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Yuswanto Agustinus - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PEMBERIAN EKSTRAK ZAT PEDAS RIMPANG JAHE EMPRIT YANG DISARI DENGAN ETANOL 70% TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Mellawati Dyah, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Immunomodulator has been used as one of the attention to develop herbal medicine. Jahe is one of herbal medicine which is usually used to heal various diseases. Jahe Emprit is one of local name of Jahe in Indonesia. The aim of this research was to investigate the effect of pungent principle containing Extract of Jahe emprit rhizome on the macrophage activity compared by levamisol and Echinacea Extract. Extraction was done by 70%v/v ethanol. The concentrations of Extract were 5 mg/kg B.W; 25 mg/kg B.W; and 100 mg/kg B.W. CMC Na 1,5%w/v was used as solvent control. Levamisol 2,5 mg/kg B.W and Echinacea Extract 10 mg/kg B.W were used as positive control. The macrophage ability were measured according to Leijh et al., (1986) that was basically on the amount of latex which was captured by macrophage. The Extracts were orally administrated to the Swiss male mice at the dose of 0,2 ml/20 g B.W. The Macrophage activity was analyzed by one way ANOVA at 95% of confidence level. The Extract which had specification of 35% relative concentration of pungent principles; 3,554% ± 0,145 % W/W total phenolic substances; 13,70 mg/ml antioxydative value (IC50) at the concentrations of 5 mg/kg B.W and 25 mg/kg B.W could increase phagocytosis ability. The ability of the Extract at the concentration 25 mg/kg.B.W had no different with either the Levamizol 2,5 mg/kg. B.W., and Echinacea Extract 2,5 mg/kg. B.W.Efek immunomodulator banyak dilakukan dalam upaya pengembangan herbal medisin. Jahe merupakan salah satu bahan alami yang digunakan untuk mengatasi berbagai macam penyakit. Jahe emprit merupakan salah satu nama lokal jahe yang ada di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak zat pedas rimpang jahe emprit yang diekstraksi dengan 70% v/v etanol terhadap aktivitas makrofag, dibandingkan dengan levamisol dan ekstrak Echinacea. Ekstraksi dilakukan dengan etanol 70% v/v. Kemampuan fagositosis makrofag terhadap lateks ekstrak zat pedas rimpang jahe emprit secara in vitro pada 5 mg/kgBB; 25 mg/kgBB; dan 100 mg/kgBB. CMC Na 1,5% b/v digunakan sebagai kontrol pelarut; sedangkan sebagai kontrol positif, digunakan Levamisol 2,5 mg/kg BB dan ekstrak Ehinacea 10 mg/kg BB. Metode pengujian yang dilakukan, sesuai dengan pengujian yang dilakukan Leijh dkk (1986). Ekstrak diberikan secara oral pada mencit jantan galur Swiss sebanyak 0,2 ml/20 g BB. Data dianalisis dengan ANOVA satu jalan (taraf kepercayaan 95%). Ekstrak zat pedas rimpang jahe emprit dengan spesifikasi kadar relatif zat pedas 35%, kadar fenol total 3,554% ± 0,145 % b/b; dan bilangan antioksidan (IC50) 13,70 mg/ml, pada dosis 5 mg/kg BB dan 25 mg/kg BB dapat berefek pada peningkatan ke-mampuan fagositosis makrofag peritoneal pada mencit jantan yang diinfeksi Listeria monocytogenes. Peningkatan fagositosis ekstrak zat pedas rimpang jahe emprit dosis 25 mg/kg BB sebanding dengan imunostimulator sintetik (Levamisol hidroklorida 2,5 mg/kg BB) dan imunostimulator alami (ekstrak Echinacea 10mg/ kgBB)
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PENGARUH PEMBERIAN EKSTRAK ETANOLIK RIMPANG JAHE MERAH TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Ys Bintari, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle Extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle Extract of “jahe merah” rhizome ethanolic Extract, that Extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle Extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic Extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic Extract of jahe merah was the same with Echinacea Extract and Levamisol. Jahe merupakan salah satu tanaman obat yang digunakan pada kondisi individu yang berada pada kondisi tidak nyaman; misalnya sakit kepala, diare, kembung, demam, batuk, dan masuk angin. Tujuan penelitian ini untuk mengetahui efek ekstrak zat pedas rimpang jahe merah dalam kaitannya dengan peningkatan aktivitas fagositosis makrofag. Metode penelitian dilakukan sesuai dengan metode yang pernah dilakukan oleh Leijh dkk. (1986) yaitu dengan lateks beads. Efek ekstrak zat pedas rimpang jahe merah 10 mg/kgBB, 25 mg/ kgBB, dan 100 mg/kgBB dilihat terhadap jumlah lateks yang difagositosis makrofag. Perlakuan dilakukan selama 20 hari. Pada hari ke-15, mencit diinfeksi dengan Listeria monocytogenes. Data dianalisis dengan Kolmogorov-Smirnov, dan ANOVA satu arah dengan taraf kepercayaan 95%. Hasil penelitian disimpulkan bahwa ekstrak zat pedas rimpang jahe merah 100 mg/kgBB, dengan perbandingan kadar relatif bercak zat pedas 1 dan bercak zat pedas 2 sebesar 6,8:1, kadar fenolik total 3,27% b/b EAG (Ekuivalen Asam Galat), dan IC50 14,57 mg/mL, berefek pada peningkatan aktivitas makrofag. Peningkatan fagositosis makrofag ekstrak zat pedas rimpang jahe merah 100 mg/kgBB sebanding dengan ekstrak Echinacea dan levamisol
Sudarsono Sudarsono - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PEMBERIAN EKSTRAK ZAT PEDAS RIMPANG JAHE EMPRIT YANG DISARI DENGAN ETANOL 70% TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Mellawati Dyah, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Immunomodulator has been used as one of the attention to develop herbal medicine. Jahe is one of herbal medicine which is usually used to heal various diseases. Jahe Emprit is one of local name of Jahe in Indonesia. The aim of this research was to investigate the effect of pungent principle containing Extract of Jahe emprit rhizome on the macrophage activity compared by levamisol and Echinacea Extract. Extraction was done by 70%v/v ethanol. The concentrations of Extract were 5 mg/kg B.W; 25 mg/kg B.W; and 100 mg/kg B.W. CMC Na 1,5%w/v was used as solvent control. Levamisol 2,5 mg/kg B.W and Echinacea Extract 10 mg/kg B.W were used as positive control. The macrophage ability were measured according to Leijh et al., (1986) that was basically on the amount of latex which was captured by macrophage. The Extracts were orally administrated to the Swiss male mice at the dose of 0,2 ml/20 g B.W. The Macrophage activity was analyzed by one way ANOVA at 95% of confidence level. The Extract which had specification of 35% relative concentration of pungent principles; 3,554% ± 0,145 % W/W total phenolic substances; 13,70 mg/ml antioxydative value (IC50) at the concentrations of 5 mg/kg B.W and 25 mg/kg B.W could increase phagocytosis ability. The ability of the Extract at the concentration 25 mg/kg.B.W had no different with either the Levamizol 2,5 mg/kg. B.W., and Echinacea Extract 2,5 mg/kg. B.W.Efek immunomodulator banyak dilakukan dalam upaya pengembangan herbal medisin. Jahe merupakan salah satu bahan alami yang digunakan untuk mengatasi berbagai macam penyakit. Jahe emprit merupakan salah satu nama lokal jahe yang ada di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak zat pedas rimpang jahe emprit yang diekstraksi dengan 70% v/v etanol terhadap aktivitas makrofag, dibandingkan dengan levamisol dan ekstrak Echinacea. Ekstraksi dilakukan dengan etanol 70% v/v. Kemampuan fagositosis makrofag terhadap lateks ekstrak zat pedas rimpang jahe emprit secara in vitro pada 5 mg/kgBB; 25 mg/kgBB; dan 100 mg/kgBB. CMC Na 1,5% b/v digunakan sebagai kontrol pelarut; sedangkan sebagai kontrol positif, digunakan Levamisol 2,5 mg/kg BB dan ekstrak Ehinacea 10 mg/kg BB. Metode pengujian yang dilakukan, sesuai dengan pengujian yang dilakukan Leijh dkk (1986). Ekstrak diberikan secara oral pada mencit jantan galur Swiss sebanyak 0,2 ml/20 g BB. Data dianalisis dengan ANOVA satu jalan (taraf kepercayaan 95%). Ekstrak zat pedas rimpang jahe emprit dengan spesifikasi kadar relatif zat pedas 35%, kadar fenol total 3,554% ± 0,145 % b/b; dan bilangan antioksidan (IC50) 13,70 mg/ml, pada dosis 5 mg/kg BB dan 25 mg/kg BB dapat berefek pada peningkatan ke-mampuan fagositosis makrofag peritoneal pada mencit jantan yang diinfeksi Listeria monocytogenes. Peningkatan fagositosis ekstrak zat pedas rimpang jahe emprit dosis 25 mg/kg BB sebanding dengan imunostimulator sintetik (Levamisol hidroklorida 2,5 mg/kg BB) dan imunostimulator alami (ekstrak Echinacea 10mg/ kgBB)
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PENGARUH PEMBERIAN EKSTRAK ETANOLIK RIMPANG JAHE MERAH TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Ys Bintari, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle Extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle Extract of “jahe merah” rhizome ethanolic Extract, that Extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle Extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic Extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic Extract of jahe merah was the same with Echinacea Extract and Levamisol. Jahe merupakan salah satu tanaman obat yang digunakan pada kondisi individu yang berada pada kondisi tidak nyaman; misalnya sakit kepala, diare, kembung, demam, batuk, dan masuk angin. Tujuan penelitian ini untuk mengetahui efek ekstrak zat pedas rimpang jahe merah dalam kaitannya dengan peningkatan aktivitas fagositosis makrofag. Metode penelitian dilakukan sesuai dengan metode yang pernah dilakukan oleh Leijh dkk. (1986) yaitu dengan lateks beads. Efek ekstrak zat pedas rimpang jahe merah 10 mg/kgBB, 25 mg/ kgBB, dan 100 mg/kgBB dilihat terhadap jumlah lateks yang difagositosis makrofag. Perlakuan dilakukan selama 20 hari. Pada hari ke-15, mencit diinfeksi dengan Listeria monocytogenes. Data dianalisis dengan Kolmogorov-Smirnov, dan ANOVA satu arah dengan taraf kepercayaan 95%. Hasil penelitian disimpulkan bahwa ekstrak zat pedas rimpang jahe merah 100 mg/kgBB, dengan perbandingan kadar relatif bercak zat pedas 1 dan bercak zat pedas 2 sebesar 6,8:1, kadar fenolik total 3,27% b/b EAG (Ekuivalen Asam Galat), dan IC50 14,57 mg/mL, berefek pada peningkatan aktivitas makrofag. Peningkatan fagositosis makrofag ekstrak zat pedas rimpang jahe merah 100 mg/kgBB sebanding dengan ekstrak Echinacea dan levamisol
Jeffrey K Actor - One of the best experts on this subject based on the ideXlab platform.
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cytokine production by non adherent mouse splenocyte cultures to Echinacea Extracts
Clinica Chimica Acta, 2004Co-Authors: Shenan Hwang, Jeffrey K Actor, Amitava DasguptaAbstract:Abstract Background: Echinacea is commonly used in oral dosage as an immune stimulant to increase resistance to viral, bacterial and fungal infections of the upper respiratory tract. It has been suggested that Echinacea is able to stimulate innate immune responses, including those regulated by macrophages and natural killer cells. Indeed, macrophages respond to purified polysaccharide and alkylamide preparations. However, the mechanisms for stimulation of cells responsible for adaptive immunity have not been fully elucidated for other molecules present in Echinacea purpurea preparations. Methods: Adherent and non-adherent mouse splenocyte populations were incubated in vitro with Echinacea, or with water or alcohol soluble Echinacea Extract preparations. Supernatants were collected at 48-h post-incubation, and tested by standard ELISA for presence of secreted cytokines and proinflammatory mediators. Results: Whole splenocyte populations were capable of producing significant amounts IL-6 (1014 pg/ml) in response to Echinacea preparations. The response was primarily contained towards products isolated to the water Extract preparation; no IL-6 was produced upon challenge with the alcohol Extract. The IL-6 response was produced by the non-adherent cellular population, which made 4912 pg/ml IL-6 when treated with water soluble Extract at 1 mg/ml. Likewise, the water soluble Extract of Echinacea was able to stimulate non-adherent splenocyte populations to produce TNF-α (2082 pg/ml), IL-10 (892 pg/ml) and MIP-1α (6486 pg/ml) from non-adherent splenocytes, but only significant concentrations of TNF-α and MIP-1α mediators were produced from adherent populations at similar dose concentrations. Neither population of splenocytes was capable of stimulating significant production of IFN-γ, IL-2 or IL-12 to any preparation of Echinacea examined. Conclusions: The immune stimulatory ability of components contained within E. purpurea Extracts offer insight into possible therapeutic potential of this product to regulate non-adherent lymphocytes in immune responses and activation events.
Ys Bintari - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PEMBERIAN EKSTRAK ETANOLIK RIMPANG JAHE MERAH TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Ys Bintari, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle Extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle Extract of “jahe merah” rhizome ethanolic Extract, that Extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle Extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic Extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic Extract of jahe merah was the same with Echinacea Extract and Levamisol. Jahe merupakan salah satu tanaman obat yang digunakan pada kondisi individu yang berada pada kondisi tidak nyaman; misalnya sakit kepala, diare, kembung, demam, batuk, dan masuk angin. Tujuan penelitian ini untuk mengetahui efek ekstrak zat pedas rimpang jahe merah dalam kaitannya dengan peningkatan aktivitas fagositosis makrofag. Metode penelitian dilakukan sesuai dengan metode yang pernah dilakukan oleh Leijh dkk. (1986) yaitu dengan lateks beads. Efek ekstrak zat pedas rimpang jahe merah 10 mg/kgBB, 25 mg/ kgBB, dan 100 mg/kgBB dilihat terhadap jumlah lateks yang difagositosis makrofag. Perlakuan dilakukan selama 20 hari. Pada hari ke-15, mencit diinfeksi dengan Listeria monocytogenes. Data dianalisis dengan Kolmogorov-Smirnov, dan ANOVA satu arah dengan taraf kepercayaan 95%. Hasil penelitian disimpulkan bahwa ekstrak zat pedas rimpang jahe merah 100 mg/kgBB, dengan perbandingan kadar relatif bercak zat pedas 1 dan bercak zat pedas 2 sebesar 6,8:1, kadar fenolik total 3,27% b/b EAG (Ekuivalen Asam Galat), dan IC50 14,57 mg/mL, berefek pada peningkatan aktivitas makrofag. Peningkatan fagositosis makrofag ekstrak zat pedas rimpang jahe merah 100 mg/kgBB sebanding dengan ekstrak Echinacea dan levamisol
Mellawati Dyah - One of the best experts on this subject based on the ideXlab platform.
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PENGARUH PEMBERIAN EKSTRAK ZAT PEDAS RIMPANG JAHE EMPRIT YANG DISARI DENGAN ETANOL 70% TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN YANG DIINFEKSI DENGAN Listeria monocytogenes
'Universitas Gadjah Mada', 2015Co-Authors: Mellawati Dyah, Sudarsono Sudarsono, Yuswanto AgustinusAbstract:Immunomodulator has been used as one of the attention to develop herbal medicine. Jahe is one of herbal medicine which is usually used to heal various diseases. Jahe Emprit is one of local name of Jahe in Indonesia. The aim of this research was to investigate the effect of pungent principle containing Extract of Jahe emprit rhizome on the macrophage activity compared by levamisol and Echinacea Extract. Extraction was done by 70%v/v ethanol. The concentrations of Extract were 5 mg/kg B.W; 25 mg/kg B.W; and 100 mg/kg B.W. CMC Na 1,5%w/v was used as solvent control. Levamisol 2,5 mg/kg B.W and Echinacea Extract 10 mg/kg B.W were used as positive control. The macrophage ability were measured according to Leijh et al., (1986) that was basically on the amount of latex which was captured by macrophage. The Extracts were orally administrated to the Swiss male mice at the dose of 0,2 ml/20 g B.W. The Macrophage activity was analyzed by one way ANOVA at 95% of confidence level. The Extract which had specification of 35% relative concentration of pungent principles; 3,554% ± 0,145 % W/W total phenolic substances; 13,70 mg/ml antioxydative value (IC50) at the concentrations of 5 mg/kg B.W and 25 mg/kg B.W could increase phagocytosis ability. The ability of the Extract at the concentration 25 mg/kg.B.W had no different with either the Levamizol 2,5 mg/kg. B.W., and Echinacea Extract 2,5 mg/kg. B.W.Efek immunomodulator banyak dilakukan dalam upaya pengembangan herbal medisin. Jahe merupakan salah satu bahan alami yang digunakan untuk mengatasi berbagai macam penyakit. Jahe emprit merupakan salah satu nama lokal jahe yang ada di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak zat pedas rimpang jahe emprit yang diekstraksi dengan 70% v/v etanol terhadap aktivitas makrofag, dibandingkan dengan levamisol dan ekstrak Echinacea. Ekstraksi dilakukan dengan etanol 70% v/v. Kemampuan fagositosis makrofag terhadap lateks ekstrak zat pedas rimpang jahe emprit secara in vitro pada 5 mg/kgBB; 25 mg/kgBB; dan 100 mg/kgBB. CMC Na 1,5% b/v digunakan sebagai kontrol pelarut; sedangkan sebagai kontrol positif, digunakan Levamisol 2,5 mg/kg BB dan ekstrak Ehinacea 10 mg/kg BB. Metode pengujian yang dilakukan, sesuai dengan pengujian yang dilakukan Leijh dkk (1986). Ekstrak diberikan secara oral pada mencit jantan galur Swiss sebanyak 0,2 ml/20 g BB. Data dianalisis dengan ANOVA satu jalan (taraf kepercayaan 95%). Ekstrak zat pedas rimpang jahe emprit dengan spesifikasi kadar relatif zat pedas 35%, kadar fenol total 3,554% ± 0,145 % b/b; dan bilangan antioksidan (IC50) 13,70 mg/ml, pada dosis 5 mg/kg BB dan 25 mg/kg BB dapat berefek pada peningkatan ke-mampuan fagositosis makrofag peritoneal pada mencit jantan yang diinfeksi Listeria monocytogenes. Peningkatan fagositosis ekstrak zat pedas rimpang jahe emprit dosis 25 mg/kg BB sebanding dengan imunostimulator sintetik (Levamisol hidroklorida 2,5 mg/kg BB) dan imunostimulator alami (ekstrak Echinacea 10mg/ kgBB)
