The Experts below are selected from a list of 414981 Experts worldwide ranked by ideXlab platform
Eaqub Ali - One of the best experts on this subject based on the ideXlab platform.
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeti...
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using species-specific primers targeting mitochondrial cytb, ND5, and 16s rRNA genes. Assay validation of adulterated and various heat-treated meatball matrices showed excellent stability and sensitivity under all processing conditions. The detection limit was 0.01–0.001 ng of DNA under pure states and 0.5% meat in meatball products. Buffalo was detected in 86.7% (13 out of 15) of tested commercial beef products, while chicken, pork, and fish products were found to be pure. The developed assay was efficient enough to detect target species simultaneously, even in highly degraded and processed food products at reduced time.
M Motalib A Hossain - One of the best experts on this subject based on the ideXlab platform.
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeti...
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using species-specific primers targeting mitochondrial cytb, ND5, and 16s rRNA genes. Assay validation of adulterated and various heat-treated meatball matrices showed excellent stability and sensitivity under all processing conditions. The detection limit was 0.01–0.001 ng of DNA under pure states and 0.5% meat in meatball products. Buffalo was detected in 86.7% (13 out of 15) of tested commercial beef products, while chicken, pork, and fish products were found to be pure. The developed assay was efficient enough to detect target species simultaneously, even in highly degraded and processed food products at reduced time.
Sharmin Sultana - One of the best experts on this subject based on the ideXlab platform.
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeti...
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using species-specific primers targeting mitochondrial cytb, ND5, and 16s rRNA genes. Assay validation of adulterated and various heat-treated meatball matrices showed excellent stability and sensitivity under all processing conditions. The detection limit was 0.01–0.001 ng of DNA under pure states and 0.5% meat in meatball products. Buffalo was detected in 86.7% (13 out of 15) of tested commercial beef products, while chicken, pork, and fish products were found to be pure. The developed assay was efficient enough to detect target species simultaneously, even in highly degraded and processed food products at reduced time.
Mohd Rafie Johan - One of the best experts on this subject based on the ideXlab platform.
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeti...
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using species-specific primers targeting mitochondrial cytb, ND5, and 16s rRNA genes. Assay validation of adulterated and various heat-treated meatball matrices showed excellent stability and sensitivity under all processing conditions. The detection limit was 0.01–0.001 ng of DNA under pure states and 0.5% meat in meatball products. Buffalo was detected in 86.7% (13 out of 15) of tested commercial beef products, while chicken, pork, and fish products were found to be pure. The developed assay was efficient enough to detect target species simultaneously, even in highly degraded and processed food products at reduced time.
Zaira Zaman Chowdhury - One of the best experts on this subject based on the ideXlab platform.
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeti...
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heptaplex polymerase chain reaction assay for the simultaneous detection of beef buffalo chicken cat dog pork and fish in raw and heat treated food products
2019Co-Authors: M Motalib A Hossain, Syed Muhammad Kamal Uddin, Sharmin Sultana, Sharmin Quazi Bonny, Firoz Khan, Zaira Zaman Chowdhury, Mohd Rafie Johan, Eaqub AliAbstract:Species authentication of meat and fish products is crucial to safeguard public health, Economic Investment, and religious sanctity. We developed a heptaplex polymerase chain reaction assay targeting short amplicon length (73–198 bp) for the simultaneous detection and differentiation of cow, buffalo, chicken, cat, dog, pig, and fish species in raw and processed food using species-specific primers targeting mitochondrial cytb, ND5, and 16s rRNA genes. Assay validation of adulterated and various heat-treated meatball matrices showed excellent stability and sensitivity under all processing conditions. The detection limit was 0.01–0.001 ng of DNA under pure states and 0.5% meat in meatball products. Buffalo was detected in 86.7% (13 out of 15) of tested commercial beef products, while chicken, pork, and fish products were found to be pure. The developed assay was efficient enough to detect target species simultaneously, even in highly degraded and processed food products at reduced time.
