The Experts below are selected from a list of 1569 Experts worldwide ranked by ideXlab platform
P Perocco - One of the best experts on this subject based on the ideXlab platform.
-
in vitro cytotoxic and cell transforming activities exerted by the pesticides cyanazine dithianon Diflubenzuron procymidone and vinclozolin on balb c 3t3 cells
Environmental and Molecular Mutagenesis, 1993Co-Authors: P Perocco, Annamaria Colacci, Simonetta GrilliAbstract:Cytotoxic and cell transforming activities of the pesticides cyanazine, Diflubenzuron, dithianon, procymidone, and vinclozolin were investigated in vitro by utilizing the BALB/c 3T3 cell transformation test performed in the presence or in the absence of S-9 mix as an exogenous bioactivation system for the chemicals. All the assayed pesticides were cytotoxic in the absence of S-9 mix, whereas only dithianon exerted cytotoxic effects in the presence of metabolic activation. All the chemicals tested did induce BALB/c 3T3 cell transformation, to a various extent, in the absence of S-9 mix. Cell transforming ability of cyanazine and Diflubenzuron was not detectable in the presence of S-9. © 1993 Wiley-Liss, Inc.
-
in vitro cytotoxic and cell transforming activities exerted by the pesticides cyanazine dithianon Diflubenzuron procymidone and vinclozolin on balb c 3t3 cells
Environmental and Molecular Mutagenesis, 1993Co-Authors: P Perocco, Annamaria Colacci, Sandro GrilliAbstract:Cytotoxic and cell transforming activities of the pesticides cyanazine, Diflubenzuron, dithianon, procymidone, and vinclozolin were investigated in vitro by utilizing the BALB/c 3T3 cell transformation test performed in the presence or in the absence of S-9 mix as an exogenous bioactivation system for the chemicals. All the assayed pesticides were cytotoxic in the absence of S-9 mix, whereas only dithianon exerted cytotoxic effects in the presence of metabolic activation. All the chemicals tested did induce BALB/c 3T3 cell transformation, to a various extent, in the absence of S-9 mix. Cell transforming ability of cyanazine and Diflubenzuron was not detectable in the presence of S-9.
Koon Weng Lau - One of the best experts on this subject based on the ideXlab platform.
-
evaluation of insect growth regulators against field collected aedes aegypti and aedes albopictus diptera culicidae from malaysia
Journal of Medical Entomology, 2015Co-Authors: Koon Weng Lau, Chee Dhang Chen, H L Lee, Y Normarashid, Mohd SofianazirunAbstract:Susceptibility status of Aedes aegypti (L.) and Aedes albopictus Skuse larvae obtained from 12 states in Malaysia were evaluated against five insect growth regulators (IGRs), namely, pyriproxyfen, methoprene, Diflubenzuron, cyromazine, and novaluron under laboratory conditions. Field populations of Ae. aegypti exhibited moderate resistance toward methoprene and low resistance toward pyriproxyfen, with resistance ratios of 12.7 and 1.4, respectively, but susceptibility to Diflubenzuron, cyromazine, and novaluron. On the other hand, field populations of Ae. albopictus exhibited low resistance against Diflubenzuron and novaluron, with resistance ratio of 2.1 and 1.0, respectively, but susceptibility to other tested IGRs. Our study concluded that the tested IGRs provide promising results and can be used to control field population of Ae. aegypti and Ae. albopictus, especially cyromazine. The use of IGR should be considered as an alternative when larvae develop resistance to conventional insecticides.
Huili Wang - One of the best experts on this subject based on the ideXlab platform.
-
the body burden and thyroid disruption in lizards eremias argus living in benzoylurea pesticides contaminated soil
Journal of Hazardous Materials, 2018Co-Authors: Jing Chang, Huili Wang, Jitong Li, Wei Li, Jianzhong Li, Yinghuan Wang, Peng XuAbstract:Abstract Dermal exposure is regarded as a potentially significant but understudied route for pesticides uptake in terrestrial reptiles. In this study, a native Chinese lizard was exposed to control, Diflubenzuron or flufenoxuron contaminated soil (1.5 mg kg−1) for 35 days. Tissue distribution, liver lesions, thyroid hormone levels and transcription of most target genes were examined. The half-lives of Diflubenzuron and flufenoxuron in the soil were 118.9 and 231.8 days, respectively. The accumulation of flufenoxuron in the liver, brain, kidney, heart, plasma and skin (1.4–35.4 mg kg−1) were higher than that of Diflubenzuron (0–1.7 mg kg−1) at all time points. The skin permeability factor of flufenoxuron was more than 20-fold greater than that of Diflubenzuron at the end of exposure. However, the liver was more vulnerable in the Diflubenzuron exposure group. The alterations of triiodothyronine (T3) and thyroxine (T4) level after Diflubenzuron or flufenoxuron exposure were accompanied with the changes in the transcription of target genes involved not only in hypothalamus-pituitary-thyroid (HPT) axis (sult, dio2, trα and udp) but also in metabolism system (cyp1a and ahr). These results indicated that flufenoxuron produced greater body burdens to lizards through dermal exposure, whereas both Diflubenzuron and flufenoxuron have the potential to disturb metabolism and thyroid endocrine system.
-
Metabolism of Diflubenzuron in Lizard (Eremias argus) and Comparative Toxicity of Diflubenzuron and Its Metabolite
2018Co-Authors: Huili Wang, Yun Xie, Meng Jiao, Yanfeng Zhang, Jing ChangAbstract:The metabolic process of Diflubenzuron in rat or fish has been well studied, but little is known about its elimination pathway in lizard. The current study predicted the metabolic route of Diflubenzuron in lizard feces and compared the toxicity of Diflubenzuron and 4-chloroaniline on lizard thyroid system. The amido bond cleavage was the major route for Diflubenzuron elimination in lizard feces. 4-Chloroaniline as the most toxic Diflubenzuron metabolite was also abundant in feces. According to liver slices, 4-chloroaniline exposure induced significant changes of nuclear shape, while Diflubenzuron exposure caused significant hepatocytes clustering. On the basis of thyroid hormone and thyroid-related gene levels, triiodothyronine (T3) level in lizard liver was regulated by thyroid hormone receptors, while thyroxine (T4) concentration was modulated by dio2 and udp genes after Diflubenzuron or 4-chloroaniline exposure. These results showed that both Diflubenzuron and 4-chloroaniline could disrupt lizard thyroid system, which could provide evidence for lizard population decline
-
the tissue distribution metabolism and hepatotoxicity of benzoylurea pesticides in male eremias argus after a single oral administration
Chemosphere, 2017Co-Authors: Jing Chang, Yinghuan Wang, Baoyuan Guo, Huili WangAbstract:Benzoylurea pesticides (BPUs) are widely used to control the locust, but the toxicokinetics and hepatotoxicity of BPUs in lizards have not been investigated. In this study, the tissue distribution, metabolism and liver toxicity of Diflubenzuron and flufenoxuron were assessed in the Eremias argus following a single oral exposure. Diflubenzuron preferred to accumulate in the fat and brain (>1.0 mg kg-1) and was rapidly eliminate in other tissues. In the liver, 4-chloroaniline was one of Diflubenzuron metabolites, although with a concentration less than 0.05% of the accumulated Diflubenzuron. No significant difference was observed in the liver histopathology between the control and Diflubenzuron exposure group. The expressions of Cyp1a and Ahr gene which control the cell apoptosis were also equal to the control level. After flufenoxuron exposure, biomodal phenomenon was observed in the liver, skin, brain, gonad, kidney, heart and blood circulation was an important route for the flufenoxuron penetration. The concentrations of flufenoxuron in all tissues were greater than 1.0 mg kg-1 at 168 h. The excretion of flufenoxuron in the faeces was 1.5 fold higher than Diflubenzuron. The hepatocytes in the flufenoxuron treated group showed vacuolation of cytoplasm and decreased nucleus. In addition, the Cyp1a and Ahr genes were significantly up-regulated in the flufenoxuron exposure group. These results suggested that the higher hepatotoxicity of flufenoxuron may be attributed to the higher residual level in the lizard tissues and the Cyp1a and Ahr genes can serve as biomarkers to assess the liver toxicity.
Jing Chang - One of the best experts on this subject based on the ideXlab platform.
-
the body burden and thyroid disruption in lizards eremias argus living in benzoylurea pesticides contaminated soil
Journal of Hazardous Materials, 2018Co-Authors: Jing Chang, Huili Wang, Jitong Li, Wei Li, Jianzhong Li, Yinghuan Wang, Peng XuAbstract:Abstract Dermal exposure is regarded as a potentially significant but understudied route for pesticides uptake in terrestrial reptiles. In this study, a native Chinese lizard was exposed to control, Diflubenzuron or flufenoxuron contaminated soil (1.5 mg kg−1) for 35 days. Tissue distribution, liver lesions, thyroid hormone levels and transcription of most target genes were examined. The half-lives of Diflubenzuron and flufenoxuron in the soil were 118.9 and 231.8 days, respectively. The accumulation of flufenoxuron in the liver, brain, kidney, heart, plasma and skin (1.4–35.4 mg kg−1) were higher than that of Diflubenzuron (0–1.7 mg kg−1) at all time points. The skin permeability factor of flufenoxuron was more than 20-fold greater than that of Diflubenzuron at the end of exposure. However, the liver was more vulnerable in the Diflubenzuron exposure group. The alterations of triiodothyronine (T3) and thyroxine (T4) level after Diflubenzuron or flufenoxuron exposure were accompanied with the changes in the transcription of target genes involved not only in hypothalamus-pituitary-thyroid (HPT) axis (sult, dio2, trα and udp) but also in metabolism system (cyp1a and ahr). These results indicated that flufenoxuron produced greater body burdens to lizards through dermal exposure, whereas both Diflubenzuron and flufenoxuron have the potential to disturb metabolism and thyroid endocrine system.
-
Metabolism of Diflubenzuron in Lizard (Eremias argus) and Comparative Toxicity of Diflubenzuron and Its Metabolite
2018Co-Authors: Huili Wang, Yun Xie, Meng Jiao, Yanfeng Zhang, Jing ChangAbstract:The metabolic process of Diflubenzuron in rat or fish has been well studied, but little is known about its elimination pathway in lizard. The current study predicted the metabolic route of Diflubenzuron in lizard feces and compared the toxicity of Diflubenzuron and 4-chloroaniline on lizard thyroid system. The amido bond cleavage was the major route for Diflubenzuron elimination in lizard feces. 4-Chloroaniline as the most toxic Diflubenzuron metabolite was also abundant in feces. According to liver slices, 4-chloroaniline exposure induced significant changes of nuclear shape, while Diflubenzuron exposure caused significant hepatocytes clustering. On the basis of thyroid hormone and thyroid-related gene levels, triiodothyronine (T3) level in lizard liver was regulated by thyroid hormone receptors, while thyroxine (T4) concentration was modulated by dio2 and udp genes after Diflubenzuron or 4-chloroaniline exposure. These results showed that both Diflubenzuron and 4-chloroaniline could disrupt lizard thyroid system, which could provide evidence for lizard population decline
-
the tissue distribution metabolism and hepatotoxicity of benzoylurea pesticides in male eremias argus after a single oral administration
Chemosphere, 2017Co-Authors: Jing Chang, Yinghuan Wang, Baoyuan Guo, Huili WangAbstract:Benzoylurea pesticides (BPUs) are widely used to control the locust, but the toxicokinetics and hepatotoxicity of BPUs in lizards have not been investigated. In this study, the tissue distribution, metabolism and liver toxicity of Diflubenzuron and flufenoxuron were assessed in the Eremias argus following a single oral exposure. Diflubenzuron preferred to accumulate in the fat and brain (>1.0 mg kg-1) and was rapidly eliminate in other tissues. In the liver, 4-chloroaniline was one of Diflubenzuron metabolites, although with a concentration less than 0.05% of the accumulated Diflubenzuron. No significant difference was observed in the liver histopathology between the control and Diflubenzuron exposure group. The expressions of Cyp1a and Ahr gene which control the cell apoptosis were also equal to the control level. After flufenoxuron exposure, biomodal phenomenon was observed in the liver, skin, brain, gonad, kidney, heart and blood circulation was an important route for the flufenoxuron penetration. The concentrations of flufenoxuron in all tissues were greater than 1.0 mg kg-1 at 168 h. The excretion of flufenoxuron in the faeces was 1.5 fold higher than Diflubenzuron. The hepatocytes in the flufenoxuron treated group showed vacuolation of cytoplasm and decreased nucleus. In addition, the Cyp1a and Ahr genes were significantly up-regulated in the flufenoxuron exposure group. These results suggested that the higher hepatotoxicity of flufenoxuron may be attributed to the higher residual level in the lizard tissues and the Cyp1a and Ahr genes can serve as biomarkers to assess the liver toxicity.
Sandro Grilli - One of the best experts on this subject based on the ideXlab platform.
-
in vitro cytotoxic and cell transforming activities exerted by the pesticides cyanazine dithianon Diflubenzuron procymidone and vinclozolin on balb c 3t3 cells
Environmental and Molecular Mutagenesis, 1993Co-Authors: P Perocco, Annamaria Colacci, Sandro GrilliAbstract:Cytotoxic and cell transforming activities of the pesticides cyanazine, Diflubenzuron, dithianon, procymidone, and vinclozolin were investigated in vitro by utilizing the BALB/c 3T3 cell transformation test performed in the presence or in the absence of S-9 mix as an exogenous bioactivation system for the chemicals. All the assayed pesticides were cytotoxic in the absence of S-9 mix, whereas only dithianon exerted cytotoxic effects in the presence of metabolic activation. All the chemicals tested did induce BALB/c 3T3 cell transformation, to a various extent, in the absence of S-9 mix. Cell transforming ability of cyanazine and Diflubenzuron was not detectable in the presence of S-9.