The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform

Julien Bobe - One of the best experts on this subject based on the ideXlab platform.

  • Transcriptomic profiling of Eggs of pikeperch (Sander lucioperca) reveals novel Egg-Quality-associated transcripts
    2019
    Co-Authors: Aurélie Le Cam, Christophe Klopp, Jérôme Montfort, Pascal Fontaine, J. Nynca, S. Ciesielski, A. Ciereszko, Julien Bobe
    Abstract:

    Transcriptomic profiling of Eggs of pikeperch (Sander lucioperca) reveals novel Egg-Quality-associated transcripts. Aquaculture Europe 2019

  • Transcriptomic profiling reveals novel biomarkers of Egg Quality in domesticated pikeperch, Sander lucioperca
    2019
    Co-Authors: Daniel Zarski, Aurélie Le Cam, Christophe Klopp, Pascal Fontaine, Joanna Nynca, Sławomir Ciesielski, Andrzej Ciereszko, Julien Bobe
    Abstract:

    Transcriptomic profiling reveals novel biomarkers of Egg Quality in domesticated pikeperch, Sander lucioperca. 7. International Workshop on the Biology of Fish Gametes

  • Difficulties to compare Egg Quality: insights from a transcriptomic analysis
    2019
    Co-Authors: Taina Rocha De Almeida, Julien Bobe, Aurélie Le Cam, Christophe Klopp, Jérôme Montfort, Pascal Fontaine, Dominique Chardard, Bérénice Schaerlinger
    Abstract:

    Difficulties to compare Egg Quality: insights from a transcriptomic analysis. 7. International Workshop on the Biology of Fish Gametes

  • Molecular portrait of Egg Quality and novel maternal-effect genes
    2018
    Co-Authors: Caroline Cheung, Aurélie Le Cam, Thuy Thao Vi Nguyen, Amélie Patinote, D. Zarski, Laurent Journot, Christelle Reynes, Julien Bobe
    Abstract:

    Good Quality or developmentally competent fish Eggs are successfully fertilized and develop normally as viable, non-malformed embryos. However, the detailed mechanisms involved in Egg Quality remain poorly understood and, at present, no predictive markers of Egg Quality exist. Early developmental success relies on maternally-inherited molecules deposited in the oocyte throughout oogenesis, and over the last few years, our work has aimed at drawing the molecular picture of poorly competent (i.e. bad Quality) fish Eggs in order to (1) further uncover the molecular mechanisms associated with low developmental success, (2) reveal new maternal-effect genes that play important roles during early development, and (3) possibly obtain molecular signatures based on maternal mRNA profiles in fertilized Eggs that could be used as predictive markers of good Quality Eggs. A large transcriptomic analysis was performed in zebrafish (Danio rerio) using a high number of Egg clutches obtained under normal husbandry conditions that reflect naturally occurring variability in Egg Quality. The differentially expressed gene profile revealed dysregulation of genes involved in translation and protein synthesis. Interestingly, translation was also one of the main processes dysregulated in a similar study conducted in Sea Bass (Dicentrarchus labrax). Analysis of a smaller number of zebrafish females that consistently produced bad Eggs demonstrated a much lower variability in expression profiles that resulted in a higher number of differentially expressed genes identified between good and bad Quality Eggs. These indicate that while common/general traits of bad Quality Egg exist, a multiplicity of other factors can dysregulate specific processes that ultimately lead to reduced Egg Quality. To gain insight into their functions, genome editing using the CRISPR/Cas9 technology was performed to create mutants of two new potential maternal-effect genes, otulina (OTU deubiquitinase with linear linkage specificity a) and slc29a1a (solute carrier family 29, member 1a), that are especially abundant in the ovary. Due to the high efficiency of this technique, mutations could not be transmitted to the next generation and the phenotype was analyzed in F0 females. We observed that both otulina and slc29a1a mutant-derived Eggs had very low developmental success. Eggs from mutant females crossed with vasa:eGFP males do not contain GFP, suggesting that they cannot be fertilized. These novel findings showed for the first time that otulina and slc29a1a are essential for the developmental competence of Eggs, therefore, are crucial maternal-effect genes. Finally, we applied statistical modeling using Partial Least Square (PLS) regression and genetic algorithm to our data to search for possible molecular signatures. We observed the presence of strong gene signatures, which were statistically robust both in terms of reproducibility and validation by pseudo-data, to link gene expression to the survival rate of Eggs in our transcriptomic data. In summary, the molecular mechanisms that control Egg Quality are complex and probably variable even though some core processes including translation and proteins synthesis appear to play a key role. Yet, some maternaleffect genes have been identified that are important for fertilization. Finally, it appears possible to statistically predict developmental success based on the molecular portrait of the Egg.

  • Maternal-effect genes and Egg Quality: usual suspects and new players
    2017
    Co-Authors: Julien Bobe, Violette Thermes
    Abstract:

    Maternal-effect genes and Egg Quality: usual suspects and new players. 6. International Workshop on the Biology of Fish Gametes

Aurélie Le Cam - One of the best experts on this subject based on the ideXlab platform.

  • Transcriptomic profiling of Eggs of pikeperch (Sander lucioperca) reveals novel Egg-Quality-associated transcripts
    2019
    Co-Authors: Aurélie Le Cam, Christophe Klopp, Jérôme Montfort, Pascal Fontaine, J. Nynca, S. Ciesielski, A. Ciereszko, Julien Bobe
    Abstract:

    Transcriptomic profiling of Eggs of pikeperch (Sander lucioperca) reveals novel Egg-Quality-associated transcripts. Aquaculture Europe 2019

  • Difficulties to compare Egg Quality: insights from a transcriptomic analysis
    2019
    Co-Authors: Taina Rocha De Almeida, Julien Bobe, Aurélie Le Cam, Christophe Klopp, Jérôme Montfort, Pascal Fontaine, Dominique Chardard, Bérénice Schaerlinger
    Abstract:

    Difficulties to compare Egg Quality: insights from a transcriptomic analysis. 7. International Workshop on the Biology of Fish Gametes

  • Transcriptomic profiling reveals novel biomarkers of Egg Quality in domesticated pikeperch, Sander lucioperca
    2019
    Co-Authors: Daniel Zarski, Aurélie Le Cam, Christophe Klopp, Pascal Fontaine, Joanna Nynca, Sławomir Ciesielski, Andrzej Ciereszko, Julien Bobe
    Abstract:

    Transcriptomic profiling reveals novel biomarkers of Egg Quality in domesticated pikeperch, Sander lucioperca. 7. International Workshop on the Biology of Fish Gametes

  • Molecular portrait of Egg Quality and novel maternal-effect genes
    2018
    Co-Authors: Caroline Cheung, Aurélie Le Cam, Thuy Thao Vi Nguyen, Amélie Patinote, D. Zarski, Laurent Journot, Christelle Reynes, Julien Bobe
    Abstract:

    Good Quality or developmentally competent fish Eggs are successfully fertilized and develop normally as viable, non-malformed embryos. However, the detailed mechanisms involved in Egg Quality remain poorly understood and, at present, no predictive markers of Egg Quality exist. Early developmental success relies on maternally-inherited molecules deposited in the oocyte throughout oogenesis, and over the last few years, our work has aimed at drawing the molecular picture of poorly competent (i.e. bad Quality) fish Eggs in order to (1) further uncover the molecular mechanisms associated with low developmental success, (2) reveal new maternal-effect genes that play important roles during early development, and (3) possibly obtain molecular signatures based on maternal mRNA profiles in fertilized Eggs that could be used as predictive markers of good Quality Eggs. A large transcriptomic analysis was performed in zebrafish (Danio rerio) using a high number of Egg clutches obtained under normal husbandry conditions that reflect naturally occurring variability in Egg Quality. The differentially expressed gene profile revealed dysregulation of genes involved in translation and protein synthesis. Interestingly, translation was also one of the main processes dysregulated in a similar study conducted in Sea Bass (Dicentrarchus labrax). Analysis of a smaller number of zebrafish females that consistently produced bad Eggs demonstrated a much lower variability in expression profiles that resulted in a higher number of differentially expressed genes identified between good and bad Quality Eggs. These indicate that while common/general traits of bad Quality Egg exist, a multiplicity of other factors can dysregulate specific processes that ultimately lead to reduced Egg Quality. To gain insight into their functions, genome editing using the CRISPR/Cas9 technology was performed to create mutants of two new potential maternal-effect genes, otulina (OTU deubiquitinase with linear linkage specificity a) and slc29a1a (solute carrier family 29, member 1a), that are especially abundant in the ovary. Due to the high efficiency of this technique, mutations could not be transmitted to the next generation and the phenotype was analyzed in F0 females. We observed that both otulina and slc29a1a mutant-derived Eggs had very low developmental success. Eggs from mutant females crossed with vasa:eGFP males do not contain GFP, suggesting that they cannot be fertilized. These novel findings showed for the first time that otulina and slc29a1a are essential for the developmental competence of Eggs, therefore, are crucial maternal-effect genes. Finally, we applied statistical modeling using Partial Least Square (PLS) regression and genetic algorithm to our data to search for possible molecular signatures. We observed the presence of strong gene signatures, which were statistically robust both in terms of reproducibility and validation by pseudo-data, to link gene expression to the survival rate of Eggs in our transcriptomic data. In summary, the molecular mechanisms that control Egg Quality are complex and probably variable even though some core processes including translation and proteins synthesis appear to play a key role. Yet, some maternaleffect genes have been identified that are important for fertilization. Finally, it appears possible to statistically predict developmental success based on the molecular portrait of the Egg.

Pascal Fontaine - One of the best experts on this subject based on the ideXlab platform.

Christophe Klopp - One of the best experts on this subject based on the ideXlab platform.

Ivan Miksik - One of the best experts on this subject based on the ideXlab platform.

  • Egg pigmentation reflects female and Egg Quality in the spotless starling Sturnus unicolor
    Behavioral Ecology and Sociobiology, 2008
    Co-Authors: Isabel López-rull, Ivan Miksik
    Abstract:

    It has been proposed that blue colouration in Eggs has evolved as a signal of female Quality that males can use to modulate their parental investment. This hypothesis is based in the antioxidant properties of biliverdin whose costly deposition in the Eggshell is expected to signal female antioxidant capacity and Egg Quality. Since maternally derived androgens are costly to produce and may adaptively affect offspring phenotype, high-Quality females may benefit by signalling their androgen investment through Egg colouration. Our aim was to investigate whether Egg colour variation in the spotless starling reflected the amount of pigments on the Eggshell and whether Egg pigmentation was related to female and Egg Quality. Chromatography analyses revealed that spotless starling Eggshells contained two different pigments: biliverdin and protoporphyrin IX with no correlation between them. Biliverdin contents correlated positively with Egg colouration indicating that darker Eggs with a higher peak in the blue–green segment of the spectrum contained higher amounts of biliverdin. Eggs containing more biliverdin were laid by high-Quality females and contained higher yolk testosterone levels. However, despite the strong correlation between biliverdin and colorimetric variables, Egg colouration did not reflect accurately female and Egg Quality. Our results provide evidence that Eggshell pigmentation in the spotless starling is related to female and Egg Quality as shown by the yolk testosterone levels. However, the lack of relation between Egg colour and female condition and Egg Quality do not provide evidence to support the signalling function of Egg colouration.