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C G M Kallenberg - One of the best experts on this subject based on the ideXlab platform.

  • measurement of increases in anti double stranded dna antibody levels as a predictor of Disease Exacerbation in systemic lupus erythematosus a long term prospective study
    Arthritis & Rheumatism, 1990
    Co-Authors: E J Ter Borg, G Horst, E J Hummel, P C Limburg, C G M Kallenberg
    Abstract:

    To evaluate the predictive power of changes in levels of antibodies to double-stranded DNA (anti-dsDNA) as a predictor of Disease Exacerbations in systemic lupus erythematosus (SLE), we performed a prospective study on 72 unselected patients with SLE (mean duration of study 18.5 months, range 6–35 months). Patients were seen at least once every 3 months, and Disease activity was scored according to a specific protocol. Plasma samples were obtained at least once every month and were assessed for anti-dsDNA antibody (by the Crithidia luciliae assay, an enzymelinked immunosorbent assay [ELISA], and the Farr assay) and for complement components C3 and C4. Twenty-seven of 33 Disease Exacerbations observed during the study period were accompanied by a positive test result for anti-dsDNA antibody (27 by the Farr assay, 19 by the C luciliae assay, and 23 by the ELISA). Twenty-four of these Exacerbations were preceded by a significant increase in anti-dsDNA antibody levels (23 by the Farr assay, 12 by the C luciliae assay, and 17 by the ELISA). The first observance of a significant increase in anti-dsDNA antibody levels preceded the Exacerbation by 8–10 weeks. Significant increases in anti-dsDNA antibody levels not followed by an Exacerbation were observed in 5 cases by the Farr assay, in 7 cases by the C luciliae assay, and in 3 cases by the ELISA; however, in 3 cases, 2 cases, and 1 case, respectively, these increases were followed by an increase in Disease activity that did not fulfill the criteria for an Exacerbation. Serial measurement of anti-dsDNA antibody levels was more sensitive for predicting Exacerbations than was measurement of C3 and/or C4 levels (P < 0.03). Serial assessment of anti-dsDNA antibody levels, especially by the Farr assay, is a sensitive and reasonably specific method for predicting Disease Exacerbations in SLE.

  • measurement of increases in anti double stranded dna antibody levels as a predictor of Disease Exacerbation in systemic lupus erythematosus a long term prospective study
    Arthritis & Rheumatism, 1990
    Co-Authors: E J Ter Borg, G Horst, E J Hummel, P C Limburg, C G M Kallenberg
    Abstract:

    : To evaluate the predictive power of changes in levels of antibodies to double-stranded DNA (anti-dsDNA) as a predictor of Disease Exacerbations in systemic lupus erythematosus (SLE), we performed a prospective study on 72 unselected patients with SLE (mean duration of study 18.5 months, range 6-35 months). Patients were seen at least once every 3 months, and Disease activity was scored according to a specific protocol. Plasma samples were obtained at least once every month and were assessed for anti-dsDNA antibody (by the Crithidia luciliae assay, an enzyme-linked immunosorbent assay [ELISA], and the Farr assay) and for complement components C3 and C4. Twenty-seven of 33 Disease Exacerbations observed during the study period were accompanied by a positive test result for anti-dsDNA antibody (27 by the Farr assay, 19 by the C luciliae assay, and 23 by the ELISA). Twenty-four of these Exacerbations were preceded by a significant increase in anti-dsDNA antibody levels (23 by the Farr assay, 12 by the C luciliae assay, and 17 by the ELISA). The first observance of a significant increase in anti-dsDNA antibody levels preceded the Exacerbation by 8-10 weeks. Significant increases in anti-dsDNA antibody levels not followed by an Exacerbation were observed in 5 cases by the Farr assay, in 7 cases by the C luciliae assay, and in 3 cases by the ELISA; however, in 3 cases, 2 cases, and 1 case, respectively, these increases were followed by an increase in Disease activity that did not fulfill the criteria for an Exacerbation. Serial measurement of anti-dsDNA antibody levels was more sensitive for predicting Exacerbations than was measurement of C3 and/or C4 levels (P less than 0.03). Serial assessment of anti-dsDNA antibody levels, especially by the Farr assay, is a sensitive and reasonably specific method for predicting Disease Exacerbations in SLE.

Yin Chen - One of the best experts on this subject based on the ideXlab platform.

  • rhinovirus induced major airway mucin production involves a novel tlr3 egfr dependent pathway
    American Journal of Respiratory Cell and Molecular Biology, 2009
    Co-Authors: Yuhua Zhao, Dongfang Yu, Yin Chen
    Abstract:

    Mucociliary clearance is a critical innate defense system responsible for clearing up invading pathogens including bacteria and virus. Although the right amount of mucus is good, excessive mucus causes airway obstruction and tends to precipitate Disease symptoms. Rhinovirus (RV) is a common cold virus that causes asthma and chronic obstructive pulmonary Disease Exacerbation. Mucus overproduction has been linked to the pathogenesis of RV-induced Diseases and Disease Exacerbations. However, the molecular mechanism is not clear. In this study, using one of the major airway mucin-MUC5AC as marker, we found that both major and minor groups of RV induced mucin production in primary human epithelial cells and cell line. RV1A (a minor group of RV) could induce mucous cell metaplasia in vivo. Viral replication was needed for RV-induced mucin expression, and this induction was also dependent on TLR3, suggesting the involvement of double-stranded (ds) RNA signaling. Indeed, dsRNA alone could also induce mucin expres...

  • Rhinovirus-Induced Major Airway Mucin Production Involves a Novel TLR3-EGFR–Dependent Pathway
    American Journal of Respiratory Cell and Molecular Biology, 2008
    Co-Authors: Yuhua Zhao, Dongfang Yu, Yin Chen
    Abstract:

    Mucociliary clearance is a critical innate defense system responsible for clearing up invading pathogens including bacteria and virus. Although the right amount of mucus is good, excessive mucus causes airway obstruction and tends to precipitate Disease symptoms. Rhinovirus (RV) is a common cold virus that causes asthma and chronic obstructive pulmonary Disease Exacerbation. Mucus overproduction has been linked to the pathogenesis of RV-induced Diseases and Disease Exacerbations. However, the molecular mechanism is not clear. In this study, using one of the major airway mucin-MUC5AC as marker, we found that both major and minor groups of RV induced mucin production in primary human epithelial cells and cell line. RV1A (a minor group of RV) could induce mucous cell metaplasia in vivo. Viral replication was needed for RV-induced mucin expression, and this induction was also dependent on TLR3, suggesting the involvement of double-stranded (ds) RNA signaling. Indeed, dsRNA alone could also induce mucin expres...

E J Ter Borg - One of the best experts on this subject based on the ideXlab platform.

  • measurement of increases in anti double stranded dna antibody levels as a predictor of Disease Exacerbation in systemic lupus erythematosus a long term prospective study
    Arthritis & Rheumatism, 1990
    Co-Authors: E J Ter Borg, G Horst, E J Hummel, P C Limburg, C G M Kallenberg
    Abstract:

    To evaluate the predictive power of changes in levels of antibodies to double-stranded DNA (anti-dsDNA) as a predictor of Disease Exacerbations in systemic lupus erythematosus (SLE), we performed a prospective study on 72 unselected patients with SLE (mean duration of study 18.5 months, range 6–35 months). Patients were seen at least once every 3 months, and Disease activity was scored according to a specific protocol. Plasma samples were obtained at least once every month and were assessed for anti-dsDNA antibody (by the Crithidia luciliae assay, an enzymelinked immunosorbent assay [ELISA], and the Farr assay) and for complement components C3 and C4. Twenty-seven of 33 Disease Exacerbations observed during the study period were accompanied by a positive test result for anti-dsDNA antibody (27 by the Farr assay, 19 by the C luciliae assay, and 23 by the ELISA). Twenty-four of these Exacerbations were preceded by a significant increase in anti-dsDNA antibody levels (23 by the Farr assay, 12 by the C luciliae assay, and 17 by the ELISA). The first observance of a significant increase in anti-dsDNA antibody levels preceded the Exacerbation by 8–10 weeks. Significant increases in anti-dsDNA antibody levels not followed by an Exacerbation were observed in 5 cases by the Farr assay, in 7 cases by the C luciliae assay, and in 3 cases by the ELISA; however, in 3 cases, 2 cases, and 1 case, respectively, these increases were followed by an increase in Disease activity that did not fulfill the criteria for an Exacerbation. Serial measurement of anti-dsDNA antibody levels was more sensitive for predicting Exacerbations than was measurement of C3 and/or C4 levels (P < 0.03). Serial assessment of anti-dsDNA antibody levels, especially by the Farr assay, is a sensitive and reasonably specific method for predicting Disease Exacerbations in SLE.

  • measurement of increases in anti double stranded dna antibody levels as a predictor of Disease Exacerbation in systemic lupus erythematosus a long term prospective study
    Arthritis & Rheumatism, 1990
    Co-Authors: E J Ter Borg, G Horst, E J Hummel, P C Limburg, C G M Kallenberg
    Abstract:

    : To evaluate the predictive power of changes in levels of antibodies to double-stranded DNA (anti-dsDNA) as a predictor of Disease Exacerbations in systemic lupus erythematosus (SLE), we performed a prospective study on 72 unselected patients with SLE (mean duration of study 18.5 months, range 6-35 months). Patients were seen at least once every 3 months, and Disease activity was scored according to a specific protocol. Plasma samples were obtained at least once every month and were assessed for anti-dsDNA antibody (by the Crithidia luciliae assay, an enzyme-linked immunosorbent assay [ELISA], and the Farr assay) and for complement components C3 and C4. Twenty-seven of 33 Disease Exacerbations observed during the study period were accompanied by a positive test result for anti-dsDNA antibody (27 by the Farr assay, 19 by the C luciliae assay, and 23 by the ELISA). Twenty-four of these Exacerbations were preceded by a significant increase in anti-dsDNA antibody levels (23 by the Farr assay, 12 by the C luciliae assay, and 17 by the ELISA). The first observance of a significant increase in anti-dsDNA antibody levels preceded the Exacerbation by 8-10 weeks. Significant increases in anti-dsDNA antibody levels not followed by an Exacerbation were observed in 5 cases by the Farr assay, in 7 cases by the C luciliae assay, and in 3 cases by the ELISA; however, in 3 cases, 2 cases, and 1 case, respectively, these increases were followed by an increase in Disease activity that did not fulfill the criteria for an Exacerbation. Serial measurement of anti-dsDNA antibody levels was more sensitive for predicting Exacerbations than was measurement of C3 and/or C4 levels (P less than 0.03). Serial assessment of anti-dsDNA antibody levels, especially by the Farr assay, is a sensitive and reasonably specific method for predicting Disease Exacerbations in SLE.

Yuhua Zhao - One of the best experts on this subject based on the ideXlab platform.

  • rhinovirus induced major airway mucin production involves a novel tlr3 egfr dependent pathway
    American Journal of Respiratory Cell and Molecular Biology, 2009
    Co-Authors: Yuhua Zhao, Dongfang Yu, Yin Chen
    Abstract:

    Mucociliary clearance is a critical innate defense system responsible for clearing up invading pathogens including bacteria and virus. Although the right amount of mucus is good, excessive mucus causes airway obstruction and tends to precipitate Disease symptoms. Rhinovirus (RV) is a common cold virus that causes asthma and chronic obstructive pulmonary Disease Exacerbation. Mucus overproduction has been linked to the pathogenesis of RV-induced Diseases and Disease Exacerbations. However, the molecular mechanism is not clear. In this study, using one of the major airway mucin-MUC5AC as marker, we found that both major and minor groups of RV induced mucin production in primary human epithelial cells and cell line. RV1A (a minor group of RV) could induce mucous cell metaplasia in vivo. Viral replication was needed for RV-induced mucin expression, and this induction was also dependent on TLR3, suggesting the involvement of double-stranded (ds) RNA signaling. Indeed, dsRNA alone could also induce mucin expres...

  • Rhinovirus-Induced Major Airway Mucin Production Involves a Novel TLR3-EGFR–Dependent Pathway
    American Journal of Respiratory Cell and Molecular Biology, 2008
    Co-Authors: Yuhua Zhao, Dongfang Yu, Yin Chen
    Abstract:

    Mucociliary clearance is a critical innate defense system responsible for clearing up invading pathogens including bacteria and virus. Although the right amount of mucus is good, excessive mucus causes airway obstruction and tends to precipitate Disease symptoms. Rhinovirus (RV) is a common cold virus that causes asthma and chronic obstructive pulmonary Disease Exacerbation. Mucus overproduction has been linked to the pathogenesis of RV-induced Diseases and Disease Exacerbations. However, the molecular mechanism is not clear. In this study, using one of the major airway mucin-MUC5AC as marker, we found that both major and minor groups of RV induced mucin production in primary human epithelial cells and cell line. RV1A (a minor group of RV) could induce mucous cell metaplasia in vivo. Viral replication was needed for RV-induced mucin expression, and this induction was also dependent on TLR3, suggesting the involvement of double-stranded (ds) RNA signaling. Indeed, dsRNA alone could also induce mucin expres...

Diane Mcmahonpratt - One of the best experts on this subject based on the ideXlab platform.

  • murine visceral leishmaniasis igm and polyclonal b cell activation lead to Disease Exacerbation
    European Journal of Immunology, 2010
    Co-Authors: Eszter Deak, Asha Jayakumar, Karen Goldsmithpestana, Blaise Dondji, John D Lambris, Diane Mcmahonpratt
    Abstract:

    In visceral leishmaniasis, the draining LN (DLN) is the initial site for colonization and establishment of infection after intradermal transmission by the sand fly vector; however, little is known about the developing immune response within this site. Using an intradermal infection model, which allows for parasite visceralization, we have examined the ongoing immune responses in the DLN of BALB/c mice infected with Leishmania infantum. Although not unexpected, at early times post-infection there is a marked B-cell expansion in the DLN, which persists throughout infection. However, the characteristics of this response were of interest; as early as day 7 post-infection, polyclonal antibodies (TNP, OVA, chromatin) were observed and the levels appeared comparable to the specific anti-leishmania response. Although B-cell-deficient JhD BALB/c mice are relatively resistant to infection, neither B-cell-derived IL-10 nor B-cell antigen presentation appear to be primarily responsible for the elevated parasitemia. However, passive transfer and reconstitution of JhD BALB/c with secretory immunoglobulins, (IgM or IgG; specific or non-specific immune complexes) results in increased susceptibility to L. infantum infection. Further, JhD BALB/c mice transgenetically reconstituted to secrete IgM demonstrated exacerbated Disease in comparison to WT BALB/c mice as early as 2 days post-infection. Evidence suggests that complement activation (generation of C5a) and signaling via the C5a receptor (CD88) is related to the Disease Exacerbation caused by IgM rather than cytokine levels (IL-10 or IFN-γ). Overall these studies indicate that polyclonal B-cell activation, which is known to be associated with human visceral leishmaniasis, is an early and intrinsic characteristic of Disease and may represent a target for therapeutic intervention.