The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Gloria Trallero - One of the best experts on this subject based on the ideXlab platform.
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Molecular Characterization of Enteroviruses Associated With Neurological Infections in Spain, 2008
Journal of Medical Virology, 2013Co-Authors: Maria Cabrerizo, María José Mellado Peña, Gloria Trallero, Antonio Moreno-docón, Mercedes Pérez-ruiz, Juan E. Echevarría, Ana AvellónAbstract:In order to investigate the etiology of viral neurological infections in Spain, a national study was performed in 2008. The results obtained have been published. Enteroviruses were the most frequent cause of the aseptic meningitis and infant febrile syndromes. The present report supplements the previous study with the genotyping of the detected enteroviruses. Typing was by amplification of partial VP1 region and sequencing in 70 (53%) of the 132 available cerebrospinal fluid samples positive for enteroviruses. Twelve different genotypes within the B species were identified. Echovirus 4 was predominant (24%), followed by Echovirus 30 (19%), Echovirus 9 (17%), and Echovirus 6 (14%). In summary, a co-circulation of several enterovirus types associated with meningitis in children under 15 years old was observed. Although infrequently detected, Echovirus 4 was the predominant genotype identified due to an aseptic meningitis outbreak which occurred in the Canary Islands in 2008. J Med. Virol. 85:1975–1977, 2013. © 2013 Wiley Periodicals, Inc.
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Enteroviruses in Spain over the decade 1998-2007: virological and epidemiological studies.
Journal of Clinical Virology, 2009Co-Authors: Gloria Trallero, Ana Avellón, Almudena Otero, N. Rabella, T. Miguel, G. Rubio, Juan E. Echevarría, C Perez, Maria CabrerizoAbstract:Abstract Background Human enteroviruses (HEV) are the commonest cause of viral meningitis as well as other pathologies, therefore HEV characterization is important both in patient management and epidemiological investigation. Objectives A 10-year study of patients with enteroviral infection was carried out in Spain to determine the underlying etiology. Study design HEV were fully typed by microneutralisation tests and/or molecular methods. Results A collection of 86404 clinical samples were studied in several Spanish laboratories. These were collected from patients with different syndromes, mainly aseptic meningitis (AM), fever, respiratory diseases and acute flaccid paralysis. Of these, 6867 HEV were obtained. At the National Poliovirus Laboratory 2814 were serotypically characterised. Among non-polio enteroviruses, the eight main serotypes were Echovirus 30 (25%), Echovirus 6 (12.4%), Echovirus 13 (8.3%), Echovirus 11 (7.4%) and Echovirus 9 (4.7%), followed by Coxsackievirus B5 (4.2%) and Echovirus 7 and Coxsackievirus A9 (3.7%) each. In AM cases, Echovirus 30 was identified in 39% of them, followed by Echovirus 6 (14%). However, Echovirus 6 was mainly associated with respiratory disease (17%), followed by Echovirus 11 (10%). On the other hand, Echovirus 30, Echovirus 11 and Echovirus 6 contributed equally with 12% of each serotype in the cases of fever. Conclusions The present report complements previous data (Trallero et al. 13 ), with the results of HEV incidence in Spain from 1998 to 2007. The surveillance described in this study provided valuable information as to which serotypes are in circulation, the emergence of new HEV and association with clinical manifestations.
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Molecular epidemiological study of HEV-B enteroviruses involved in the increase in meningitis cases occurred in Spain during 2006.
Journal of medical virology, 2008Co-Authors: Maria Cabrerizo, Juan E. Echevarría, Irene González, Teresa De Miguel, Gloria TralleroAbstract:Human enteroviruses are one of the main etiological agents of aseptic meningitis and other central nervous system infections, particularly the serotypes included in the enterovirus B species. Molecular methods have proved useful to identify serotypes in clinical samples, facilitating the epidemiological study of these viruses. In the spring of 2006, there was a significant increase in meningitis cases caused by enteroviruses in Spain. In the present study, 138 enteroviruses directly detected in clinical samples of patients with aseptic meningitis (n = 116) and other neurological pathologies (n = 22) received by the National Center for Microbiology during the year, were genotyped by amplification and sequencing part of the VP1 region and phylogenetic analysis. Echovirus 30 was the most frequent serotype, followed in decreasing order by Echovirus 6, 9, 13, 18, enterovirus 75, coxsackievirus A9, Echovirus 11, 14, 29, 4, and coxsackievirus B4 and B5. Phylogenetic analysis with all Spanish Echovirus 30 strains detected in 2006 and other reported Echovirus 30 sequences, demonstrated that Spanish strains formed a new lineage, different from others previously described. In conclusion, Echovirus 30 is the most commonly reported enterovirus serotype associated with aseptic meningitis in Spain. Direct molecular typing of clinical samples also allows rapid identification of the serotypes involved in an epidemic alert and phylogenetic analysis in the 3'-VP1 region is useful to study viral epidemiology.
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Enteroviruses in Spain: virological and epidemiological studies over 10 years (1988-97).
Epidemiology and infection, 2000Co-Authors: Gloria Trallero, Inmaculada Casas, Antonio Tenorio, Juan Emilio Echevarría, A. Castellanos, Lozano A, Pilar Pérez BreñaAbstract:SUMMARY A total of 15662 clinical samples were analysed for enterovirus (EV) isolation in cell cultures during a 10-year period (1988‐97). Furthermore, 210 isolates of EV obtained in primary laboratories within Spain from patients with meningitis were characterized. The total number of EV typed was 758, including 727 non-polio EV and 31 Sabin-like (SL) polioviruses. Twentyeight EV serotypes were represented. Echoviruses comprised 90% (653}727) of fully typed nonpolio EV. The four most prevalent serotypes were Echovirus 30, Echovirus 9, Echovirus 6 and Echovirus 4. Echovirus 30 was the main serotype associated with meningitis. Echovirus 9 was the aetiological agent in 20 outbreaks of meningitis while the occurrence of Echovirus 6 was localized in 1 year (1997). Coxsackieviruses A and B occurred in 3 and 7% of the non-polio EV respectively. Coxsackievirus B5 presented the relative greater abundance. This paper examines the epidemiology of EV in Spain to serotype level over a 10-year period with special attention to non-polio EV associated with meningitis.
Sabine Diedrich - One of the best experts on this subject based on the ideXlab platform.
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Meningitis gone viral: description of the Echovirus wave 2013 in Germany
BMC Infectious Diseases, 2019Co-Authors: Jonas Graf, Christian J. Hartmann, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Sabine DiedrichAbstract:Background Aseptic meningitis epidemics may pose various health care challenges. Methods We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. Results A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and 1 EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. Conclusions Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: description of the Echovirus wave 2013 in Germany
BMC infectious diseases, 2019Co-Authors: Jonas Graf, Christian J. Hartmann, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Helmar C. Lehmann, Christian A. Schneider, Sabine DiedrichAbstract:Aseptic meningitis epidemics may pose various health care challenges. We describe the German enterovirus meningitis epidemics in the university hospital centers of Dusseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and 1 EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: Description of the Echovirus Wave 2013 in Germany
2019Co-Authors: Jonas Graf, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Christian Hartmann, Sabine DiedrichAbstract:Abstract BACKGROUND: Aseptic meningitis epidemics may pose various health care challenges. METHODS: We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases.RESULTS: A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and one EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. CONCLUSIONS: Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: Description of the Echovirus Wave 2013 in Germany
2019Co-Authors: Jonas Graf, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Christian Hartmann, Sabine DiedrichAbstract:Abstract BACKGROUND: Aseptic meningitis epidemics may pose various health care challenges. OBJECTIVE: We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1 st and December 31 st , 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. RESULTS: A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and one EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. CONCLUSION: Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
Maria Cabrerizo - One of the best experts on this subject based on the ideXlab platform.
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Molecular Characterization of Enteroviruses Associated With Neurological Infections in Spain, 2008
Journal of Medical Virology, 2013Co-Authors: Maria Cabrerizo, María José Mellado Peña, Gloria Trallero, Antonio Moreno-docón, Mercedes Pérez-ruiz, Juan E. Echevarría, Ana AvellónAbstract:In order to investigate the etiology of viral neurological infections in Spain, a national study was performed in 2008. The results obtained have been published. Enteroviruses were the most frequent cause of the aseptic meningitis and infant febrile syndromes. The present report supplements the previous study with the genotyping of the detected enteroviruses. Typing was by amplification of partial VP1 region and sequencing in 70 (53%) of the 132 available cerebrospinal fluid samples positive for enteroviruses. Twelve different genotypes within the B species were identified. Echovirus 4 was predominant (24%), followed by Echovirus 30 (19%), Echovirus 9 (17%), and Echovirus 6 (14%). In summary, a co-circulation of several enterovirus types associated with meningitis in children under 15 years old was observed. Although infrequently detected, Echovirus 4 was the predominant genotype identified due to an aseptic meningitis outbreak which occurred in the Canary Islands in 2008. J Med. Virol. 85:1975–1977, 2013. © 2013 Wiley Periodicals, Inc.
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Enteroviruses in Spain over the decade 1998-2007: virological and epidemiological studies.
Journal of Clinical Virology, 2009Co-Authors: Gloria Trallero, Ana Avellón, Almudena Otero, N. Rabella, T. Miguel, G. Rubio, Juan E. Echevarría, C Perez, Maria CabrerizoAbstract:Abstract Background Human enteroviruses (HEV) are the commonest cause of viral meningitis as well as other pathologies, therefore HEV characterization is important both in patient management and epidemiological investigation. Objectives A 10-year study of patients with enteroviral infection was carried out in Spain to determine the underlying etiology. Study design HEV were fully typed by microneutralisation tests and/or molecular methods. Results A collection of 86404 clinical samples were studied in several Spanish laboratories. These were collected from patients with different syndromes, mainly aseptic meningitis (AM), fever, respiratory diseases and acute flaccid paralysis. Of these, 6867 HEV were obtained. At the National Poliovirus Laboratory 2814 were serotypically characterised. Among non-polio enteroviruses, the eight main serotypes were Echovirus 30 (25%), Echovirus 6 (12.4%), Echovirus 13 (8.3%), Echovirus 11 (7.4%) and Echovirus 9 (4.7%), followed by Coxsackievirus B5 (4.2%) and Echovirus 7 and Coxsackievirus A9 (3.7%) each. In AM cases, Echovirus 30 was identified in 39% of them, followed by Echovirus 6 (14%). However, Echovirus 6 was mainly associated with respiratory disease (17%), followed by Echovirus 11 (10%). On the other hand, Echovirus 30, Echovirus 11 and Echovirus 6 contributed equally with 12% of each serotype in the cases of fever. Conclusions The present report complements previous data (Trallero et al. 13 ), with the results of HEV incidence in Spain from 1998 to 2007. The surveillance described in this study provided valuable information as to which serotypes are in circulation, the emergence of new HEV and association with clinical manifestations.
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Molecular epidemiological study of HEV-B enteroviruses involved in the increase in meningitis cases occurred in Spain during 2006.
Journal of medical virology, 2008Co-Authors: Maria Cabrerizo, Juan E. Echevarría, Irene González, Teresa De Miguel, Gloria TralleroAbstract:Human enteroviruses are one of the main etiological agents of aseptic meningitis and other central nervous system infections, particularly the serotypes included in the enterovirus B species. Molecular methods have proved useful to identify serotypes in clinical samples, facilitating the epidemiological study of these viruses. In the spring of 2006, there was a significant increase in meningitis cases caused by enteroviruses in Spain. In the present study, 138 enteroviruses directly detected in clinical samples of patients with aseptic meningitis (n = 116) and other neurological pathologies (n = 22) received by the National Center for Microbiology during the year, were genotyped by amplification and sequencing part of the VP1 region and phylogenetic analysis. Echovirus 30 was the most frequent serotype, followed in decreasing order by Echovirus 6, 9, 13, 18, enterovirus 75, coxsackievirus A9, Echovirus 11, 14, 29, 4, and coxsackievirus B4 and B5. Phylogenetic analysis with all Spanish Echovirus 30 strains detected in 2006 and other reported Echovirus 30 sequences, demonstrated that Spanish strains formed a new lineage, different from others previously described. In conclusion, Echovirus 30 is the most commonly reported enterovirus serotype associated with aseptic meningitis in Spain. Direct molecular typing of clinical samples also allows rapid identification of the serotypes involved in an epidemic alert and phylogenetic analysis in the 3'-VP1 region is useful to study viral epidemiology.
Carolyn B Coyne - One of the best experts on this subject based on the ideXlab platform.
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Human FcRn expression and Type I Interferon signaling control Echovirus 11 pathogenesis in mice.
PLoS pathogens, 2021Co-Authors: Alexandra I. Wells, Christopher J Bakkenist, Kalena A. Grimes, Kenneth Kim, Emilie Branche, William H. Depas, Sujan Shresta, Carolyn B CoyneAbstract:Neonatal Echovirus infections are characterized by severe hepatitis and neurological complications that can be fatal. Here, we show that expression of the human homologue of the neonatal Fc receptor (hFcRn), the primary receptor for Echoviruses, and ablation of type I interferon (IFN) signaling are key host determinants involved in Echovirus pathogenesis. We show that expression of hFcRn alone is insufficient to confer susceptibility to Echovirus infections in mice. However, expression of hFcRn in mice deficient in type I interferon (IFN) signaling, hFcRn-IFNAR-/-, recapitulate the Echovirus pathogenesis observed in humans. Luminex-based multianalyte profiling from E11 infected hFcRn-IFNAR-/- mice revealed a robust systemic immune response to infection, including the induction of type I IFNs. Furthermore, similar to the severe hepatitis observed in humans, E11 infection in hFcRn-IFNAR-/- mice caused profound liver damage. Our findings define the host factors involved in Echovirus pathogenesis and establish in vivo models that recapitulate Echovirus disease in humans.
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The neonatal Fc receptor is a pan-Echovirus receptor
Proceedings of the National Academy of Sciences of the United States of America, 2019Co-Authors: Stefanie A Morosky, Kathryn Lemon, Azia S. Evans, Sandra Schamus, Alexandra I. Wells, Christopher J Bakkenist, Carolyn B CoyneAbstract:Echoviruses are amongst the most common causative agents of aseptic meningitis worldwide and are particularly devastating in the neonatal population, where they are associated with severe hepatitis, neurological disease, including meningitis and encephalitis, and even death. Here, we identify the neonatal Fc receptor (FcRn) as a pan-Echovirus receptor. We show that loss of expression of FcRn or its binding partner beta 2 microglobulin (β2M) renders cells resistant to infection by a panel of Echoviruses at the stage of virus attachment, and that a blocking antibody to β2M inhibits Echovirus infection in cell lines and in primary human intestinal epithelial cells. We also show that expression of human, but not mouse, FcRn renders nonpermissive human and mouse cells sensitive to Echovirus infection and that the extracellular domain of human FcRn directly binds Echovirus particles and neutralizes infection. Lastly, we show that neonatal mice expressing human FcRn are more susceptible to Echovirus infection by the enteral route. Our findings thus identify FcRn as a pan-Echovirus receptor, which may explain the enhanced susceptibility of neonates to Echovirus infections.
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The neonatal Fc receptor is a pan-Echovirus receptor
bioRxiv, 2018Co-Authors: Stefanie A Morosky, Kathryn Lemon, Azia S. Evans, Sandra Schmus, Christopher J Bakkenist, Carolyn B CoyneAbstract:Echoviruses are the main causative agents of aseptic meningitis worldwide and are particularly devastating in the neonatal population, where they are associated with severe hepatitis, neurological disease including meningitis and encephalitis, and even death. Here, we identify the neonatal Fc receptor (FcRn) as a pan-Echovirus receptor. We show that loss of expression of FcRn or its binding partner beta 2 microglubulin (B2M) renders human brain microvascular cells resistant to infection by a panel of Echoviruses at the stage of virus attachment and that a blocking antibody to B2M inhibit Echovirus infection in cell lines and in primary human fetal intestinal epithelial cells. We also show that expression of human, but not mouse, FcRn renders non-permissive human and mouse cells sensitive to Echovirus infection and that the extracellular domain of human FcRn directly binds echoviral particles and neutralizes infection. Lastly, we show that primary cells isolated from mice that express human FcRn are highly susceptible to Echovirus infection. Our findings thus identify FcRn as a pan-Echovirus receptor, which may explain the enhanced susceptibility of neonates to Echovirus infections.
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The neonatal Fc receptor is a pan-Echovirus receptor
2018Co-Authors: Stefanie A Morosky, Azia S. Evans, Sandra Schamus, Christopher J Bakkenist, Kathryn L. Lemon, Carolyn B CoyneAbstract:AbstractEchoviruses are the main causative agents of aseptic meningitis worldwide and are particularly devastating in the neonatal population, where they are associated with severe hepatitis, neurological disease including meningitis and encephalitis, and even death. Here, we identify the neonatal Fc receptor (FcRn) as a pan-Echovirus receptor. We show that loss of expression of FcRn or its binding partner beta 2 microglubulin (β2M) renders human brain microvascular cells resistant to infection by a panel of Echoviruses at the stage of virus attachment and that a blocking antibody to β2M inhibit Echovirus infection in cell lines and in primary human fetal intestinal epithelial cells. We also show that expression of human, but not mouse, FcRn renders non-permissive human and mouse cells sensitive to Echovirus infection and that the extracellular domain of human FcRn directly binds echoviral particles and neutralizes infection. Lastly, we show that primary cells isolated from mice that express human FcRn are highly susceptible to Echovirus infection. Our findings thus identify FcRn as a pan-Echovirus receptor, which may explain the enhanced susceptibility of neonates to Echovirus infections.SignificanceEchoviruses are associated with aseptic meningitis and induce severe disease, and even death, in neonates and young infants. Here, we identify the neonatal Fc receptor (FcRn) as a pan-Echovirus receptor. FcRn is expressed on the surface of the human placenta, and throughout life in intestinal enterocytes, liver hepatocytes, and in the microvascular endothelial cells that line the blood-brain barrier. This pattern of expression is consistent with the organ sites targeted by Echoviruses in humans, with the primary entry site of infection in the intestinal tract and subsequent infection of secondary tissues including the liver and brain. These findings provide important insights into Echovirus pathogenesis and may explain the enhanced susceptibility of infants and neonates to Echovirus-induced disease.
Jonas Graf - One of the best experts on this subject based on the ideXlab platform.
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Meningitis gone viral: description of the Echovirus wave 2013 in Germany
BMC Infectious Diseases, 2019Co-Authors: Jonas Graf, Christian J. Hartmann, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Sabine DiedrichAbstract:Background Aseptic meningitis epidemics may pose various health care challenges. Methods We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. Results A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and 1 EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. Conclusions Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: description of the Echovirus wave 2013 in Germany
BMC infectious diseases, 2019Co-Authors: Jonas Graf, Christian J. Hartmann, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Helmar C. Lehmann, Christian A. Schneider, Sabine DiedrichAbstract:Aseptic meningitis epidemics may pose various health care challenges. We describe the German enterovirus meningitis epidemics in the university hospital centers of Dusseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and 1 EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: Description of the Echovirus Wave 2013 in Germany
2019Co-Authors: Jonas Graf, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Christian Hartmann, Sabine DiedrichAbstract:Abstract BACKGROUND: Aseptic meningitis epidemics may pose various health care challenges. METHODS: We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1st and December 31st, 2013 in order to scrutinize clinical differences from other aseptic meningitis cases.RESULTS: A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and one EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. CONCLUSIONS: Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
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Meningitis gone viral: Description of the Echovirus Wave 2013 in Germany
2019Co-Authors: Jonas Graf, Carolin Otto, Hans Martin Bosse, Ortwin Adams, Klemens Ruprecht, Michael Karenfort, Christian Schneider, Helmar C. Lehmann, Christian Hartmann, Sabine DiedrichAbstract:Abstract BACKGROUND: Aseptic meningitis epidemics may pose various health care challenges. OBJECTIVE: We describe the German enterovirus meningitis epidemics in the university hospital centers of Düsseldorf, Cologne and Berlin between January 1 st and December 31 st , 2013 in order to scrutinize clinical differences from other aseptic meningitis cases. RESULTS: A total of 72 enterovirus (EV-positive) meningitis cases were detected in our multicenter cohort, corresponding to 5.8% of all EV-positive cases which were voluntarily reported within the National Enterovirus surveillance (EVSurv, based on investigation of patients with suspected aseptic meningitis/encephalitis and/or acute flaccid paralysis) by physicians within this period of time. Among these 72 patients, 38 (52.8%) were enterovirus positive and typed as Echovirus (18 pediatric and 20 adult cases, median age 18.5 years; Echovirus 18 (1), Echovirus 2 (1), Echovirus 30 (31), Echovirus 33 (1), Echovirus 9 (4)). At the same time, 45 aseptic meningitis cases in our cohort were excluded to be due to enteroviral infection (EV-negative). Three EV-negative patients were tested positive for varicella zoster virus (VZV) and one EV-negative patient for herpes simplex virus 2. Hospitalization was significantly longer in EV-negative cases. Cerebrospinal fluid analysis did not reveal significant differences between the two groups. After discharge, EV-meningitis resulted in significant burden of sick leave in our pediatric cohort as parents had to care for the children at home. CONCLUSION: Voluntary syndromic surveillance, such as provided by the EVSurv in our study may be a valuable tool for epidemiological research. Our analyses suggest that EV-positive meningitis predominantly affects younger patients and may be associated with a rather benign clinical course, compared to EV-negative cases.
