The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Ruo Yuan - One of the best experts on this subject based on the ideXlab platform.
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highly efficient luminol immobilization approach and exponential strand Displacement Reaction based electrochemiluminescent strategy for monitoring microrna expression in cell
Biosensors and Bioelectronics, 2019Co-Authors: Yali Yuan, Haijun Wang, Yaqin Chai, Anyi Chen, Ruo YuanAbstract:Abstract This work used 3,4,9,10-perylenetetracarboxylic acid-luminol composite (PTCA-luminol) as signal tag with improved ECL signal and applied cruciform DNA structure mediated exponential strand Displacement Reaction (SDR) to construct an ultrasensitive electrochemiluminescence (ECL) biosensor for microRNA-21 (miRNA-21) detection. The novel luminol-based signal tags was synthesized utilizing the π-π stacking interaction between PTCA and luminol, realizing highly effective and stable immobilization of luminol and resulting in good stability and strong ECL response. Meanwhile, target miRNA-21 triggered disaggregation of cruciform DNA structure was used to mediate exponential SDR for target recycling amplification. Taking advantage of the novel luminol-based signal tag and exponential SDR, the proposed ECL biosensor achieved excellent sensitivity with wide linear range from 10 aM to 100 pM and detection limit was 2 aM. Moreover, this ECL biosensor was applied to estimate the expression level of miRNA-21 and pharmacodynamics of matrine in human breast cancer cells (MCF-7 cells). The proposed biosensor provided a new opportunity for the preparation of ECL nanomaterials and exhibited great application potential in other biomarkers detection, clinical application and pharmacodynamics evaluation.
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Reversible and Distance-Controllable DNA Scissor: A Regenerated Electrochemiluminescence Biosensing Platform for Ultrasensitive Detection of MicroRNA
2019Co-Authors: Lichun Peng, Yaqin Chai, Yali Yuan, Pu Zhang, Xianxue Gan, Ruo YuanAbstract:Reversing the switching of DNA scissors with precisely control remains a compelling goal. Herein, based on strand Displacement Reaction within single step, the DNA scissor realized reversible switching and further controlled the distance of end strands along the movement of DNA scissor, which has been applied for the development of a regenerated sensing platform for the ultrasensitive detection of microRNA-21 (miRNA-21) with the electrochemiluminescence (ECL) complex (PEI-Ru(II)) as luminophores and diethylenetriamine (DETA) as the coreactant. In the presence of ferrocene-labeled DNA (Fc-DNA), the DETA-labeled DNA scissor clockwise switched to “off” state based on strand Displacement Reaction, resulting in the significant ECL quenching of Ru(II) system. Next, by using miRNA-21 as the motive fuel, the configuration of DNA scissor could be anticlockwise switched, which significantly enhanced the ECL intensity of Ru(II) complex due to the releasing of Fc-DNA and the proximity between DETA and Ru(II) complex. The reversible switching of DNA scissor led to the remarkably enhancing of ECL signal, realizing ultrasensitive detection of miRNA-21 with an excellent detection limit of 0.17 fM, which was also applied in miRNA detection successfully from different cancer cells. Impressively, the reversible switching of DNA scissor biosensor was able to realize the regeneration of the biosensing platform by adding an additional single stranded DNA (ssDNA) based on strand Displacement Reaction within a single step, providing a novel concept for constructing simple and sensitive regenerated biosensor
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application of antibody powered triplex dna nanomachine to electrochemiluminescence biosensor for the detection of anti digoxigenin with improved sensitivity versus cycling strand Displacement Reaction
ACS Applied Materials & Interfaces, 2018Co-Authors: Shanshan Yang, Yaqin Chai, Ruo Yuan, Minghui Jiang, Ying ZhuoAbstract:The accurate and rapid quantitative detection of antibodies had a significant influence in controlling and preventing disease or toxin outbreaks. In this work, we first introduce the antibody-powered triplex-DNA nanomachine to release cargo DNA as a substitute target for sensitive electrochemiluminescence (ECL) detection of anti-digoxigenin based on a novel ternary ECL system. It is worth noting that the cargo DNA as a substitute target of antibody can further participate in an enzyme-assisted cycling strand Displacement Reaction to achieve ECL signal amplification and improve the sensitivity of antibody detection. Additionally, porous palladium nanospheres with a considerable catalytic activity were first applied as a coReaction accelerator to efficiently enhance the intensity of the ECL system of rubrene microblocks as luminophore and dissolved O2 as an endogenous coreactant. With the resultant ternary ECL system as a biosensing platform, a significantly enhanced initial signal was achieved in advance. ...
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ultrasensitive fluorescent assay based on a rolling circle amplification assisted multisite strand Displacement Reaction signal amplification strategy
Analytical Chemistry, 2018Co-Authors: Xin Peng, Yaqin Chai, Wenbin Liang, Zhibin Wen, Chengyi Xiong, Yingning Zheng, Ruo YuanAbstract:Heavy metal ions are persistent environmental contaminants and pose a great threat to human health, which has prompted demand for new methods to selectively identify and detect these metal ions. Herein, a novel fluorescent assay based on a rolling-circle-amplification (RCA)-assisted multisite-strand-Displacement-Reaction (SDR) signal-amplification strategy was proposed for the ultrasensitive detection of heavy metal ions with lead ions (Pb2+) as a model. The proposed strategy not only achieved the target recycling but also introduced RCA induced by released DNAzyme. Most importantly, the RCA product was adapted as the initiator to provide multiple sites for SDR, which could displace signal duplexes from RCA products to effectively avoid the self-quenching of signal-probe assembly on the RCA product. Therefore, the amplification efficiency and the detection sensitivity could be improved significantly. As expected, the proposed strategy demonstrated good performance for the determination of Pb2+ with a line...
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Ultrasensitive Fluorescent Assay Based on a Rolling-Circle-Amplification-Assisted Multisite-Strand-Displacement-Reaction Signal-Amplification Strategy
2018Co-Authors: Xin Peng, Yaqin Chai, Wenbin Liang, Zhibin Wen, Chengyi Xiong, Yingning Zheng, Ruo YuanAbstract:Heavy metal ions are persistent environmental contaminants and pose a great threat to human health, which has prompted demand for new methods to selectively identify and detect these metal ions. Herein, a novel fluorescent assay based on a rolling-circle-amplification (RCA)-assisted multisite-strand-Displacement-Reaction (SDR) signal-amplification strategy was proposed for the ultrasensitive detection of heavy metal ions with lead ions (Pb2+) as a model. The proposed strategy not only achieved the target recycling but also introduced RCA induced by released DNAzyme. Most importantly, the RCA product was adapted as the initiator to provide multiple sites for SDR, which could displace signal duplexes from RCA products to effectively avoid the self-quenching of signal-probe assembly on the RCA product. Therefore, the amplification efficiency and the detection sensitivity could be improved significantly. As expected, the proposed strategy demonstrated good performance for the determination of Pb2+ with a linear range from 0.1 to 50 nM and a detection limit down to 0.03 nM. Using this strategy for intracellular-Pb2+ detection, a favorable property was obtained. Furthermore, the proposed strategy could be also expanded for the determination of microRNA, proteins, and other biomolecules, offering a novel avenue for environmental assays and clinical diagnostics
Yaqin Chai - One of the best experts on this subject based on the ideXlab platform.
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highly efficient luminol immobilization approach and exponential strand Displacement Reaction based electrochemiluminescent strategy for monitoring microrna expression in cell
Biosensors and Bioelectronics, 2019Co-Authors: Yali Yuan, Haijun Wang, Yaqin Chai, Anyi Chen, Ruo YuanAbstract:Abstract This work used 3,4,9,10-perylenetetracarboxylic acid-luminol composite (PTCA-luminol) as signal tag with improved ECL signal and applied cruciform DNA structure mediated exponential strand Displacement Reaction (SDR) to construct an ultrasensitive electrochemiluminescence (ECL) biosensor for microRNA-21 (miRNA-21) detection. The novel luminol-based signal tags was synthesized utilizing the π-π stacking interaction between PTCA and luminol, realizing highly effective and stable immobilization of luminol and resulting in good stability and strong ECL response. Meanwhile, target miRNA-21 triggered disaggregation of cruciform DNA structure was used to mediate exponential SDR for target recycling amplification. Taking advantage of the novel luminol-based signal tag and exponential SDR, the proposed ECL biosensor achieved excellent sensitivity with wide linear range from 10 aM to 100 pM and detection limit was 2 aM. Moreover, this ECL biosensor was applied to estimate the expression level of miRNA-21 and pharmacodynamics of matrine in human breast cancer cells (MCF-7 cells). The proposed biosensor provided a new opportunity for the preparation of ECL nanomaterials and exhibited great application potential in other biomarkers detection, clinical application and pharmacodynamics evaluation.
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Reversible and Distance-Controllable DNA Scissor: A Regenerated Electrochemiluminescence Biosensing Platform for Ultrasensitive Detection of MicroRNA
2019Co-Authors: Lichun Peng, Yaqin Chai, Yali Yuan, Pu Zhang, Xianxue Gan, Ruo YuanAbstract:Reversing the switching of DNA scissors with precisely control remains a compelling goal. Herein, based on strand Displacement Reaction within single step, the DNA scissor realized reversible switching and further controlled the distance of end strands along the movement of DNA scissor, which has been applied for the development of a regenerated sensing platform for the ultrasensitive detection of microRNA-21 (miRNA-21) with the electrochemiluminescence (ECL) complex (PEI-Ru(II)) as luminophores and diethylenetriamine (DETA) as the coreactant. In the presence of ferrocene-labeled DNA (Fc-DNA), the DETA-labeled DNA scissor clockwise switched to “off” state based on strand Displacement Reaction, resulting in the significant ECL quenching of Ru(II) system. Next, by using miRNA-21 as the motive fuel, the configuration of DNA scissor could be anticlockwise switched, which significantly enhanced the ECL intensity of Ru(II) complex due to the releasing of Fc-DNA and the proximity between DETA and Ru(II) complex. The reversible switching of DNA scissor led to the remarkably enhancing of ECL signal, realizing ultrasensitive detection of miRNA-21 with an excellent detection limit of 0.17 fM, which was also applied in miRNA detection successfully from different cancer cells. Impressively, the reversible switching of DNA scissor biosensor was able to realize the regeneration of the biosensing platform by adding an additional single stranded DNA (ssDNA) based on strand Displacement Reaction within a single step, providing a novel concept for constructing simple and sensitive regenerated biosensor
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application of antibody powered triplex dna nanomachine to electrochemiluminescence biosensor for the detection of anti digoxigenin with improved sensitivity versus cycling strand Displacement Reaction
ACS Applied Materials & Interfaces, 2018Co-Authors: Shanshan Yang, Yaqin Chai, Ruo Yuan, Minghui Jiang, Ying ZhuoAbstract:The accurate and rapid quantitative detection of antibodies had a significant influence in controlling and preventing disease or toxin outbreaks. In this work, we first introduce the antibody-powered triplex-DNA nanomachine to release cargo DNA as a substitute target for sensitive electrochemiluminescence (ECL) detection of anti-digoxigenin based on a novel ternary ECL system. It is worth noting that the cargo DNA as a substitute target of antibody can further participate in an enzyme-assisted cycling strand Displacement Reaction to achieve ECL signal amplification and improve the sensitivity of antibody detection. Additionally, porous palladium nanospheres with a considerable catalytic activity were first applied as a coReaction accelerator to efficiently enhance the intensity of the ECL system of rubrene microblocks as luminophore and dissolved O2 as an endogenous coreactant. With the resultant ternary ECL system as a biosensing platform, a significantly enhanced initial signal was achieved in advance. ...
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ultrasensitive fluorescent assay based on a rolling circle amplification assisted multisite strand Displacement Reaction signal amplification strategy
Analytical Chemistry, 2018Co-Authors: Xin Peng, Yaqin Chai, Wenbin Liang, Zhibin Wen, Chengyi Xiong, Yingning Zheng, Ruo YuanAbstract:Heavy metal ions are persistent environmental contaminants and pose a great threat to human health, which has prompted demand for new methods to selectively identify and detect these metal ions. Herein, a novel fluorescent assay based on a rolling-circle-amplification (RCA)-assisted multisite-strand-Displacement-Reaction (SDR) signal-amplification strategy was proposed for the ultrasensitive detection of heavy metal ions with lead ions (Pb2+) as a model. The proposed strategy not only achieved the target recycling but also introduced RCA induced by released DNAzyme. Most importantly, the RCA product was adapted as the initiator to provide multiple sites for SDR, which could displace signal duplexes from RCA products to effectively avoid the self-quenching of signal-probe assembly on the RCA product. Therefore, the amplification efficiency and the detection sensitivity could be improved significantly. As expected, the proposed strategy demonstrated good performance for the determination of Pb2+ with a line...
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Ultrasensitive Fluorescent Assay Based on a Rolling-Circle-Amplification-Assisted Multisite-Strand-Displacement-Reaction Signal-Amplification Strategy
2018Co-Authors: Xin Peng, Yaqin Chai, Wenbin Liang, Zhibin Wen, Chengyi Xiong, Yingning Zheng, Ruo YuanAbstract:Heavy metal ions are persistent environmental contaminants and pose a great threat to human health, which has prompted demand for new methods to selectively identify and detect these metal ions. Herein, a novel fluorescent assay based on a rolling-circle-amplification (RCA)-assisted multisite-strand-Displacement-Reaction (SDR) signal-amplification strategy was proposed for the ultrasensitive detection of heavy metal ions with lead ions (Pb2+) as a model. The proposed strategy not only achieved the target recycling but also introduced RCA induced by released DNAzyme. Most importantly, the RCA product was adapted as the initiator to provide multiple sites for SDR, which could displace signal duplexes from RCA products to effectively avoid the self-quenching of signal-probe assembly on the RCA product. Therefore, the amplification efficiency and the detection sensitivity could be improved significantly. As expected, the proposed strategy demonstrated good performance for the determination of Pb2+ with a linear range from 0.1 to 50 nM and a detection limit down to 0.03 nM. Using this strategy for intracellular-Pb2+ detection, a favorable property was obtained. Furthermore, the proposed strategy could be also expanded for the determination of microRNA, proteins, and other biomolecules, offering a novel avenue for environmental assays and clinical diagnostics
Liang Tang - One of the best experts on this subject based on the ideXlab platform.
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single primer based multisite strand Displacement Reaction amplification strategy for rapid detection of terminal deoxynucleotidyl transferase activity
Analytical Chemistry, 2019Co-Authors: Xinyan Liu, Hao Wang, Keqin Deng, Sharon Kwee, Haowen Huang, Liang TangAbstract:A fluorescence-based multisite strand Displacement Reaction (MSSDR) amplification strategy is developed for the rapid, sensitive, and selective detection the activity of terminal deoxynucleotidyl t...
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Single Primer Based Multisite Strand Displacement Reaction Amplification Strategy for Rapid Detection of Terminal Deoxynucleotidyl Transferase Activity
2019Co-Authors: Xinyan Liu, Hao Wang, Keqin Deng, Sharon Kwee, Haowen Huang, Liang TangAbstract:A fluorescence-based multisite strand Displacement Reaction (MSSDR) amplification strategy is developed for the rapid, sensitive, and selective detection the activity of terminal deoxynucleotidyl transferase (TdT). Oligo dT primer was used for the TdT extension Reaction, then the left oligo dT primers were hybridized to the TdT extension Reaction product by end to end tiled style and initiated the MSSDR by Klenow polymerase, subsequently, 3′ terminals of these single-strand DNA produced by MSSDR are folded back to complement themselves with the adjacent sequences, and Klenow polymerase makes it into double-stranded DNA (dsDNA). The final dsDNA products were analyzed via dsDNA specific fluorescent dye. This method enables rapid (less than 100 min) and sensitive (limit of detection, LOD, 1.35 × 10–5 U) detection and has been demonstrated to work well using a real biosample. Our design would not only serve as a new prototype for high-throughput automated analysis and clinic diagnostic application but also has promising potential for improving the sensitivity of those TDT related biosensing system
Keqin Deng - One of the best experts on this subject based on the ideXlab platform.
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single primer based multisite strand Displacement Reaction amplification strategy for rapid detection of terminal deoxynucleotidyl transferase activity
Analytical Chemistry, 2019Co-Authors: Xinyan Liu, Hao Wang, Keqin Deng, Sharon Kwee, Haowen Huang, Liang TangAbstract:A fluorescence-based multisite strand Displacement Reaction (MSSDR) amplification strategy is developed for the rapid, sensitive, and selective detection the activity of terminal deoxynucleotidyl t...
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Single Primer Based Multisite Strand Displacement Reaction Amplification Strategy for Rapid Detection of Terminal Deoxynucleotidyl Transferase Activity
2019Co-Authors: Xinyan Liu, Hao Wang, Keqin Deng, Sharon Kwee, Haowen Huang, Liang TangAbstract:A fluorescence-based multisite strand Displacement Reaction (MSSDR) amplification strategy is developed for the rapid, sensitive, and selective detection the activity of terminal deoxynucleotidyl transferase (TdT). Oligo dT primer was used for the TdT extension Reaction, then the left oligo dT primers were hybridized to the TdT extension Reaction product by end to end tiled style and initiated the MSSDR by Klenow polymerase, subsequently, 3′ terminals of these single-strand DNA produced by MSSDR are folded back to complement themselves with the adjacent sequences, and Klenow polymerase makes it into double-stranded DNA (dsDNA). The final dsDNA products were analyzed via dsDNA specific fluorescent dye. This method enables rapid (less than 100 min) and sensitive (limit of detection, LOD, 1.35 × 10–5 U) detection and has been demonstrated to work well using a real biosample. Our design would not only serve as a new prototype for high-throughput automated analysis and clinic diagnostic application but also has promising potential for improving the sensitivity of those TDT related biosensing system
Wei Jiang - One of the best experts on this subject based on the ideXlab platform.
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toehold mediated strand Displacement Reaction dependent fluorescent strategy for sensitive detection of uracil dna glycosylase activity
Biosensors and Bioelectronics, 2017Co-Authors: Lei Wang, Wei JiangAbstract:Sensitive detection of uracil-DNA glycosylase (UDG) activity is beneficial for evaluating the repairing process of DNA lesions. Here, toehold-mediated strand Displacement Reaction (TSDR)-dependent fluorescent strategy was constructed for sensitive detection of UDG activity. A single-stranded DNA (ssDNA) probe with two uracil bases and a trigger sequence were designed. A hairpin probe with toehold domain was designed, and a reporter probe was also designed. Under the action of UDG, two uracil bases were removed from ssDNA probe, generating apurinic/apyrimidinic (AP) sites. Then, the AP sites could inhibit the TSDR between ssDNA probe and hairpin probe, leaving the trigger sequence in ssDNA probe still free. Subsequently, the trigger sequence was annealed with the reporter probe, initiating the polymerization and nicking amplification Reaction. As a result, numerous G-quadruplex (G4) structures were formed, which could bind with N-methyl-mesoporphyrin IX (NMM) to generate enhanced fluorescent signal. In the absence of UDG, the ssDNA probe could hybridize with the toehold domain of the hairpin probe to initiate TSDR, blocking the trigger sequence, and then the subsequent amplification Reaction would not occur. The proposed strategy was successfully implemented for detecting UDG activity with a detection limit of 2.7×10-5U/mL. Moreover, the strategy could distinguish UDG well from other interference enzymes. Furthermore, the strategy was also applied for detecting UDG activity in HeLa cells lysate with low effect of cellular components. These results indicated that the proposed strategy offered a promising tool for sensitive quantification of UDG activity in UDG-related function study and disease prognosis.
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highly selective and sensitive detection of mirna based on toehold mediated strand Displacement Reaction and dna tetrahedron substrate
Biosensors and Bioelectronics, 2015Co-Authors: Wei Jiang, Yongshun Ding, Lei WangAbstract:MicroRNAs (miRNAs) play important roles in a variety of biological processes and have been regarded as tumor biomarkers in cancer diagnosis and prognosis. In this work, a single-molecule counting method for miRNA analysis was proposed based on toehold-mediated strand Displacement Reaction (SDR) and DNA tetrahedron substrate. Firstly, a specially designed DNA tetrahedron was assembled with a hairpin at one of the vertex, which has an overhanging toehold domain. Then, the DNA tetrahedron was immobilized on the epoxy-functional glass slide by epoxy-amine Reaction, forming a DNA tetrahedron substrate. Next, the target miRNA perhybridized with the toehold domain and initiated a strand Displacement Reaction along with the unfolding of the hairpin, realizing the selective recognization of miRNA. Finally, a biotin labeled detection DNA was hybridized with the new emerging single strand and the streptavidin coated QDs were used as fluorescent probes. Fluorescent images were acquired via epi-fluorescence microscopy, the numbers of fluorescence dots were counted one by one for quantification. The detection limit is 5 fM, which displayed an excellent sensitivity. Moreover, the proposed method which can accurately be identified the target miRNA among its family members, demonstrated an admirable selectivity. Furthermore, miRNA analysis in total RNA samples from human lung tissues was performed, suggesting the feasibility of this method for quantitative detection of miRNA in biomedical research and early clinical diagnostics.
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toehold mediated strand Displacement Reaction triggered isothermal dna amplification for highly sensitive and selective fluorescent detection of single base mutation
Biosensors and Bioelectronics, 2014Co-Authors: Jing Zhu, Lei Wang, Yongshun Ding, Xingti Liu, Wei JiangAbstract:Highly sensitive and selective detection strategy for single-base mutations is essential for risk assessment of malignancy and disease prognosis. In this work, a fluorescent detection method for single-base mutation was proposed based on high selectivity of toehold-mediated strand Displacement Reaction (TSDR) and powerful signal amplification capability of isothermal DNA amplification. A discrimination probe was specially designed with a stem-loop structure and an overhanging toehold domain. Hybridization between the toehold domain and the perfect matched target initiated the TSDR along with the unfolding of the discrimination probe. Subsequently, the target sequence acted as a primer to initiate the polymerization and nicking Reactions, which released a great abundant of short sequences. Finally, the released strands were annealed with the reporter probe, launching another polymerization and nicking Reaction to produce lots of G-quadruplex DNA, which could bind the N-methyl mesoporphyrin IX to yield an enhanced fluorescence response. However, when there was even a single base mismatch in the target DNA, the TSDR was suppressed and so subsequent isothermal DNA amplification and fluorescence response process could not occur. The proposed approach has been successfully implemented for the identification of the single-base mutant sequences in the human KRAS gene with a detection limit of 1.8 pM. Furthermore, a recovery of 90% was obtained when detecting the target sequence in spiked HeLa cells lysate, demonstrating the feasibility of this detection strategy for single-base mutations in biological samples.