the best experts on this subject based on the ideXlab platform.

  • NIR-FT Raman spectroscopy as a Diagnostic Probe for mummified skin and nails
    Vibrational Spectroscopy, 2002
    Co-Authors: Howell G.m. Edwards, M. Gniadecka, Susanne Germann Petersen, Jens Hansen, Ole Faurskov Nielsen, Daniel H. Christensen, Hans Christian Wulf
    Abstract:

    Abstract Raman spectroscopy is able to provide novel information non-destructively in the analysis of ancient skin tissue samples. The technique is well-suited to partially desiccated or wet samples of skin, which may have been subjected to varying degrees of degradation, depending on the burial environment. Suitable selection of wavelength of excitation and use of a Raman microscope offer a means of accessing information from small samples, which may be required for study afterwards using destructive analytical methods. From comparisons with the Raman spectra of healthy and diseased contemporary skin specimens, it is possible to discover whether the skin preservation has been natural or assisted by chemicals used in mummification processes. Such information is of importance in archaeological conservation and can shed light on historical practices. In this study will be presented the results from Raman spectroscopic analysis of the skin of the 5200-year-old “ice-man” of the Alps and, skin and nail samples from the mediaeval Qilakitsoq ice-mummies (500-year-old) and skin from the hot-desert Chiribaya Mummies (1000-year-old).

Jens Hansen - One of the best experts on this subject based on the ideXlab platform.

  • NIR-FT Raman spectroscopy as a Diagnostic Probe for mummified skin and nails
    Vibrational Spectroscopy, 2002
    Co-Authors: Howell G.m. Edwards, M. Gniadecka, Susanne Germann Petersen, Jens Hansen, Ole Faurskov Nielsen, Daniel H. Christensen, Hans Christian Wulf
    Abstract:

    Abstract Raman spectroscopy is able to provide novel information non-destructively in the analysis of ancient skin tissue samples. The technique is well-suited to partially desiccated or wet samples of skin, which may have been subjected to varying degrees of degradation, depending on the burial environment. Suitable selection of wavelength of excitation and use of a Raman microscope offer a means of accessing information from small samples, which may be required for study afterwards using destructive analytical methods. From comparisons with the Raman spectra of healthy and diseased contemporary skin specimens, it is possible to discover whether the skin preservation has been natural or assisted by chemicals used in mummification processes. Such information is of importance in archaeological conservation and can shed light on historical practices. In this study will be presented the results from Raman spectroscopic analysis of the skin of the 5200-year-old “ice-man” of the Alps and, skin and nail samples from the mediaeval Qilakitsoq ice-mummies (500-year-old) and skin from the hot-desert Chiribaya Mummies (1000-year-old).