The Experts below are selected from a list of 5532 Experts worldwide ranked by ideXlab platform
Xiaoying Wang - One of the best experts on this subject based on the ideXlab platform.
-
quaternized chitosan rectorite intercalative materials for a gene delivery system
Nanotechnology, 2008Co-Authors: Yumin Du, Xiaoying Wang, Yan LiAbstract:Non-viral vectors have gained increasing attention in gene therapy because of their safety, but with the shortcoming of low transfection efficiency. We have developed a hybrid material as a novel non-viral vector, which combines the advantages of both biopolymer and clay in a gene delivery system. Quaternized chitosan was intercalated into the interlayers of rectorite to obtain a new polymer/layered silicate nanocomposite. In vitro and in vivo toxicity studies revealed that the nanocomposites were biocompatible and non-toxic. At the nanocomposite:pDNA mass ratio of 8:1, they achieved 100% pDNA adsorption capacity. In vitro cell transfection revealed a transfection efficiency of 32.1% at 96 h as shown by a flow-cytometric study, and the intensive green fluorescence protein (GFP) expression could last for up to 120 h. Furthermore, an in vivo transfection study showed that the most prominent GFP expression was observed in the gastric and Duodenum Mucosa, and good transfection efficiency was also obtained when injected into the muscle. All the results suggest that quaternized chitosan/rectorite nanocomposite is a novel and potential non-viral gene carrier.
Deyan Zhao - One of the best experts on this subject based on the ideXlab platform.
-
Table_1_Functional Prediction of Chronic Kidney Disease Susceptibility Gene PRKAG2 by Comprehensively Bioinformatics Analysis.DOC
2018Co-Authors: Ermin Wang, Hainan Zhao, Deyan ZhaoAbstract:The genetic predisposition to chronic kidney disease (CKD) has been widely evaluated especially using the genome-wide association studies, which highlighted some novel genetic susceptibility variants in many genes, and estimated glomerular filtration rate to diagnose and stage CKD. Of these variants, rs7805747 in PRKAG2 was identified to be significantly associated with both serum creatinine and CKD with genome wide significance level. Until now, the potential mechanism by which rs7805747 affects CKD risk is still unclear. Here, we performed a functional analysis of rs7805747 variant using multiple bioinformatics software and databases. Using RegulomeDB and HaploReg (version 4.1), rs7805747 was predicated to locate in enhancer histone marks (Liver, Duodenum Mucosa, Fetal Intestine Large, Fetal Intestine Small, and Right Ventricle tissues). Using GWAS analysis in PhenoScanner, we showed that rs7805747 is not only associated with CKD, but also is significantly associated with other diseases or phenotypes. Using metabolite analysis in PhenoScanner, rs7805747 is identified to be significantly associated with not only the serum creatinine, but also with other 16 metabolites. Using eQTL analysis in PhenoScanner, rs7805747 is identified to be significantly associated with gene expression in multiple human tissues and multiple genes including PRKAG2. The gene expression analysis of PRKAG2 using 53 tissues from GTEx RNA-Seq of 8555 samples (570 donors) in GTEx showed that PRKAG2 had the highest median expression in Heart-Atrial Appendage. Using the gene expression profiles in human CKD, we further identified different expression of PRKAG2 gene in CKD cases compared with control samples. In summary, our findings provide new insight into the underlying susceptibility of PRKAG2 gene to CKD.
-
Functional Prediction of Chronic Kidney Disease Susceptibility Gene PRKAG2 by Comprehensively Bioinformatics Analysis
Frontiers Media S.A., 2018Co-Authors: Ermin Wang, Hainan Zhao, Deyan ZhaoAbstract:The genetic predisposition to chronic kidney disease (CKD) has been widely evaluated especially using the genome-wide association studies, which highlighted some novel genetic susceptibility variants in many genes, and estimated glomerular filtration rate to diagnose and stage CKD. Of these variants, rs7805747 in PRKAG2 was identified to be significantly associated with both serum creatinine and CKD with genome wide significance level. Until now, the potential mechanism by which rs7805747 affects CKD risk is still unclear. Here, we performed a functional analysis of rs7805747 variant using multiple bioinformatics software and databases. Using RegulomeDB and HaploReg (version 4.1), rs7805747 was predicated to locate in enhancer histone marks (Liver, Duodenum Mucosa, Fetal Intestine Large, Fetal Intestine Small, and Right Ventricle tissues). Using GWAS analysis in PhenoScanner, we showed that rs7805747 is not only associated with CKD, but also is significantly associated with other diseases or phenotypes. Using metabolite analysis in PhenoScanner, rs7805747 is identified to be significantly associated with not only the serum creatinine, but also with other 16 metabolites. Using eQTL analysis in PhenoScanner, rs7805747 is identified to be significantly associated with gene expression in multiple human tissues and multiple genes including PRKAG2. The gene expression analysis of PRKAG2 using 53 tissues from GTEx RNA-Seq of 8555 samples (570 donors) in GTEx showed that PRKAG2 had the highest median expression in Heart-Atrial Appendage. Using the gene expression profiles in human CKD, we further identified different expression of PRKAG2 gene in CKD cases compared with control samples. In summary, our findings provide new insight into the underlying susceptibility of PRKAG2 gene to CKD
Miguel Perezmateo - One of the best experts on this subject based on the ideXlab platform.
-
ultrastructural characteristics of distal Duodenum Mucosa in patients with cirrhosis
European Journal of Gastroenterology & Hepatology, 2002Co-Authors: Jose Such, Jose V Guardiola, Joaquin De Juan, J A Casellas, Sonia Pascual, Jose Ramon Aparicio, Javier Solavera, Miguel PerezmateoAbstract:Objectives Morphological abnormalities observed by light microscopy, such as oedema or vasodilatation, have been described in intestinal Mucosa of patients with cirrhosis, but no information is available regarding the ultrastructural characteristics of the intestinal epithelial layer. The aim of this observational study was to investigate the ultrastructural characteristics of the intestinal epithelial layer of Duodenum Mucosa in patients with cirrhosis. Methods Six patients with advanced cirrhosis and six control patients without liver disease were included in the study. Biopsies were obtained from the distal Duodenum during upper diagnostic endoscopy, and ultrastructural characteristics were studied by means of electron microscopy. Results A distended interenterocyte space with intestinal epithelial cells closely attached by morphologically intact tight junctions has been observed in cirrhotic patients, together with shorter and wider microvilli than in the control subjects. Conclusions The epithelial layers from cirrhotic patients show ultrastructural abnormalities. However, it is not known if the observed alterations are related to the presence of increased intestinal permeability or to bacterial translocation, which are frequently found in these patients.
Yan Li - One of the best experts on this subject based on the ideXlab platform.
-
quaternized chitosan rectorite intercalative materials for a gene delivery system
Nanotechnology, 2008Co-Authors: Yumin Du, Xiaoying Wang, Yan LiAbstract:Non-viral vectors have gained increasing attention in gene therapy because of their safety, but with the shortcoming of low transfection efficiency. We have developed a hybrid material as a novel non-viral vector, which combines the advantages of both biopolymer and clay in a gene delivery system. Quaternized chitosan was intercalated into the interlayers of rectorite to obtain a new polymer/layered silicate nanocomposite. In vitro and in vivo toxicity studies revealed that the nanocomposites were biocompatible and non-toxic. At the nanocomposite:pDNA mass ratio of 8:1, they achieved 100% pDNA adsorption capacity. In vitro cell transfection revealed a transfection efficiency of 32.1% at 96 h as shown by a flow-cytometric study, and the intensive green fluorescence protein (GFP) expression could last for up to 120 h. Furthermore, an in vivo transfection study showed that the most prominent GFP expression was observed in the gastric and Duodenum Mucosa, and good transfection efficiency was also obtained when injected into the muscle. All the results suggest that quaternized chitosan/rectorite nanocomposite is a novel and potential non-viral gene carrier.
Xiao Chuanbin - One of the best experts on this subject based on the ideXlab platform.
-
immunological enhancement effects of il 2 on the Duodenum Mucosa in gushi chicken
Chinese journal of veterinary science, 2009Co-Authors: Zhang Shusong, Liu Zhonghu, Kang Xiangtao, Zhao Feng, Xiao ChuanbinAbstract:To investigate the immunological enhancement effects of IL-2 with different doses in chicken Shigella vaccine on the Duodenum Mucosa in Gushi chicken,parental line Gushi chicken were grouped and inoculated with Shigella vaccines including different dose of IL-2.And then the distribution and change of SIgA positive cells and its secretion in the Duodenum of the chicken were observed with the immunohistochemistry technology using the Qwin image analysis system.SIgA postive cells were distributed and located primarily in inhesion membrane and enteraden cavity of the Duodenum Mucosa.additive IL-2 in the Shigella vaccine could increase secretion of SIgA positive cells and strengthen the immunity of the birds.in some degree.The 50 μg IL-2 in the vaccine could display a optimal immunological enhancement effect.
