The Experts below are selected from a list of 2586 Experts worldwide ranked by ideXlab platform
Keisuke Toyama - One of the best experts on this subject based on the ideXlab platform.
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Ecotropic Virus Integration Site-l Gene Preferentially Expressed in Post-Myelodysplasia Acute Myeloid Leukemia: Possible Association With GATA-1, GATA-2, and Stem Cell Leukemia Gene Expression
2016Co-Authors: H. Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-l (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse tran-scriptase-polymerase chain reaction (RT-PCR) method. The EVl l transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVl l expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryo-blast crisis. No EVl l transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EV l l gene is located, were found in on
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Ecotropic Virus integration site 1 gene preferentially expressed in post myelodysplasia acute myeloid leukemia possible association with gata 1 gata 2 and stem cell leukemia gene expression
Blood, 1995Co-Authors: Junko H Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-1 (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. The EVI1 transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVI1 expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryoblast crisis. No EVI1 transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EVI1 gene is located, were found in one patient with MDS and two patients with CML myelomegakaryoblast crisis who had EVI1 expression. Our results showed that EVI1 expression was frequent in patients with post-MDS AML and AML with trilineage myelodysplasia, regardless of the presence or absence of 3q26 abnormalities. EVI1 expression was accompanied by expression of GATA-1 and GATA-2, and often by stem cell leukemia (SCL) gene expression. In patients with post-MDS AML, EVI1 expression was not always associated with a 3q26 abnormality, whereas EVI1 expression in CML myelomegakaryoblast crisis was often linked to a 3q26 abnormality. Our results suggest that the leukemogenic role of EVI1 expression may differ between post-MDS AML and leukemia, with EVI1 expression associated with a 3q26 abnormality.
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evi1 expression associated with a 3q26 anomaly in a leukemia cell line derived from the blast crisis of chronic myeloid leukemia
Leukemia, 1994Co-Authors: Kazuma Ohyashiki, Takashi Shimamoto, Junko H Ohyashiki, Shinpei Nakazawa, H Fujieda, Ken Kawakubo, K Suzukawa, K Morishita, Keisuke ToyamaAbstract:Two leukemia cell lines, TS9;22 and YS9;22, were established from different individuals with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia in blast crisis. The reverse transcript-polymerase chain reaction (RT-PCR) technique revealed that both cell lines expressed GATA-1, GATA-2, and the stem cell leukemia (SCL) gene, consistent with a megakaryocyte lineage. Chromosome analysis revealed that TS9;22 cells show the Ph translocation without abnormality of chromosome 3. In contrast, YS9;22 cells show the Ph translocation and dic(3)(q26;p12). Northern analysis revealed that YS9;22 cells express the EVI1 (Ecotropic Virus integration-1) gene, possibly because of the chromosomal translocation in the 3q26 region; TS9;22 cells do not express EVI1. However, no rearrangements were detected over 600 kb upstream or over 900 kb downstream of EVI1 in the YS9;22 cell line, suggesting a different mechanism of EVI1 activation from that in leukemia cells with either a t(3;3)(q21;q26) or inv(3)(q21q26). These results indicate that EVI1 expression in YS9;22 cells is linked to the 3q26 abnormality and that EVI1 activation plays an oncogenic role in the blastic transformation of chronic myeloid leukemia.
Kazuma Ohyashiki - One of the best experts on this subject based on the ideXlab platform.
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Ecotropic Virus Integration Site-l Gene Preferentially Expressed in Post-Myelodysplasia Acute Myeloid Leukemia: Possible Association With GATA-1, GATA-2, and Stem Cell Leukemia Gene Expression
2016Co-Authors: H. Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-l (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse tran-scriptase-polymerase chain reaction (RT-PCR) method. The EVl l transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVl l expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryo-blast crisis. No EVl l transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EV l l gene is located, were found in on
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Ecotropic Virus integration site 1 gene preferentially expressed in post myelodysplasia acute myeloid leukemia possible association with gata 1 gata 2 and stem cell leukemia gene expression
Blood, 1995Co-Authors: Junko H Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-1 (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. The EVI1 transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVI1 expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryoblast crisis. No EVI1 transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EVI1 gene is located, were found in one patient with MDS and two patients with CML myelomegakaryoblast crisis who had EVI1 expression. Our results showed that EVI1 expression was frequent in patients with post-MDS AML and AML with trilineage myelodysplasia, regardless of the presence or absence of 3q26 abnormalities. EVI1 expression was accompanied by expression of GATA-1 and GATA-2, and often by stem cell leukemia (SCL) gene expression. In patients with post-MDS AML, EVI1 expression was not always associated with a 3q26 abnormality, whereas EVI1 expression in CML myelomegakaryoblast crisis was often linked to a 3q26 abnormality. Our results suggest that the leukemogenic role of EVI1 expression may differ between post-MDS AML and leukemia, with EVI1 expression associated with a 3q26 abnormality.
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evi1 expression associated with a 3q26 anomaly in a leukemia cell line derived from the blast crisis of chronic myeloid leukemia
Leukemia, 1994Co-Authors: Kazuma Ohyashiki, Takashi Shimamoto, Junko H Ohyashiki, Shinpei Nakazawa, H Fujieda, Ken Kawakubo, K Suzukawa, K Morishita, Keisuke ToyamaAbstract:Two leukemia cell lines, TS9;22 and YS9;22, were established from different individuals with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia in blast crisis. The reverse transcript-polymerase chain reaction (RT-PCR) technique revealed that both cell lines expressed GATA-1, GATA-2, and the stem cell leukemia (SCL) gene, consistent with a megakaryocyte lineage. Chromosome analysis revealed that TS9;22 cells show the Ph translocation without abnormality of chromosome 3. In contrast, YS9;22 cells show the Ph translocation and dic(3)(q26;p12). Northern analysis revealed that YS9;22 cells express the EVI1 (Ecotropic Virus integration-1) gene, possibly because of the chromosomal translocation in the 3q26 region; TS9;22 cells do not express EVI1. However, no rearrangements were detected over 600 kb upstream or over 900 kb downstream of EVI1 in the YS9;22 cell line, suggesting a different mechanism of EVI1 activation from that in leukemia cells with either a t(3;3)(q21;q26) or inv(3)(q21q26). These results indicate that EVI1 expression in YS9;22 cells is linked to the 3q26 abnormality and that EVI1 activation plays an oncogenic role in the blastic transformation of chronic myeloid leukemia.
Ken Kawakubo - One of the best experts on this subject based on the ideXlab platform.
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Ecotropic Virus Integration Site-l Gene Preferentially Expressed in Post-Myelodysplasia Acute Myeloid Leukemia: Possible Association With GATA-1, GATA-2, and Stem Cell Leukemia Gene Expression
2016Co-Authors: H. Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-l (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse tran-scriptase-polymerase chain reaction (RT-PCR) method. The EVl l transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVl l expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryo-blast crisis. No EVl l transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EV l l gene is located, were found in on
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Ecotropic Virus integration site 1 gene preferentially expressed in post myelodysplasia acute myeloid leukemia possible association with gata 1 gata 2 and stem cell leukemia gene expression
Blood, 1995Co-Authors: Junko H Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-1 (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. The EVI1 transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVI1 expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryoblast crisis. No EVI1 transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EVI1 gene is located, were found in one patient with MDS and two patients with CML myelomegakaryoblast crisis who had EVI1 expression. Our results showed that EVI1 expression was frequent in patients with post-MDS AML and AML with trilineage myelodysplasia, regardless of the presence or absence of 3q26 abnormalities. EVI1 expression was accompanied by expression of GATA-1 and GATA-2, and often by stem cell leukemia (SCL) gene expression. In patients with post-MDS AML, EVI1 expression was not always associated with a 3q26 abnormality, whereas EVI1 expression in CML myelomegakaryoblast crisis was often linked to a 3q26 abnormality. Our results suggest that the leukemogenic role of EVI1 expression may differ between post-MDS AML and leukemia, with EVI1 expression associated with a 3q26 abnormality.
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evi1 expression associated with a 3q26 anomaly in a leukemia cell line derived from the blast crisis of chronic myeloid leukemia
Leukemia, 1994Co-Authors: Kazuma Ohyashiki, Takashi Shimamoto, Junko H Ohyashiki, Shinpei Nakazawa, H Fujieda, Ken Kawakubo, K Suzukawa, K Morishita, Keisuke ToyamaAbstract:Two leukemia cell lines, TS9;22 and YS9;22, were established from different individuals with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia in blast crisis. The reverse transcript-polymerase chain reaction (RT-PCR) technique revealed that both cell lines expressed GATA-1, GATA-2, and the stem cell leukemia (SCL) gene, consistent with a megakaryocyte lineage. Chromosome analysis revealed that TS9;22 cells show the Ph translocation without abnormality of chromosome 3. In contrast, YS9;22 cells show the Ph translocation and dic(3)(q26;p12). Northern analysis revealed that YS9;22 cells express the EVI1 (Ecotropic Virus integration-1) gene, possibly because of the chromosomal translocation in the 3q26 region; TS9;22 cells do not express EVI1. However, no rearrangements were detected over 600 kb upstream or over 900 kb downstream of EVI1 in the YS9;22 cell line, suggesting a different mechanism of EVI1 activation from that in leukemia cells with either a t(3;3)(q21;q26) or inv(3)(q21q26). These results indicate that EVI1 expression in YS9;22 cells is linked to the 3q26 abnormality and that EVI1 activation plays an oncogenic role in the blastic transformation of chronic myeloid leukemia.
Takashi Shimamoto - One of the best experts on this subject based on the ideXlab platform.
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Ecotropic Virus Integration Site-l Gene Preferentially Expressed in Post-Myelodysplasia Acute Myeloid Leukemia: Possible Association With GATA-1, GATA-2, and Stem Cell Leukemia Gene Expression
2016Co-Authors: H. Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-l (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse tran-scriptase-polymerase chain reaction (RT-PCR) method. The EVl l transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVl l expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryo-blast crisis. No EVl l transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EV l l gene is located, were found in on
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Ecotropic Virus integration site 1 gene preferentially expressed in post myelodysplasia acute myeloid leukemia possible association with gata 1 gata 2 and stem cell leukemia gene expression
Blood, 1995Co-Authors: Junko H Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-1 (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. The EVI1 transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVI1 expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryoblast crisis. No EVI1 transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EVI1 gene is located, were found in one patient with MDS and two patients with CML myelomegakaryoblast crisis who had EVI1 expression. Our results showed that EVI1 expression was frequent in patients with post-MDS AML and AML with trilineage myelodysplasia, regardless of the presence or absence of 3q26 abnormalities. EVI1 expression was accompanied by expression of GATA-1 and GATA-2, and often by stem cell leukemia (SCL) gene expression. In patients with post-MDS AML, EVI1 expression was not always associated with a 3q26 abnormality, whereas EVI1 expression in CML myelomegakaryoblast crisis was often linked to a 3q26 abnormality. Our results suggest that the leukemogenic role of EVI1 expression may differ between post-MDS AML and leukemia, with EVI1 expression associated with a 3q26 abnormality.
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evi1 expression associated with a 3q26 anomaly in a leukemia cell line derived from the blast crisis of chronic myeloid leukemia
Leukemia, 1994Co-Authors: Kazuma Ohyashiki, Takashi Shimamoto, Junko H Ohyashiki, Shinpei Nakazawa, H Fujieda, Ken Kawakubo, K Suzukawa, K Morishita, Keisuke ToyamaAbstract:Two leukemia cell lines, TS9;22 and YS9;22, were established from different individuals with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia in blast crisis. The reverse transcript-polymerase chain reaction (RT-PCR) technique revealed that both cell lines expressed GATA-1, GATA-2, and the stem cell leukemia (SCL) gene, consistent with a megakaryocyte lineage. Chromosome analysis revealed that TS9;22 cells show the Ph translocation without abnormality of chromosome 3. In contrast, YS9;22 cells show the Ph translocation and dic(3)(q26;p12). Northern analysis revealed that YS9;22 cells express the EVI1 (Ecotropic Virus integration-1) gene, possibly because of the chromosomal translocation in the 3q26 region; TS9;22 cells do not express EVI1. However, no rearrangements were detected over 600 kb upstream or over 900 kb downstream of EVI1 in the YS9;22 cell line, suggesting a different mechanism of EVI1 activation from that in leukemia cells with either a t(3;3)(q21;q26) or inv(3)(q21q26). These results indicate that EVI1 expression in YS9;22 cells is linked to the 3q26 abnormality and that EVI1 activation plays an oncogenic role in the blastic transformation of chronic myeloid leukemia.
Shinpei Nakazawa - One of the best experts on this subject based on the ideXlab platform.
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Ecotropic Virus Integration Site-l Gene Preferentially Expressed in Post-Myelodysplasia Acute Myeloid Leukemia: Possible Association With GATA-1, GATA-2, and Stem Cell Leukemia Gene Expression
2016Co-Authors: H. Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-l (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse tran-scriptase-polymerase chain reaction (RT-PCR) method. The EVl l transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVl l expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryo-blast crisis. No EVl l transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EV l l gene is located, were found in on
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Ecotropic Virus integration site 1 gene preferentially expressed in post myelodysplasia acute myeloid leukemia possible association with gata 1 gata 2 and stem cell leukemia gene expression
Blood, 1995Co-Authors: Junko H Ohyashiki, Takashi Shimamoto, Kazuma Ohyashiki, Shinpei Nakazawa, Ken Kawakubo, Toshikatsu Fujimura, Keisuke ToyamaAbstract:We investigated expression of the human Ecotropic Virus integration site-1 (EVI1) gene in patients with leukemia and myelodysplastic syndrome (MDS) using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. The EVI1 transcripts were detected in 5 (10.0%) of 50 patients with de novo acute myeloid leukemia (AML), including two AML patients with trilineage myelodysplasia, and in 8 (34.8%) of 23 patients with post-myelodysplastic syndrome AML (post-MDS AML). EVI1 expression was also detected in 6 (35.3%) of 17 MDS patients and three of six patients with chronic myeloid leukemia (CML) in myelomegakaryoblast crisis. No EVI1 transcripts were detected in patients with acute lymphoid leukemia (n = 15) or CML in lymphoid blast crisis (n = 4). Chromosomal abnormalities at the 3q26 region, where the EVI1 gene is located, were found in one patient with MDS and two patients with CML myelomegakaryoblast crisis who had EVI1 expression. Our results showed that EVI1 expression was frequent in patients with post-MDS AML and AML with trilineage myelodysplasia, regardless of the presence or absence of 3q26 abnormalities. EVI1 expression was accompanied by expression of GATA-1 and GATA-2, and often by stem cell leukemia (SCL) gene expression. In patients with post-MDS AML, EVI1 expression was not always associated with a 3q26 abnormality, whereas EVI1 expression in CML myelomegakaryoblast crisis was often linked to a 3q26 abnormality. Our results suggest that the leukemogenic role of EVI1 expression may differ between post-MDS AML and leukemia, with EVI1 expression associated with a 3q26 abnormality.
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evi1 expression associated with a 3q26 anomaly in a leukemia cell line derived from the blast crisis of chronic myeloid leukemia
Leukemia, 1994Co-Authors: Kazuma Ohyashiki, Takashi Shimamoto, Junko H Ohyashiki, Shinpei Nakazawa, H Fujieda, Ken Kawakubo, K Suzukawa, K Morishita, Keisuke ToyamaAbstract:Two leukemia cell lines, TS9;22 and YS9;22, were established from different individuals with Philadelphia chromosome (Ph)-positive chronic myeloid leukemia in blast crisis. The reverse transcript-polymerase chain reaction (RT-PCR) technique revealed that both cell lines expressed GATA-1, GATA-2, and the stem cell leukemia (SCL) gene, consistent with a megakaryocyte lineage. Chromosome analysis revealed that TS9;22 cells show the Ph translocation without abnormality of chromosome 3. In contrast, YS9;22 cells show the Ph translocation and dic(3)(q26;p12). Northern analysis revealed that YS9;22 cells express the EVI1 (Ecotropic Virus integration-1) gene, possibly because of the chromosomal translocation in the 3q26 region; TS9;22 cells do not express EVI1. However, no rearrangements were detected over 600 kb upstream or over 900 kb downstream of EVI1 in the YS9;22 cell line, suggesting a different mechanism of EVI1 activation from that in leukemia cells with either a t(3;3)(q21;q26) or inv(3)(q21q26). These results indicate that EVI1 expression in YS9;22 cells is linked to the 3q26 abnormality and that EVI1 activation plays an oncogenic role in the blastic transformation of chronic myeloid leukemia.
