The Experts below are selected from a list of 6330 Experts worldwide ranked by ideXlab platform
Jadwiga Chroboczek - One of the best experts on this subject based on the ideXlab platform.
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influenza recombinant vaccine matrix protein m1 on the platform of the adenovirus Dodecahedron
Vaccine, 2009Co-Authors: Antonina Naskalska, Ewa Szolajska, Jadwiga Chroboczek, Laurence Chaperot, Juliette Angel, Joel PlumasAbstract:We propose a novel influenza vaccine composed of the adenovirus Dodecahedron (Dd) as delivery platform carrying an internal influenza matrix protein M1. To attach the antigen to the vector we used WW domains interacting with Dd. Successful internalization of the Dd-M1WW complex was observed using biochemical and cell biology techniques. We show here that the complex of Dd with antigen is a potent activator of human myeloid dendritic cells (MDC), and that it is efficiently presented by MDC to M1-specific CD8+ T lymphocytes. These results show that proposed vaccine model is feasible and that adenovirus Dodecahedron is a potent delivery platform for foreign antigens to human cells.
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Protein transduction into human cells by adenovirus Dodecahedron using WW domains as universal adaptors.
The journal of gene medicine, 2006Co-Authors: A. Garcel, Evelyne Gout, J. Timmins, Jadwiga Chroboczek, Pascal FenderAbstract:Background Direct protein transduction is a recent technique that involves use of peptide vectors. In this study, we demonstrate that adenovirus Dodecahedron (Dd), a virus-like particle devoid of DNA and able to penetrate cells with high efficiency, can be used as a vector for protein delivery. Methods Taking advantage of Dd interaction with structural domains called WW, we have elaborated a universal adaptor to attach a protein of interest to this vector. Results A tandem of three WW structural domains derived from the Nedd4 protein enables the formation of stable complexes with Dd, without impairing its endocytosis efficiency. Our protein of interest fused to the triple WW linker is delivered by the Dodecahedron in 100% of cells in culture with on average more than ten million molecules per cell. Conclusion These data demonstrate the great potential of adenovirus Dodecahedron in combination with WW domains as a protein transduction vector. Copyright © 2006 John Wiley & Sons, Ltd.
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Adenovirus 3 penton Dodecahedron exhibits structural changes of the base on fibre binding.
The EMBO journal, 1996Co-Authors: Guy Schoehn, Pascal Fender, Jadwiga Chroboczek, Elizabeth A. HewatAbstract:It was recently shown that co-expression of adenovirus type 3 (Ad3) penton base and fibre in the baculovirus system produces dodecahedral particles, as does the expression of the penton base alone. The structure of both of these dodecahedral particles, with and without fibre, has been determined by cryoelectron microscopy and 3-dimensional reconstruction techniques to a resolution of 25 and 20 A, respectively. The general form of the penton base resembles that of the base protein in the recent reconstruction of adenovirus type 2. There is a remarkable difference in the penton base structure with and without the fibre. The five small protuberances on the outer surface of each base move away from the 5-fold axis by approximately 15 A when the fibre is present. These protuberances are of relatively low density and most probably represent a flexible loop possibly containing the RGD site involved in integrin binding. The fibre is apparently bound to the outer surface of the penton base, rather than inserted into it. The fibre is flexible and the shaft contains two distinct globular regions 26 A in diameter. The volume of the inner cavity of the Dodecahedron is 350 +/- 100 nm3. This small volume precludes the use of the inner cavity to house genetic information for gene therapy; however, the possibility remains of linking the gene to the Dodecahedron surface in the hope that it will be internalized with the Dodecahedron.
Elisa Prato - One of the best experts on this subject based on the ideXlab platform.
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Symplectic Toric Geometry and the Regular Dodecahedron
Journal of Mathematics, 2015Co-Authors: Elisa PratoAbstract:The regular Dodecahedron is the only simple polytope among the platonic solids which is not rational. Therefore, it corresponds neither to a symplectic toric manifold nor to a symplectic toric orbifold. In this paper, we associate to the regular Dodecahedron a highly singular space called symplectic toric quasifold.
Pascal Fender - One of the best experts on this subject based on the ideXlab platform.
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Use of Dodecahedron "VLPs" as an alternative to the whole adenovirus.
Methods in Molecular Biology, 2014Co-Authors: Pascal FenderAbstract:During human adenovirus type 3 (Ad3) infection, an excess of penton base and fiber proteins are produced. These form dodecahedral particles composed of 12 pentamers of penton base and 12 trimers of fiber protein. Beside this "natural" expression, the adenovirus Dodecahedron can be expressed in the heterologous baculovirus system in two forms: a fiber-devoid Dodecahedron made only of 12 penton bases (called base-Dodecahedron: Bs-Dd) and the fiber-containing Dodecahedron (called penton Dodecahedron: Pt-Dd). These particles partly mimic the adenoviral cellular entry pathway but are devoid of genetic information making them an unusual tool for basic research or applications. We report here how these particles are expressed and purified, the labeling method for trafficking studies as well as their use in molecular interaction studies. The potential of these particles for biotechnological applications is under evaluation, making their study a "niche" along side traditional adenoviral vectors.
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Protein transduction into human cells by adenovirus Dodecahedron using WW domains as universal adaptors.
The journal of gene medicine, 2006Co-Authors: A. Garcel, Evelyne Gout, J. Timmins, Jadwiga Chroboczek, Pascal FenderAbstract:Background Direct protein transduction is a recent technique that involves use of peptide vectors. In this study, we demonstrate that adenovirus Dodecahedron (Dd), a virus-like particle devoid of DNA and able to penetrate cells with high efficiency, can be used as a vector for protein delivery. Methods Taking advantage of Dd interaction with structural domains called WW, we have elaborated a universal adaptor to attach a protein of interest to this vector. Results A tandem of three WW structural domains derived from the Nedd4 protein enables the formation of stable complexes with Dd, without impairing its endocytosis efficiency. Our protein of interest fused to the triple WW linker is delivered by the Dodecahedron in 100% of cells in culture with on average more than ten million molecules per cell. Conclusion These data demonstrate the great potential of adenovirus Dodecahedron in combination with WW domains as a protein transduction vector. Copyright © 2006 John Wiley & Sons, Ltd.
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Adenovirus 3 penton Dodecahedron exhibits structural changes of the base on fibre binding.
The EMBO journal, 1996Co-Authors: Guy Schoehn, Pascal Fender, Jadwiga Chroboczek, Elizabeth A. HewatAbstract:It was recently shown that co-expression of adenovirus type 3 (Ad3) penton base and fibre in the baculovirus system produces dodecahedral particles, as does the expression of the penton base alone. The structure of both of these dodecahedral particles, with and without fibre, has been determined by cryoelectron microscopy and 3-dimensional reconstruction techniques to a resolution of 25 and 20 A, respectively. The general form of the penton base resembles that of the base protein in the recent reconstruction of adenovirus type 2. There is a remarkable difference in the penton base structure with and without the fibre. The five small protuberances on the outer surface of each base move away from the 5-fold axis by approximately 15 A when the fibre is present. These protuberances are of relatively low density and most probably represent a flexible loop possibly containing the RGD site involved in integrin binding. The fibre is apparently bound to the outer surface of the penton base, rather than inserted into it. The fibre is flexible and the shaft contains two distinct globular regions 26 A in diameter. The volume of the inner cavity of the Dodecahedron is 350 +/- 100 nm3. This small volume precludes the use of the inner cavity to house genetic information for gene therapy; however, the possibility remains of linking the gene to the Dodecahedron surface in the hope that it will be internalized with the Dodecahedron.
Bholanath Mandal - One of the best experts on this subject based on the ideXlab platform.
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Eigensolutions of Dodecahedron graphs
Chemical Physics Letters, 2014Co-Authors: Piyali Ghosh, Somnath Karmakar, Bholanath MandalAbstract:Abstract Eigensolutions of 20-vertex cage (i.e. Dodecahedron) have been determined with the use of fivefold rotational symmetry. For homo-Dodecahedron the eigensolutions become analytical but for the hetero-Dodecahedron having two different types of atoms ( ( C , N ) , ( C , B ) , ( B , N ) ) the eigensolutions are found to be factored out into five 4-degree polynomials with one corresponding to nondegenerate and other four corresponding to two degenerate eigensolutions. Eigenspectra and total π-electron energies of homo- and hetero-Dodecahedron graphs have been calculated.
Shuhong Yu - One of the best experts on this subject based on the ideXlab platform.
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monodisperse cubic pyrite nis2 Dodecahedrons and microspheres synthesized by a solvothermal process in a mixed solvent thermal stability and magnetic properties
CrystEngComm, 2009Co-Authors: Shiliu Yang, Shuhong YuAbstract:Monodisperse cubic pyrite NiS2 Dodecahedrons and microspheres with a diameter of 6 µm have been synthesized by a solvothermal approach in ethylenediamine–glycol mixed solvent at 200 °C using NiCl2·6H2O and sulfur as precursors. The morphology and the phase transformation of the products were found to be strongly dependent on the reaction temperature, reaction time, and the volume ratio of ethylenediamine to glycol. The thermal stability of the pyrite NiS2 Dodecahedrons has been studied and they can act as excellent templates for synthesis of porous NiO microspheres by calcination in air. The NiS2 Dodecahedrons show antiferromagnetic properties between 10 and 300 K. The present solvothermal approach in a mixed solvent system may allow to synthesize other metal chalcogenides with unique shapes and structures.