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  • high performance liquid chromatographic analysis of Docusate Sodium in soft gelatin capsules
    Journal of Pharmaceutical Sciences, 1992
    Co-Authors: David R Hogue, Joseph A Zimmardi, Kirit A Shah
    Abstract:

    A rapid and simple high‐performance liquid Chromatographic (HPLC) method for the analysis of Docusate Sodium in soft gelatin capsules was developed with progesterone as the internal standard. The method requires a reversed‐phase column and a pairedion technique to separate Docusate Sodium from other components. A C22 column was used with a mobile phase of acetonitrile: water (70:30) containing 0.005 M tetrabutylammonium phosphate. The flow rate was 1.8 mL/min, and the effluent was monitored at 214 nm. Docusate Sodium and progesterone had retention times of 4.5 and 6.8 min, respectively. The proposed HPLC method is linear, accurate, and precise. A mean assay value of 99.6% was obtained by the proposed method when five samples, each containing a composite of 10 capsules, were analyzed. The results obtained by the proposed HPLC, tetra‐n‐butylammonium iodide titration, and DSP XXII HPLC methods are compared.

  • High‐performance Liquid Chromatographic Analysis of Docusate Sodium in Soft Gelatin Capsules
    Journal of Pharmaceutical Sciences, 1992
    Co-Authors: David R Hogue, Joseph A Zimmardi, Kirit A Shah
    Abstract:

    A rapid and simple high‐performance liquid Chromatographic (HPLC) method for the analysis of Docusate Sodium in soft gelatin capsules was developed with progesterone as the internal standard. The method requires a reversed‐phase column and a pairedion technique to separate Docusate Sodium from other components. A C22 column was used with a mobile phase of acetonitrile: water (70:30) containing 0.005 M tetrabutylammonium phosphate. The flow rate was 1.8 mL/min, and the effluent was monitored at 214 nm. Docusate Sodium and progesterone had retention times of 4.5 and 6.8 min, respectively. The proposed HPLC method is linear, accurate, and precise. A mean assay value of 99.6% was obtained by the proposed method when five samples, each containing a composite of 10 capsules, were analyzed. The results obtained by the proposed HPLC, tetra‐n‐butylammonium iodide titration, and DSP XXII HPLC methods are compared.