The Experts below are selected from a list of 117 Experts worldwide ranked by ideXlab platform
Holger Zimmermann - One of the best experts on this subject based on the ideXlab platform.
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integrin αvβ3 vitronectin receptor is a candidate receptor for the virulent Echovirus 9 strain barty
Journal of General Virology, 1999Co-Authors: Birgit Nelsensalz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus Echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αvβ3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after Infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this Echovirus Infection is controlled by the availability of αvβ3 integrin.
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Integrin alpha(v)beta3 (vitronectin receptor) is a candidate receptor for the virulent Echovirus 9 strain Barty.
The Journal of general virology, 1999Co-Authors: Birgit Nelsen-salz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus Echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after Infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this Echovirus Infection is controlled by the availability of alpha(v)beta3 integrin.
Kiyosu Taniguchi - One of the best experts on this subject based on the ideXlab platform.
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Short Communication Usefulness of Real-Time Reverse Transcription-Polymerase Chain Reaction for the Diagnosis of Echovirus Aseptic Meningitis Using Cerebrospinal Fluid
2020Co-Authors: Tsuguto Fujimoto, Hiroyuki Izumi, Nobuhiko Okabe, Miki Enomoto, Masami Konagaya, Masatsugu Chikahira, Tetsuya Munemura, Kiyosu TaniguchiAbstract:SUMMARY: Quantitative real-time reverse transcription-polymerase chain reaction (q-RT-PCR) was used to diagnose Echovirus Infection and the results were compared to those obtained with the viral culture rate. Cerebrospinal fluid (CSF) from a total of 40 aseptic meningitis patients was used. Positive CSF samples, determined by viral culture (n = 29), contained significantly higher Echovirus genome copy numbers (mean, 329 copies/μL) than did culture-negative CSF samples (n = 11) (mean, 34.2 copies/μL; P < 0.05). Echoviruses were identified as Echovirus serotype 9 (E-9) (n = 21); E-30 (n = 16); and E-5, E-7, and E-18 (n = 1 each) by neutralization and/ or conventional PCR-sequencing techniques. Viral culture-positive samples were collected at 1.41 ± 1.27 days after the onset of illness, and culture-negative samples were collected at 4.91 ± 3.34 days. Samples from which virus could be isolated were collected significantly earlier than were samples from which virus could not be isolated. These results strongly suggest the importance of early collection of CSF for Echovirus isolation, and demonstrate the high sensitivity of q-RT-PCR for the detection of Echoviruses in CSF.
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Usefulness of real-time reverse transcription-polymerase chain reaction for the diagnosis of Echovirus aseptic meningitis using cerebrospinal fluid.
Japanese Journal of Infectious Diseases, 2009Co-Authors: Tsuguto Fujimoto, Hiroyuki Izumi, Nobuhiko Okabe, Miki Enomoto, Masami Konagaya, Masatsugu Chikahira, Tetsuya Munemura, Kiyosu TaniguchiAbstract:Abstract Quantitative real-time reverse transcription-polymerase chain reaction (q-RT-PCR) was used to diagnose Echovirus Infection and the results were compared to those obtained with the viral culture rate. Cerebrospinal fluid (CSF) from a total of 40 aseptic meningitis patients was used. Positive CSF samples, determined by viral culture (n=29), contained significantly higher Echovirus genome copy numbers (mean, 329 copies/microL) than did culture-negative CSF samples (n=11) (mean, 34.2 copies/microL; P
Birgit Nelsensalz - One of the best experts on this subject based on the ideXlab platform.
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integrin αvβ3 vitronectin receptor is a candidate receptor for the virulent Echovirus 9 strain barty
Journal of General Virology, 1999Co-Authors: Birgit Nelsensalz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus Echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αvβ3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after Infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this Echovirus Infection is controlled by the availability of αvβ3 integrin.
Hans J Eggers - One of the best experts on this subject based on the ideXlab platform.
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integrin αvβ3 vitronectin receptor is a candidate receptor for the virulent Echovirus 9 strain barty
Journal of General Virology, 1999Co-Authors: Birgit Nelsensalz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus Echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the αvβ3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after Infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this Echovirus Infection is controlled by the availability of αvβ3 integrin.
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Integrin alpha(v)beta3 (vitronectin receptor) is a candidate receptor for the virulent Echovirus 9 strain Barty.
The Journal of general virology, 1999Co-Authors: Birgit Nelsen-salz, Hans J Eggers, Holger ZimmermannAbstract:The enterovirus Echovirus 9 strain Barty (E9/Barty) is pathogenic for newborn mice as well as for humans. In contrast to the apathogenic prototype strain Hill, strain Barty encodes an RGD motif in the C-terminal part of the structural protein VP1. Data are presented that show that E9/Barty binds its target cells via contact of the RGD motif to the alpha(v)beta3 integrin (vitronectin receptor), whereas prototype Hill uses a different, still unidentified receptor site. Furthermore, virus titres of murine muscle tissue were compared after Infection of newborn and 1-, 2-, 3- and 12-week-old mice. The replication capacity of the virus decreased dramatically with age of the infected mice. Since E9/Barty does not replicate or replicates only poorly in mice older than about 5 days, and expression of the vitronectin receptor is reported to be down-regulated in striated muscle tissue during development, it is suggested that susceptibility of mice to this Echovirus Infection is controlled by the availability of alpha(v)beta3 integrin.
Fabiana Superti - One of the best experts on this subject based on the ideXlab platform.
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Bovine lactoferrin inhibits Echovirus endocytic pathway by interacting with viral structural polypeptides
Antiviral Research, 2006Co-Authors: Maria Grazia Ammendolia, Agostina Pietrantoni, Antonella Tinari, Piera Valenti, Fabiana SupertiAbstract:Abstract Lactoferrin, an 80 kDa bi-globular iron-binding glycoprotein belonging to the transferrin family, is a pleiotropic factor with potent antimicrobial and immunomodulatory activities, present in breast milk, in mucosal secretions, and in the secondary granules of neutrophils. Recently, we have shown that bovine lactoferrin prevents the early phases of Echovirus Infection and also acts as a survival factor inhibiting viral-induced apoptosis. In the present research we investigated the mechanism of bovine lactoferrin anti-echoviral effect demonstrating that Echovirus enters susceptible cells by an endocytic pathway and that lactoferrin treatment is able to prevent viral genome delivery into the cytoplasm. It is likely that lactoferrin interaction with Echovirus capsid proteins induces alterations that stabilize the conformation of the virion making it resistant to uncoating. Taken together, the results of our study show that the inhibition of Echovirus 6 infectivity by lactoferrin is dependent on its interaction not only with cell surface glycosaminoglycan chains but also with viral structural proteins demonstrating that this glycoprotein targets the virus entry process.
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bovine lactoferrin peptidic fragments involved in inhibition of Echovirus 6 in vitro Infection
Antiviral Research, 2006Co-Authors: Agostina Pietrantoni, Maria Grazia Ammendolia, Antonella Tinari, Rosa Anna Siciliano, Piera Valenti, Fabiana SupertiAbstract:Bovine lactoferrin is a multifunctional glycoprotein folded in two symmetric globular lobes (N- and C-lobes), each being able to bind one ferric ion. We have previously demonstrated that this protein is able to prevent Echovirus-induced apoptosis. In the present study, we have investigated both the role of tryptic fragments of bovine lactoferrin and the mechanism of lactoferrin effect on Echovirus Infection. Results obtained showed that bovine lactoferrin inhibits Echovirus-induced cytopathic effect and antigen synthesis in a dose-dependent manner and that this protein is able to prevent viral replication when added not only during the entire cycle of Infection but also before, during or after the viral adsorption step. The N-terminal cationic peptide was sufficient to prevent viral binding. Our data suggest that lactoferrin inhibition of Echovirus attachment to cell receptors could be mediated by the cluster of positive charges at its N-terminus (lactoferricin).
