The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform

R. Larsen - One of the best experts on this subject based on the ideXlab platform.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia
    Acta Anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    BACKGROUND AND METHODS Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. RESULTS Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). CONCLUSIONS These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia.
    Acta anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • Prediction of recovery after ischemia. A microcalorimetric and biochemical study of rat liver tissue
    Thermochimica Acta, 1995
    Co-Authors: F. Bach, D. Singer, M. Bauer, H.j. Bretschneider, R. Larsen
    Abstract:

    Abstract Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting the recovery of the liver after ischemic damage is still an unresolved problem. During the ischemic storage of isolated rat livers at 25°C, tissue concentrations of high energy phosphates, glycogen and lactate were determined photometrically and, in comparison, the metabolic rate was measured continuously by Direct Calorimetry. In addition, interstitial pH was measured with microelectrodes. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25°C for 60, 120 and 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion, functional recovery as assessed by oxygen consumption and bile flow was determined. At the end of reperfusion, tissue samples were taken for biochemical analysis of adenine nucleotides and tissue lactate. Though the ATP concentration drops within 60 min of ischemia to 6.94% of the control value without further significant change, the metabolic rate measured continuously by Direct Calorimetry decreases in an exponential manner. According to the calorimetrically measured heat output, functional hepatic metabolism, as assessed by oxygen consumption, bile flow and restoration of high energy phosphates during reperfusion, recovered to an extent depending on the duration of ischemia. It is concluded that the metabolic rate of the ischemic rat liver, as determined continuously by Direct Calorimetry, is a good predictor for recovery after ischemic damage.

F. Bach - One of the best experts on this subject based on the ideXlab platform.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia
    Acta Anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    BACKGROUND AND METHODS Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. RESULTS Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). CONCLUSIONS These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia.
    Acta anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • Prediction of recovery after ischemia. A microcalorimetric and biochemical study of rat liver tissue
    Thermochimica Acta, 1995
    Co-Authors: F. Bach, D. Singer, M. Bauer, H.j. Bretschneider, R. Larsen
    Abstract:

    Abstract Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting the recovery of the liver after ischemic damage is still an unresolved problem. During the ischemic storage of isolated rat livers at 25°C, tissue concentrations of high energy phosphates, glycogen and lactate were determined photometrically and, in comparison, the metabolic rate was measured continuously by Direct Calorimetry. In addition, interstitial pH was measured with microelectrodes. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25°C for 60, 120 and 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion, functional recovery as assessed by oxygen consumption and bile flow was determined. At the end of reperfusion, tissue samples were taken for biochemical analysis of adenine nucleotides and tissue lactate. Though the ATP concentration drops within 60 min of ischemia to 6.94% of the control value without further significant change, the metabolic rate measured continuously by Direct Calorimetry decreases in an exponential manner. According to the calorimetrically measured heat output, functional hepatic metabolism, as assessed by oxygen consumption, bile flow and restoration of high energy phosphates during reperfusion, recovered to an extent depending on the duration of ischemia. It is concluded that the metabolic rate of the ischemic rat liver, as determined continuously by Direct Calorimetry, is a good predictor for recovery after ischemic damage.

M. Bauer - One of the best experts on this subject based on the ideXlab platform.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia
    Acta Anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    BACKGROUND AND METHODS Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. RESULTS Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). CONCLUSIONS These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia.
    Acta anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • Prediction of recovery after ischemia. A microcalorimetric and biochemical study of rat liver tissue
    Thermochimica Acta, 1995
    Co-Authors: F. Bach, D. Singer, M. Bauer, H.j. Bretschneider, R. Larsen
    Abstract:

    Abstract Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting the recovery of the liver after ischemic damage is still an unresolved problem. During the ischemic storage of isolated rat livers at 25°C, tissue concentrations of high energy phosphates, glycogen and lactate were determined photometrically and, in comparison, the metabolic rate was measured continuously by Direct Calorimetry. In addition, interstitial pH was measured with microelectrodes. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25°C for 60, 120 and 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion, functional recovery as assessed by oxygen consumption and bile flow was determined. At the end of reperfusion, tissue samples were taken for biochemical analysis of adenine nucleotides and tissue lactate. Though the ATP concentration drops within 60 min of ischemia to 6.94% of the control value without further significant change, the metabolic rate measured continuously by Direct Calorimetry decreases in an exponential manner. According to the calorimetrically measured heat output, functional hepatic metabolism, as assessed by oxygen consumption, bile flow and restoration of high energy phosphates during reperfusion, recovered to an extent depending on the duration of ischemia. It is concluded that the metabolic rate of the ischemic rat liver, as determined continuously by Direct Calorimetry, is a good predictor for recovery after ischemic damage.

D. Singer - One of the best experts on this subject based on the ideXlab platform.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia
    Acta Anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    BACKGROUND AND METHODS Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. RESULTS Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). CONCLUSIONS These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • High energy phosphates and Direct Calorimetry as predictive parameters for metabolic recovery of the rat liver following ischemia.
    Acta anaesthesiologica Scandinavica, 1996
    Co-Authors: F. Bach, D. Singer, A. Schmiedl, M. Bauer, R. Larsen
    Abstract:

    Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting recovery of the liver after ischemic damage is still an unresolved problem. During ischemic storage of isolated rat livers at 25 degrees C tissue concentrations of high energy phosphates and lactate were determined photometrically and interstitial pH was measured by glass electrodes. In comparison, the metabolic rate was measured continuously by Direct Calorimetry. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25 degrees C for 60, 120 or 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion functional recovery, as assessed by oxygen consumption and bile flow, was determined. At the end of reperfusion tissue samples were taken for biochemical analysis of adenine nucleotides. Furthermore, morphologic integrity was determined by electron microscopy. Whereas the ATP concentration drops within 60 min of ischemia to 6.9% of the control value without further significant change, the continuously measured metabolic rate as assessed by Direct Calorimetry decreases in an exponential manner. Accordingly, a better correlation of hepatocellular secretory function and calorimetrically measured heat output (r2 = 0.85; P < 0.001) was observed than with high energy phosphates (r2 = 0.56; P < 0.001). These data suggest that if the metabolism of the ischemic rat liver falls below a critical level, recovery is incomplete or impossible. Therefore, assessment of the global metabolic rate by Direct Calorimetry seems not only to be a very good predictor of recovery after ischemic damage but also a good tool in the laboratory for studies concerning the sequelae of ischemic metabolism and for improvement of tissue protection.

  • Prediction of recovery after ischemia. A microcalorimetric and biochemical study of rat liver tissue
    Thermochimica Acta, 1995
    Co-Authors: F. Bach, D. Singer, M. Bauer, H.j. Bretschneider, R. Larsen
    Abstract:

    Abstract Alteration of the hepatocellular function following ischemic damage may play a crucial role in the limited recovery after reperfusion. In spite of numerous efforts, finding a simple technique for predicting the recovery of the liver after ischemic damage is still an unresolved problem. During the ischemic storage of isolated rat livers at 25°C, tissue concentrations of high energy phosphates, glycogen and lactate were determined photometrically and, in comparison, the metabolic rate was measured continuously by Direct Calorimetry. In addition, interstitial pH was measured with microelectrodes. In a second series of experiments these results were compared with functional recovery after ischemia and reperfusion. Following ischemic storage at 25°C for 60, 120 and 240 min, the isolated livers were reperfused for 30 min in a non-recirculating system with a constant flow rate. During reperfusion, functional recovery as assessed by oxygen consumption and bile flow was determined. At the end of reperfusion, tissue samples were taken for biochemical analysis of adenine nucleotides and tissue lactate. Though the ATP concentration drops within 60 min of ischemia to 6.94% of the control value without further significant change, the metabolic rate measured continuously by Direct Calorimetry decreases in an exponential manner. According to the calorimetrically measured heat output, functional hepatic metabolism, as assessed by oxygen consumption, bile flow and restoration of high energy phosphates during reperfusion, recovered to an extent depending on the duration of ischemia. It is concluded that the metabolic rate of the ischemic rat liver, as determined continuously by Direct Calorimetry, is a good predictor for recovery after ischemic damage.

S. N. Gridchin - One of the best experts on this subject based on the ideXlab platform.