The Experts below are selected from a list of 7389 Experts worldwide ranked by ideXlab platform
Markus Pfenninger - One of the best experts on this subject based on the ideXlab platform.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2020Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius9 genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species, and the respective recombination rate among the first insect recombination rates at all. The genome assembly and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in particular. The estimated recombination rate will help evolutionary biologists gaining a better understanding of commonalities and differences of genomic patterns in insects.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2019Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Abstract Background Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Findings Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius’ genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species and the respective recombination rate as one of the first insect recombination rates at all. Conclusions The genome and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in detail. The estimated recombination rate will help evolutionary biologist gain a better understanding of commonalities and differences of genomic patterns in insects.
Hanno Schmidt - One of the best experts on this subject based on the ideXlab platform.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2020Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius9 genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species, and the respective recombination rate among the first insect recombination rates at all. The genome assembly and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in particular. The estimated recombination rate will help evolutionary biologists gaining a better understanding of commonalities and differences of genomic patterns in insects.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2019Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Abstract Background Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Findings Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius’ genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species and the respective recombination rate as one of the first insect recombination rates at all. Conclusions The genome and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in detail. The estimated recombination rate will help evolutionary biologist gain a better understanding of commonalities and differences of genomic patterns in insects.
Lynn M Riddiford - One of the best experts on this subject based on the ideXlab platform.
-
Dev Genes Evol (2000) 210:358–372 © Springer-Verlag 2000 ORIGINAL ARTICLE
2013Co-Authors: Jason Hodin, Lynn M RiddifordAbstract:Parallel alterations in the timing of ovarian Ecdysone Receptor and Ultraspiracle expression characterize the independent evolution of larval reproduction in two species of gall midges (Diptera: Cecidomyiidae) Received: 30 July 1999 / Accepted: 23 Feburary 2000 Abstract Although most insects reproduce in the adult stage, facultative larval or pupal reproduction (paedogenesis) has evolved at least six times indepently in insects, twice in gall midges of the family Cecidomyiidae (Diptera). Paedogenesis in gall midges involves the precocious growth and differentiation of the ovary in an otherwise larval form. We have previously shown that the timing of expression of the Ecdysone Receptor (EcR) and Ultraspiracle (USP), the two proteins that constitute the functional receptor for the steroid hormone 20-hydroxyecdysone, regulates the timing and progression of ovarian differentiation in Drosophila melanogaster (Diptera: Drosophilidae). Here we test the hypothesis that precocious activation of EcR and USP in the ovaries of paedogenetic gall midges allows for precocious ovarian differentiation. Using monoclonal antibodies directed against insect EcR and USP proteins, we first show that when these gall midges are reared under conditions that promote typical, metamorphic development, upregulation of EcR and USP occurs in the final larval stage. By contrast, in the paedogenetic life cycle, EcR and USP are up-regulated early in the first larval stage. A similar pattern is seen for two independently-evolved paedogenetic gall midges, Heteropeza pygmaea and Mycophila speyeri. We discuss our results in the context of developmental constraints on the evolution of paedogenesis in Dipteran insects
-
parallel alterations in the timing of ovarian ecdysone receptor and ultraspiracle expression characterize the independent evolution of larval reproduction in two species of gall midges diptera cecidomyiidae
2000Co-Authors: Jason Hodin, Lynn M RiddifordAbstract:Although most insects reproduce in the adult stage, facultative larval or pupal reproduction (paedogenesis) has evolved at least six times indepently in insects, twice in gall midges of the family Cecidomyiidae (Diptera). Paedogenesis in gall midges involves the precocious growth and differentiation of the ovary in an otherwise larval form. We have previously shown that the timing of expression of the Ecdysone Receptor (EcR) and Ultraspiracle (USP), the two proteins that constitute the functional receptor for the steroid hormone 20-hydroxyecdysone, regulates the timing and progression of ovarian differentiation in Drosophila melanogaster (Diptera: Drosophilidae). Here we test the hypothesis that precocious activation of EcR and USP in the ovaries of paedogenetic gall midges allows for precocious ovarian differentiation. Using monoclonal antibodies directed against insect EcR and USP proteins, we first show that when these gall midges are reared under conditions that promote typical, metamorphic development, up- regulation of EcR and USP occurs in the final larval stage. By contrast, in the paedogenetic life cycle, EcR and USP are up-regulated early in the first larval stage. A similar pattern is seen for two independently-evolved paedogenetic gall midges, Heteropeza pygmaea and Mycophila speyeri. We discuss our results in the context of developmental constraints on the evolution of paedogenesis in Dipteran insects.
-
the sequence of locusta rxr homologous to drosophila ultraspiracle and its evolutionary implications
1999Co-Authors: David C Hayward, Lynn M Riddiford, Michael J Bastiani, John W H Trueman, James W Truman, Eldon E BallAbstract:The cellular response to steroid hormones is mediated by nuclear receptors which act by regulating transcription. In Drosophila melanogaster, the receptor for the insect molting hormone, 20-hydroxyecdysone, is a heterodimer composed of the Ecdysone Receptor and Ultraspiracle (USP) proteins. The DNA binding domains of arthropod USPs and their vertebrate homologs, the retinoid X receptor (RXR) family, are highly conserved. The ligand binding domain sequences, however, divide into two distinct groups. One group consists of sequences from members of the holometabolous higher insect orders Diptera and Lepidoptera, the other of sequences from vertebrates, a crab and a tick. We here report the sequence of an RXR/USP from the hemimetabolous orthopteran, Locusta migratoria. The locust RXR/USP ligand binding domain clearly falls in the vertebrate-crab-tick rather than the Dipteran-lepidopteran group. The reason for the evolutionarily abrupt divergence of the Dipteran and lepidopteran sequences is unknown, but it could be a change in the type of ligand bound or the loss of ligand altogether.
B A Foote - One of the best experts on this subject based on the ideXlab platform.
-
biology and ecology of higher diptera from freshwater wetlands
2002Co-Authors: Joe B Keiper, William E Walton, B A FooteAbstract:Although studies of freshwater entomofauna frequently do not include the biodiversity and ecological roles of higher Diptera, cyclorraphous flies are often numerous and species rich in wetlands. Seventeen families are commonly found in freshwater wetlands, with Ephydridae, Chloropidae, Sciomyzidae, Sphaeroceridae, and Scathophagidae being among the most important in terms of population size and species richness. Difficulty with sampling cryptic larval habitats and species identification challenges may account for the exclusion of acalyptrate and other Dipterans from wetlands ecology studies. Large populations are facilitated by the high productivity of freshwater wetlands and the high intrinsic rate of increase characteristic of many species. Higher Dipterans exist in all freshwater wetland types, are microhabitat selective, and play significant roles in food webs. The varied strategies for food acquisition and patterns of spatial and temporal distribution limit ecological overlap among the higher Diptera.
-
biology and ecology of higher diptera from freshwater wetlands
2002Co-Authors: Joe B Keiper, William E Walton, B A FooteAbstract:▪ Abstract Although studies of freshwater entomofauna frequently do not include the biodiversity and ecological roles of higher Diptera, cyclorraphous flies are often numerous and species rich in wetlands. Seventeen families are commonly found in freshwater wetlands, with Ephydridae, Chloropidae, Sciomyzidae, Sphaeroceridae, and Scathophagidae being among the most important in terms of population size and species richness. Difficulty with sampling cryptic larval habitats and species identification challenges may account for the exclusion of acalyptrate and other Dipterans from wetlands ecology studies. Large populations are facilitated by the high productivity of freshwater wetlands and the high intrinsic rate of increase characteristic of many species. Higher Dipterans exist in all freshwater wetland types, are microhabitat selective, and play significant roles in food webs. The varied strategies for food acquisition and patterns of spatial and temporal distribution limit ecological overlap among the hig...
Soren Lukas Hellmann - One of the best experts on this subject based on the ideXlab platform.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2020Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius9 genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species, and the respective recombination rate among the first insect recombination rates at all. The genome assembly and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in particular. The estimated recombination rate will help evolutionary biologists gaining a better understanding of commonalities and differences of genomic patterns in insects.
-
a high quality genome assembly from short and long reads for the non biting midge chironomus riparius diptera
2019Co-Authors: Hanno Schmidt, Soren Lukas Hellmann, Ann-marie Waldvogel, Barbara Feldmeyer, Thomas Hankeln, Markus PfenningerAbstract:Abstract Background Chironomus riparius is of great importance as a study species in various fields like ecotoxicology, molecular genetics, developmental biology and ecology. However, only a fragmented draft genome exists to date, hindering the recent rush of population genomic studies in this species. Findings Making use of 50 NGS datasets, we present a hybrid genome assembly from short and long sequence reads that make C. riparius’ genome one of the most contiguous Dipteran genomes published, the first complete mitochondrial genome of the species and the respective recombination rate as one of the first insect recombination rates at all. Conclusions The genome and associated resources will be highly valuable to the broad community working with Dipterans in general and chironomids in detail. The estimated recombination rate will help evolutionary biologist gain a better understanding of commonalities and differences of genomic patterns in insects.