The Experts below are selected from a list of 129 Experts worldwide ranked by ideXlab platform
B L Dalkin - One of the best experts on this subject based on the ideXlab platform.
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Dithiothreitol effects on human sperm quality.
The Journal of Urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:AbstractHuman semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro.We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propi...
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Dithiothreitol effects on human sperm quality.
The Journal of urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:Human semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro. We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propidium iodide and the mitochondria specific fluorochrome rhodamine-123, respectively. Acrosomal integrity was determined using the fluorescent stain chlortetracycline. Sperm morphology was evaluated using bright field microscopy. For completely liquefied semen (35 cases) Dithiothreitol reduced sperm motility (59.1 +/- 1.2% untreated versus 53.2 +/- 1.2% treated, p < 0.01) and motion characteristics. However, Dithiothreitol had no statistically significant effect on motility on sperm in the group with incompletely liquefied semen (10 cases). Sperm cell membrane, mitochondrial and acrosomal integrity was unaffected by Dithiothreitol regardless of liquefaction status. Dithiothreitol caused a significant increase in abnormally large sperm head morphology in the group with completely liquefied semen. The minimal effects of Dithiothreitol on sperm motility traits and viability support its use as a possible aid in the evaluation and manipulation of incompletely liquefied semen.
M J Barmatz - One of the best experts on this subject based on the ideXlab platform.
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Dithiothreitol effects on human sperm quality.
The Journal of Urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:AbstractHuman semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro.We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propi...
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Dithiothreitol effects on human sperm quality.
The Journal of urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:Human semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro. We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propidium iodide and the mitochondria specific fluorochrome rhodamine-123, respectively. Acrosomal integrity was determined using the fluorescent stain chlortetracycline. Sperm morphology was evaluated using bright field microscopy. For completely liquefied semen (35 cases) Dithiothreitol reduced sperm motility (59.1 +/- 1.2% untreated versus 53.2 +/- 1.2% treated, p < 0.01) and motion characteristics. However, Dithiothreitol had no statistically significant effect on motility on sperm in the group with incompletely liquefied semen (10 cases). Sperm cell membrane, mitochondrial and acrosomal integrity was unaffected by Dithiothreitol regardless of liquefaction status. Dithiothreitol caused a significant increase in abnormally large sperm head morphology in the group with completely liquefied semen. The minimal effects of Dithiothreitol on sperm motility traits and viability support its use as a possible aid in the evaluation and manipulation of incompletely liquefied semen.
D S Karabinus - One of the best experts on this subject based on the ideXlab platform.
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Dithiothreitol effects on human sperm quality.
The Journal of Urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:AbstractHuman semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro.We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propi...
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Dithiothreitol effects on human sperm quality.
The Journal of urology, 1994Co-Authors: M J Barmatz, D S Karabinus, B L DalkinAbstract:Human semen normally coagulates immediately after ejaculation and then undergoes liquefaction during the next 15 to 60 minutes. Incomplete seminal liquefaction can result in impaired sperm motility and make clinical evaluation and manipulation difficult. Dithiothreitol, a mucolytic agent that reduces the mucoprotein disulfide bonds in sputum, has been found to induce liquefaction of incompletely liquefied semen in vitro. We studied the effects of Dithiothreitol on sperm motility, viability, acrosomal integrity and morphology. A semen sample was provided by 45 healthy, young men at the University of Arizona. Of the specimens 10 (22%) demonstrated incomplete liquefaction. Sperm motility and motion characteristics of untreated (control) semen and semen treated with Dithiothreitol were objectively evaluated using computer assisted semen analysis. Sperm cell membrane integrity and mitochondrial integrity were measured by fluorescence microscopy using the deoxyribonucleic acid specific fluorochrome propidium iodide and the mitochondria specific fluorochrome rhodamine-123, respectively. Acrosomal integrity was determined using the fluorescent stain chlortetracycline. Sperm morphology was evaluated using bright field microscopy. For completely liquefied semen (35 cases) Dithiothreitol reduced sperm motility (59.1 +/- 1.2% untreated versus 53.2 +/- 1.2% treated, p < 0.01) and motion characteristics. However, Dithiothreitol had no statistically significant effect on motility on sperm in the group with incompletely liquefied semen (10 cases). Sperm cell membrane, mitochondrial and acrosomal integrity was unaffected by Dithiothreitol regardless of liquefaction status. Dithiothreitol caused a significant increase in abnormally large sperm head morphology in the group with completely liquefied semen. The minimal effects of Dithiothreitol on sperm motility traits and viability support its use as a possible aid in the evaluation and manipulation of incompletely liquefied semen.
Pernille Andersen - One of the best experts on this subject based on the ideXlab platform.
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thirty three day storage of Dithiothreitol treated red blood cells used to eliminate daratumumab interference in serological testing
Vox Sanguinis, 2018Co-Authors: Henriette Lorenzen, Nazia Lone Akhtar, Maria Nielsen, Lea Svendsen, Pernille AndersenAbstract:BACKGROUND AND OBJECTIVES Daratumumab binds CD38 on red blood cells causing interference with indirect antiglobulin tests. Dithiothreitol is used to eliminate interference allowing detection of alloantibodies. Haemolysis is observed during storage of Dithiothreitol-treated antibody identification panel cells. The objective of this study was to develop a modified method for Dithiothreitol treatment to reduce the haemolysis during 33 days of storage and still be able to eliminate daratumumab interference. MATERIALS AND METHODS Panel cells were treated with various volumes of 0·2 m Dithiothreitol supplied by various manufacturers. Haemolysis Index of Dithiothreitol-treated and untreated panel cells was measured and compared on days 1, 15 and 33. Antibody screening tests with Dithiothreitol-treated screening cells were performed on samples from 15 daratumumab-treated patients (dose 16 mg/kg) and 34 patients with known alloantibodies. Antibody identifications with Dithiothreitol-treated panel cells were performed on seven additional known alloantibodies. RESULTS Dithiothreitol treatment with a ratio of 30:25 (red blood cells:Dithiothreitol) showed the same degree of haemolysis as with untreated panel cells. Daratumumab interference was eliminated in all 15 samples from daratumumab-treated patients. Twenty-six of 34 alloantibodies were detected, and all seven additional alloantibodies were identified using the modified Dithiothreitol treatment. Eight alloantibodies within the Kell system were negative. No decrease in the reaction strength was observed during the 33-day storage period. CONCLUSION The modified Dithiothreitol method was able to reduce haemolysis during storage and to detect and identify alloantibodies in the presence of daratumumab.
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Thirty‐three‐day storage of Dithiothreitol‐treated red blood cells used to eliminate daratumumab interference in serological testing
Vox sanguinis, 2018Co-Authors: Henriette Lorenzen, Nazia Lone Akhtar, Maria Nielsen, Lea Svendsen, Pernille AndersenAbstract:BACKGROUND AND OBJECTIVES Daratumumab binds CD38 on red blood cells causing interference with indirect antiglobulin tests. Dithiothreitol is used to eliminate interference allowing detection of alloantibodies. Haemolysis is observed during storage of Dithiothreitol-treated antibody identification panel cells. The objective of this study was to develop a modified method for Dithiothreitol treatment to reduce the haemolysis during 33 days of storage and still be able to eliminate daratumumab interference. MATERIALS AND METHODS Panel cells were treated with various volumes of 0·2 m Dithiothreitol supplied by various manufacturers. Haemolysis Index of Dithiothreitol-treated and untreated panel cells was measured and compared on days 1, 15 and 33. Antibody screening tests with Dithiothreitol-treated screening cells were performed on samples from 15 daratumumab-treated patients (dose 16 mg/kg) and 34 patients with known alloantibodies. Antibody identifications with Dithiothreitol-treated panel cells were performed on seven additional known alloantibodies. RESULTS Dithiothreitol treatment with a ratio of 30:25 (red blood cells:Dithiothreitol) showed the same degree of haemolysis as with untreated panel cells. Daratumumab interference was eliminated in all 15 samples from daratumumab-treated patients. Twenty-six of 34 alloantibodies were detected, and all seven additional alloantibodies were identified using the modified Dithiothreitol treatment. Eight alloantibodies within the Kell system were negative. No decrease in the reaction strength was observed during the 33-day storage period. CONCLUSION The modified Dithiothreitol method was able to reduce haemolysis during storage and to detect and identify alloantibodies in the presence of daratumumab.
Guannan Wang - One of the best experts on this subject based on the ideXlab platform.
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High sensitive ratiometric fluorescence analysis of trypsin and Dithiothreitol based on WS2 QDs
Talanta, 2020Co-Authors: Xinhe Duan, Guannan WangAbstract:Abstract In this paper, a simple tungsten disulfide quantum dots (WS2 QDs)-based ratiometric fluorescence method was established for the detection of trypsin and 1, 4-Dithiothreitol. Trypsin can hydrolyze cytochrome c into heme-peptide fragments with peroxidase-like activity. In the presence of hydrogen peroxide, the fragments can generates hydroxyl radicals, which can oxidize o-phenylenediamine (OPD) to form 2,3-diaminophenazine (DAP) with a fluorescence peak at 568 nm. DAP can quench the fluorescence of WS2 QDs at 448 nm via fluorescence resonance energy transfer (FRET). When 1, 4-Dithiothreitol was present, it can react with hydroxyl radicals, and less OPD was oxidized, which accompanied by the fluorescence intensity of WS2 QDs increased and the fluorescence intensity of DAP decreased. Therefore, the fluorescence intensity ratio (F568/F448) can be used to monitor trypsin and 1, 4-Dithiothreitol. A good linear calibration between fluorescence intensity ratio F568/F448 versus trypsin activity and1, 4-Dithiothreitol concentration were obtained within 0.2–140 μg mL−1 and 20–200 μmol L−1, respectively. And the detection limit was 0.09 μg mL−1 for trypsin and 6.8 μmol L−1 for 1, 4-Dithiothreitol, respectively. Furthermore, the developed ratiometric fluorescence method was successfully applied for trypsin and 1, 4-Dithiothreitol assay in human serum samples.