The Experts below are selected from a list of 3513 Experts worldwide ranked by ideXlab platform
Shivam Priya - One of the best experts on this subject based on the ideXlab platform.
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Diethyl Maleate inhibits mca tpa transformed cell growth via modulation of gsh mapk and cancer pathways
Chemico-Biological Interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
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Diethyl Maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways.
Chemico-biological interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
Sushil Kumar - One of the best experts on this subject based on the ideXlab platform.
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Diethyl Maleate inhibits mca tpa transformed cell growth via modulation of gsh mapk and cancer pathways
Chemico-Biological Interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
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Diethyl Maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways.
Chemico-biological interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
Akanksha Nigam - One of the best experts on this subject based on the ideXlab platform.
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Diethyl Maleate inhibits mca tpa transformed cell growth via modulation of gsh mapk and cancer pathways
Chemico-Biological Interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
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Diethyl Maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways.
Chemico-biological interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
Tugba Gündogdu - One of the best experts on this subject based on the ideXlab platform.
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phase equilibria of water carboxylic acid Diethyl Maleate ternary liquid systems at 298 15 k
Fluid Phase Equilibria, 2011Co-Authors: Süheyla Çehreli, Tugba GündogduAbstract:Abstract Liquid–liquid equilibrium (LLE) data for the ternary systems of (water–formic acid–Diethyl Maleate), (water–acetic acid–Diethyl Maleate), (water–propionic acid–Diethyl Maleate), (water–butyric acid–Diethyl Maleate), and (water–valeric acid–Diethyl Maleate) were investigated at 298.15 K and atmospheric pressure. Complete phase diagrams were obtained by determining solubility and the tie-line data. The tie-line data were compared with the results predicted by the UNIFAC and the modified UNIFAC (Dortmund) methods and correlated by means of UNIQUAC model. The reliability of the experimental tie-line data was confirmed by using the Othmer–Tobias correlation. Distribution coefficients and selectivity were evaluated for the immiscibility region.
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Phase equilibria of (water–carboxylic acid–Diethyl Maleate) ternary liquid systems at 298.15 K
Fluid Phase Equilibria, 2011Co-Authors: Süheyla Çehreli, Tugba GündogduAbstract:Abstract Liquid–liquid equilibrium (LLE) data for the ternary systems of (water–formic acid–Diethyl Maleate), (water–acetic acid–Diethyl Maleate), (water–propionic acid–Diethyl Maleate), (water–butyric acid–Diethyl Maleate), and (water–valeric acid–Diethyl Maleate) were investigated at 298.15 K and atmospheric pressure. Complete phase diagrams were obtained by determining solubility and the tie-line data. The tie-line data were compared with the results predicted by the UNIFAC and the modified UNIFAC (Dortmund) methods and correlated by means of UNIQUAC model. The reliability of the experimental tie-line data was confirmed by using the Othmer–Tobias correlation. Distribution coefficients and selectivity were evaluated for the immiscibility region.
Preeti Bajpai - One of the best experts on this subject based on the ideXlab platform.
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Diethyl Maleate inhibits mca tpa transformed cell growth via modulation of gsh mapk and cancer pathways
Chemico-Biological Interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.
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Diethyl Maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways.
Chemico-biological interactions, 2014Co-Authors: Shivam Priya, Akanksha Nigam, Preeti Bajpai, Sushil KumarAbstract:Abstract Murine or human cancer cells have high glutathione levels. Depletion of the elevated GSH inhibits proliferation of cancer cells. Molecular basis for this observation is little understood. In an attempt to find out the underlying mechanism, we reproduced these effects in transformed C3H10T1/2 and BALB/c 3T3 cells using Diethyl Maleate and studied cytogenomic changes in the whole mouse genome using spotted 8 × 60K arrays. Transformed cells revealed an increase in GSH levels. GSH depletion by DEM inhibited the growth of transformed cells. The non-cytotoxic dose of DEM (0.25 mM) resulted in GSH depletion, ROS generation, cell cycle arrest, apoptosis, decrease in anchorage independent growth, gene expression changes and activation of all three members of the MAPK family. Increase in intracellular GSH levels by GSHe countered the effect of DEM. These results support the physiological importance of GSH in regulation of gene expression for transformed cell growth restraint. This study is of interest in not only understanding the molecular biology of the transformed cells, but also in identifying new targets for development of gene therapy together with the chemotherapy.