The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
Yoshikazu Kurosawa - One of the best experts on this subject based on the ideXlab platform.
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natural occurrence of nuc in the sera of autoimmune prone mrl lpr mice
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
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Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
Yoshiyuki Kanai - One of the best experts on this subject based on the ideXlab platform.
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Induction of anti-DNA antibodies by immunization with anti-DNA antibodies: mechanism and characterization.
Lupus, 2000Co-Authors: F. Satake, Yoshiyuki Kanai, Naomi Watanabe, Nobuyuki Miyasaka, Tetsuo KubotaAbstract:Two well-characterized IgG monoclonal antibodies, reactive with double-stranded (ds) DNA and nucleosomes, were administered to normal BALB/c mice to examine the reproducibility and the biology of a previously reported model of anti-DNA Antibody induction by immunization with anti-DNA antibodies. The monoclonal antibodies were purified either with or without a high-salt wash to remove nucleosomal antigens bound to them during the cell culture. Both monoclonal antibodies, but not normal IgG, induced significant IgG anti-dsDNA Antibody production from 1 week to 25 weeks after the last immunization. The antibodies produced in this manner possess different binding preferences to ds synthetic polynucleotides than the antibodies used for the immunization, and they did not react with nucleosomes. The monoclonal antibodies purified with the high-salt wash were more effective in anti-DNA Antibody induction than those purified without the high-salt wash. Even when bound to these monoclonal antibodies, neither dsDNA, nucleosomes, or ds synthetic polynucleotides exert significant antigenicity. For example, anti-DNA antibodies produced by mice immunized with an immune complex formed by poly(dA-dT) and one of the monoclonal antibodies that has a high affinity to this polynucleotide did not show an increased affinity to poly(dA-dT). Together, these results suggest that anti-DNA Antibody molecules or processed Antibody peptides, and not DNA/nucleosomes carried by anti-DNA antibodies, play a role in this model of anti-DNA Antibody production.
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natural occurrence of nuc in the sera of autoimmune prone mrl lpr mice
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
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Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
Sei-ichi Tanuma - One of the best experts on this subject based on the ideXlab platform.
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natural occurrence of nuc in the sera of autoimmune prone mrl lpr mice
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
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Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
T. Uehara - One of the best experts on this subject based on the ideXlab platform.
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natural occurrence of nuc in the sera of autoimmune prone mrl lpr mice
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
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Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
Masayuki Amagai - One of the best experts on this subject based on the ideXlab platform.
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natural occurrence of nuc in the sera of autoimmune prone mrl lpr mice
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.
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Natural occurrence of Nuc in the sera of autoimmune-prone MRL/lpr mice.
Biochemical and Biophysical Research Communications, 1993Co-Authors: Yoshiyuki Kanai, Keiji Miura, Osamu Takeda, Sei-ichi Tanuma, T. Uehara, Masayuki Amagai, Yoshikazu KurosawaAbstract:Abstract We previously established a clone of cells termed KML1-7 which produces a soluble factor that boosts anti-DNA Antibody production both in vitro and in vivo across the H-2 barrier. By using the purified protein, termed nucleobindin (Nuc), we cloned cDNA and produced recombinant (r) Nuc in E.coli. Although the purified rNuc showed biological activities such as anti-DNA Antibody boosting and DNA binding, there was no evidence that Nuc is really associated with autoimmune status in lupus-prone MRL/lpr mice. Here we report that identification of Nuc was successful from the sera of MRL/lpr mice, but not from those of the substrain MRL/n mice, which show no apparent autoimmune syndrome at the same age of MRL/lpr mice, by means of immunochemical as well as N-terminal amino-acid sequencing methods.