The Experts below are selected from a list of 1002 Experts worldwide ranked by ideXlab platform
Cheng-bin Cui - One of the best experts on this subject based on the ideXlab platform.
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Penicimutamides A–C: rare carbamate-containing alkaloids from a mutant of the marine-derived Penicillium purpurogenum G59
RSC Advances, 2016Co-Authors: Cheng-bin Cui, Fei Cao, Hua-jie ZhuAbstract:Three rare carbamate-containing alkaloids, penicimutamides A–C (1–3), were isolated from a fungal mutant from the Diethyl Sulfate (DES) mutagenesis of marine-derived Penicillium purpurogenum G59. Their structures, including their absolute configurations, were determined by spectroscopic methods, especially the X-ray crystallography and CD analyses. HPLC-UV and HPLC-MS analyses evidenced that 1–3 were only produced in the mutant strain via biosynthetic pathways that were silent in the parental strain and activated by DES mutagenesis.
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A novel oxaphenalenone, penicimutalidine: activated production of oxaphenalenones by the Diethyl sulphate mutagenesis of marine-derived fungus Penicillium purpurogenum G59
RSC Advances, 2016Co-Authors: Ming-wen Xia, Cheng-bin Cui, Ji-xing PengAbstract:A novel oxaphenalenone, penicimutalidine (1), was isolated from a fungal mutant generated through the Diethyl Sulfate (DES) mutagenesis of marine-derived Penicillium purpurogenum G59 along with three known oxaphenalenones (2–4). The structure of 1, including the absolute configuration, was determined by spectroscopic methods, including TDDFT electronic CD calculations. HPLC-UV/HPLC-MS analyses verified that 1–4 were only produced in the mutant through the activation of silent biosynthetic pathways in the parent strain by DES mutagenesis.
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Purpurogemutantin and Purpurogemutantidin, New Drimenyl Cyclohexenone Derivatives Produced by a Mutant Obtained by Diethyl Sulfate Mutagenesis of a Marine-Derived Penicillium purpurogenum G59
Marine Drugs, 2012Co-Authors: Shiming Fang, Zhijun Zhang, Cheng-bin Cui, Xiaojun HuangAbstract:Two new drimenyl cyclohexenone derivatives, named purpurogemutantin (<strong>1</strong>) and purpurogemutantidin (<strong>2</strong>), and the known macrophorin A (<strong>3</strong>) were isolated from a bioactive mutant BD-1-6 obtained by random Diethyl Sulfate (DES) mutagenesis of a marine-derived <em>Penicillium purpurogenum</em> G59. Structures and absolute configurations of <strong>1</strong> and <strong>2</strong> were determined by extensive spectroscopic methods, especially 2D NMR and electronic circular dichroism (ECD) analysis. Possible biosynthetic pathways for <strong>1</strong>–<strong>3</strong> were also proposed and discussed. Compounds <strong>1</strong> and <strong>2</strong> significantly inhibited human cancer K562, HL-60, HeLa, BGC-823 and MCF-7 cells, and compound <strong>3</strong> also inhibited the K562 and HL-60 cells. Both bioassay and chemical analysis (HPLC, LC-ESIMS) demonstrated that the parent strain G59 did not produce <strong>1</strong>–<strong>3</strong>, and that DES-induced mutation(s) in the mutant BD-1-6 activated some silent biosynthetic pathways in the parent strain G59, including one set for <strong>1</strong>–<strong>3</strong> production
Wei Jiang - One of the best experts on this subject based on the ideXlab platform.
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Selection of Zygosaccharomyces rouxii strains resistant to cadmium with improved removal abilities through ultraviolet-Diethyl Sulfate cooperative mutagenesis.
Environmental science and pollution research international, 2017Co-Authors: Yu Liu, Dongfeng Wang, Wei JiangAbstract:Cd2+ resistance and bioaccumulation capacity were selected from parental Zygosaccharomyces rouxii (CRZ-0) while maintaining NaCl tolerance using protoplast mutagenesis technology. Ultraviolet-Diethyl Sulfate (UV-DES) cooperative mutagenesis, followed by preliminary screening and rescreening, was used to select the mutant strain CRZ-9. CRZ-9 grew better than CRZ-0 in YPD medium with 20 or 50 mg L−1 of Cd2+. Scanning electron microscopy observations and flow cytometry tests indicated that CRZ-9 was more effective at eliminating reactive oxygen species (ROS) generated by Cd2+, which led to less cellular structural damage and lower lethality. Furthermore, compared with CRZ-0, CRZ-9 exhibited increased potential for application with higher Cd2+ removal ratio, wider working pH range, and lower biomass dosage in Cd2+ bioaccumulation. The mutant strain CRZ-9 possessed improved Cd2+ resistance and bioaccumulation capacity and therefore is a promising strain to remove Cd2+ from wastewater.
Peng Zhao - One of the best experts on this subject based on the ideXlab platform.
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Diethyl Sulfate-induced cell cycle arrest and apoptosis in human bronchial epithelial 16HBE cells
Chemico-biological interactions, 2013Co-Authors: Peng Zhao, Biyun Yao, Yanchao Song, Hongtao Zhang, Yongrui JiaAbstract:Abstract In this study, we investigated the effects of Diethyl Sulfate (DES) on cell proliferation, cell cycle progression and apoptosis in human bronchial epithelial 16HBE cells. Cells were treated with various doses of DES (0, 0.5, 1.0, 2.0, 4.0 or 8.0 mM) for 12, 24 or 36 h. Cell proliferation and apoptosis were determined by MTT assay and flow cytometer, respectively. The results showed that DES inhibited cell proliferation in a dose- and time-dependent manner, and induced significant apoptosis in 16HBE cells. Apoptosis related proteins measurement results revealed that DES-induced apoptosis was concurrent with the increasing of Bax and cleavage fragment caspase-3 and the decreasing of Bcl-2 and full length procaspase-3. When cells were incubated with 2.0 mM of DES for several time intervals, S and G2/M phase accumulation was observed. Further analysis indicated that both DES-induced G1/S transition acceleration and S arrest resulted in S phase accumulation, and that DES-induced G2/M arrest resulted in G2/M phase accumulation. Western blotting results demonstrated that after DES treatment p-chk1 (Ser345) and p-chk2 (Thr68) levels decreased in G1 cells, and increased in S and G2/M cells. In addition, the increasing of chk1 and chk2 were also induced by DES treatment. With the increase in the dose of DES, p53 levels first increased (0.5–4.0 mM) and then decreased (8.0 mM). Down-regulation of p53 by RNA interference increased 4.0 mM of DES-induced apoptosis but did not affect 2.0 mM DES-induced cell cycle arrest. In conclusion, DES inhibits 16HBE cells proliferation in a dose- and time-dependent behavior. Within the sublethal dose, DES induces S and G2/M arrest through activating DNA damage checkpoints. Within the lethal dose, DES induces apoptosis through evoking apoptosis programs. p53 might play an important role in the transition between evoking cell cycle arrest/pro-survival and apoptosis programs upon DES exposure.
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Diethyl Sulfate induced Cdk2-dependent centrosome amplification in CHL cells.
Toxicology, 2010Co-Authors: Peng Zhao, Biyun Yao, Zhun Yuan, Zongcan ZhouAbstract:The function of centrosome that serves as the main microtubule organizing center is essential to ensure the genomic integrity during the cell division cycle. Centrosome abnormalities are frequently observed in many tumors and cells exposed to genotoxic agents. Here, we investigated the centrosome abnormalities induced by Diethyl Sulfate (DES) in Chinese hamster lung (CHL) fibroblasts and the underlying molecular mechanisms. Our results showed that DES exposure at 0.3, 1 and 3mM for 48 h caused centrosome amplification in a dose dependent manner. This effect was associated with transient S and G2/M phase delay and up-regulating of Cdk2, Cyclin A expressions. Furthermore, inhibition of Cdk2 activities reversed the centrosome amplification induced by DES. These results reveal that centrosome is one of the key subcellular targets of DES. Centrosome abnormalities might be important mechanisms behind the aneuploidy induction and carcinogenicity of DES.
Xiaojun Huang - One of the best experts on this subject based on the ideXlab platform.
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purpurogemutantin and purpurogemutantidin new drimenyl cyclohexenone derivatives produced by a mutant obtained by Diethyl Sulfate mutagenesis of a marine derived penicillium purpurogenum g59
Marine Drugs, 2012Co-Authors: Shiming Fang, Changwei Li, Changjing Wu, Zhijun Zhang, Li Li, Xiaojun Huang, Wencai YeAbstract:Two new drimenyl cyclohexenone derivatives, named purpurogemutantin (1) and purpurogemutantidin (2), and the known macrophorin A (3) were isolated from a bioactive mutant BD-1-6 obtained by random Diethyl Sulfate (DES) mutagenesis of a marine-derived Penicillium purpurogenum G59. Structures and absolute configurations of 1 and 2 were determined by extensive spectroscopic methods, especially 2D NMR and electronic circular dichroism (ECD) analysis. Possible biosynthetic pathways for 1–3 were also proposed and discussed. Compounds 1 and 2 significantly inhibited human cancer K562, HL-60, HeLa, BGC-823 and MCF-7 cells, and compound 3 also inhibited the K562 and HL-60 cells. Both bioassay and chemical analysis (HPLC, LC-ESIMS) demonstrated that the parent strain G59 did not produce 1–3, and that DES-induced mutation(s) in the mutant BD-1-6 activated some silent biosynthetic pathways in the parent strain G59, including one set for 1–3 production.
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Purpurogemutantin and Purpurogemutantidin, New Drimenyl Cyclohexenone Derivatives Produced by a Mutant Obtained by Diethyl Sulfate Mutagenesis of a Marine-Derived Penicillium purpurogenum G59
Marine Drugs, 2012Co-Authors: Shiming Fang, Zhijun Zhang, Cheng-bin Cui, Xiaojun HuangAbstract:Two new drimenyl cyclohexenone derivatives, named purpurogemutantin (<strong>1</strong>) and purpurogemutantidin (<strong>2</strong>), and the known macrophorin A (<strong>3</strong>) were isolated from a bioactive mutant BD-1-6 obtained by random Diethyl Sulfate (DES) mutagenesis of a marine-derived <em>Penicillium purpurogenum</em> G59. Structures and absolute configurations of <strong>1</strong> and <strong>2</strong> were determined by extensive spectroscopic methods, especially 2D NMR and electronic circular dichroism (ECD) analysis. Possible biosynthetic pathways for <strong>1</strong>–<strong>3</strong> were also proposed and discussed. Compounds <strong>1</strong> and <strong>2</strong> significantly inhibited human cancer K562, HL-60, HeLa, BGC-823 and MCF-7 cells, and compound <strong>3</strong> also inhibited the K562 and HL-60 cells. Both bioassay and chemical analysis (HPLC, LC-ESIMS) demonstrated that the parent strain G59 did not produce <strong>1</strong>–<strong>3</strong>, and that DES-induced mutation(s) in the mutant BD-1-6 activated some silent biosynthetic pathways in the parent strain G59, including one set for <strong>1</strong>–<strong>3</strong> production
Shiming Fang - One of the best experts on this subject based on the ideXlab platform.
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purpurogemutantin and purpurogemutantidin new drimenyl cyclohexenone derivatives produced by a mutant obtained by Diethyl Sulfate mutagenesis of a marine derived penicillium purpurogenum g59
Marine Drugs, 2012Co-Authors: Shiming Fang, Changwei Li, Changjing Wu, Zhijun Zhang, Li Li, Xiaojun Huang, Wencai YeAbstract:Two new drimenyl cyclohexenone derivatives, named purpurogemutantin (1) and purpurogemutantidin (2), and the known macrophorin A (3) were isolated from a bioactive mutant BD-1-6 obtained by random Diethyl Sulfate (DES) mutagenesis of a marine-derived Penicillium purpurogenum G59. Structures and absolute configurations of 1 and 2 were determined by extensive spectroscopic methods, especially 2D NMR and electronic circular dichroism (ECD) analysis. Possible biosynthetic pathways for 1–3 were also proposed and discussed. Compounds 1 and 2 significantly inhibited human cancer K562, HL-60, HeLa, BGC-823 and MCF-7 cells, and compound 3 also inhibited the K562 and HL-60 cells. Both bioassay and chemical analysis (HPLC, LC-ESIMS) demonstrated that the parent strain G59 did not produce 1–3, and that DES-induced mutation(s) in the mutant BD-1-6 activated some silent biosynthetic pathways in the parent strain G59, including one set for 1–3 production.
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Purpurogemutantin and Purpurogemutantidin, New Drimenyl Cyclohexenone Derivatives Produced by a Mutant Obtained by Diethyl Sulfate Mutagenesis of a Marine-Derived Penicillium purpurogenum G59
Marine Drugs, 2012Co-Authors: Shiming Fang, Zhijun Zhang, Cheng-bin Cui, Xiaojun HuangAbstract:Two new drimenyl cyclohexenone derivatives, named purpurogemutantin (<strong>1</strong>) and purpurogemutantidin (<strong>2</strong>), and the known macrophorin A (<strong>3</strong>) were isolated from a bioactive mutant BD-1-6 obtained by random Diethyl Sulfate (DES) mutagenesis of a marine-derived <em>Penicillium purpurogenum</em> G59. Structures and absolute configurations of <strong>1</strong> and <strong>2</strong> were determined by extensive spectroscopic methods, especially 2D NMR and electronic circular dichroism (ECD) analysis. Possible biosynthetic pathways for <strong>1</strong>–<strong>3</strong> were also proposed and discussed. Compounds <strong>1</strong> and <strong>2</strong> significantly inhibited human cancer K562, HL-60, HeLa, BGC-823 and MCF-7 cells, and compound <strong>3</strong> also inhibited the K562 and HL-60 cells. Both bioassay and chemical analysis (HPLC, LC-ESIMS) demonstrated that the parent strain G59 did not produce <strong>1</strong>–<strong>3</strong>, and that DES-induced mutation(s) in the mutant BD-1-6 activated some silent biosynthetic pathways in the parent strain G59, including one set for <strong>1</strong>–<strong>3</strong> production