The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
Pavol Balgavý - One of the best experts on this subject based on the ideXlab platform.
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Determination of bilayer thickness and lipid surface area in unilamellar Dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods
European Biophysics Journal, 2004Co-Authors: Norbert Kučerka, Mikhail A. Kiselev, Pavol BalgavýAbstract:Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-Dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 Å pores. The data obtained at 30±0.1 °C were evaluated using a five-strip function model of the bilayer coherent neutron scattering length density, three different approximate form factors describing scattering from vesicles, and different methods of evaluation of the experimental data. It is shown that the results obtained from the SANS data in the range of scattering vector values 0.0316 Å^−1< q
Mikhail A. Kiselev - One of the best experts on this subject based on the ideXlab platform.
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Investigation of the structure of unilamellar Dimyristoylphosphatidylcholine vesicles in aqueous sucrose solutions by small-angle neutron and X-ray scattering
Crystallography Reports, 2015Co-Authors: Mikhail A. Kiselev, Elena Zemlyanaya, E. I. Zhabitskaya, V. L. AksenovAbstract:The structure of a polydispersed population of unilamellar Dimyristoylphosphatidylcholine (DMPC) vesicles in sucrose solutions has been investigated by small-angle neutron scattering (SANS) and small-angle X-ray scattering (SAXS). Calculations within the model of separated form factors (SFF) show that the structure of the vesicle system depends strongly on the sucrose concentration.
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Determination of bilayer thickness and lipid surface area in unilamellar Dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods
European Biophysics Journal, 2004Co-Authors: Norbert Kučerka, Mikhail A. Kiselev, Pavol BalgavýAbstract:Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-Dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 Å pores. The data obtained at 30±0.1 °C were evaluated using a five-strip function model of the bilayer coherent neutron scattering length density, three different approximate form factors describing scattering from vesicles, and different methods of evaluation of the experimental data. It is shown that the results obtained from the SANS data in the range of scattering vector values 0.0316 Å^−1< q
Göran Lindblom - One of the best experts on this subject based on the ideXlab platform.
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effect of sterol structure on the bending rigidity of lipid membranes a 2h nmr transverse relaxation study
Biochimica et Biophysica Acta, 2009Co-Authors: Greger Orädd, Vahid Shahedi, Göran LindblomAbstract:The effect of incorporation of 3–43 mol% sterol on the lipid order and bilayer rigidity has been investigated for model membranes of Dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine ...
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Farnesylated peptides in model membranes: a biophysical investigation
European Biophysics Journal, 2004Co-Authors: Amy C. Rowat, Göran Lindblom, Jesper Brask, Tobias Sparrman, Knud J. Jensen, John H. IpsenAbstract:Protein prenylation plays an important role in signal transduction, protein–protein interactions, and the localization and association of proteins with membranes. Using three different techniques, this study physically characterizes the interactions between model Dimyristoylphosphatidylcholine membranes and a series of farnesylated peptides. Magic angle spinning nuclear Overhauser enhancement spectroscopy and differential scanning calorimetry reveal that both charged [Ac-Asn-Lys-Asn-Cys-(farnesyl)-OMe and Ac-Asn-Lys-Asn-Cys-(farnesyl)-NH_2] and uncharged [Ac-Cys-(farnesyl)-OMe and farnesol] species partition into Dimyristoylphosphatidylcholine bilayers. Calorimetry and vesicle fluctuation analysis of giant unilamellar vesicles show that the charged peptides modestly decrease the main gel–fluid phase transition and markedly increase the bending rigidity of large unilamellar vesicles. Uncharged species, on the other hand, dramatically decrease the main phase transition and modestly decrease the bending rigidity. No difference with carboxyl methylation is detected.
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lateral diffusion of cholesterol and Dimyristoylphosphatidylcholine in a lipid bilayer measured by pulsed field gradient nmr spectroscopy
Biophysical Journal, 2002Co-Authors: Greger Orädd, Göran Lindblom, P W WestermanAbstract:The pulsed field gradient NMR method for measuring self-diffusion has been used for a direct determination of the lateral diffusion coefficient of cholesterol, fluorine labeled at the 6-position, for an oriented lamellar liquid-crystalline phase of Dimyristoylphosphatidylcholine (DMPC)/cholesterol/water. It is found that the diffusion coefficients of DMPC and cholesterol are equal over a large temperature interval. The apparent energy of activation for the diffusion process (58 kJ/mol) is about the same as for a lamellar phase of DMPC/water, whereas the phospholipid lateral diffusion coefficient is approximately four times smaller in the presence of cholesterol.
Norbert Kučerka - One of the best experts on this subject based on the ideXlab platform.
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Determination of bilayer thickness and lipid surface area in unilamellar Dimyristoylphosphatidylcholine vesicles from small-angle neutron scattering curves: a comparison of evaluation methods
European Biophysics Journal, 2004Co-Authors: Norbert Kučerka, Mikhail A. Kiselev, Pavol BalgavýAbstract:Small-angle neutron scattering (SANS) experiments were performed on unilamellar 1,2-Dimyristoylphosphatidylcholine (DMPC) vesicles prepared in heavy water by extrusion through polycarbonate filters with 500 Å pores. The data obtained at 30±0.1 °C were evaluated using a five-strip function model of the bilayer coherent neutron scattering length density, three different approximate form factors describing scattering from vesicles, and different methods of evaluation of the experimental data. It is shown that the results obtained from the SANS data in the range of scattering vector values 0.0316 Å^−1< q
John Katsaras - One of the best experts on this subject based on the ideXlab platform.
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STRUCTURE OF THE RIPPLE PHASE IN CHIRAL AND RACEMIC Dimyristoylphosphatidylcholine MULTIBILAYERS
Physical Review E, 1999Co-Authors: Kheya Sengupta, V. A. Raghunathan, John KatsarasAbstract:We present electron density maps of the ripple phase of chiral and racemic Dimyristoylphosphatidylcholine. The structures of the two systems are found to be identical within experimental errors, thus unambiguously showing that the chirality of the lipid molecules does not influence the structure of this phase.
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Adsorbed to a rigid substrate, Dimyristoylphosphatidylcholine multibilayers attain full hydration in all mesophases.
Biophysical journal, 1998Co-Authors: John KatsarasAbstract:Whether hydrated from vapor or immersed in liquid water, aligned multibilayers of Dimyristoylphosphatidylcholine adsorbed to a single mica "substrate" are shown by neutron diffraction to hydrate in all mesophases (e.g., Lbeta', Pbeta', and Lalpha) to the same extent as their liposomal counterparts suspended in liquid water. These data clearly demonstrate that the commonly accepted vapor pressure paradox does not exist.