The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Shuichi Shoji - One of the best experts on this subject based on the ideXlab platform.
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A high-resolution passive Droplet-Phase sample sorter using multi-stage Droplet transfer
RSC Advances, 2017Co-Authors: Dong Hyun Yoon, Daiki Tanaka, Tetsushi Sekiguchi, Shuichi ShojiAbstract:A fully passive volume-dependent Droplet sorter is presented in this research. Repeated and multiple on-rail transfer of microDroplets in a cascade channel resulted in high resolution sorting of Droplet-Phase samples. MicroDroplets with volumes of 0.89–3.26 nl were successfully sorted in eight steps, and a maximum sorting resolution of about 0.21 nl was obtained over three transfer stages. Line rails and dot rails employed in the cascading channels allowed volume-dependent Droplet transfer, and shape recovery of the transferred Droplet for the repeatable transfer, respectively. This simple device and method can be applied to Droplet-based chemical or biological research.
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Hydrodynamic on-rail Droplet pass filter for fully passive sorting of Droplet-Phase samples
RSC Advances, 2014Co-Authors: Dong Hyun Yoon, Tetsushi Sekiguchi, Satoshi Numakunai, Asahi Nakahara, Shuichi ShojiAbstract:A hydrodynamic Droplet pass filter for Droplet-Phase sample sorting was developed in this study. Using only groove rails, without additional components or external controls, Droplets were sorted based on their physical properties. This is the first report of a Droplet pass filter used for effective sorting, and the sorting structure provides a novel fluidic component for fluidic circuits for many applications. Depending on the number of rails, we obtained high-pass, low-pass, band-pass, and multi band-pass filters for sorting Droplet samples, and their filtration performance was controlled by varying the dimensions of the rail structures. We evaluated in detail the effect of the rail width on sorting, threshold size of Droplets sorted into each rail, and capillary number-dependent sample sorting. Furthermore, band-pass Droplet sorting, useful for sample quantification, was provided, and multi-step rail ways allowed multi band-pass Droplet sorting that was independent of flow conditions. The proposed sorting method does not require any external systems or skilled operation, and thus, it is expected that the device can contribute to on-site sample treatment and analysis in various fields such as medical care or the military.
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Digital sorting of flow-through Droplet Phase microfluid
Japanese Journal of Applied Physics, 2003Co-Authors: Jeung Sang Go, Shuichi ShojiAbstract:The in-plane digital sorting of the multiPhase microfluid flow is presented in the laminar flow regime. It is achieved by manipulating the pitch of alternate microfluid flow and delay distance resulting from passing through special microchannels. The theoretical analysis derives delay distance. For experimental evaluation, a characteristic in-plane microchannel is fabricated by using micromachining. The pitch of the alternate microfluid flow is controlled. Digital sorting is demonstrated by using the immiscible Droplet Phase fluids. Also, the dynamic behavior of the Droplet Phase microfluids is measured by the particle image velocimetry (micro-PIV) and characterized qualitatively and qualitatively.
A. Hoell - One of the best experts on this subject based on the ideXlab platform.
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Effect of the Cl− content on the formation and dissolution of precipitates in a soda–lime–silica glass
Journal of Non-crystalline Solids, 2001Co-Authors: R. Kranold, M. Kammel, A. HoellAbstract:Abstract The glass with composition 13Na2O–11CaO–76SiO2 (mol%) undergoes subliquidus Phase separation via a binodal mechanism. Below the binodal temperature, Tb, the glass separates into two amorphous Phases, silica-enriched Droplets and a silica-poor matrix. Small-angle X-ray scattering (SAXS) is used to study the formation process of the Droplet Phase at 600 °C, as well as, the inverse process where the precipitates dissolve after an increase in temperature. A small amount of 0.47 mol% Cl− in the glass causes a drastic change of the kinetics and thermodynamics of the Phase separation process in comparison with the pure glass: the Phase separation is accelerated and the maximum volume fraction of the silica Droplet Phase is increased. The results of dissolution experiments demonstrate that due to the Cl− content Tb increases by about 60 K. So, the main effect of Cl− in the glass is interpreted as a shift of the miscibility gap to higher temperatures resulting from a higher thermodynamic driving force.
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Reversion of precipitates in Phase separated soda lime silica glass
Journal of Applied Crystallography, 2000Co-Authors: R. Kranold, A. Hoell, M. Kammel, U. Lembke, G. Von KrosigkAbstract:The glass with composition 13Na 2 O-11CaO-76SiO 2 (mol%) undergoes subliquidus Phase separation via a binodal mechanism. At temperatures below the binodal temperature, T b =978 K, the glass separates into two amorphous Phases, silica-enriched Droplets and a silica-poor matrix. Small-angle X-ray scattering was used to study the formation of the Droplet Phase at 923 K as well as the process of reversion where the precipitates dissolve after an increase in temperature. After a heat treatment of 48 h at 923 K the system is in the stage of coarsening. However, the equilibrium volume fraction of the Droplet Phase, W c =0.073, has not yet precipitated. The reversion of the Droplets was studied at T=983 K and T = 963 K. For T>T b , the precipitates dissolve completely by an interdiffusion process resulting in a decrease of the silica concentration of the Droplets, while the Phase boundary is preserved over long times. For T
Gintautas Miliauskas - One of the best experts on this subject based on the ideXlab platform.
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Experimental Investigation of Water Droplet Phase Change in Humidi-fied Air Flow
Mechanics, 2020Co-Authors: V. Ramanauskas, Linas Paukštaitis, Gintautas Miliauskas, Egidijus PuidaAbstract:An experimental research method and an analysis of the results of a water Droplet Phase change in the additionally humidified air flow are presented. The diagrams of variation of the equivalent diameter of convectively heated water Droplets are presented and analysed. The influence of initial water temperature and additionally humidifying air flow on the Phase change of a Droplet in transient regime is experimentally substantiated.
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The water Droplets dynamics and Phase transformations in biofuel flue gases flow
International Journal of Heat and Mass Transfer, 2019Co-Authors: V. Ramanauskas, Gintautas MiliauskasAbstract:Abstract This work presents the Phase transformations and movement dynamics in a case of complex transfer processes self-interaction on sprayed water Droplets in biofuel furnace flue gases flow. The Droplet Phase transformation cycle is defined according to a numerical iterative scheme which is created by heat flow balance condition onto the Droplet surface, where combined solutions for the tasks of external heat transfer and Phase change as well as internal heat transfer in a Droplet, are designed. The flue gases velocity, temperature and Droplet dispersity are named as defining factors of Droplets trace to full evaporation in flue gases at biofuel furnace. Relevant secondary factors are recognized as sprayed water temperature, initial velocity provided for Droplets, humidity of flue gases and specifics of Droplets heat transfer. It is based on, that boundary conditions, which ensures the fastest and the slowest water Droplets evaporation, do not define the marginal trace of Droplets till evaporation. In biofuel flue gases flow at Droplet Phase transformation regimes cycle two, practically equivalent periods are distinguished. In the first period significant is Droplet growth due to influence of condensation, warming water expansion and Droplets slipping decrease in flue gases. In the second period the trace of Droplets is already defined by flue gases flow velocity. The combined Droplet heating process is more significant for larger Droplets, while for smaller than 70 µm diameter Droplets impact of thermal radiation is neglect.
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Peculiarities of the transit Phase transformation regime for water Droplets that are slipping in humid gas
International Journal of Heat and Mass Transfer, 2016Co-Authors: Gintautas Miliauskas, Monika Maziukienė, V. RamanauskasAbstract:Abstract Water Droplets Phase transformation cycle in humid gas is defined by time moments of Droplets generation and evaporation. Transit and equilibrium evaporation regimes are provided. An exclusive attention is payed for Droplet heat and mass transfer processes analysis in transit Phase transformation regime when providing condensing and transit evaporation regimes in it. Specific transfer processes regularities were highlighted for each Droplet Phase transformation regime and original their interpretation according to Droplet thermal and energy state variation is presented. Droplet slipping in gas impact for its Phase transformation cycle is evaluated. Essential impact of rising water circulation at the Droplet slipping in gas for condensation Phase transformation regime duration that affects boundary conditions of Droplet evaporation regime and transfer processes interaction in it was based on.
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Modelling of water Droplets heat and mass transfer in the course of Phase transitions. I. Phase transitions cycle peculiarities and iterative scheme of numerical research control and optimization
Nonlinear Analysis-Modelling and Control, 2016Co-Authors: Gintautas Miliauskas, Arvydas Adomavičius, Monika MaziukienėAbstract:The peculiarities of the widely applied in practice sprayed water Droplets Phase transition cycle are discussed in this article. Theoretical fundamentals of Droplets heat and mass transfer modelling by combined analytical-numerical method and numerical simulation peculiarities are outlined. Water Droplet Phase transitions were modelled on the energy flow balance condition basis. The control mechanism of iterative scheme used to determine the Droplet surface temperature was highlighted. The optimal finite number of members in the infinite integral equation set for Droplet temperature field and its gradient parameter calculation was defined by the numerical experiment.
R. Kranold - One of the best experts on this subject based on the ideXlab platform.
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Effect of the Cl− content on the formation and dissolution of precipitates in a soda–lime–silica glass
Journal of Non-crystalline Solids, 2001Co-Authors: R. Kranold, M. Kammel, A. HoellAbstract:Abstract The glass with composition 13Na2O–11CaO–76SiO2 (mol%) undergoes subliquidus Phase separation via a binodal mechanism. Below the binodal temperature, Tb, the glass separates into two amorphous Phases, silica-enriched Droplets and a silica-poor matrix. Small-angle X-ray scattering (SAXS) is used to study the formation process of the Droplet Phase at 600 °C, as well as, the inverse process where the precipitates dissolve after an increase in temperature. A small amount of 0.47 mol% Cl− in the glass causes a drastic change of the kinetics and thermodynamics of the Phase separation process in comparison with the pure glass: the Phase separation is accelerated and the maximum volume fraction of the silica Droplet Phase is increased. The results of dissolution experiments demonstrate that due to the Cl− content Tb increases by about 60 K. So, the main effect of Cl− in the glass is interpreted as a shift of the miscibility gap to higher temperatures resulting from a higher thermodynamic driving force.
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Reversion of precipitates in Phase separated soda lime silica glass
Journal of Applied Crystallography, 2000Co-Authors: R. Kranold, A. Hoell, M. Kammel, U. Lembke, G. Von KrosigkAbstract:The glass with composition 13Na 2 O-11CaO-76SiO 2 (mol%) undergoes subliquidus Phase separation via a binodal mechanism. At temperatures below the binodal temperature, T b =978 K, the glass separates into two amorphous Phases, silica-enriched Droplets and a silica-poor matrix. Small-angle X-ray scattering was used to study the formation of the Droplet Phase at 923 K as well as the process of reversion where the precipitates dissolve after an increase in temperature. After a heat treatment of 48 h at 923 K the system is in the stage of coarsening. However, the equilibrium volume fraction of the Droplet Phase, W c =0.073, has not yet precipitated. The reversion of the Droplets was studied at T=983 K and T = 963 K. For T>T b , the precipitates dissolve completely by an interdiffusion process resulting in a decrease of the silica concentration of the Droplets, while the Phase boundary is preserved over long times. For T
N V Surovtsev - One of the best experts on this subject based on the ideXlab platform.
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lipid Droplet Phase transition in freezing cat embryos and oocytes probed by raman spectroscopy
Biophysical Journal, 2018Co-Authors: K A Okotrub, V I Mokrousova, Sergei Amstislavsky, N V SurovtsevAbstract:Abstract Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid Droplets. We apply Raman spectroscopy to investigate freezing of lipid Droplets inside cumulus-oocyte complexes, mature oocytes, and early embryos of a domestic cat. Raman spectroscopy allows one to characterize the degree of lipid unsaturation, the lipid Phase transition from the liquid-like disordered to solid-like ordered state, and the triglyceride polymorphic state. For all cells examined, the average degree of lipid unsaturation is estimated as ∼1.3 (with ±20% deviation) double bonds per acyl chain. The onset of the lipid Phase transition occurs in a temperature range from −10 to +4°C and does not depend on the cell type. Lipid Droplets in cumulus-oocyte complexes are found to undergo abrupt lipid crystallization shifted in temperature from the ordering of the lipid conformational state. In the case of mature oocytes and early embryos obtained in vitro, the lipid crystallization is broadened. In the frozen state, lipid Droplets inside cumulus-oocyte complexes have a higher content of triglyceride polymorphic β and β′ Phases than estimated for mature oocytes and early embryos. For the first time, to our knowledge, the temperature evolution of the Phase state of lipid Droplets is examined. Raman spectroscopy is proved to be a promising tool for in situ monitoring of the lipid Phase state in a single embryo/oocyte during its freezing.
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lipid Droplet Phase transition in freezing cat embryos and oocytes probed by raman spectroscopy
bioRxiv, 2018Co-Authors: K A Okotrub, V I Mokrousova, Sergei Amstislavsky, N V SurovtsevAbstract:Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One challenging limitation of this technology is the cell damage during freezing associated with the intracellular lipid Droplets. We exploit a Raman spectroscopy to investigate the freezing of cumulus-oocyte complexes, mature oocytes and early embryos of a domestic cat. All these cells are rich in lipids. The degree of lipid unsaturation, lipid Phase transition from liquid-like disordered to solid-like ordered state (main transition) and triglyceride polymorphic state are studied. For all cells examined, the average degree of lipid unsaturation is estimated about 1.3 (with ±20 % deviation) double bonds per acyl chain. The onset of the main lipid Phase transition occurs in a temperature range from −10 to +4 °C and does not depend significantly on the cell type. It is found that lipid Droplets in cumulus-oocyte complexes undergo an abrupt lipid crystallization, which not completely correlate with the ordering of lipid molecule acyl chains. In the case of mature oocytes and early embryos obtained in vitro from cumulus-oocyte complexes, the lipid Phase transition is broadened. In frozen state lipid Droplets inside the cumulus-oocyte complexes have higher content of triglyceride polymorphic β and β′ Phases (~66%) than it is estimated for the mature oocytes and the early embryos (~50%). For the first time, to our knowledge, temperature evolution of lipid Droplets Phase state is examined. Raman spectroscopy is proved as a prospective tool for in situ monitoring of lipid Phase state in single embryo/oocyte during freezing.
