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A. F. Vanin - One of the best experts on this subject based on the ideXlab platform.

  • Hypotensive Effect and Accumulation of Dinitrosyl Iron Complexes in Blood and Tissues after Intravenous and Subcutaneous Injection
    Bulletin of Experimental Biology and Medicine, 2016
    Co-Authors: A. A. Timoshin, V. L. Lakomkin, A. A. Abramov, E. K. Ruuge, A. F. Vanin
    Abstract:

    Subcutaneous injection of Oxacom with glutathione-bound dinitrosyl iron Complex as the active principle produced a slower drop of mean BP and longer accumulation of protein-bound dinitrosyl iron Complexes in whole blood and tissues than intravenous injection of this drug, while durations of hypotensive effect in both cases were practically identical. In contrast to intravenous injection of the drug, its subcutaneous administration was not characterized by a high concentration of protein-bound dinitrosyl iron Complexes in the blood at the onset of experiment; in addition, accumulation of these NO forms in the lungs was more pronounced after subcutaneous injection than after intravenous one.

  • Detection of autowave distribution of the concentration of a dinitrosyl iron Complex with glutathione formed in an aqueous solution of S-nitrosoglutathione after addition of a mixture of glutathione and ferrous iron
    Biophysics, 2010
    Co-Authors: A. F. Vanin, V. D. Mikoyan, L. N. Kubrina
    Abstract:

    The formation of dark green concentric autowaves of the distribution of the concentration of dinitrosyl iron Complex (DNIC) with glutathione in a thin (0.3 mm thick) layer of 0.5 M solution of S-nitrosoglutathione in 15 mM HEPES buffer (pH 7.7) after applying on its surface a drop of a solution of glutathione (0.5 mM) and ferrous iron (1 mM) in the same buffer of volume 10 μL was detected. At regular intervals, the picture of autowaves changed in time intervals of 0.4–0.6 s over a period of 3 s after the application of the drop onto the solution. Then the structured picture of the distribution of DNIC dissipated, followed by a uniform green coloring of the solution caused by a uniform distribution of DNIC in it. It is assumed that the formation of autowaves is a consequence of the autooscillatory mode of the existence of a chemical system formed in a mixture of NO, low-molecular-weight thiols, and ferrous iron ions. DNIC with thiolate ligands and S-nitrosothiols arising in this system have a capacity for interconversion, and it is this process that may underlie the autooscillatory, autowave mode of functioning of the system. It is not ruled out that the existence of this system in cells and tissues of living organisms may provide the spatial and temporal organization of the regulation of the biological action of NO and its different endogenous compounds and derivatives.

  • Effects of dinitrosyl iron Complex with glutathione and its components on ischemic rat heart during reperfusion
    Biophysics, 2009
    Co-Authors: O. I. Pisarenko, A. A. Timoshin, V. S. Shulzhenko, I. M. Studneva, Yu. A. Pelogeikina, A. F. Vanin
    Abstract:

    The effects of a Complex of dinitrosyl iron with glutathione (DNIC-GS) lyophilized on dextran, its hydrolysis products (glutathione, nitrosoglutathione, dextran), as well as nitric oxide released from the drug on the energy metabolism and functional recovery of isolated perfused rat heart subjected to global ischemia and reperfusion have been studied. Infusion of 100 nM DNIC-GS after ischemia substantially enhanced the recovery of coronary flow, cardiac contractile and pump functions during reperfusion, with simultaneous preservation of myocardial high-energy phosphates and cell membrane integrity. It was shown by EPR that these effects were associated with transfer of Fe^+(NO^+)_2 groups from DNIC-GS to thiol-containing proteins of cardiomyocytes and coronary vessels. Combined infusion of 100 nM DNIC-GS and 25 μM 2-(phenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide, a nitric oxide scavenger, after ischemia profoundly reduced the metabolic and functional recovery of reperfused hearts. After postischemic administration of an equivalent amount of DNIC-GS hydrolysate (completely decomposed Complex), most of the indices did not differ from those in control or were significantly lower. Thus, inclusion of Fe^+(NO^+)_2 groups into myocardial tissue and spontaneous release of nitric oxide trigger the protective mechanisms in the ischemic heart.

  • Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli
    Russian Journal of Genetics, 2008
    Co-Authors: S. V. Vasilieva, V. D. Mikoyan, E. Yu. Moshkovskaya, A. S. Terekhov, A. F. Vanin
    Abstract:

    In the exponentially growing E. coli cells we have described in 2005 a new fundamental genetic phenomenon, namely quasi-adaptive response to alkylating compounds, “quasi-Ada”. Phenotypic expression of “quasi-Ada” is similar to the true Ada response, however in contrast it develops in the course of pretreatment of the cells by sublethal dose of non-alkylating agent, an NO-containing dinitrosyl iron Complex with glutathione (DNIC_glu). To reveal the mechanisms of quasi-adaptation and its association with the function of the regulatory protein Ada here we used a unique property of dual gene expression regulation of aidB1 gene, a part of Ada-regulon, namely its relative independence from Ada protein in anaerobic conditions. Based on the results of aidB1 gene expression analysis an EPR spectra of E.coli MV2176 cells ( aidB1::lacZ ) in aerobic and anaerobic conditions after the corresponding treatments we concluded that the function and the spatial structure of ^meAda and [(Cys^−)_2Fe^+(NO^+)_2]Ada are identical and thus the nitrosylated protein represents an Ada regulon genes expression regulator during quasi-adaptation development.

  • Genetic Signal Transduction by Nitrosyl-Iron Complexes in Escherichia coli
    Biochemistry (Moscow), 2004
    Co-Authors: S. V. Vasilieva, Nataliya A Sanina, S M Aldoshin, E. Yu. Moshkovskaya, A. F. Vanin
    Abstract:

    Nitrosyl-iron Complexes used as aqueous preparations of binuclear Dinitrosyl-Iron Complex with glutathione (DNIC_glu), initially polycrystalline preparations of binuclear tetranitrosyl-iron Complex with thiosulfate (TNIC_thio), and also binuclear tetranitrosyl-iron Complex with aminotriazole (TNIC_atria) and mononuclear Dinitrosyl-Iron Complex with triazole (DNIC_tria) in the concentration to 0.1 mM activated expression of the soxS and sfiA genes in Escherichia coli . Higher concentrations of polycrystalline preparations of low stability in aqueous solutions were cytotoxic, whereas DNIC_glu, which is more stable in water (up to two days), increased the gene expression on increase in its concentration to 0.5 mM. The iron chelating agent o -phenanthroline completely inhibited the gene expression induced by all compounds studied. The genetic signal transduction seemed to be realized not by nitric oxide molecules and/or iron ions released in solutions but directly by the Complexes themselves, which activate transcriptional proteins by transfer onto them of nitrosyl-iron groups [Fe^+(NO^+)_2].

Anatoly F. Vanin - One of the best experts on this subject based on the ideXlab platform.

  • EPR Characterization of Dinitrosyl Iron Complexes with Thiol-Containing Ligands as an Approach to Their Identification in Biological Objects: An Overview
    Cell Biochemistry and Biophysics, 2018
    Co-Authors: Anatoly F. Vanin
    Abstract:

    The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron Complexes with thiol-containing ligands in various animal and bacterial cells. These Complexes are formed in biological objects in the paramagnetic (electron paramagnetic resonance-active) mononuclear and diamagnetic (electron paramagnetic resonance-silent) binuclear forms and control the activity of nitrogen monoxide, one of the most universal regulators of metabolic processes in the organism. The analysis of electronic and spatial structures of dinitrosyl iron Complex sheds additional light on the mechanism whereby dinitrosyl iron Complex with thiol-containing ligands function in human and animal cells as donors of nitrogen monoxide and its ionized form, viz., nitrosonium ions (NO^+).

  • The antitumor activity of the S-nitrosoglutathione and dinitrosyl iron Complex with glutathione: Comparative studies
    Biophysics, 2015
    Co-Authors: Anatoly F. Vanin, L. A. Ostrovskaya, D. B. Korman, L. N. Kubrina, R. R. Borodulin, M. M. Fomina, N. V. Bluchterova, V. A. Rykova, A. A. Timoshin
    Abstract:

    The antitumor activity of the binuclear form of dinitrosyl iron Complexes with glutathione against Lewis lung carcinoma was found earlier with intraperitoneal administration of the Complexes. This activity was also observed when this preparation was injected subcutaneously. The Complex inhibited the tumor growth by 43% upon subcutaneous injection at a daily dose of 100 µM/kg (as calculated per one iron atom in the binuclear dinitrosyl iron Complex) for 10 or 15 days. The effect was observed during the first 2 weeks after tumor transplantation. After this, the tumors began to grow at a rate that was equal to or even higher than that for the control animals. The mean survival time for the treated mice exceeded the control values by 30%. Binuclear dinitrosyl iron Complexes were also effective against Ca-755 adenocarcinoma with intraperitoneal administration. In this case, however, the mean survival time for the treated animals only increased by 7%. It was also shown that S-nitrosoglutathione inhibited the growth of Lewis lung carcinoma and Ca-755 adenocarcinoma by 70 and 90%, respectively. However, in contrast to binuclear dinitrosyl iron Complexes, the antitumor effect of S-nitrosoglutathione decreased with an increase in the daily dose of the compound from 200 to 400 µM/kg. The initial antitumor effect of binuclear dinitrosyl iron Complexes and S-nitrosoglutathione is suggested to be due to NO that is released from both compounds. The subsequent suppression of the effect is caused by the activation of antinitrosative and antioxidant defense systems in tumors.

  • Anti-Tumour Activity of Dinitrosyl Iron Complex with Glutathione and S-Nitrosoglutathione Preparations: Comparative Studies
    Biofizika, 2015
    Co-Authors: Anatoly F. Vanin, L. A. Ostrovskaya, D. B. Korman, L. N. Kubrina, R. R. Borodulin, M. M. Fomina, N. V. Bluchterova, Rykova Va, A. A. Timoshin
    Abstract:

    : The anti-tumor activity of the binuclear form of dinitrosyl iron Complexes with glutathione against Lewis lung carcinoma, found earlier upon intraperitoneal administration of the Complexes, was also observed when this preparation was injected subcutaneously. A 100 μM/kg subcutaneous dose of the Complex being used daily (as calculated per one iron atom in binuclear dinitrosyl iron Complexes) for 10 or 15 days, inhibited the tumor growth by 43%. The effect was observed during the first two weeks after tumor transplantation. After that, the tumors began to grow at the rate equal to or even higher than that one for control animals. The mean survival time for treated mice exceeded the control values by 30%. Binuclear dinitrosyl iron Complexes administered intraperitoneally was also effective against Ca-755 adenocarcinoma. However, in this case the mean survival time for treated animals increased only by 7%. The anti-tumor activity of S-nitrosoglutathione against Lewis lung carcinoma growth inhibition by 70% and Ca-755 adenocarcinoma growth inhibition by 90% was also shown. However, unlike binuclear dinitrosyl iron Complexes the anti-tumor effect of S-nitrosoglutathione decreased when a daily dose of the compound increased (from 200 to 400 μM/kg) The initial anti-tumor effect of binuclear dinitrosyl iron Complexes and S-nitrosoglutathione is suggested to be due to NO released from both compounds. A subsequent suppression of the effect is determined by the development of anti-nitrosative and anti-oxidant defense systems in tumors.

  • Transport of dinitrosyl iron Complexes into animal lungs
    Biofizika, 2015
    Co-Authors: G. N. Mojokina, V. D. Mikoyan, N. A. Elistratova, Anatoly F. Vanin
    Abstract:

    : Effective accumulation of binuclear dinitrosyl iron Complexes with glutathione was shown after a subcutaneous para lymphatic injection of an aqueous solution of a Dinitrosyl-Iron Complex into animal lung tissue at a single-dose of 2 micromoles per kilogram two times a day with a 2-h interval. Two hours later after the administration was repeated the concentration of these Complexes was 16 micromoles per kilogram of tissue dropping down for the last two hours to 7 micromoles per kilogram of tissue. At one dose injection of binuclear dinitrosyl iron Complexes with glutathione their concentration in 2 and 4 hours was two times lower than in the previous experiments. Presumably at the obtained concentration of dinitrosyl iron Complexes a bactericidal effect in lungs can be observed against mycobacterium tuberculosis and rapidly proliferating lung tumors.

  • EPR Characterization of Mononuclear Dinitrosyl Iron Complex with Persulfide as a New Representative of Dinitrosyl Iron Complexes in Biological Systems: an Overview
    Applied Magnetic Resonance, 2014
    Co-Authors: Anatoly F. Vanin, Svetlana V. Vasilyeva, Darya A. Streltsova, Vasak D. Mikoyan
    Abstract:

    Some recent data on the presence of mononuclear dinitrosyl iron Complexes (M-DNIC) with persulfide (R-S-S^−) ligands with a characteristic electron paramagnetic resonance signal at g _⊥ = 2.35 and g _|| = 2.02 ( g _aver. = 2.03) in biological systems (e.g., Escherichia coli cells and isolated iron–sulfur proteins) are reviewed. The generation of M-DNIC is controlled by inorganic sulfur (sulfide, S^2−) whose binding to thiols gives persulfides. It is suggested that enhanced production of inorganic sulfur is a result of destruction of active centers of iron–sulfur proteins in the presence of NO or NO-containing compounds. Dinitrosyl iron Complexes with thiol-containing ligands are the most active participants in this process. Inorganic sulfur may appear in biological systems during the synthesis or resynthesis of active centers of iron–sulfur proteins in response to activation of cysteine desulfurase, the key enzyme in sulfide synthesis from cysteine.

S M Aldoshin - One of the best experts on this subject based on the ideXlab platform.

  • Anticancer Activity of Dinitrosyl Iron Complex (NO Donor) on the Multiple Myeloma Cells.
    Doklady Biochemistry and Biophysics, 2019
    Co-Authors: N. P. Akentieva, Nataliya A Sanina, T. R. Prichodchenko, A. R. Gizatullin, N. I. Shkondina, S. S. Shushanov, Tatyana S. Stupina, S M Aldoshin
    Abstract:

    The results of a study of the effect of a mononuclear dinitrosyl iron Complex (DNIC7) with functional sulfur-containing ligands (NO donor) on the cell viability of multiple myeloma cells are presented. It has been shown that DNIC7 decreased cell viability and inhibited the proliferation of cells of multiple myeloma, i.e. possesses cytotoxic properties. Fluorescent analysis revealed that the DNIC7 compound lowers the level of intracellular glutathione and increases the level of reactive oxygen species in cells of multiple myeloma. It is assumed that DNIC7 has the therapeutic potential for the treatment of cancer.

  • Anti-cancer activity of dinitrosyl iron Complex (no donor) on the multiple myeloma cells
    Доклады Академии наук, 2019
    Co-Authors: N. P. Akentieva, Nataliya A Sanina, T. R. Prichodchenko, A. R. Gizatullin, N. I. Shkondina, S. S. Shushanov, Tatyana S. Stupina, S M Aldoshin
    Abstract:

    The results of a study of the effect of a mononuclear dinitrosyl iron Complex (DNIC7) with functional sulfur-containing ligands (NO donor) on the cell viability of multiple myeloma cells are presented. It has been shown that DNIC7 decreased cell viability and inhibited the proliferation of cells of multiple myeloma, i.e. possesses cytotoxic properties. Fluorescent analysis revealed that the DNIC7 compound lowers the level of intracellular glutathione and increases the level of reactive oxygen species in cells of multiple myeloma. It is assumed that DNIC7 has the therapeutic potential for the treatment of cancer.

  • molecular and crystal structure of a cationic dinitrosyl iron Complex with 1 3 dimethylthiourea
    Journal of Structural Chemistry, 2017
    Co-Authors: Yu N Shmatko, Denis V. Korchagin, Gennadiy V Shilov, Nataliya A Sanina, S M Aldoshin
    Abstract:

    A new dinitrosyl iron Complex of the composition [Fe(SC(NHCH3)2)2(NO)2]Cl (I) is obtained by direct nitrosylation of ferrous sulfate and a hydrochloric acid solution of 1,3-dimethylthiourea. The characteristic features of the molecular and crystal structure of Complex I is determined by single crystal X-ray diffraction analysis.

  • Molecular and electronic structure and IR spectra of mononuclear dinitrosyl iron Complex Fe(SC_2H_3N_3)(SC_2H_2N_3)(NO)_2]: a theoretical study
    Russian Chemical Bulletin, 2007
    Co-Authors: A. F. Shestakov, Nataliya A Sanina, Yu. M. Shul’ga, N. S. Emel’yanova, S M Aldoshin
    Abstract:

    The molecular and electronic structures of different isomers of a mononuclear dinitrosyl iron Complex [Fe(SC_2H_3N_3)(SC_2H_2N_3)(NO)_2] were calculated by the B3LYP and PBE density functional methods. Both theoretical approaches provide good agreement between the calculated and experimental geometry of the lowest-lying isomer (bond lengths differ by 0.02–0.04 Å and bond angles differ by 2–3° in terms of root-mean square values). A feature of the Complex is an intramolecular hydrogen bond N-H...N between the thiolate and thione ligands, which causes equalization of the Fe-S and S-C bond lengths. The ground state of the system has a spin of 1/2 and exists at antiparallel orientation of the spin ( S = 3/2) of the Fe atom with formal electron configuration d^7 and two local spins ( S = 1/2) of the NO ligands. Although each NO group has a small negative charge, which is mainly localized on the O atom, the Fe-NO bond can be treated as similar to homeopolar one. This corresponds to the effective trivalent state of Fe with an oxidation state of 1+. Both theoretical methods correctly reproduce the experimental structure of the IR spectrum, but the PBE functional provides a better description of absolute positions of spectral lines, whereas the B3LYP functional gives a somewhat better description of the relative intensities of spectral components. In spite of similar geometric parameters of coordination of two NO groups, the splitting between the NO stretching bands is rather large (58 cm^−1); this value is satisfactorily reproduced in theoretical calculations. A strong intramolecular hydrogen bond causes a large frequency shift of the N-H stretching vibrations corresponding to a broad absorption band in the region 2300–2600 cm^−1.

  • Protein-bound Dinitrosyl-Iron Complexes appearing in blood of rabbit added with a low-molecular Dinitrosyl-Iron Complex: EPR studies.
    Nitric Oxide, 2006
    Co-Authors: Alexander A. Timoshin, Nataliya A Sanina, S M Aldoshin, Anatoly F. Vanin, Enno K. Ruuge, Tsvetina R. Orlova, Evgeny I. Chazov
    Abstract:

    Abstract The formation of protein-bound dinitrosyl–iron Complexes (DNIC) in blood plasma and packed red cell fraction has been demonstrated by the EPR method in the experiments on rabbits which were i/v injected with the low-molecular DNIC with thiosulphate. This formation was ensured by transfer of Fe + (NO + ) 2 moieties from low-molecular DNIC onto serum albumin or hemoglobin molecules. Protein-bound DNICs appeared immediately after low-molecular DNIC injection followed with gradually decreasing their amounts. The Complexes could be detected by EPR technique during more than two days. The addition of water-soluble NO scavenger, the iron Complex with N -methyl- d -glucamine dithiocarbamate (MGD) resulted in decomposition of a part of protein-bound DNICs and in effective excretion of secondary products (mainly mononitrosyl–iron Complexes with MGD) from the blood flow.

V. D. Mikoyan - One of the best experts on this subject based on the ideXlab platform.

  • Transport of dinitrosyl iron Complexes into animal lungs
    Biofizika, 2015
    Co-Authors: G. N. Mojokina, V. D. Mikoyan, N. A. Elistratova, Anatoly F. Vanin
    Abstract:

    : Effective accumulation of binuclear dinitrosyl iron Complexes with glutathione was shown after a subcutaneous para lymphatic injection of an aqueous solution of a Dinitrosyl-Iron Complex into animal lung tissue at a single-dose of 2 micromoles per kilogram two times a day with a 2-h interval. Two hours later after the administration was repeated the concentration of these Complexes was 16 micromoles per kilogram of tissue dropping down for the last two hours to 7 micromoles per kilogram of tissue. At one dose injection of binuclear dinitrosyl iron Complexes with glutathione their concentration in 2 and 4 hours was two times lower than in the previous experiments. Presumably at the obtained concentration of dinitrosyl iron Complexes a bactericidal effect in lungs can be observed against mycobacterium tuberculosis and rapidly proliferating lung tumors.

  • Detection of autowave distribution of the concentration of a dinitrosyl iron Complex with glutathione formed in an aqueous solution of S-nitrosoglutathione after addition of a mixture of glutathione and ferrous iron
    Biophysics, 2010
    Co-Authors: A. F. Vanin, V. D. Mikoyan, L. N. Kubrina
    Abstract:

    The formation of dark green concentric autowaves of the distribution of the concentration of dinitrosyl iron Complex (DNIC) with glutathione in a thin (0.3 mm thick) layer of 0.5 M solution of S-nitrosoglutathione in 15 mM HEPES buffer (pH 7.7) after applying on its surface a drop of a solution of glutathione (0.5 mM) and ferrous iron (1 mM) in the same buffer of volume 10 μL was detected. At regular intervals, the picture of autowaves changed in time intervals of 0.4–0.6 s over a period of 3 s after the application of the drop onto the solution. Then the structured picture of the distribution of DNIC dissipated, followed by a uniform green coloring of the solution caused by a uniform distribution of DNIC in it. It is assumed that the formation of autowaves is a consequence of the autooscillatory mode of the existence of a chemical system formed in a mixture of NO, low-molecular-weight thiols, and ferrous iron ions. DNIC with thiolate ligands and S-nitrosothiols arising in this system have a capacity for interconversion, and it is this process that may underlie the autooscillatory, autowave mode of functioning of the system. It is not ruled out that the existence of this system in cells and tissues of living organisms may provide the spatial and temporal organization of the regulation of the biological action of NO and its different endogenous compounds and derivatives.

  • Quasi-adaptive response to alkylating agents and Ada-protein functions in Escherichia coli
    Russian Journal of Genetics, 2008
    Co-Authors: S. V. Vasilieva, V. D. Mikoyan, E. Yu. Moshkovskaya, A. S. Terekhov, A. F. Vanin
    Abstract:

    In the exponentially growing E. coli cells we have described in 2005 a new fundamental genetic phenomenon, namely quasi-adaptive response to alkylating compounds, “quasi-Ada”. Phenotypic expression of “quasi-Ada” is similar to the true Ada response, however in contrast it develops in the course of pretreatment of the cells by sublethal dose of non-alkylating agent, an NO-containing dinitrosyl iron Complex with glutathione (DNIC_glu). To reveal the mechanisms of quasi-adaptation and its association with the function of the regulatory protein Ada here we used a unique property of dual gene expression regulation of aidB1 gene, a part of Ada-regulon, namely its relative independence from Ada protein in anaerobic conditions. Based on the results of aidB1 gene expression analysis an EPR spectra of E.coli MV2176 cells ( aidB1::lacZ ) in aerobic and anaerobic conditions after the corresponding treatments we concluded that the function and the spatial structure of ^meAda and [(Cys^−)_2Fe^+(NO^+)_2]Ada are identical and thus the nitrosylated protein represents an Ada regulon genes expression regulator during quasi-adaptation development.

  • Hypotensive effect and tissue distribution of the dinitrosyl iron Complexes, a nitric oxide donor
    Bulletin of Experimental Biology and Medicine, 1998
    Co-Authors: E. B. Manukhina, L. N. Kubrina, V. D. Mikoyan, I. Yu. Malyshev, E. B. Malenyuk, T. A. Zenina, D. A. Podkidyshev, A. F. Vanin
    Abstract:

    Hypotensive effect of the dinitrosyl iron Complexes, an NO donor, is compared with distribution of these Complexes in organs and tissues after their intravenous administration to wakeful animals. Hypotensive effect of iron Complexes depended on dose and postinjection time. There was a strong correlation between hypotensive effect and the content of dinitrosyl iron Complex in the studied organs. Effective dose of dinitrosyl iron Complexes that did not provoke adverse effects was about 200 mg/kg. This preparation is a prospective source of NO to treat and prevent pathological states related to NO deficiency.

Nataliya A Sanina - One of the best experts on this subject based on the ideXlab platform.

  • Anticancer Activity of Dinitrosyl Iron Complex (NO Donor) on the Multiple Myeloma Cells.
    Doklady Biochemistry and Biophysics, 2019
    Co-Authors: N. P. Akentieva, Nataliya A Sanina, T. R. Prichodchenko, A. R. Gizatullin, N. I. Shkondina, S. S. Shushanov, Tatyana S. Stupina, S M Aldoshin
    Abstract:

    The results of a study of the effect of a mononuclear dinitrosyl iron Complex (DNIC7) with functional sulfur-containing ligands (NO donor) on the cell viability of multiple myeloma cells are presented. It has been shown that DNIC7 decreased cell viability and inhibited the proliferation of cells of multiple myeloma, i.e. possesses cytotoxic properties. Fluorescent analysis revealed that the DNIC7 compound lowers the level of intracellular glutathione and increases the level of reactive oxygen species in cells of multiple myeloma. It is assumed that DNIC7 has the therapeutic potential for the treatment of cancer.

  • Anti-cancer activity of dinitrosyl iron Complex (no donor) on the multiple myeloma cells
    Доклады Академии наук, 2019
    Co-Authors: N. P. Akentieva, Nataliya A Sanina, T. R. Prichodchenko, A. R. Gizatullin, N. I. Shkondina, S. S. Shushanov, Tatyana S. Stupina, S M Aldoshin
    Abstract:

    The results of a study of the effect of a mononuclear dinitrosyl iron Complex (DNIC7) with functional sulfur-containing ligands (NO donor) on the cell viability of multiple myeloma cells are presented. It has been shown that DNIC7 decreased cell viability and inhibited the proliferation of cells of multiple myeloma, i.e. possesses cytotoxic properties. Fluorescent analysis revealed that the DNIC7 compound lowers the level of intracellular glutathione and increases the level of reactive oxygen species in cells of multiple myeloma. It is assumed that DNIC7 has the therapeutic potential for the treatment of cancer.

  • molecular and crystal structure of a cationic dinitrosyl iron Complex with 1 3 dimethylthiourea
    Journal of Structural Chemistry, 2017
    Co-Authors: Yu N Shmatko, Denis V. Korchagin, Gennadiy V Shilov, Nataliya A Sanina, S M Aldoshin
    Abstract:

    A new dinitrosyl iron Complex of the composition [Fe(SC(NHCH3)2)2(NO)2]Cl (I) is obtained by direct nitrosylation of ferrous sulfate and a hydrochloric acid solution of 1,3-dimethylthiourea. The characteristic features of the molecular and crystal structure of Complex I is determined by single crystal X-ray diffraction analysis.

  • Molecular and electronic structure and IR spectra of mononuclear dinitrosyl iron Complex Fe(SC_2H_3N_3)(SC_2H_2N_3)(NO)_2]: a theoretical study
    Russian Chemical Bulletin, 2007
    Co-Authors: A. F. Shestakov, Nataliya A Sanina, Yu. M. Shul’ga, N. S. Emel’yanova, S M Aldoshin
    Abstract:

    The molecular and electronic structures of different isomers of a mononuclear dinitrosyl iron Complex [Fe(SC_2H_3N_3)(SC_2H_2N_3)(NO)_2] were calculated by the B3LYP and PBE density functional methods. Both theoretical approaches provide good agreement between the calculated and experimental geometry of the lowest-lying isomer (bond lengths differ by 0.02–0.04 Å and bond angles differ by 2–3° in terms of root-mean square values). A feature of the Complex is an intramolecular hydrogen bond N-H...N between the thiolate and thione ligands, which causes equalization of the Fe-S and S-C bond lengths. The ground state of the system has a spin of 1/2 and exists at antiparallel orientation of the spin ( S = 3/2) of the Fe atom with formal electron configuration d^7 and two local spins ( S = 1/2) of the NO ligands. Although each NO group has a small negative charge, which is mainly localized on the O atom, the Fe-NO bond can be treated as similar to homeopolar one. This corresponds to the effective trivalent state of Fe with an oxidation state of 1+. Both theoretical methods correctly reproduce the experimental structure of the IR spectrum, but the PBE functional provides a better description of absolute positions of spectral lines, whereas the B3LYP functional gives a somewhat better description of the relative intensities of spectral components. In spite of similar geometric parameters of coordination of two NO groups, the splitting between the NO stretching bands is rather large (58 cm^−1); this value is satisfactorily reproduced in theoretical calculations. A strong intramolecular hydrogen bond causes a large frequency shift of the N-H stretching vibrations corresponding to a broad absorption band in the region 2300–2600 cm^−1.

  • Protein-bound Dinitrosyl-Iron Complexes appearing in blood of rabbit added with a low-molecular Dinitrosyl-Iron Complex: EPR studies.
    Nitric Oxide, 2006
    Co-Authors: Alexander A. Timoshin, Nataliya A Sanina, S M Aldoshin, Anatoly F. Vanin, Enno K. Ruuge, Tsvetina R. Orlova, Evgeny I. Chazov
    Abstract:

    Abstract The formation of protein-bound dinitrosyl–iron Complexes (DNIC) in blood plasma and packed red cell fraction has been demonstrated by the EPR method in the experiments on rabbits which were i/v injected with the low-molecular DNIC with thiosulphate. This formation was ensured by transfer of Fe + (NO + ) 2 moieties from low-molecular DNIC onto serum albumin or hemoglobin molecules. Protein-bound DNICs appeared immediately after low-molecular DNIC injection followed with gradually decreasing their amounts. The Complexes could be detected by EPR technique during more than two days. The addition of water-soluble NO scavenger, the iron Complex with N -methyl- d -glucamine dithiocarbamate (MGD) resulted in decomposition of a part of protein-bound DNICs and in effective excretion of secondary products (mainly mononitrosyl–iron Complexes with MGD) from the blood flow.