The Experts below are selected from a list of 1971 Experts worldwide ranked by ideXlab platform
Chunlan Zhang - One of the best experts on this subject based on the ideXlab platform.
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Sheep skeletal muscle transcriptome analysis reveals muscle growth regulatory lncRNAs
PeerJ Inc., 2018Co-Authors: Tianle Chao, Lei Hou, Jin Wang, Chunlan Zhang, Guizhi Wang, Jianmin WangAbstract:As widely distributed domestic animals, Sheep are an important species and the source of mutton. In this study, we aimed to evaluate the regulatory lncRNAs associated with muscle growth and development between high production mutton Sheep (Dorper Sheep and Qianhua Mutton Merino Sheep) and low production mutton Sheep (Small-tailed Han Sheep). In total, 39 lncRNAs were found to be differentially expressed. Using co-expression analysis and functional annotation, 1,206 co-expression interactions were found between 32 lncRNAs and 369 genes, and 29 of these lncRNAs were found to be associated with muscle development, metabolism, cell proliferation and apoptosis. lncRNA–mRNA interactions revealed 6 lncRNAs as hub lncRNAs. Moreover, three lncRNAs and their associated co-expressed genes were demonstrated by cis-regulatory gene analyses, and we also found a potential regulatory relationship between the pseudogene lncRNA LOC101121401 and its parent gene FTH1. This study provides a genome-wide resolution of lncRNA and mRNA regulation in muscles from mutton Sheep
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identification and classification of new transcripts in Dorper and small tailed han Sheep skeletal muscle transcriptomes
PLOS ONE, 2016Co-Authors: Tianle Chao, Lei Hou, Guizhi Wang, Jianmin Wang, Zhaohua Liu, Chunlan ZhangAbstract:High-throughput mRNA sequencing enables the discovery of new transcripts and additional parts of incompletely annotated transcripts. Compared with the human and cow genomes, the reference annotation level of the Sheep genome is still low. An investigation of new transcripts in Sheep skeletal muscle will improve our understanding of muscle development. Therefore, applying high-throughput sequencing, two cDNA libraries from the biceps brachii of small-tailed Han Sheep and Dorper Sheep were constructed, and whole-transcriptome analysis was performed to determine the unknown transcript catalogue of this tissue. In this study, 40,129 transcripts were finally mapped to the Sheep genome. Among them, 3,467 transcripts were determined to be unannotated in the current reference Sheep genome and were defined as new transcripts. Based on protein-coding capacity prediction and comparative analysis of sequence similarity, 246 transcripts were classified as portions of unannotated genes or incompletely annotated genes. Another 1,520 transcripts were predicted with high confidence to be long non-coding RNAs. Our analysis also revealed 334 new transcripts that displayed specific expression in ruminants and uncovered a number of new transcripts without intergenus homology but with specific expression in Sheep skeletal muscle. The results confirmed a complex transcript pattern of coding and non-coding RNA in Sheep skeletal muscle. This study provided important information concerning the Sheep genome and transcriptome annotation, which could provide a basis for further study.
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characterization and comparative analyses of muscle transcriptomes in Dorper and small tailed han Sheep using rna seq technique
PLOS ONE, 2013Co-Authors: Chunlan Zhang, Guizhi Wang, Jianmin Wang, Zhaohuan Liu, Cunxian ChenAbstract:The Sheep is an important domestic animal and model for many types of medically relevant research. An investigation of gene expression in ovine muscle would significantly advance our understanding of muscle growth. RNA-seq is a recently developed analytical approach for transcriptome profiling via high-throughput sequencing. Although RNA-seq has been recently applied to a wide variety of organisms, few RNA-seq studies have been conducted in livestock, particularly in Sheep. In this study, two cDNA libraries were constructed from the biceps brachii of one Small-tailed Han Sheep (SH) and one Dorper Sheep (DP). The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics. For the SH and DP libraries, we obtained a total of 50,264,608 and 52,794,216 high quality reads, respectively. Approximately two-thirds of the reads could be mapped to the Sheep genome. In addition, 40,481 and 38,851 potential coding single nucleotide polymorphisms (cSNPs) were observed, respectively, of which a total of 59,139 cSNP coordinates were different between the two samples. Up to 5,116 and 5,265 respective reference genes had undergone 13,827 and 15,684 alternative splicing events. A total of 6,989 reference genes were extended at the 5’, 3’ or both ends, and 123,678 novel transcript units were found. A total of 1,300 significantly differentially expressed genes were identified between the two libraries. These results suggest that there are many differences in the muscle transcriptomes between these two animals. This study addresses a preliminary analysis and offers a foundation for future genomic research in the Sheep.
Desalegn Ayichew Walle - One of the best experts on this subject based on the ideXlab platform.
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Dorper Sheep cross breeding with Indigenous Sheep breed in Ethiopia
Journal of Applied and Advanced Research, 2019Co-Authors: Desalegn Ayichew WalleAbstract:The objective of this review paper was to review Dorper Sheep cross breeding with indigenous Sheep breed in Ethiopia. The paper reviewed and discussed the history of Dorper breed introduction, research and development efforts in crossbreeding and their performance under on-station and on-farm management. Formal survey on farmers perception on Dorper cross Sheep indicated that the crossbred Dorper Sheep have non selective feed behavior and excellent in meat production under farmer`s management. On other study showed that Dorper Sheep was not meet farmer interest in breeding Sheep aspect because of black coat color. The performance of indigenous Sheep and their crosses with Dorper varied as per the location, management, farming conditions and percentage of exotic blood level inheritance. Different research output by team of researchers on farm and on station performance evaluation of Dorper indicated that crossbreds often outperformed their local contemporaries . Under on farm condition, body weight at different ages was significantly higher in 50% Dorper crosses as compared to their 25% and75% counter parts.On station birth weight of Dorper (3.39 3.8 kg) better than crossbreed (3.0 3.24 ± 0.04 kg) and local Sheep (2.36 2.77 kg), respectively.While the mean weaning weight (14 16 kg) and yearling weight (26.95 32.43 ± 0.46 kg) of 50% Dorper crossbreed was better than indigenous Sheep breeds. However, crossbred ewes and local Sheep breeds did not differ in litter size. Dorper crosses with Afar under on station is not economically important due to lower weight in all aspects. Crossbreeding programs of Dorper with indigenous Sheep require strong research and development support from public service and non-governmental institutions for sustainable design, optimization, and implementation in clearly defined production environments.
Jianmin Wang - One of the best experts on this subject based on the ideXlab platform.
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Sheep skeletal muscle transcriptome analysis reveals muscle growth regulatory lncRNAs
PeerJ Inc., 2018Co-Authors: Tianle Chao, Lei Hou, Jin Wang, Chunlan Zhang, Guizhi Wang, Jianmin WangAbstract:As widely distributed domestic animals, Sheep are an important species and the source of mutton. In this study, we aimed to evaluate the regulatory lncRNAs associated with muscle growth and development between high production mutton Sheep (Dorper Sheep and Qianhua Mutton Merino Sheep) and low production mutton Sheep (Small-tailed Han Sheep). In total, 39 lncRNAs were found to be differentially expressed. Using co-expression analysis and functional annotation, 1,206 co-expression interactions were found between 32 lncRNAs and 369 genes, and 29 of these lncRNAs were found to be associated with muscle development, metabolism, cell proliferation and apoptosis. lncRNA–mRNA interactions revealed 6 lncRNAs as hub lncRNAs. Moreover, three lncRNAs and their associated co-expressed genes were demonstrated by cis-regulatory gene analyses, and we also found a potential regulatory relationship between the pseudogene lncRNA LOC101121401 and its parent gene FTH1. This study provides a genome-wide resolution of lncRNA and mRNA regulation in muscles from mutton Sheep
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identification and classification of new transcripts in Dorper and small tailed han Sheep skeletal muscle transcriptomes
PLOS ONE, 2016Co-Authors: Tianle Chao, Lei Hou, Guizhi Wang, Jianmin Wang, Zhaohua Liu, Chunlan ZhangAbstract:High-throughput mRNA sequencing enables the discovery of new transcripts and additional parts of incompletely annotated transcripts. Compared with the human and cow genomes, the reference annotation level of the Sheep genome is still low. An investigation of new transcripts in Sheep skeletal muscle will improve our understanding of muscle development. Therefore, applying high-throughput sequencing, two cDNA libraries from the biceps brachii of small-tailed Han Sheep and Dorper Sheep were constructed, and whole-transcriptome analysis was performed to determine the unknown transcript catalogue of this tissue. In this study, 40,129 transcripts were finally mapped to the Sheep genome. Among them, 3,467 transcripts were determined to be unannotated in the current reference Sheep genome and were defined as new transcripts. Based on protein-coding capacity prediction and comparative analysis of sequence similarity, 246 transcripts were classified as portions of unannotated genes or incompletely annotated genes. Another 1,520 transcripts were predicted with high confidence to be long non-coding RNAs. Our analysis also revealed 334 new transcripts that displayed specific expression in ruminants and uncovered a number of new transcripts without intergenus homology but with specific expression in Sheep skeletal muscle. The results confirmed a complex transcript pattern of coding and non-coding RNA in Sheep skeletal muscle. This study provided important information concerning the Sheep genome and transcriptome annotation, which could provide a basis for further study.
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characterization and comparative analyses of muscle transcriptomes in Dorper and small tailed han Sheep using rna seq technique
PLOS ONE, 2013Co-Authors: Chunlan Zhang, Guizhi Wang, Jianmin Wang, Zhaohuan Liu, Cunxian ChenAbstract:The Sheep is an important domestic animal and model for many types of medically relevant research. An investigation of gene expression in ovine muscle would significantly advance our understanding of muscle growth. RNA-seq is a recently developed analytical approach for transcriptome profiling via high-throughput sequencing. Although RNA-seq has been recently applied to a wide variety of organisms, few RNA-seq studies have been conducted in livestock, particularly in Sheep. In this study, two cDNA libraries were constructed from the biceps brachii of one Small-tailed Han Sheep (SH) and one Dorper Sheep (DP). The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics. For the SH and DP libraries, we obtained a total of 50,264,608 and 52,794,216 high quality reads, respectively. Approximately two-thirds of the reads could be mapped to the Sheep genome. In addition, 40,481 and 38,851 potential coding single nucleotide polymorphisms (cSNPs) were observed, respectively, of which a total of 59,139 cSNP coordinates were different between the two samples. Up to 5,116 and 5,265 respective reference genes had undergone 13,827 and 15,684 alternative splicing events. A total of 6,989 reference genes were extended at the 5’, 3’ or both ends, and 123,678 novel transcript units were found. A total of 1,300 significantly differentially expressed genes were identified between the two libraries. These results suggest that there are many differences in the muscle transcriptomes between these two animals. This study addresses a preliminary analysis and offers a foundation for future genomic research in the Sheep.
S. Kusza - One of the best experts on this subject based on the ideXlab platform.
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Examination of microsatellite markers of Dorper Sheep breed
Acta Agraria Debreceniensis, 2016Co-Authors: N. Csizmar, András Jávor, S. KuszaAbstract:Number of not woolly and molty Sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the country. The Dorper Sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.
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effects of behavioral reactivity on production and reproduction traits in Dorper Sheep breed
Journal of Veterinary Behavior-clinical Applications and Research, 2015Co-Authors: Dinu Gavojdian, Ludovic Toma Cziszter, Csilla Budai, S. KuszaAbstract:Abstract The aim of the current research was to evaluate the effects of behavioral reactivity on production and reproduction performance in Dorper Sheep, a meat breed. Temperament of animals was subjectively evaluated using a 5-point scoring system at weighing, during which Sheep spent 30 seconds on the scale. The heritability of temperament was low (0.10 ± 0.03). Estimated phenotypic ( r p ) and genetic ( r g ) correlations between temperament and litter size in ewes were negative and negligible ( P > 0.05) at −0.06 ± 0.18 and −0.08 ± 0.09, respectively. Significant genetic correlations were found between lamb temperament and preweaning growth rate ( r g = −0.44 ± 0.07, P ≤ 0.05) and postweaning growth till the age of 120 days ( r g = −0.52 ± 0.08, P ≤ 0.05). Significant genetic correlations were found between lamb temperament and growth rates during their first 3 months of age. As a result, selection for calm temperaments should translate to increased growth rates of lambs. Negative correlations between temperament and production traits, both in ewes and lambs, suggested that selection against animals that are highly reactive to improve welfare and ease of handling would not have detrimental impacts on productivity.
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Reproduction Efficiency and Health Traits in Dorper, White Dorper, and Tsigai Sheep Breeds under Temperate European Conditions
Asian-Australasian journal of animal sciences, 2015Co-Authors: Dinu Gavojdian, C. Budai, Ludovic Toma Cziszter, N. Csizmar, András Jávor, S. KuszaAbstract:The objective of the current pilot study was to evaluate the reproductive performance and health indicators in Dorper, White Dorper, and Tsigai breed ewes managed semi-intensively under European temperate conditions. A total of 544 ewe-year units were observed, with ewes (ranging from 1.5 to 8 years of age) managed under identical rearing conditions for a period of two consecutive production cycles (2012 through 2013 and 2013 through 2014). In general, significant (p≤0.001) genotype-related disparities were found in occurrence rates for all health parameters taken into study. Clinical mastitis incidence was significantly lower (p≤0.05) in Dorper (9.4%) and White Dorper (10.8%) breeds compared to that of Tsigai ewes (17.4%). Significant differences (p≤0.05) for lameness were found between Dorper and Tsigai breeds, with occurrence rates of 8.0% and 2.9%, respectively. Incidence for pneumonia and abortion was not influenced (p>0.05) by the ewes' genotype. Litter size was significantly lower (p≤0.05) in White Dorper breed than for Dorper and Tsigai ewes, of 1.21, 1.40, and 1.45, respectively. Conception rates and lambs survival were not affected (p>0.05) by genotype. Results suggest that South African Dorper and White Dorper Sheep breeds have adapted well to the
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performance and adaptability of the Dorper Sheep breed under hungarian and romanian rearing conditions
Scientific Papers: Animal Science and Biotechnologies, 2013Co-Authors: Csilla Budai, Dinu Gavojdian, Ludovic Toma Cziszter, S. Kusza, Andras Kovacs, Felician Negrut, Janos Olah, András JávorAbstract:Dorper (DO) is a meat-specialized hair composite breed, intensively selected for growth rates, carcass quality, unselective grazing. The aim of the current comparative study was to evaluate the adaptability and performance of DO breed under Hungarian and Romanian rearing conditions. DO breed was introduced into Hungary to Debrecen University in 2008. Under Hungarian rearing conditions the DO breed maintained its non-seasonal reproduction and reproductive precocity, with maiden ewes being put to ram starting the age of 9 months. Lamb crops of 2 weaned per year are common under proper management and feeding conditions. In Romania DO breed was introduced starting 2007, with the heard-book and genetic improvement plan of the breed being established in 2009. Under Romanian farming conditions the DO rams are usually used as terminal sires which are crossed with indigenous Turcana and Tsigai breeds, being preferred by the farmers to sire the crossbreds because of the high growth rates and nonselective grazing of the crossbreds. Fertility of DO ewes and survival rates of the DO sired lambs until weaning were not affected when compared to native Turcana breed. It was concluded that DO breed has adapted and performs extremely well under both Hungarian and Romanian rearing conditions.
Guizhi Wang - One of the best experts on this subject based on the ideXlab platform.
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Sheep skeletal muscle transcriptome analysis reveals muscle growth regulatory lncRNAs
PeerJ Inc., 2018Co-Authors: Tianle Chao, Lei Hou, Jin Wang, Chunlan Zhang, Guizhi Wang, Jianmin WangAbstract:As widely distributed domestic animals, Sheep are an important species and the source of mutton. In this study, we aimed to evaluate the regulatory lncRNAs associated with muscle growth and development between high production mutton Sheep (Dorper Sheep and Qianhua Mutton Merino Sheep) and low production mutton Sheep (Small-tailed Han Sheep). In total, 39 lncRNAs were found to be differentially expressed. Using co-expression analysis and functional annotation, 1,206 co-expression interactions were found between 32 lncRNAs and 369 genes, and 29 of these lncRNAs were found to be associated with muscle development, metabolism, cell proliferation and apoptosis. lncRNA–mRNA interactions revealed 6 lncRNAs as hub lncRNAs. Moreover, three lncRNAs and their associated co-expressed genes were demonstrated by cis-regulatory gene analyses, and we also found a potential regulatory relationship between the pseudogene lncRNA LOC101121401 and its parent gene FTH1. This study provides a genome-wide resolution of lncRNA and mRNA regulation in muscles from mutton Sheep
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identification and classification of new transcripts in Dorper and small tailed han Sheep skeletal muscle transcriptomes
PLOS ONE, 2016Co-Authors: Tianle Chao, Lei Hou, Guizhi Wang, Jianmin Wang, Zhaohua Liu, Chunlan ZhangAbstract:High-throughput mRNA sequencing enables the discovery of new transcripts and additional parts of incompletely annotated transcripts. Compared with the human and cow genomes, the reference annotation level of the Sheep genome is still low. An investigation of new transcripts in Sheep skeletal muscle will improve our understanding of muscle development. Therefore, applying high-throughput sequencing, two cDNA libraries from the biceps brachii of small-tailed Han Sheep and Dorper Sheep were constructed, and whole-transcriptome analysis was performed to determine the unknown transcript catalogue of this tissue. In this study, 40,129 transcripts were finally mapped to the Sheep genome. Among them, 3,467 transcripts were determined to be unannotated in the current reference Sheep genome and were defined as new transcripts. Based on protein-coding capacity prediction and comparative analysis of sequence similarity, 246 transcripts were classified as portions of unannotated genes or incompletely annotated genes. Another 1,520 transcripts were predicted with high confidence to be long non-coding RNAs. Our analysis also revealed 334 new transcripts that displayed specific expression in ruminants and uncovered a number of new transcripts without intergenus homology but with specific expression in Sheep skeletal muscle. The results confirmed a complex transcript pattern of coding and non-coding RNA in Sheep skeletal muscle. This study provided important information concerning the Sheep genome and transcriptome annotation, which could provide a basis for further study.
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characterization and comparative analyses of muscle transcriptomes in Dorper and small tailed han Sheep using rna seq technique
PLOS ONE, 2013Co-Authors: Chunlan Zhang, Guizhi Wang, Jianmin Wang, Zhaohuan Liu, Cunxian ChenAbstract:The Sheep is an important domestic animal and model for many types of medically relevant research. An investigation of gene expression in ovine muscle would significantly advance our understanding of muscle growth. RNA-seq is a recently developed analytical approach for transcriptome profiling via high-throughput sequencing. Although RNA-seq has been recently applied to a wide variety of organisms, few RNA-seq studies have been conducted in livestock, particularly in Sheep. In this study, two cDNA libraries were constructed from the biceps brachii of one Small-tailed Han Sheep (SH) and one Dorper Sheep (DP). The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics. For the SH and DP libraries, we obtained a total of 50,264,608 and 52,794,216 high quality reads, respectively. Approximately two-thirds of the reads could be mapped to the Sheep genome. In addition, 40,481 and 38,851 potential coding single nucleotide polymorphisms (cSNPs) were observed, respectively, of which a total of 59,139 cSNP coordinates were different between the two samples. Up to 5,116 and 5,265 respective reference genes had undergone 13,827 and 15,684 alternative splicing events. A total of 6,989 reference genes were extended at the 5’, 3’ or both ends, and 123,678 novel transcript units were found. A total of 1,300 significantly differentially expressed genes were identified between the two libraries. These results suggest that there are many differences in the muscle transcriptomes between these two animals. This study addresses a preliminary analysis and offers a foundation for future genomic research in the Sheep.