The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform

Ross C Hardison - One of the best experts on this subject based on the ideXlab platform.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian beta-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein-DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200-400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid epsilon-globin-luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 5' to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian β-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein‐DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200‐400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid e-globin‐luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 54 to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.

John D Jackson - One of the best experts on this subject based on the ideXlab platform.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian beta-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein-DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200-400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid epsilon-globin-luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 5' to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian β-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein‐DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200‐400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid e-globin‐luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 54 to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.

Qinglu Zhang - One of the best experts on this subject based on the ideXlab platform.

  • localization of pms3 a gene for photoperiod sensitive genic male sterility to a 28 4 kb DNA Fragment
    Molecular Genetics and Genomics, 2005
    Co-Authors: Dalong Guo, Qinglu Zhang
    Abstract:

    Photoperiod-sensitive genic male-sterile (PSGMS) rice, in which pollen fertility is regulated by day-length, originally arose as a natural mutant in the rice cultivar Nongken 58 (Oryza sativa ssp. japonica). Previous studies identified pms3 on chromosome 12 as the locus of the original PSGMS mutation. In this study we have assigned the pms3 locus to a 28.4-kb DNA Fragment by genetic and physical mapping. A cross between Nongken 58S (PSGMS line) and DH80 was used to produce an F2 population of about 7000 plants, from which 892 highly sterile individuals were obtained for recombination analysis. By analyzing recombination events in the sterile individuals using a total of 157 RFLP probes from a BAC contig covering the pms3 region, the pms3 locus was localized to a sub-region of less than 1.7 cM. Further analysis of recombination events using 49 additional probes isolated from this sub-region identified markers flanking the pms3 region on each side; these markers are only 28.4-kb apart. Sequence analysis of this Fragment predicted the presence of five ORFs, found high homology with two ESTs in public databases, and detected three SNPs between the mutant and the wild-type parents, which may be helpful for identifying a candidate gene for pms3.

Qingye Zhang - One of the best experts on this subject based on the ideXlab platform.

  • localization of pms3 a gene for photoperiod sensitive genic male sterility to a 28 4 kb DNA Fragment
    Molecular Genetics and Genomics, 2005
    Co-Authors: Qing Lu, X H Li, C G Xu, Qingye Zhang
    Abstract:

    Photoperiod-sensitive genic male-sterile (PSGMS) rice, in which pollen fertility is regulated by day-length, originally arose as a natural mutant in the rice cultivar Nongken 58 (Oryza sativa ssp. japonica). Previous studies identified pms3 on chromosome 12 as the locus of the original PSGMS mutation. In this study we have assigned the pms3 locus to a 28.4-kb DNA Fragment by genetic and physical mapping. A cross between Nongken 58S (PSGMS line) and DH80 was used to produce an F2 population of about 7000 plants, from which 892 highly sterile individuals were obtained for recombination analysis. By analyzing recombination events in the sterile individuals using a total of 157 RFLP probes from a BAC contig covering the pms3 region, the pms3 locus was localized to a sub-region of less than 1.7 cM. Further analysis of recombination events using 49 additional probes isolated from this sub-region identified markers flanking the pms3 region on each side; these markers are only 28.4-kb apart. Sequence analysis of this Fragment predicted the presence of five ORFs, found high homology with two ESTs in public databases, and detected three SNPs between the mutant and the wild-type parents, which may be helpful for identifying a candidate gene for pms3.

Webb Miller - One of the best experts on this subject based on the ideXlab platform.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian beta-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein-DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200-400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid epsilon-globin-luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 5' to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.

  • sequences within and flanking hypersensitive sites 3 and 2 of the β globin locus control region required for synergistic versus additive interaction with the e globin gene promoter
    Nucleic Acids Research, 1996
    Co-Authors: John D Jackson, Webb Miller, Ross C Hardison
    Abstract:

    The locus control region is required for high-level, position-independent expression of mammalian β-globin genes. It is marked by five major DNAse hypersensitive sites (HSs) in a 16 kb region of chromatin, and the protein‐DNA complexes that form these HSs may interact in a holocomplex that carries out the full function of the locus control region. Previous studies showed that a large rabbit DNA Fragment containing both HS2 and HS3 in their native sequence context and spacing produced a much larger increase in expression of a linked reporter gene than the sum of the largest effects observed with DNA Fragments containing HS2 or HS3 individually. To test whether this reflected a synergistic interaction between the 200‐400 bp cores of the HSs or if this effect required additional sequences outside the cores, combinations of different restriction Fragments containing HS2 or HS3 were tested for their ability to increase the expression of a hybrid e-globin‐luciferase reporter gene in transfected K562 cells. The results show that the human HS2 and HS3 cores do not interact either additively or synergistically with the reporter gene when juxtaposed, and separation by spacer DNA has little effect on their function. Fragments of human DNA containing cores plus flanking sequences for HS3 or HS2 show an additive effect in combination, whereas homologous Fragments of rabbit DNA containing HS3 and HS2 interact synergistically. At least part of this difference localizes to the rabbit DNA Fragment containing HS3, which can interact synergistically with the human DNA Fragment containing HS2. The region 54 to the HS3 core plays a role both in the cooperative interaction observed with the rabbit DNA Fragment and the domain-opening observed with the human DNA. A minor DNAse HS maps to this region, and the pattern of sequence conservation is consistent with some difference in function between species.