The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Michael Vollrath - One of the best experts on this subject based on the ideXlab platform.
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The lymphocyte transformation test with type II collagen as a diagnostic tool of autoimmune sensorineural hEaring loss.
The Laryngoscope, 1991Co-Authors: Peter Berger, Matthias Hillman, Mehmet Tabak, Michael VollrathAbstract:Immunological disorders of the cellular type can be diagnosed by the lymphocyte transformation test (LTT). An autoimmune mechanism of certain cases of sensorineural hEaring loss (SNHL) can be evaluated by using human inner Ear Tissue as an antigen. Recent studies have shown that type II collagen plays an important role, not only in some autoimmune mediated rheumatoid diseases, but also as an antigenic substrate of inner Ear Tissue in autoimmune sensorineural hEaring loss. This paper deals with results of the lymphocyte transformation test using type II collagen as antigen in 68 patients with progressive sensorineural hEaring loss (PSNHL) and 68 healthy volunteers. Thirty-four patients showed a strong stimulation in the lymphocyte transformation test, in contrast to only four volunteers in the control group, two of whom had a history of rheumatoid arthritis.
A. Ballagi - One of the best experts on this subject based on the ideXlab platform.
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detection of bovine viral diarrhoea virus infected cattle testing Tissue samples derived from Ear tagging using an erns capture elisa
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2005Co-Authors: S. Kuhne, C. Schroeder, G. Holmquist, G. Wolf, S. Horner, Gottfried Brem, A. BallagiAbstract:Summary A new diagnostic approach testing Tissue samples derived from cattle Ear tagging for bovine viral diarrhoea virus (BVDV) antigen in a commercially available antigen capture enzymelinked immunosorbent assay (ACE) was developed. To validate this method, 99 positive and 469 negative samples were tested. With those samples the assay yielded a sensitivity of 100% and specificity of ‡99.6%. Serum and Ear Tissue samples from 11 persistently infected (PI) BVDV calves were tested. While serum samples were negative after intake of colostrum, the Ear Tissue samples could be detected positive for BVDV all the time. Testing multiple samples derived from the same Ear from PI cattle yielded positive results and low variation. Using cattle Ear tags combining the Ear tag application with sampling of a small Ear Tissue plug and testing those Tissue samples with an ACE could be a reliable and economic way of BVDV testing.
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Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an Erns Capture ELISA
Journal of veterinary medicine. B Infectious diseases and veterinary public health, 2005Co-Authors: S. Kuhne, C. Schroeder, G. Holmquist, G. Wolf, S. Horner, Gottfried Brem, A. BallagiAbstract:Summary A new diagnostic approach testing Tissue samples derived from cattle Ear tagging for bovine viral diarrhoea virus (BVDV) antigen in a commercially available antigen capture enzymelinked immunosorbent assay (ACE) was developed. To validate this method, 99 positive and 469 negative samples were tested. With those samples the assay yielded a sensitivity of 100% and specificity of ‡99.6%. Serum and Ear Tissue samples from 11 persistently infected (PI) BVDV calves were tested. While serum samples were negative after intake of colostrum, the Ear Tissue samples could be detected positive for BVDV all the time. Testing multiple samples derived from the same Ear from PI cattle yielded positive results and low variation. Using cattle Ear tags combining the Ear tag application with sampling of a small Ear Tissue plug and testing those Tissue samples with an ACE could be a reliable and economic way of BVDV testing.
Peter Berger - One of the best experts on this subject based on the ideXlab platform.
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The lymphocyte transformation test with type II collagen as a diagnostic tool of autoimmune sensorineural hEaring loss.
The Laryngoscope, 1991Co-Authors: Peter Berger, Matthias Hillman, Mehmet Tabak, Michael VollrathAbstract:Immunological disorders of the cellular type can be diagnosed by the lymphocyte transformation test (LTT). An autoimmune mechanism of certain cases of sensorineural hEaring loss (SNHL) can be evaluated by using human inner Ear Tissue as an antigen. Recent studies have shown that type II collagen plays an important role, not only in some autoimmune mediated rheumatoid diseases, but also as an antigenic substrate of inner Ear Tissue in autoimmune sensorineural hEaring loss. This paper deals with results of the lymphocyte transformation test using type II collagen as antigen in 68 patients with progressive sensorineural hEaring loss (PSNHL) and 68 healthy volunteers. Thirty-four patients showed a strong stimulation in the lymphocyte transformation test, in contrast to only four volunteers in the control group, two of whom had a history of rheumatoid arthritis.
Tatsuya Yamasoba - One of the best experts on this subject based on the ideXlab platform.
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Expression of ACE2, TMPRSS2, and Furin in Mouse Ear Tissue, and the Implications for SARS-CoV-2 Infection.
The Laryngoscope, 2020Co-Authors: Tsukasa Uranaka, Akinori Kashio, Rumi Ueha, Taku J. Sato, Makoto Kinoshita, Kenji Kondo, Han Bing, Gao Ying, Tatsuya YamasobaAbstract:Objectives/hypothesis Intracellular entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) depends on the interaction between its spike protein with the cellular receptor angiotensin-converting enzyme 2 (ACE2) and depends on Furin-mediated spike protein cleavage and spike protein priming by host cell proteases, including transmembrane protease serine 2 (TMPRSS2). As the expression of ACE2, TMPRSS2, and Furin in the middle and inner Ear remain unclEar, we analyzed the expression of these proteins in mouse Ear Tissues. Study design Animal ResEarch. Methods We performed immunohistochemical analysis to examine the distribution of ACE2, TMPRSS2, and Furin in the Eustachian tube, middle Ear spaces, and cochlea of mice. Results ACE2 was present in the nucleus of the epithelium of the middle Ear and Eustachian tube, as well as in some nuclei of the hair cells in the organ of Corti, in the stria vascularis, and the spiral ganglion cells. ACE2 was also expressed in the cytoplasm of the stria vascularis. TMPRSS2 was expressed in both the nucleus and cytoplasm in the middle spaces, with the expression being stronger in the nucleus in the mucosal epithelium of the middle Ear spaces and Eustachian tube. TMPRSS2 was present in the cytoplasm in the organ of Corti and stria vascularis and in the nucleus and cytoplasm in the spiral ganglion. Furin was expressed in the cytoplasm in the middle Ear spaces, Eustachian tube, and cochlea. Conclusions ACE2, TMPRSS2, and Furin are diffusely present in the Eustachian tube, middle Ear spaces, and cochlea, suggesting that these Tissues are susceptible to SARS-CoV-2 infection. Level of evidence NA Laryngoscope, 2020.
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Expression of Ace2, Tmprss2, and Furin in mouse Ear Tissue
2020Co-Authors: Tsukasa Uranaka, Akinori Kashio, Rumi Ueha, Taku J. Sato, Bing Han, Ying Gao, Makoto Kinoshita, Kenji Kondo, Tatsuya YamasobaAbstract:Objectives: Intracellular entry of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) depends on the interaction between its spike protein to a cellular receptor named angiotensin-converting enzyme 2 (ACE2) and depends on Furin-mediated spike 23 protein cleavage and spike protein priming by host cell proteases including 24 transmembrane protease serine 2 (TMPRSS2). Tmprss1, Tmprss3, and Tmprss5 are expressed in the spiral ganglion neurons and the organ of Corti in the inner Ear; however, Ace2, Tmprss2, and Furin expression profiles in the middle Ear remain unclEar. Therefore, this study aimed to analyze Ace2, Tmprss2, and Furin expression in the middle and inner Ear of mice. Study Design: Animal resEarch. Setting: Department of Otolaryngology and Head and Neck Surgery, University of Tokyo. Methods: We performed immunohistochemical analysis to examine the distribution of Ace2, Tmprss2, and Furin in the eustachian tube, middle Ear space, and cochlea of mice. Results: Ace2 was expressed in the cytoplasm in the middle Ear epithelium, eustachian tube epithelium, stria vascularis, and spiral ganglion. Tmprss2 and Furin were widely expressed in the middle Ear spaces and the cochlea. Conclusion: Co-expression of Ace2, Tmprss2, and Furin in the middle Ear indicates that the middle Ear is susceptible to SARS-CoV-2 infections, thus warranting the use of personal protective equipment during mastoidectomy for coronavirus disease (COVID-19) patients.
S. Kuhne - One of the best experts on this subject based on the ideXlab platform.
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detection of bovine viral diarrhoea virus infected cattle testing Tissue samples derived from Ear tagging using an erns capture elisa
Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health, 2005Co-Authors: S. Kuhne, C. Schroeder, G. Holmquist, G. Wolf, S. Horner, Gottfried Brem, A. BallagiAbstract:Summary A new diagnostic approach testing Tissue samples derived from cattle Ear tagging for bovine viral diarrhoea virus (BVDV) antigen in a commercially available antigen capture enzymelinked immunosorbent assay (ACE) was developed. To validate this method, 99 positive and 469 negative samples were tested. With those samples the assay yielded a sensitivity of 100% and specificity of ‡99.6%. Serum and Ear Tissue samples from 11 persistently infected (PI) BVDV calves were tested. While serum samples were negative after intake of colostrum, the Ear Tissue samples could be detected positive for BVDV all the time. Testing multiple samples derived from the same Ear from PI cattle yielded positive results and low variation. Using cattle Ear tags combining the Ear tag application with sampling of a small Ear Tissue plug and testing those Tissue samples with an ACE could be a reliable and economic way of BVDV testing.
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Detection of Bovine Viral Diarrhoea Virus Infected Cattle – Testing Tissue Samples Derived from Ear Tagging Using an Erns Capture ELISA
Journal of veterinary medicine. B Infectious diseases and veterinary public health, 2005Co-Authors: S. Kuhne, C. Schroeder, G. Holmquist, G. Wolf, S. Horner, Gottfried Brem, A. BallagiAbstract:Summary A new diagnostic approach testing Tissue samples derived from cattle Ear tagging for bovine viral diarrhoea virus (BVDV) antigen in a commercially available antigen capture enzymelinked immunosorbent assay (ACE) was developed. To validate this method, 99 positive and 469 negative samples were tested. With those samples the assay yielded a sensitivity of 100% and specificity of ‡99.6%. Serum and Ear Tissue samples from 11 persistently infected (PI) BVDV calves were tested. While serum samples were negative after intake of colostrum, the Ear Tissue samples could be detected positive for BVDV all the time. Testing multiple samples derived from the same Ear from PI cattle yielded positive results and low variation. Using cattle Ear tags combining the Ear tag application with sampling of a small Ear Tissue plug and testing those Tissue samples with an ACE could be a reliable and economic way of BVDV testing.
