The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Xiaoyue Chen - One of the best experts on this subject based on the ideXlab platform.
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An Attenuated Duck Plague Virus (DPV) Vaccine Induces both Systemic and Mucosal Immune Responses To Protect Ducks against Virulent DPV Infection
2016Co-Authors: Xiaoyue Chen, Anchun C ChengaAbstract:Duck Plague (DP) is a severe disease caused by DP virus (DPV). Control of the disease is recognized as one of the biggest chal-lenges in avian medicine. Vaccination is an efficient way to control DPV, and an attenuated vaccine is the main routine vaccine. The attenuated DPV vaccine strain CHa is a modified live vaccine, but the systemic andmucosal immune responses induced by this vaccine have been poorly understood. In this study, the immunogenicity and efficacy of the vaccine were evaluated after sub-cutaneous immunization of Ducks. CD4 and CD8 T cells were counted by flow cytometry, and humoral andmucosal Ig anti-bodies were analyzed by enzyme-linked immunosorbent assay (ELISA). The results showed that high levels of T cells and Ig anti-bodies were present postimmunization and that there were more CD4 T cells than CD8 T cells. Titers of humoral IgG were higher than those of humoral IgA. Local IgA was found in each sample, whereas local IgG was found only in the spleen, thymus, bursa of Fabricius, harderian gland, liver, bile, and lung. In a protection assay, the attenuated DPV vaccine completely protected Ducks against 1,000 50 % lethal doses (LD50) of the lethal DPV strain CHv via oral infection. These data suggest that this subcuta-neous vaccine elicits sufficient systemic andmucosal immune responses against lethal DPV challenge to be protective in Ducks. This study provides broad insights into understanding the immune responses to the attenuated DPV vaccine strain CHa through subcutaneous immunization in Ducks. Duck Plague (DP), also known as Duck viral enteritis, is aworldwide disease caused by Duck Plague virus (DPV), a virus of the Herpesviridae family. DPV induces an acute disease wit
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
2013Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue ChenAbstract:Background: Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. Results: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Viru
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induction of immune responses in Ducks with a dna vaccine encoding Duck Plague virus glycoprotein c
Virology Journal, 2011Co-Authors: Bei Lian, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Fei Liu, Qihui Luo, Xinfeng Han, Xiaoyue ChenAbstract:Background A DNA vaccine expressing glycoprotein C (gC) of Duck Plague virus (DPV) was evaluated for inducing immunity in Ducks. The plasmid encoding gC of DPV was administered via intramuscular (IM) injection and gene gun bombardment.
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Cloning, expression and characterization of gE protein of Duck Plague virus
Virology journal, 2010Co-Authors: Hua Chang, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Xiaoyue Chen, Fei Liu, Zhengli Chen, Qihui Luo, Yi ZhouAbstract:Background The gE protein of Duck Plague virus is the important membrane glycoprotein, its protein characterization has not been reported. In this study, we expressed and presented the characterization of the DPV gE product.
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
Virology journal, 2010Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Renyong Jia, Dekang Zhu, Jun Ying Xiang, Xiaoyue ChenAbstract:Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Virus (DHV), Riemerella Anatipestifer(R. A), Escherichia coli (E. coli) and Salmonella anatum (S. anatum). Only antisera against DPV yielded a specific and strong signal. In order to determine the sensitivity of the TK-ELISA, a panel of diluted sera was tested, and the minimum detection limit of 1:2560 (OD450 nm = 0.401) was obtained according to the endpoint cut-off (0.2438). The repeatability and reproducibility under the experimental conditions demonstrates a low variability (P > 0.05). The suspected sera samples (n = 30) were determined by TK-ELISA and the positive rate is 90% (27/30), and the TK-ELISA showed 83.33% (22+3/30) coincidence rate with the Serum Neutralization Test (SNT) and 90% (24+3/30) coincidence rate with the whole DPV virion based-ELISA (DPV-ELISA). When defining the dynamics of antibody response to attenuated live DPV vaccine, the maximum antibodies is reached after 4 weeks. The results suggest that the TK-ELISA provides high specificity, sensitivity, repeatability and reproducibility for detection of anti-DPV antibodies in Duck sera, and has the potential to be much simpler than DPV-ELISA and SNT for the sera epidemiological investigation.
An Chun Cheng - One of the best experts on this subject based on the ideXlab platform.
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Duck Plague virus Glycoprotein J is functional but slightly impaired in viral replication and cell-to-cell spread.
Scientific reports, 2018Co-Authors: Yu You, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Shun Chen, Qiao Yang, Liu Tian, Mafeng LiuAbstract:To analyse the function of the Duck Plague virus (DPV) glycoprotein J homologue (gJ), two different mutated viruses, a gJ deleted mutant ΔgJ and a gJR rescue mutant gJR with US5 restored were generated. All recombinant viruses were constructed by using two-step of RED recombination system implemented on the Duck Plague virus Chinese virulent strain (DPV CHv) genome cloned into a bacterial artificial chromosome. DPV-mutants were characterized on non-complementing DEF cells compared with parental virus. Viral replication kinetics of intracellular and extracellular viruses revealed that the ΔgJ virus produce a 10-fold reduction of viral titers than the gJR and parental virus, which especially the production of extracellular infectivity was affected. In addition, the ΔgJ virus produced viral plaques on DEF cells that was on average approximately 11% smaller than those produced by the gJR and parental viruses. Electron microscopy confirmed that although DPV CHv without gJ could efficiently carry out viral replication, virion assembly and envelopment within infected cells, the ΔgJ virus produced and accumulated high levels of anuclear particles in the nuclear and cytoplasm. These results show that the gJ slightly impaired in viral replication, virion assembly and cell-to-cell spread, and is not essential in virion envelopment.
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Role of Duck Plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate
Journal of Integrative Agriculture, 2017Co-Authors: Yan-chun Jing, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Kunfeng Sun, Shun Chen, Mafeng Liu, Qiao YangAbstract:Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the Duck Plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrap™ Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent Duck Plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during Duck Plague virus infection.
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Analysis of synonymous codon usage in the US5 gene of Duck Plague Virus
2016Co-Authors: Ming Shu Wang, An Chun ChengAbstract:Abstract. The Duck Plague Virus (DPV) US5 gene was identified by constructing the DPV genomic library, the synonymous codon usage in the US5 gene of DPV and 11 reference herpesviruses have been investigated by using the CodonW 1.4 program, CUSP (create a codon usage table) program and CHIPS (calculated ENC value) of EMBOSS (The European Molecular Biology Open Software Suite). The results reveals that the synonymous codons with A and T at the third codon positon have widely usage in the codon of US5 gene of DPV. G + C compositional constraint is the main factor that determines the codon usage bias in US5 gene. In addition, rare condons analysis showed that there are 75 rare condons (13.9%) in the ORF of the DPV US5 gene on lin
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
2013Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue ChenAbstract:Background: Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. Results: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Viru
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Bioinformatics Analysis of UL39 Gene from Duck Plague Virus
Advanced Materials Research, 2013Co-Authors: Guo Fu Lu, An Chun Cheng, Ming Shu WangAbstract:The structures and characteristics of the large subunit of ribonucleotide reductase (R1), encoded by UL39 gene from Duck Plague virus (DPV) were analyzed and predicted by using a series of free bioinformatics software packages and bioinformatics tools. The DPV UL39 gene has a base composition of 681 adenine (27.99%), 503 cytosine (20.67%), 593 guanine (24.37%) and 656 thymine (26.96%). The online analysis of the physico-chemical properties demonstrates that the protein has 40 potential phosphorylation sites and 4 N-glycosylation sites when the threshold of prediction score is above 0.5; without the signal peptide and the transmembrance region. The phylogenetic tree proved that DPV R1 protein had a close evolutionary relationship with the Mardivirus genus of the Alphaherpesviruses. In conclusion, all those results will provide some valuable information for the further research of UL39 gene.
Ming Shu Wang - One of the best experts on this subject based on the ideXlab platform.
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Duck Plague virus Glycoprotein J is functional but slightly impaired in viral replication and cell-to-cell spread.
Scientific reports, 2018Co-Authors: Yu You, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Shun Chen, Qiao Yang, Liu Tian, Mafeng LiuAbstract:To analyse the function of the Duck Plague virus (DPV) glycoprotein J homologue (gJ), two different mutated viruses, a gJ deleted mutant ΔgJ and a gJR rescue mutant gJR with US5 restored were generated. All recombinant viruses were constructed by using two-step of RED recombination system implemented on the Duck Plague virus Chinese virulent strain (DPV CHv) genome cloned into a bacterial artificial chromosome. DPV-mutants were characterized on non-complementing DEF cells compared with parental virus. Viral replication kinetics of intracellular and extracellular viruses revealed that the ΔgJ virus produce a 10-fold reduction of viral titers than the gJR and parental virus, which especially the production of extracellular infectivity was affected. In addition, the ΔgJ virus produced viral plaques on DEF cells that was on average approximately 11% smaller than those produced by the gJR and parental viruses. Electron microscopy confirmed that although DPV CHv without gJ could efficiently carry out viral replication, virion assembly and envelopment within infected cells, the ΔgJ virus produced and accumulated high levels of anuclear particles in the nuclear and cytoplasm. These results show that the gJ slightly impaired in viral replication, virion assembly and cell-to-cell spread, and is not essential in virion envelopment.
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Role of Duck Plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate
Journal of Integrative Agriculture, 2017Co-Authors: Yan-chun Jing, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Kunfeng Sun, Shun Chen, Mafeng Liu, Qiao YangAbstract:Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the Duck Plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrap™ Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent Duck Plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during Duck Plague virus infection.
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Analysis of synonymous codon usage in the US5 gene of Duck Plague Virus
2016Co-Authors: Ming Shu Wang, An Chun ChengAbstract:Abstract. The Duck Plague Virus (DPV) US5 gene was identified by constructing the DPV genomic library, the synonymous codon usage in the US5 gene of DPV and 11 reference herpesviruses have been investigated by using the CodonW 1.4 program, CUSP (create a codon usage table) program and CHIPS (calculated ENC value) of EMBOSS (The European Molecular Biology Open Software Suite). The results reveals that the synonymous codons with A and T at the third codon positon have widely usage in the codon of US5 gene of DPV. G + C compositional constraint is the main factor that determines the codon usage bias in US5 gene. In addition, rare condons analysis showed that there are 75 rare condons (13.9%) in the ORF of the DPV US5 gene on lin
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
2013Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue ChenAbstract:Background: Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. Results: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Viru
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Bioinformatics Analysis of UL39 Gene from Duck Plague Virus
Advanced Materials Research, 2013Co-Authors: Guo Fu Lu, An Chun Cheng, Ming Shu WangAbstract:The structures and characteristics of the large subunit of ribonucleotide reductase (R1), encoded by UL39 gene from Duck Plague virus (DPV) were analyzed and predicted by using a series of free bioinformatics software packages and bioinformatics tools. The DPV UL39 gene has a base composition of 681 adenine (27.99%), 503 cytosine (20.67%), 593 guanine (24.37%) and 656 thymine (26.96%). The online analysis of the physico-chemical properties demonstrates that the protein has 40 potential phosphorylation sites and 4 N-glycosylation sites when the threshold of prediction score is above 0.5; without the signal peptide and the transmembrance region. The phylogenetic tree proved that DPV R1 protein had a close evolutionary relationship with the Mardivirus genus of the Alphaherpesviruses. In conclusion, all those results will provide some valuable information for the further research of UL39 gene.
Renyong Jia - One of the best experts on this subject based on the ideXlab platform.
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Duck Plague virus Glycoprotein J is functional but slightly impaired in viral replication and cell-to-cell spread.
Scientific reports, 2018Co-Authors: Yu You, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Shun Chen, Qiao Yang, Liu Tian, Mafeng LiuAbstract:To analyse the function of the Duck Plague virus (DPV) glycoprotein J homologue (gJ), two different mutated viruses, a gJ deleted mutant ΔgJ and a gJR rescue mutant gJR with US5 restored were generated. All recombinant viruses were constructed by using two-step of RED recombination system implemented on the Duck Plague virus Chinese virulent strain (DPV CHv) genome cloned into a bacterial artificial chromosome. DPV-mutants were characterized on non-complementing DEF cells compared with parental virus. Viral replication kinetics of intracellular and extracellular viruses revealed that the ΔgJ virus produce a 10-fold reduction of viral titers than the gJR and parental virus, which especially the production of extracellular infectivity was affected. In addition, the ΔgJ virus produced viral plaques on DEF cells that was on average approximately 11% smaller than those produced by the gJR and parental viruses. Electron microscopy confirmed that although DPV CHv without gJ could efficiently carry out viral replication, virion assembly and envelopment within infected cells, the ΔgJ virus produced and accumulated high levels of anuclear particles in the nuclear and cytoplasm. These results show that the gJ slightly impaired in viral replication, virion assembly and cell-to-cell spread, and is not essential in virion envelopment.
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Role of Duck Plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate
Journal of Integrative Agriculture, 2017Co-Authors: Yan-chun Jing, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Kunfeng Sun, Shun Chen, Mafeng Liu, Qiao YangAbstract:Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the Duck Plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrap™ Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent Duck Plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during Duck Plague virus infection.
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
2013Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue ChenAbstract:Background: Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. Results: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Viru
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induction of immune responses in Ducks with a dna vaccine encoding Duck Plague virus glycoprotein c
Virology Journal, 2011Co-Authors: Bei Lian, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Fei Liu, Qihui Luo, Xinfeng Han, Xiaoyue ChenAbstract:Background A DNA vaccine expressing glycoprotein C (gC) of Duck Plague virus (DPV) was evaluated for inducing immunity in Ducks. The plasmid encoding gC of DPV was administered via intramuscular (IM) injection and gene gun bombardment.
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Cloning, expression and characterization of gE protein of Duck Plague virus
Virology journal, 2010Co-Authors: Hua Chang, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Xiaoyue Chen, Fei Liu, Zhengli Chen, Qihui Luo, Yi ZhouAbstract:Background The gE protein of Duck Plague virus is the important membrane glycoprotein, its protein characterization has not been reported. In this study, we expressed and presented the characterization of the DPV gE product.
Dekang Zhu - One of the best experts on this subject based on the ideXlab platform.
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Duck Plague virus Glycoprotein J is functional but slightly impaired in viral replication and cell-to-cell spread.
Scientific reports, 2018Co-Authors: Yu You, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Shun Chen, Qiao Yang, Liu Tian, Mafeng LiuAbstract:To analyse the function of the Duck Plague virus (DPV) glycoprotein J homologue (gJ), two different mutated viruses, a gJ deleted mutant ΔgJ and a gJR rescue mutant gJR with US5 restored were generated. All recombinant viruses were constructed by using two-step of RED recombination system implemented on the Duck Plague virus Chinese virulent strain (DPV CHv) genome cloned into a bacterial artificial chromosome. DPV-mutants were characterized on non-complementing DEF cells compared with parental virus. Viral replication kinetics of intracellular and extracellular viruses revealed that the ΔgJ virus produce a 10-fold reduction of viral titers than the gJR and parental virus, which especially the production of extracellular infectivity was affected. In addition, the ΔgJ virus produced viral plaques on DEF cells that was on average approximately 11% smaller than those produced by the gJR and parental viruses. Electron microscopy confirmed that although DPV CHv without gJ could efficiently carry out viral replication, virion assembly and envelopment within infected cells, the ΔgJ virus produced and accumulated high levels of anuclear particles in the nuclear and cytoplasm. These results show that the gJ slightly impaired in viral replication, virion assembly and cell-to-cell spread, and is not essential in virion envelopment.
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Role of Duck Plague virus glycoprotein C in viral adsorption: Absence of specific interactions with cell surface heparan sulfate
Journal of Integrative Agriculture, 2017Co-Authors: Yan-chun Jing, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Kunfeng Sun, Shun Chen, Mafeng Liu, Qiao YangAbstract:Many mammalian herpes viruses utilize heparan sulfate (HS) moieties present on cell surface proteoglycans as receptors for cell entry, and this process also requires viral glycoprotein C (gC) homologues. However, our understanding of the role of gC in facilitating attachment of other alpha-herpes viruses such as the Duck Plague virus (DPV) remains preliminary. To study the role of gC during DPV infection, we used a gC-deleted mutant virus (DPV-ΔgC-EGFP). Examination of the viral copy number by real-time PCR, as well as time course studies of viral adsorption and proliferation revealed that gC was involved in the viral binding to the cell surface. The affinity of viral glycoproteins (gB-DPV, gC-DPV, and gE-DPV) to HS was assessed using a prokaryotic expression system and HiTrap™ Heparin HP column chromatography. In addition, to confirm that gC played a role in the interaction between DPV and HS, viruses were treated with the HS analogue heparin and host cells were treated with its inhibitors heparinase prior to exposure to DPV-ΔgC-EGFP or wild-type strain Chinese virulent Duck Plague virus (DPV-CHv). The effects of heparin and heparinase on virus infectivity demonstrated that function of gC on viral adsorption is independent of interactions between gC and heparin sulfate on cell surface. All in all, this study demonstrated that the gC of DPV can mediate viral adsorption in an HS-independent manner, which distinguish it from the gC of some other alpha-herpes viruses. Future studies will be required to identify the receptors involved in gC protein binding to cells. This work provides us a foundation for further studies of examining the roles of gC in the adsorption during Duck Plague virus infection.
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A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus
2013Co-Authors: Yongping Wen, An Chun Cheng, Ming Shu Wang, Chanjuan Shen, Sitong Liu, Jun Xiang, Renyong Jia, Dekang Zhu, Xiaoyue ChenAbstract:Background: Duck Plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout Duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology. Results: In this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Viru
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induction of immune responses in Ducks with a dna vaccine encoding Duck Plague virus glycoprotein c
Virology Journal, 2011Co-Authors: Bei Lian, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Fei Liu, Qihui Luo, Xinfeng Han, Xiaoyue ChenAbstract:Background A DNA vaccine expressing glycoprotein C (gC) of Duck Plague virus (DPV) was evaluated for inducing immunity in Ducks. The plasmid encoding gC of DPV was administered via intramuscular (IM) injection and gene gun bombardment.
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Cloning, expression and characterization of gE protein of Duck Plague virus
Virology journal, 2010Co-Authors: Hua Chang, An Chun Cheng, Ming Shu Wang, Renyong Jia, Dekang Zhu, Xiaoyue Chen, Fei Liu, Zhengli Chen, Qihui Luo, Yi ZhouAbstract:Background The gE protein of Duck Plague virus is the important membrane glycoprotein, its protein characterization has not been reported. In this study, we expressed and presented the characterization of the DPV gE product.
