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Gerald W. Feigenson - One of the best experts on this subject based on the ideXlab platform.
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phase diagram of a 4 component lipid mixture dspc dopc popc chol
Biochimica et Biophysica Acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.
Biochimica et biophysica acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
Tatyana M. Konyakhina - One of the best experts on this subject based on the ideXlab platform.
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phase diagram of a 4 component lipid mixture dspc dopc popc chol
Biochimica et Biophysica Acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.
Biochimica et biophysica acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
James D. Mastroianni - One of the best experts on this subject based on the ideXlab platform.
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phase diagram of a 4 component lipid mixture dspc dopc popc chol
Biochimica et Biophysica Acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.
Biochimica et biophysica acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
Frederick A. Heberle - One of the best experts on this subject based on the ideXlab platform.
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phase diagram of a 4 component lipid mixture dspc dopc popc chol
Biochimica et Biophysica Acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.
Biochimica et biophysica acta, 2013Co-Authors: Tatyana M. Konyakhina, Frederick A. Heberle, James D. Mastroianni, Gerald W. FeigensonAbstract:Abstract We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (Distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld + Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld + Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.
Katarina Edwards - One of the best experts on this subject based on the ideXlab platform.
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polyethylene glycol stabilized lipid disks as model membranes in interaction studies based on electrokinetic capillary chromatography and quartz crystal microbalance
Analytical Biochemistry, 2011Co-Authors: Kati Vainikka, Katarina Edwards, Karin Reijmar, Gebrenegus Yohannes, Jorgen Samuelsson, Matti Jussila, Marjaliisa RiekkolaAbstract:Distearoylphosphatidylcholine (DSPC)/cholesterol/distearoylphosphatidylethanolamine (DSPE)-polyethylene glycol 5000 [PEG(5000)] lipid disks, mimicking biological membranes, were used as pseudostationary phase in partial filling electrokinetic capillary chromatography (EKC) to study interactions between pharmaceuticals and lipid disks. Capillaries were coated either noncovalently with a poly(1-vinylpyrrolidone)-based copolymer or covalently with polyacrylamide to mask the negative charges of the fused-silica capillary wall and to minimize interactions between positively charged pharmaceuticals and capillary wall. Although the noncovalent copolymer coating method was faster, better stability of the covalent polyacrylamide coating at physiological pH 7.4 made it more reliable in partial filling EKC studies. Migration times of pharmaceuticals were proportional to the amount of lipids in the pseudostationary phase, and partition coefficients were successfully determined. Because the capillary coatings almost totally suppressed the electroosmotic flow, it was not practical to use the EKC-based method for partition studies involving large molecules with low mobilities. Hence, the applicability of the biomembrane mimicking lipid disks for interactions studies with large molecules was verified by the quartz crystal microbalance technique. Biotinylated lipid disks were then immobilized on streptavidin-coated sensor chip surface, and interactions with a high-molecular-mass molecule, lysozyme, were studied. Cryo-transmission electron microscopy and asymmetrical flow field-flow fractionation were used to clarify the sizes of lipid disks used.
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Liposomes, Disks, and Spherical Micelles: Aggregate Structure in Mixtures of Gel Phase Phosphatidylcholines and Poly(Ethylene Glycol)-Phospholipids
Biophysical journal, 2003Co-Authors: Markus Johnsson, Katarina EdwardsAbstract:Poly(ethylene glycol) (PEG) decorated lipid bilayers are widely used in biomembrane and pharmaceutical research. The success of PEG-lipid stabilized liposomes in drug delivery is one of the key factors for the interest in these polymer/lipid systems. From a more fundamental point of view, it is essential to understand the effect of the surface grafted polymers on the physical-chemical properties of the lipid bilayer. Herein we have used cryo-transmission electron microscopy and dynamic light scattering to characterize the aggregate structure and phase behavior of mixtures of PEG-lipids and Distearoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The PEG-lipids contain PEG of molecular weight 2000 or 5000. We show that the transition from a dispersed lamellar phase (liposomes) to a micellar phase consisting of small spherical micelles occurs via the formation of small discoidal micelles. The onset of disk formation already takes place at low PEG-lipid concentrations (