The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Jiangwei Yan - One of the best experts on this subject based on the ideXlab platform.

  • genetic variability and phylogenetic analysis of 39 short tandem repeat loci in beijing han population
    Hereditas, 2015
    Co-Authors: Xiuyan Ruan, Weini Wang, Yaran Yang, Bingbing Xie, Jing Chen, Yacheng Liu, Jiangwei Yan
    Abstract:

    In this study, we studied the genetic polymorphisms of short tandem repeat (STR) loci from 13 CODIS and 26 non-CODIS system in Beijing Han population for the first time, and established a Database of 39 STR loci whose forensic parameters were further evaluated. Our results demonstrated no significant deviation from the Hardy-Weinberg equilibrium of 39 STR loci and no pairwise linkage disequilibrium between them. The power of discriminations, expected heterozygosity, polymorphic information content, and power of exclusion of 39 STR loci ranged from 0.7740-0.9818, 0.6000-0.9350, 0.5317-0.9047 and 0.2909-0.8673. The cumulated discrimination power and cumulative probability of exclusion were 0.999999999999999999999999999999999999999964971 and 0.999999999973878, respectively. Moreover, the genetic distance was calculated based on allele frequency and phylogenetic tree was built using STR loci data from Beijing Han and other 11 Chinese ethnic groups.This study provides important basic data for Chinese forensic DNA Database and population genetics Database, and has important significance in carrying out forensic individual identification, paternity testing, and population genetic study.

  • application of next generation sequencing technology in forensic science
    Genomics Proteomics & Bioinformatics, 2014
    Co-Authors: Yaran Yang, Bingbing Xie, Jiangwei Yan
    Abstract:

    Next-generation sequencing (NGS) technology, with its high-throughput capacity and low cost, has developed rapidly in recent years and become an important analytical tool for many genomics researchers. New opportunities in the research domain of the forensic studies emerge by harnessing the power of NGS technology, which can be applied to simultaneously analyzing multiple loci of forensic interest in different genetic contexts, such as autosomes, mitochondrial and sex chromosomes. Furthermore, NGS technology can also have potential applications in many other aspects of research. These include DNA Database construction, ancestry and phenotypic inference, monozygotic twin studies, body fluid and species identification, and forensic animal, plant and microbiological analyses. Here we review the application of NGS technology in the field of forensic science with the aim of providing a reference for future forensics studies and practice.

  • issues on china forensic DNA Database
    Journal of Forensic Medicine, 2011
    Co-Authors: Jiangwei Yan, Bruce Budowle, Ranajit Chakraborty, Arthur Eisenberg
    Abstract:

    This study investigates the effects of selection and number of selected autosomal STR loci for the forensic DNA Database applications,such as Database matching,familial searching,missing person identification,the effects of population substructure,and the Y chromosome and mitochondrial DNA as the supplemental tools to improve the searching results,and provides suggestions for further improving the China forensic DNA Database.

  • population genetic analysis of 15 str loci of chinese tu ethnic minority group
    Forensic Science International, 2008
    Co-Authors: Bofeng Zhu, Chunmei Shen, Jiangwei Yan, Yajun Deng, Xin Xiong, Yanqing Huang
    Abstract:

    We studied and established a DNA Database of 15 euchromosome STRs (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) in a population sample of 151 unrelated individuals of Tu ethnic minority group. Allelic frequencies and statistical parameters of Tu population were calculated. Totally 136 alleles were observed, with the corresponding allelic frequencies ranging from 0.0033 to 0.5359. Chi-square test showed that all STR loci agreed with Hardy-Weinberg equilibrium. Our study population data were compared with the previously publishing population data of other ethnic groups or areas. Our results of present study were valuable for human identification and paternity tests in Chinese Tu population.

  • population genetic polymorphisms for 17 y chromosomal strs haplotypes of chinese salar ethnic minority group
    Legal Medicine, 2007
    Co-Authors: Chunmei Shen, Jiangwei Yan, Bofeng Zhu, Xi Xun, Yajun Deng, Jun Zhu, Yao Liu
    Abstract:

    We studied and established a DNA Database of 17 Y-STRs in a population sample of 133 unrelated individuals of Salar ethnic minority group, in order to investigate haplotype frequencies of Salar population, evaluate their usefulness in forensic applications, and enrich Chinese population genetic informational resources. Out of a total of 133 individuals 123 showed different haplotypes, while six haplotypes occurred more than once. The overall haplotype diversity for the Y-STRs loci was 0.9983, and the discrimination capacity was 0.9248.

Norio Sugiura - One of the best experts on this subject based on the ideXlab platform.

  • detection and sequencing of the microcystin lr degrading gene mlra from new bacteria isolated from japanese lakes
    Fems Microbiology Letters, 2003
    Co-Authors: Takeshi Saito, Kunihiro Okano, Ho Dong Park, Tomoaki Itayama, Yuhei Inamori, Brett A Neilan, Brendan P Burns, Norio Sugiura
    Abstract:

    mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA Database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These strains possessed mlrA homologues, and the identities of the genes of MD-1 and Y2 with the corresponding MJ-PV exceeded 98% and 84%, respectively. On the other hand, the mlrA gene was not detected in laboratory strains of the closely related Sphingomonas spp. strains employing hemi-nested polymerase chain reaction detection using two primer sets. Although the microcystin-degrading bacteria were closely related strains, they did not cluster together as the same species. We can conclude that the mlrA gene is conserved in three different bacterial species, and it is unique to microcystin degraders but not to the genus Sphingomonas. : 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

  • detection and sequencing of the microcystin lr degrading gene mlra from new bacteria isolated from japanese lakes
    Fems Microbiology Letters, 2003
    Co-Authors: Takeshi Saito, Kunihiro Okano, Ho Dong Park, Tomoaki Itayama, Yuhei Inamori, Brett A Neilan, Brendan P Burns, Norio Sugiura
    Abstract:

    mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA Database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These strains possessed mlrA homologues, and the identities of the genes of MD-1 and Y2 with the corresponding MJ-PV exceeded 98% and 84%, respectively. On the other hand, the mlrA gene was not detected in laboratory strains of the closely related Sphingomonas spp. strains employing hemi-nested polymerase chain reaction detection using two primer sets. Although the microcystin-degrading bacteria were closely related strains, they did not cluster together as the same species. We can conclude that the mlrA gene is conserved in three different bacterial species, and it is unique to microcystin degraders but not to the genus Sphingomonas.

Pangchui Shaw - One of the best experts on this subject based on the ideXlab platform.

  • an integrated web medicinal materials DNA Database mmdbd medicinal materials DNA barcode Database
    BMC Genomics, 2010
    Co-Authors: Shaoke Lou, Kalok Wong, Paul Puihay But, Stephen Kwokwing Tsui, Pangchui Shaw
    Abstract:

    Thousands of plants and animals possess pharmacological properties and there is an increased interest in using these materials for therapy and health maintenance. Efficacies of the application is critically dependent on the use of genuine materials. For time to time, life-threatening poisoning is found because toxic adulterant or substitute is administered. DNA barcoding provides a definitive means of authentication and for conducting molecular systematics studies. Owing to the reduced cost in DNA authentication, the volume of the DNA barcodes produced for medicinal materials is on the rise and necessitates the development of an integrated DNA Database. We have developed an integrated DNA barcode multimedia information platform- Medicinal Materials DNA Barcode Database (MMDBD) for data retrieval and similarity search. MMDBD contains over 1000 species of medicinal materials listed in the Chinese Pharmacopoeia and American Herbal Pharmacopoeia. MMDBD also contains useful information of the medicinal material, including resources, adulterant information, medical parts, photographs, primers used for obtaining the barcodes and key references. MMDBD can be accessed at http://www.cuhk.edu.hk/icm/mmdbd.htm . This work provides a centralized medicinal materials DNA barcode Database and bioinformatics tools for data storage, analysis and exchange for promoting the identification of medicinal materials. MMDBD has the largest collection of DNA barcodes of medicinal materials and is a useful resource for researchers in conservation, systematic study, forensic and herbal industry.

Yajun Deng - One of the best experts on this subject based on the ideXlab platform.

  • population genetic analysis of 15 str loci of chinese tu ethnic minority group
    Forensic Science International, 2008
    Co-Authors: Bofeng Zhu, Chunmei Shen, Jiangwei Yan, Yajun Deng, Xin Xiong, Yanqing Huang
    Abstract:

    We studied and established a DNA Database of 15 euchromosome STRs (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA) in a population sample of 151 unrelated individuals of Tu ethnic minority group. Allelic frequencies and statistical parameters of Tu population were calculated. Totally 136 alleles were observed, with the corresponding allelic frequencies ranging from 0.0033 to 0.5359. Chi-square test showed that all STR loci agreed with Hardy-Weinberg equilibrium. Our study population data were compared with the previously publishing population data of other ethnic groups or areas. Our results of present study were valuable for human identification and paternity tests in Chinese Tu population.

  • population genetic polymorphisms for 17 y chromosomal strs haplotypes of chinese salar ethnic minority group
    Legal Medicine, 2007
    Co-Authors: Chunmei Shen, Jiangwei Yan, Bofeng Zhu, Xi Xun, Yajun Deng, Jun Zhu, Yao Liu
    Abstract:

    We studied and established a DNA Database of 17 Y-STRs in a population sample of 133 unrelated individuals of Salar ethnic minority group, in order to investigate haplotype frequencies of Salar population, evaluate their usefulness in forensic applications, and enrich Chinese population genetic informational resources. Out of a total of 133 individuals 123 showed different haplotypes, while six haplotypes occurred more than once. The overall haplotype diversity for the Y-STRs loci was 0.9983, and the discrimination capacity was 0.9248.

Takeshi Saito - One of the best experts on this subject based on the ideXlab platform.

  • detection and sequencing of the microcystin lr degrading gene mlra from new bacteria isolated from japanese lakes
    Fems Microbiology Letters, 2003
    Co-Authors: Takeshi Saito, Kunihiro Okano, Ho Dong Park, Tomoaki Itayama, Yuhei Inamori, Brett A Neilan, Brendan P Burns, Norio Sugiura
    Abstract:

    mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA Database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These strains possessed mlrA homologues, and the identities of the genes of MD-1 and Y2 with the corresponding MJ-PV exceeded 98% and 84%, respectively. On the other hand, the mlrA gene was not detected in laboratory strains of the closely related Sphingomonas spp. strains employing hemi-nested polymerase chain reaction detection using two primer sets. Although the microcystin-degrading bacteria were closely related strains, they did not cluster together as the same species. We can conclude that the mlrA gene is conserved in three different bacterial species, and it is unique to microcystin degraders but not to the genus Sphingomonas. : 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

  • detection and sequencing of the microcystin lr degrading gene mlra from new bacteria isolated from japanese lakes
    Fems Microbiology Letters, 2003
    Co-Authors: Takeshi Saito, Kunihiro Okano, Ho Dong Park, Tomoaki Itayama, Yuhei Inamori, Brett A Neilan, Brendan P Burns, Norio Sugiura
    Abstract:

    mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA Database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These strains possessed mlrA homologues, and the identities of the genes of MD-1 and Y2 with the corresponding MJ-PV exceeded 98% and 84%, respectively. On the other hand, the mlrA gene was not detected in laboratory strains of the closely related Sphingomonas spp. strains employing hemi-nested polymerase chain reaction detection using two primer sets. Although the microcystin-degrading bacteria were closely related strains, they did not cluster together as the same species. We can conclude that the mlrA gene is conserved in three different bacterial species, and it is unique to microcystin degraders but not to the genus Sphingomonas.