The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
L C Comeau - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium
Lipids, 1998Co-Authors: Aida Ibrik, Nathalie Rugani, Louis Sarda, Henri Chahinian, L C ComeauAbstract:An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium . The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and Dioctanoin) and methyl esters. It is stable at 35°C for 60 min and has maximal activity in a pH range of 8–10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.
Aida Ibrik - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium
Lipids, 1998Co-Authors: Aida Ibrik, Nathalie Rugani, Louis Sarda, Henri Chahinian, L C ComeauAbstract:An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium . The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and Dioctanoin) and methyl esters. It is stable at 35°C for 60 min and has maximal activity in a pH range of 8–10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.
Nathalie Rugani - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium
Lipids, 1998Co-Authors: Aida Ibrik, Nathalie Rugani, Louis Sarda, Henri Chahinian, L C ComeauAbstract:An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium . The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and Dioctanoin) and methyl esters. It is stable at 35°C for 60 min and has maximal activity in a pH range of 8–10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.
Louis Sarda - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium
Lipids, 1998Co-Authors: Aida Ibrik, Nathalie Rugani, Louis Sarda, Henri Chahinian, L C ComeauAbstract:An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium . The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and Dioctanoin) and methyl esters. It is stable at 35°C for 60 min and has maximal activity in a pH range of 8–10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.
Henri Chahinian - One of the best experts on this subject based on the ideXlab platform.
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Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium
Lipids, 1998Co-Authors: Aida Ibrik, Nathalie Rugani, Louis Sarda, Henri Chahinian, L C ComeauAbstract:An extracellular lipase, active on water-insoluble triacylglycerols, has been isolated from Penicillium cyclopium . The purified enzyme has a molecular mass of 29 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis. It hydrolyzes emulsions of tributyrin, trioctanoin, and olive oil at the same rate as pancreatic lipase and shows very low activity against partial acylglycerols (monooctanoin and Dioctanoin) and methyl esters. It is stable at 35°C for 60 min and has maximal activity in a pH range of 8–10. Hydrolysis of triacylglycerols by P. cyclopium lipase is inhibited by detergents such as Triton X-100. Comparison of the sequence of the 20 first amino acid residues of P. cyclopium triacylglycerol lipase with other Penicillium lipases indicates a high homology with previously characterized lipases produced by P. expansum and P. solitum which are enzymes of comparable size and substrate specificity. Conversely, homology between P. cyclopium lipase and P. simplicissimum lipase, a nonspecific lipolytic enzyme, is low. Penicillium cyclopium triacylglycerol lipase shows no homology with P. camembertii lipase which is specific to monoacylglycerol and diacylglycerol.