The Experts below are selected from a list of 252 Experts worldwide ranked by ideXlab platform

Charles A. Dimarzio - One of the best experts on this subject based on the ideXlab platform.

  • Phase-subtraction cell-counting method for live mouse embryos beyond the Eight-Cell Stage
    Journal of biomedical optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    Since 1978 in vitro fertilization IVF procedures have re- sulted in the birth of over 3 million babies. Yet in 2005, IVF proce- dures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to increase the probability that a single, healthy embryo was included. The predomi- nantly accepted noninvasive viability markers for embryos created by IVF are 1 number of cells at specific time points during development and 2 overall morphology of the embryo. Currently, it is difficult to count the number of cells beyond the Eight-Cell Stage noninvasively. We report a nontoxic cell-counting method capable of counting cell numbers ranging from 8 to 26 in live mouse embryos. This method is derived from the fusion of differential interference contrast and optical quadrature microscopy and is verified by epifluorescence images of Hoechst-stained nuclei. The phase-subtraction cell-counting method is the first accurate, nontoxic technique to count cells through the morula Stage in mouse embryos and may enhance the use of cell number as a viability marker if adopted for use with human embryos in the IVF clinic. © 2008 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2937468

  • Phase subtraction cell counting method and dry mass determination for assessment of viability of mouse embryos
    Biomedical Optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. Here we describe the combination of the cell count and dry mass to assess viability.

  • Phase Subtraction Cell Counting Method for Live Mouse Embryos
    Biomedical Optics, 2006
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. This method could revolutionize the clinician's ability to determine embryo viability for IVF procedures.

William C. Warger - One of the best experts on this subject based on the ideXlab platform.

  • Phase-subtraction cell-counting method for live mouse embryos beyond the Eight-Cell Stage
    Journal of biomedical optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    Since 1978 in vitro fertilization IVF procedures have re- sulted in the birth of over 3 million babies. Yet in 2005, IVF proce- dures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to increase the probability that a single, healthy embryo was included. The predomi- nantly accepted noninvasive viability markers for embryos created by IVF are 1 number of cells at specific time points during development and 2 overall morphology of the embryo. Currently, it is difficult to count the number of cells beyond the Eight-Cell Stage noninvasively. We report a nontoxic cell-counting method capable of counting cell numbers ranging from 8 to 26 in live mouse embryos. This method is derived from the fusion of differential interference contrast and optical quadrature microscopy and is verified by epifluorescence images of Hoechst-stained nuclei. The phase-subtraction cell-counting method is the first accurate, nontoxic technique to count cells through the morula Stage in mouse embryos and may enhance the use of cell number as a viability marker if adopted for use with human embryos in the IVF clinic. © 2008 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2937468

  • Phase subtraction cell counting method and dry mass determination for assessment of viability of mouse embryos
    Biomedical Optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. Here we describe the combination of the cell count and dry mass to assess viability.

  • Phase Subtraction Cell Counting Method for Live Mouse Embryos
    Biomedical Optics, 2006
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. This method could revolutionize the clinician's ability to determine embryo viability for IVF procedures.

Thomas M. Dechiara - One of the best experts on this subject based on the ideXlab platform.

  • Injecting Embryonic Stem Cells into Eight-Cell-Stage Mouse Embryos.
    Cold Spring Harbor protocols, 2017
    Co-Authors: Wojtek Auerbach, Thomas M. Dechiara
    Abstract:

    In this protocol, Eight-Cell-Stage precompaction embryos from outbred mouse strains are used for the injection of hybrid or inbred embryonic stem (ES) cells. This process often leads to generation of fully ES cell-derived so-called F0 mice (VelociMice). Postinjection culture of embryos is necessary to achieve the highest ratio of fully ES cell-derived mice and high-degree chimeras. Typically, 50 embryos are injected per ES cell clone.

  • producing fully es cell derived mice from eight cell Stage embryo injections
    Methods in Enzymology, 2010
    Co-Authors: Thomas M. Dechiara, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, William Poueymirou, David M. Valenzuela
    Abstract:

    Abstract In conventional methods for the generation of genetically modified mice, gene-targeted embryonic stem (ES) cells are injected into blastocyst-Stage embryos or are aggregated with morula-Stage embryos, which are then transferred to the uterus of a surrogate mother. F0 generation mice born from the embryos are chimeras composed of genetic contributions from both the modified ES cells and the recipient embryos. Obtaining a mouse strain that carries the gene-targeted mutation requires breeding the chimeras to transmit the ES cell genetic component through the germ line to the next (F1) generation (germ line transmission, GLT). To skip the chimera Stage, we developed the VelociMouse® method, in which injection of genetically modified ES cells into Eight-Cell embryos followed by maturation to the blastocyst Stage and transfer to a surrogate mother produces F0 generation mice that are fully derived from the injected ES cells and exhibit a 100% GLT efficiency. The method is simple and flexible. Both male and female ES cells can be introduced into the Eight-Cell embryo by any method of injection or aggregation and using all ES cell and host embryo combinations from inbred, hybrid, and outbred genetic backgrounds. The VelociMouse® method provides several unique opportunities for shortening project timelines and reducing mouse husbandry costs. First, as VelociMice® exhibit 100% GLT, there is no need to test cross chimeras to establish GLT. Second, because the VelociMouse method permits efficient production of ES cell-derived mice from female ES cells, XO ES cell subclones, identified by screening for spontaneous loss of the Y chromosome, can be used to generate F0 females that can be bred with isogenic F0 males derived from the original targeted ES cell clone to obtain homozygous mutant mice in the F1 generation. Third, as VelociMice are genetically identical to the ES cells from which they were derived, the VelociMouse method opens up myriad possibilities for creating mice with complex genotypes in a defined genetic background directly from engineered ES cells without the need for inefficient and lengthy breeding schemes. Examples include creation of F0 knockout mice from ES cells carrying a homozygous null mutation, and creation of a mouse with a tissue-specific gene inactivation by combining null and floxed conditional alleles for the target gene with a transgenic Cre recombinase allele controlled by a tissue-specific promoter. VelociMice with the combinatorial alleles are ready for immediate phenotypic studies, which greatly accelerates gene function assignment and the creation of valuable models of human disease.

  • VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos
    Methods in molecular biology (Clifton N.J.), 2009
    Co-Authors: Thomas M. Dechiara, Wojtek Auerbach, William T. Poueymirou, David Frendewey, George D. Yancopoulos, David M. Valenzuela
    Abstract:

    With the completion of the human and mouse genome sequences and the development of high-throughput knockout mouse technologies, there is now a need for equally high-throughput methods for the production of mice for phenotypic studies. In response to this challenge, we recently developed a new method termed VelociMouse for the production of F0-generation mice that are fully derived from gene-targeted ES cells. In the version of the VelociMouse method described here, laser ablation of a portion of the zona pellucid (zp) of a normal Eight-Cell-Stage embryo facilitates ES cell injection. Upon gestation in a surrogate mother, the injected embryos produce F0 mice that carry no detectable host embryo contribution (

Carol M. Warner - One of the best experts on this subject based on the ideXlab platform.

  • Phase-subtraction cell-counting method for live mouse embryos beyond the Eight-Cell Stage
    Journal of biomedical optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    Since 1978 in vitro fertilization IVF procedures have re- sulted in the birth of over 3 million babies. Yet in 2005, IVF proce- dures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to increase the probability that a single, healthy embryo was included. The predomi- nantly accepted noninvasive viability markers for embryos created by IVF are 1 number of cells at specific time points during development and 2 overall morphology of the embryo. Currently, it is difficult to count the number of cells beyond the Eight-Cell Stage noninvasively. We report a nontoxic cell-counting method capable of counting cell numbers ranging from 8 to 26 in live mouse embryos. This method is derived from the fusion of differential interference contrast and optical quadrature microscopy and is verified by epifluorescence images of Hoechst-stained nuclei. The phase-subtraction cell-counting method is the first accurate, nontoxic technique to count cells through the morula Stage in mouse embryos and may enhance the use of cell number as a viability marker if adopted for use with human embryos in the IVF clinic. © 2008 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2937468

  • Phase subtraction cell counting method and dry mass determination for assessment of viability of mouse embryos
    Biomedical Optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. Here we describe the combination of the cell count and dry mass to assess viability.

  • Phase Subtraction Cell Counting Method for Live Mouse Embryos
    Biomedical Optics, 2006
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. This method could revolutionize the clinician's ability to determine embryo viability for IVF procedures.

Judith A. Newmark - One of the best experts on this subject based on the ideXlab platform.

  • Phase-subtraction cell-counting method for live mouse embryos beyond the Eight-Cell Stage
    Journal of biomedical optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    Since 1978 in vitro fertilization IVF procedures have re- sulted in the birth of over 3 million babies. Yet in 2005, IVF proce- dures had a live birth rate of only 34%, with 32% of these births resulting in multiple pregnancies. These multiple pregnancies were directly attributed to the transfer of multiple embryos to increase the probability that a single, healthy embryo was included. The predomi- nantly accepted noninvasive viability markers for embryos created by IVF are 1 number of cells at specific time points during development and 2 overall morphology of the embryo. Currently, it is difficult to count the number of cells beyond the Eight-Cell Stage noninvasively. We report a nontoxic cell-counting method capable of counting cell numbers ranging from 8 to 26 in live mouse embryos. This method is derived from the fusion of differential interference contrast and optical quadrature microscopy and is verified by epifluorescence images of Hoechst-stained nuclei. The phase-subtraction cell-counting method is the first accurate, nontoxic technique to count cells through the morula Stage in mouse embryos and may enhance the use of cell number as a viability marker if adopted for use with human embryos in the IVF clinic. © 2008 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.2937468

  • Phase subtraction cell counting method and dry mass determination for assessment of viability of mouse embryos
    Biomedical Optics, 2008
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. Here we describe the combination of the cell count and dry mass to assess viability.

  • Phase Subtraction Cell Counting Method for Live Mouse Embryos
    Biomedical Optics, 2006
    Co-Authors: William C. Warger, Judith A. Newmark, Carol M. Warner, Charles A. Dimarzio
    Abstract:

    The phase subtraction cell counting method has produced accurate, non-toxic cell counts in live mouse embryos beyond the Eight-Cell Stage. This method could revolutionize the clinician's ability to determine embryo viability for IVF procedures.