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T L Pitt - One of the best experts on this subject based on the ideXlab platform.

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  • molecular characterization of brazilian human giardia duodenalis isolates using isoenzyme and random amplified polymorphic DNA Analysis
    Diagnostic Microbiology and Infectious Disease, 2003
    Co-Authors: Miriam Oliveira E Rocha, Maria Carmen Aires Gomes, Adriana Oliveira Costa, Cinthia Furst, Edward F Silva
    Abstract:

    Abstract Isoenzymes and RAPD (random amplified polymorphic DNA) Analysis were used to characterize three Brazilian human isolates of Giardia duodenalis and its clones. The Portland-1 strain (ATCC 30888) was included in the study as a reference pattern. Both methods divided the isolates into two main groups, one represented by the Portland-1 strain, the other constituted by the Brazilian isolates, which, in turn, were divided into 2 subgroups. The dendogram constructed with the RAPD data, using seven primers, revealed a great heterogeneity between Brazilian isolates and the Portland-1 strain. There was no relationship to the clinical characteristics of the isolates. Although a lot of similarity has been observed among Brazilian isolates and its clones, individual polymorphism was detected, which could be releated to the clonal reproduction of this protozoan.

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Satoshi Hori - One of the best experts on this subject based on the ideXlab platform.