The Experts below are selected from a list of 4764 Experts worldwide ranked by ideXlab platform

Gary D Lum - One of the best experts on this subject based on the ideXlab platform.

Ka Yin Leung - One of the best experts on this subject based on the ideXlab platform.

  • Edwardsiella tarda virulence mechanisms of an emerging gastroenteritis pathogen
    Microbes and Infection, 2012
    Co-Authors: Ka Yin Leung, Bupe A. Siame, Byron J. Tenkink, Rebecca J. Noort, Yu-keung Mok
    Abstract:

    Human Edwardsiella tarda infections often manifest as gastroenteritis, but can become systemic and potentially lethal. E. tarda uses virulence factors that include type III and type VI secretion systems, quorum sensing, two-component systems, and exoenzymes to gain entry into and survive within the host. Better understanding of interactions between these factors will lead to the development of novel antimicrobials against E. tarda and other enterics.

  • Edwardsiella tarda – Virulence mechanisms of an emerging gastroenteritis pathogen
    Microbes and infection, 2011
    Co-Authors: Ka Yin Leung, Bupe A. Siame, Byron J. Tenkink, Rebecca J. Noort, Yu-keung Mok
    Abstract:

    Human Edwardsiella tarda infections often manifest as gastroenteritis, but can become systemic and potentially lethal. E. tarda uses virulence factors that include type III and type VI secretion systems, quorum sensing, two-component systems, and exoenzymes to gain entry into and survive within the host. Better understanding of interactions between these factors will lead to the development of novel antimicrobials against E. tarda and other enterics.

  • Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.
    Infection and immunity, 2002
    Co-Authors: Y. P. Tan, Qingsong Lin, X. H. Wang, Shashikant B. Joshi, Choy L. Hew, Ka Yin Leung
    Abstract:

    A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

Yu-keung Mok - One of the best experts on this subject based on the ideXlab platform.

  • Edwardsiella tarda virulence mechanisms of an emerging gastroenteritis pathogen
    Microbes and Infection, 2012
    Co-Authors: Ka Yin Leung, Bupe A. Siame, Byron J. Tenkink, Rebecca J. Noort, Yu-keung Mok
    Abstract:

    Human Edwardsiella tarda infections often manifest as gastroenteritis, but can become systemic and potentially lethal. E. tarda uses virulence factors that include type III and type VI secretion systems, quorum sensing, two-component systems, and exoenzymes to gain entry into and survive within the host. Better understanding of interactions between these factors will lead to the development of novel antimicrobials against E. tarda and other enterics.

  • Edwardsiella tarda – Virulence mechanisms of an emerging gastroenteritis pathogen
    Microbes and infection, 2011
    Co-Authors: Ka Yin Leung, Bupe A. Siame, Byron J. Tenkink, Rebecca J. Noort, Yu-keung Mok
    Abstract:

    Human Edwardsiella tarda infections often manifest as gastroenteritis, but can become systemic and potentially lethal. E. tarda uses virulence factors that include type III and type VI secretion systems, quorum sensing, two-component systems, and exoenzymes to gain entry into and survive within the host. Better understanding of interactions between these factors will lead to the development of novel antimicrobials against E. tarda and other enterics.

Xiao-hua Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Edwardsiella tarda: an intriguing problem in aquaculture
    Aquaculture, 2014
    Co-Authors: Xiao-hua Zhang
    Abstract:

    Abstract Edwardsiella tarda is a Gram-negative intracellular pathogen accounting for enormous economic losses in cultured seawater and freshwater fish. Infection by E. tarda often leads to the development of a systematic disease called edwardsiellosis, characterized by symptoms of ascites, hernia, exophthalmia, and severe lesions of internal organs. Although edwardsiellosis has been studied for many years, however, the major virulence factors of E. tarda are still poorly understood. In addition, the control of edwardsiellosis by antibiotics or formalin-killed bacterin was not as effective as that of extracellular pathogens such as Vibrio spp. More recently, great efforts have been put in studying the virulent factors of E. tarda , especially by scientists from China, and various candidate vaccines have been developed, some of which are very promising for cure of edwardsiellosis.

  • Phenotypic diversity of Edwardsiella tarda isolated from different origins.
    Letters in applied microbiology, 2011
    Co-Authors: Yin Han, Xiaochong Shi, Xiao-hua Zhang
    Abstract:

    Aims:  To evaluate the diversity of phenotypic characteristics among isolates of Edwardsiella tarda from various origins. Methods and results:  A total of 10 E. tarda strains were investigated on biological characteristics including flagella formation, bacterial motility, biofilm formation, extracellular protein and plasmid profiles. All the E. tarda strains (including two previous recognized as nonflagellation strains) were proven to have an average of 1–7 peritrichous flagella with the precise number positively correlated with motility and biofilm formation. All the E. tarda strains exhibited similar protein profiles except ET2034, LMG2793 and ET080814, which lacked the three major bands of approximately 18, 21 and 55 kDa. E. tarda with the same geographic location shared similar plasmid profiles. Conclusions: Edwardsiella tarda strains exhibited diversities in phenotypic characteristics that may be linked to differences in geographic location or host origin. In addition, the number of flagella is essential for bacterial motility and biofilm formation. Significance and Impact of the Study:  This is the first report demonstrating the difference in flagella formation between E. tarda strains, which may broaden the understanding of flagellation trait at intra-species level. Furthermore, evaluation of virulence-associated characteristics can provide useful information for unveiling the diverse pathogenic mechanisms of E. tarda.

  • retention of virulence in a viable but nonculturable Edwardsiella tarda isolate
    Applied and Environmental Microbiology, 2007
    Co-Authors: Jixiang Chen, Xiao-hua Zhang, Yingeng Wang
    Abstract:

    Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 109 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity.

  • Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate
    Applied and environmental microbiology, 2006
    Co-Authors: Jixiang Chen, Xiao-hua Zhang, Yingeng Wang
    Abstract:

    Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (

Yingeng Wang - One of the best experts on this subject based on the ideXlab platform.

  • retention of virulence in a viable but nonculturable Edwardsiella tarda isolate
    Applied and Environmental Microbiology, 2007
    Co-Authors: Jixiang Chen, Xiao-hua Zhang, Yingeng Wang
    Abstract:

    Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (<0.1 CFU/ml) within 28 days by plate counting. The direct viable counts, on the other hand, declined to ca. 109 CFU/ml active cells and remained fairly constant at this level by direct viable counting. These results indicated that a large population of cells existed in a viable but nonculturable state. VBNC E. tarda CW7 could resuscitate in experimental chick embryos and in the presence of nutrition with a temperature upshift. The resuscitative times were 6 days and 8 days, respectively. The morphological changes of VBNC, normal, and resuscitative E. tarda CW7 cells were studied with a scanning electron microscope. The results showed that when the cells entered into the VBNC state, they gradually changed in shape from short rods to coccoid and decreased in size, but the resuscitative cells did not show any obvious differences from the normal cells. The VBNC and the resuscitative E. tarda CW7 cells were intraperitoneally inoculated into turbot separately, and the fish inoculated with the resuscitative cells died within 7 days, which suggested that VBNC E. tarda CW7 might retain pathogenicity.

  • Retention of Virulence in a Viable but Nonculturable Edwardsiella tarda Isolate
    Applied and environmental microbiology, 2006
    Co-Authors: Jixiang Chen, Xiao-hua Zhang, Yingeng Wang
    Abstract:

    Edwardsiella tarda is pathogen of fish and other animals. The aim of this study was to investigate the viable but nonculturable (VBNC) state and virulence retention of this bacterium. Edwardsiella tarda CW7 was cultured in sterilized aged seawater at 4°C. Total cell counts remained constant throughout the 28-day period by acridine orange direct counting, while plate counts declined to undetectable levels (