The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform

Paul A. Webley - One of the best experts on this subject based on the ideXlab platform.

  • mechanical cell disruption for lipid extraction from microalgal biomass
    Bioresource Technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65ultrasonication was directly proportional to power level and followed a parabolic relationship with initial cell concentration, while that for HPH was directly proportional to operating pressure and inversely proportional to initial cell concentration. Mean disruption rate constant for HPH was approximately seven times that for ultrasonication. Mean disruption rate constant for TS cells was roughly 20% higher than that for C sp. cells. Subjecting TS culture to cell disruption prior to lipid extraction resulted in 5-8-fold increase in lipid yield and 3-5-fold increase in triglyceride yield.

  • Mechanical cell disruption for lipid extraction from microalgal biomass.
    Bioresource technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65

Ronald Halim - One of the best experts on this subject based on the ideXlab platform.

  • mechanical cell disruption for lipid extraction from microalgal biomass
    Bioresource Technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65ultrasonication was directly proportional to power level and followed a parabolic relationship with initial cell concentration, while that for HPH was directly proportional to operating pressure and inversely proportional to initial cell concentration. Mean disruption rate constant for HPH was approximately seven times that for ultrasonication. Mean disruption rate constant for TS cells was roughly 20% higher than that for C sp. cells. Subjecting TS culture to cell disruption prior to lipid extraction resulted in 5-8-fold increase in lipid yield and 3-5-fold increase in triglyceride yield.

  • Mechanical cell disruption for lipid extraction from microalgal biomass.
    Bioresource technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65

Thusitha W. T. Rupasinghe - One of the best experts on this subject based on the ideXlab platform.

  • mechanical cell disruption for lipid extraction from microalgal biomass
    Bioresource Technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65ultrasonication was directly proportional to power level and followed a parabolic relationship with initial cell concentration, while that for HPH was directly proportional to operating pressure and inversely proportional to initial cell concentration. Mean disruption rate constant for HPH was approximately seven times that for ultrasonication. Mean disruption rate constant for TS cells was roughly 20% higher than that for C sp. cells. Subjecting TS culture to cell disruption prior to lipid extraction resulted in 5-8-fold increase in lipid yield and 3-5-fold increase in triglyceride yield.

  • Mechanical cell disruption for lipid extraction from microalgal biomass.
    Bioresource technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65

Dedreia Tull - One of the best experts on this subject based on the ideXlab platform.

  • mechanical cell disruption for lipid extraction from microalgal biomass
    Bioresource Technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65ultrasonication was directly proportional to power level and followed a parabolic relationship with initial cell concentration, while that for HPH was directly proportional to operating pressure and inversely proportional to initial cell concentration. Mean disruption rate constant for HPH was approximately seven times that for ultrasonication. Mean disruption rate constant for TS cells was roughly 20% higher than that for C sp. cells. Subjecting TS culture to cell disruption prior to lipid extraction resulted in 5-8-fold increase in lipid yield and 3-5-fold increase in triglyceride yield.

  • Mechanical cell disruption for lipid extraction from microalgal biomass.
    Bioresource technology, 2013
    Co-Authors: Ronald Halim, Thusitha W. T. Rupasinghe, Dedreia Tull, Paul A. Webley
    Abstract:

    Cell disruption is an integral part of the Downstream Operation required to produce biodiesel from microalgae. This study investigated the use of ultrasonication and high-pressure homogenization (HPH) as cell disruption methods for two microalgal species, Tetraselmis suecica (TS) and Chlorococcum sp. (C sp.). The kinetics of cell disruption followed a first-order model (0.65

Reiner Luttmann - One of the best experts on this subject based on the ideXlab platform.

  • development and monitoring of an integrated bioprocess for production of a potential malaria vaccine with pichia pastoris
    Journal of Process Control, 2015
    Co-Authors: Sven-oliver Borchert, Jessica Paul, Fabian Schuetzmeier, Tobias Voß, Gesine Cornelissen, Reiner Luttmann
    Abstract:

    An engineered variant of the yeast Pichia pastoris was used to produce the potential malaria vaccine D1M1. This paper describes the implementation of an expanded bed adsorption chromatography step into a sequential cultivation process of the yeast in order to link protein expression, cell release, and product capture of the secreted protein in a fully automated plant. The process is operated with a semi-continuous strategy for an integrated production. Process improvements have been achieved using design of experiments. Resulting purities of product achieved up to 87% with recoveries of 51% in a single Downstream Operation. Furthermore, a multivariate analysis of historical batch data was used to develop a golden batch model for process monitoring.

  • Development of an Integrated Bioprocess for production of potential Malaria vaccines with Pichia pastoris
    IFAC Proceedings Volumes, 2013
    Co-Authors: Sven-oliver Borchert, Jessica Paul, Fabian Schuetzmeier, Tobias Voß, Reiner Luttmann, Gesine Cornelissen
    Abstract:

    Abstract Pichia pastoris was used for the expression of an artificial fusion protein D1M1 – a potential Malaria vaccine. A fully automated integrated bioprocess was set up which combined upstream and Downstream Operations and has been performed in alternating fed batch cycles with parallel protein capture by Expanded Bed Adsorption Chromatography. Design of Experiments was used to study the influence of procedural factors on protein recovery and purity. As a result it was achieved to purify the protein to a purity of up to 87 % and a recovery of 51 % was reached in a single Downstream Operation.